Flavimobilis marinus
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2643 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I2GLJ4|A0A1I2GLJ4_9MICO Chemotaxis phosphatase CheX OS=Flavimobilis marinus OX=285351 GN=SAMN04488035_1851 PE=4 SV=1
MM1 pKa = 6.96 TAAGTPRR8 pKa = 11.84 RR9 pKa = 11.84 SLRR12 pKa = 11.84 AVGAIAALGLVLTACADD29 pKa = 3.56 TSEE32 pKa = 5.38 GDD34 pKa = 3.86 DD35 pKa = 3.56 PTATGGTAGGGTDD48 pKa = 4.0 SISVGTTDD56 pKa = 4.96 VITSLDD62 pKa = 3.58 PAGSYY67 pKa = 11.34 DD68 pKa = 3.46 NGSFAVQNQVFPFLLNTPYY87 pKa = 10.83 GSPDD91 pKa = 3.56 VEE93 pKa = 3.89 PDD95 pKa = 3.01 IAEE98 pKa = 4.28 SAEE101 pKa = 3.81 FTAPNEE107 pKa = 3.95 YY108 pKa = 9.34 TVTLKK113 pKa = 10.98 EE114 pKa = 4.14 GLTFANGHH122 pKa = 6.04 EE123 pKa = 4.48 LTSSDD128 pKa = 3.4 VKK130 pKa = 10.92 FSFDD134 pKa = 3.51 RR135 pKa = 11.84 QLGIADD141 pKa = 3.9 PNGPSSLLYY150 pKa = 10.92 NLDD153 pKa = 3.31 SVAAPDD159 pKa = 3.39 EE160 pKa = 4.27 RR161 pKa = 11.84 TVVFTLKK168 pKa = 10.58 SEE170 pKa = 4.16 NDD172 pKa = 3.17 QTFPQILSSPVGPIVDD188 pKa = 3.95 EE189 pKa = 4.62 EE190 pKa = 4.69 VFSPTEE196 pKa = 3.84 LTPADD201 pKa = 3.76 TIVAEE206 pKa = 4.39 KK207 pKa = 10.95 AFGGQYY213 pKa = 10.75 LITDD217 pKa = 3.57 YY218 pKa = 11.33 TEE220 pKa = 4.27 NEE222 pKa = 4.42 LIRR225 pKa = 11.84 YY226 pKa = 7.48 EE227 pKa = 4.13 AFEE230 pKa = 5.97 DD231 pKa = 4.04 YY232 pKa = 10.32 MGLLEE237 pKa = 4.19 PAVTKK242 pKa = 10.71 VVTAQYY248 pKa = 8.37 FTEE251 pKa = 4.25 EE252 pKa = 4.15 TSLKK256 pKa = 10.64 LAVQQGDD263 pKa = 3.15 VDD265 pKa = 3.86 VAFRR269 pKa = 11.84 SLSPTDD275 pKa = 4.08 LADD278 pKa = 4.08 LAQDD282 pKa = 3.57 EE283 pKa = 4.74 NVTVHH288 pKa = 6.82 EE289 pKa = 5.04 GPGGEE294 pKa = 3.43 IRR296 pKa = 11.84 YY297 pKa = 9.32 IVFNFATQPYY307 pKa = 8.21 GTDD310 pKa = 2.91 TADD313 pKa = 3.45 ADD315 pKa = 3.92 EE316 pKa = 4.82 AKK318 pKa = 10.76 ALAVRR323 pKa = 11.84 QAAAHH328 pKa = 5.97 LLDD331 pKa = 3.81 RR332 pKa = 11.84 QALSEE337 pKa = 4.41 EE338 pKa = 4.78 IYY340 pKa = 10.59 QGSFTPLYY348 pKa = 10.57 SYY350 pKa = 11.35 VPDD353 pKa = 4.28 GLTGAAEE360 pKa = 4.12 PLKK363 pKa = 10.47 EE364 pKa = 4.39 LYY366 pKa = 10.64 GDD368 pKa = 3.79 GAGAPDD374 pKa = 4.2 ADD376 pKa = 3.81 AAAAALSEE384 pKa = 4.67 AGVEE388 pKa = 4.35 TPVALNLQYY397 pKa = 11.28 SPDD400 pKa = 3.57 HH401 pKa = 6.48 YY402 pKa = 11.26 GNSSDD407 pKa = 4.48 EE408 pKa = 4.18 EE409 pKa = 4.34 YY410 pKa = 11.85 AMIEE414 pKa = 4.13 AQLEE418 pKa = 4.08 ASGLFDD424 pKa = 4.59 VSLQSTLWDD433 pKa = 3.61 TYY435 pKa = 7.98 STEE438 pKa = 3.86 RR439 pKa = 11.84 RR440 pKa = 11.84 EE441 pKa = 4.39 DD442 pKa = 3.43 VYY444 pKa = 10.88 PAYY447 pKa = 10.28 QLGWFPDD454 pKa = 3.67 YY455 pKa = 11.48 SDD457 pKa = 3.97 ADD459 pKa = 3.75 NYY461 pKa = 11.0 LSPFFLPEE469 pKa = 3.76 NFLGNHH475 pKa = 5.81 YY476 pKa = 10.32 EE477 pKa = 4.43 DD478 pKa = 5.09 DD479 pKa = 4.03 AATEE483 pKa = 4.83 AILGQASEE491 pKa = 4.35 ADD493 pKa = 3.57 EE494 pKa = 4.39 AARR497 pKa = 11.84 TALIEE502 pKa = 4.18 DD503 pKa = 3.77 AQRR506 pKa = 11.84 IVAEE510 pKa = 4.58 DD511 pKa = 4.54 LSTLPYY517 pKa = 10.46 LQGAQVAVAGTDD529 pKa = 3.21 ITGVTLDD536 pKa = 3.42 ASFKK540 pKa = 10.46 FRR542 pKa = 11.84 YY543 pKa = 9.76 APLGRR548 pKa = 4.77
Molecular weight: 58.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.783
IPC_protein 3.795
Toseland 3.592
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.49
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.101
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.923
Patrickios 1.43
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.799
Protein with the highest isoelectric point:
>tr|A0A1I2HW18|A0A1I2HW18_9MICO Ribosomal protein S18 acetylase RimI OS=Flavimobilis marinus OX=285351 GN=SAMN04488035_2546 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.16 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MSTRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.65 GRR40 pKa = 11.84 SEE42 pKa = 3.94 LSAA45 pKa = 4.73
Molecular weight: 5.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.438
IPC2_protein 10.935
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.31
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.047
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.117
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2643
0
2643
894391
33
2433
338.4
35.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.351 ± 0.074
0.56 ± 0.011
6.319 ± 0.039
5.647 ± 0.045
2.63 ± 0.03
9.062 ± 0.043
2.07 ± 0.024
3.417 ± 0.034
1.8 ± 0.036
10.208 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.749 ± 0.019
1.622 ± 0.026
5.628 ± 0.038
2.714 ± 0.023
7.589 ± 0.048
5.144 ± 0.031
6.324 ± 0.044
9.855 ± 0.054
1.445 ± 0.018
1.868 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here