Marisediminitalea aggregata

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Marisediminitalea

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4313 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5HVM5|A0A1M5HVM5_9ALTE Transporter SSS family OS=Marisediminitalea aggregata OX=634436 GN=SAMN05216361_1638 PE=3 SV=1
MM1 pKa = 7.46KK2 pKa = 9.94IKK4 pKa = 10.79YY5 pKa = 7.57PALISAMWASQVFATDD21 pKa = 2.96IFINEE26 pKa = 3.87LHH28 pKa = 6.48YY29 pKa = 11.25DD30 pKa = 3.51NSGGDD35 pKa = 3.0VGEE38 pKa = 4.27FVEE41 pKa = 5.26VVAPAGTDD49 pKa = 3.21LTGYY53 pKa = 10.34KK54 pKa = 10.22VLFYY58 pKa = 10.74NGSNGTQYY66 pKa = 11.36NSRR69 pKa = 11.84EE70 pKa = 4.16LVGLVADD77 pKa = 4.29MGNGFGTYY85 pKa = 10.08AIDD88 pKa = 3.96VSGIQNGAPDD98 pKa = 4.28GVALVDD104 pKa = 3.72ASSNVIQFLSYY115 pKa = 10.33EE116 pKa = 4.27GSFTATAGVLAGLTSVDD133 pKa = 3.02IGVSQSSSTPAGASLQLTGTGAQYY157 pKa = 11.48SDD159 pKa = 3.99FTWQAFTTATKK170 pKa = 10.6DD171 pKa = 3.37AVNEE175 pKa = 4.23GQTFTGNEE183 pKa = 3.63VVFFNEE189 pKa = 3.07IHH191 pKa = 6.42YY192 pKa = 10.81DD193 pKa = 3.52NAGGDD198 pKa = 3.25VGEE201 pKa = 4.96FIEE204 pKa = 4.5IAGTAGLDD212 pKa = 3.51LTGMTIEE219 pKa = 5.47LYY221 pKa = 10.4NGSNGTVYY229 pKa = 9.63NTIVLSGALPDD240 pKa = 3.85NGNGFGTIAYY250 pKa = 8.48YY251 pKa = 10.02PSSIQNGAPDD261 pKa = 3.75GMALVSATGEE271 pKa = 4.19VIQFLSYY278 pKa = 10.61EE279 pKa = 4.4GVVSATAGAAVGTDD293 pKa = 3.24STDD296 pKa = 2.67IGIAEE301 pKa = 4.39TSSTAIGQSLQLSGTGTRR319 pKa = 11.84YY320 pKa = 10.44SDD322 pKa = 3.65FTWIAGGEE330 pKa = 4.4TPDD333 pKa = 3.81NTNASQTFEE342 pKa = 4.18GVGGGTPEE350 pKa = 3.97EE351 pKa = 4.4PEE353 pKa = 4.26EE354 pKa = 4.44PEE356 pKa = 4.22EE357 pKa = 4.08PTPGTGDD364 pKa = 3.4VAKK367 pKa = 10.93LFINEE372 pKa = 3.94LHH374 pKa = 6.5YY375 pKa = 11.52DD376 pKa = 3.66NASSDD381 pKa = 3.23VGEE384 pKa = 4.37FVEE387 pKa = 4.41IAGPAGTDD395 pKa = 3.27LTGVSIEE402 pKa = 4.64LYY404 pKa = 9.51NGNGGGVYY412 pKa = 8.68KK413 pKa = 10.27TVALSGTIPEE423 pKa = 4.17QAGGFGTVAEE433 pKa = 4.82YY434 pKa = 10.41ISGIQNGAPDD444 pKa = 4.66GVALSYY450 pKa = 10.93NGEE453 pKa = 4.39LIDD456 pKa = 4.44FISYY460 pKa = 10.18EE461 pKa = 4.32GEE463 pKa = 3.82LTATGGSASGVTATDD478 pKa = 2.63IGVAEE483 pKa = 4.38TGSTLSTEE491 pKa = 4.58SVQLTGTGSEE501 pKa = 4.21VCHH504 pKa = 6.31FSWSGPATSSPGEE517 pKa = 3.97INAGQTFDD525 pKa = 3.67ASVGATCGGNDD536 pKa = 4.05GGDD539 pKa = 3.29GGTGGEE545 pKa = 4.19VTLVKK550 pKa = 10.18ISEE553 pKa = 4.38VQGSGSSSPLINAQVAIEE571 pKa = 4.64AIVVADD577 pKa = 3.97HH578 pKa = 6.24QSGSGTDD585 pKa = 3.18GDD587 pKa = 3.86LRR589 pKa = 11.84GFFVQEE595 pKa = 3.56EE596 pKa = 4.23DD597 pKa = 3.65ADD599 pKa = 4.06ADD601 pKa = 4.07ADD603 pKa = 3.95SSTSEE608 pKa = 3.9GLFVFDD614 pKa = 4.65GSSPAVDD621 pKa = 3.33VAVGDD626 pKa = 3.67KK627 pKa = 10.6VYY629 pKa = 11.07LVGTVDD635 pKa = 3.99EE636 pKa = 4.82YY637 pKa = 11.51FGDD640 pKa = 3.78TQLDD644 pKa = 3.95VQSGSVQVVSSGNALPSPAIVTLPLATTTNSGGEE678 pKa = 4.12AIADD682 pKa = 3.98LEE684 pKa = 4.61AYY686 pKa = 10.0EE687 pKa = 4.61GMQVMFAQTLAVTEE701 pKa = 4.48LFNLDD706 pKa = 3.23RR707 pKa = 11.84FGEE710 pKa = 4.15ILLSSEE716 pKa = 3.9GRR718 pKa = 11.84QVQYY722 pKa = 10.61TEE724 pKa = 3.61IMTPSVEE731 pKa = 4.28GYY733 pKa = 9.5AAYY736 pKa = 10.23VASAAANRR744 pKa = 11.84LMLDD748 pKa = 3.95DD749 pKa = 5.41GFSIQNPSPIRR760 pKa = 11.84FPAPGLSTANTVRR773 pKa = 11.84MSDD776 pKa = 3.18EE777 pKa = 4.18VTNLTGVISYY787 pKa = 10.3RR788 pKa = 11.84RR789 pKa = 11.84GSGGSGDD796 pKa = 3.3EE797 pKa = 4.64MYY799 pKa = 11.26RR800 pKa = 11.84LMPTVEE806 pKa = 4.2PVFTRR811 pKa = 11.84PEE813 pKa = 4.0SRR815 pKa = 11.84KK816 pKa = 9.97DD817 pKa = 3.5RR818 pKa = 11.84PDD820 pKa = 3.13VGGSIQVASFNLLNFFNTFGNSCYY844 pKa = 10.41LGGGTGDD851 pKa = 4.61CRR853 pKa = 11.84GADD856 pKa = 3.3NTEE859 pKa = 3.96EE860 pKa = 4.72YY861 pKa = 10.73DD862 pKa = 3.59RR863 pKa = 11.84QLQKK867 pKa = 9.98TVTAITALDD876 pKa = 3.83ADD878 pKa = 3.99VLGVIEE884 pKa = 4.5IEE886 pKa = 3.86NDD888 pKa = 2.9IVEE891 pKa = 4.61GQNSAMASLVNALNQAGSACSDD913 pKa = 3.55YY914 pKa = 11.19QYY916 pKa = 11.28VDD918 pKa = 3.16PQQRR922 pKa = 11.84VGTDD926 pKa = 3.95AIAVGFLYY934 pKa = 10.84CADD937 pKa = 3.9TVEE940 pKa = 4.46VTAGSSIAILDD951 pKa = 3.99DD952 pKa = 3.73SQLAGLGLSDD962 pKa = 4.09YY963 pKa = 11.42APVFDD968 pKa = 5.04GSSTSRR974 pKa = 11.84PSLTVSFTEE983 pKa = 4.17KK984 pKa = 9.81ATSEE988 pKa = 4.27VFTVSVNHH996 pKa = 6.4FKK998 pKa = 11.19SKK1000 pKa = 10.51GGSGSGGNADD1010 pKa = 4.34ANDD1013 pKa = 4.2GAGNWNEE1020 pKa = 3.8RR1021 pKa = 11.84RR1022 pKa = 11.84TQAAEE1027 pKa = 4.13ALHH1030 pKa = 6.59KK1031 pKa = 10.11WLATYY1036 pKa = 7.59PTGVSDD1042 pKa = 6.11DD1043 pKa = 5.08DD1044 pKa = 3.59ILIIGDD1050 pKa = 4.08LNAYY1054 pKa = 10.38SMEE1057 pKa = 4.26DD1058 pKa = 3.69PIVEE1062 pKa = 4.23LTSNGYY1068 pKa = 8.01TNVTGGEE1075 pKa = 3.67AHH1077 pKa = 6.19YY1078 pKa = 10.81SYY1080 pKa = 11.49VFDD1083 pKa = 4.39GFAGNLDD1090 pKa = 3.84HH1091 pKa = 7.1GLASASLMDD1100 pKa = 4.6QITGATSWNINSDD1113 pKa = 3.36EE1114 pKa = 4.75ADD1116 pKa = 3.46ALDD1119 pKa = 3.78YY1120 pKa = 10.35NTDD1123 pKa = 3.76FGRR1126 pKa = 11.84PTDD1129 pKa = 3.76IFDD1132 pKa = 3.45GTTPYY1137 pKa = 9.19RR1138 pKa = 11.84TSDD1141 pKa = 3.79HH1142 pKa = 7.29DD1143 pKa = 3.93PLLIGLSLSSVLKK1156 pKa = 10.79GDD1158 pKa = 3.97LDD1160 pKa = 4.21GDD1162 pKa = 4.04GDD1164 pKa = 4.11IDD1166 pKa = 4.79RR1167 pKa = 11.84MDD1169 pKa = 5.65FILIMRR1175 pKa = 11.84AILTRR1180 pKa = 11.84KK1181 pKa = 9.6PFDD1184 pKa = 3.48AANDD1188 pKa = 3.65INGDD1192 pKa = 3.78GVVNIVDD1199 pKa = 3.65GLLLRR1204 pKa = 11.84RR1205 pKa = 11.84SCTRR1209 pKa = 11.84ARR1211 pKa = 11.84CAVRR1215 pKa = 4.07

Molecular weight: 125.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5GDS0|A0A1M5GDS0_9ALTE Endonuclease III OS=Marisediminitalea aggregata OX=634436 GN=nth PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.14RR12 pKa = 11.84KK13 pKa = 9.13RR14 pKa = 11.84SHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37NGRR28 pKa = 11.84KK29 pKa = 9.16VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.29GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4313

0

4313

1469709

29

2683

340.8

37.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.415 ± 0.039

0.99 ± 0.012

5.801 ± 0.031

5.902 ± 0.035

4.138 ± 0.025

6.895 ± 0.038

2.229 ± 0.021

5.789 ± 0.027

4.485 ± 0.03

10.138 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.493 ± 0.016

4.185 ± 0.027

4.159 ± 0.022

4.717 ± 0.032

4.625 ± 0.026

6.577 ± 0.029

5.729 ± 0.03

7.229 ± 0.032

1.327 ± 0.013

3.179 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski