Tepidibacter thalassicus DSM 15285

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Tepidibacter; Tepidibacter thalassicus

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2250 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5PIW2|A0A1M5PIW2_9FIRM Methyltransferase domain-containing protein OS=Tepidibacter thalassicus DSM 15285 OX=1123350 GN=SAMN02744040_00517 PE=4 SV=1
MM1 pKa = 7.95AFQQLTATVTTSADD15 pKa = 3.3NQAVVTLNSGILDD28 pKa = 3.56KK29 pKa = 11.36GYY31 pKa = 10.89VYY33 pKa = 10.86YY34 pKa = 10.66FIAAINADD42 pKa = 3.0GTTYY46 pKa = 10.66YY47 pKa = 11.33GEE49 pKa = 4.28TFKK52 pKa = 11.34EE53 pKa = 4.04DD54 pKa = 3.43LKK56 pKa = 10.59TATEE60 pKa = 4.35DD61 pKa = 4.09KK62 pKa = 10.75DD63 pKa = 4.49LSVNNIVLPNIPISSDD79 pKa = 3.25FTGTLTNEE87 pKa = 3.67NVTLYY92 pKa = 10.92VSYY95 pKa = 11.11TEE97 pKa = 4.11

Molecular weight: 10.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5RXM0|A0A1M5RXM0_9FIRM MazG-like family protein OS=Tepidibacter thalassicus DSM 15285 OX=1123350 GN=SAMN02744040_01560 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.0KK9 pKa = 7.87RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.77RR21 pKa = 11.84MKK23 pKa = 8.92TKK25 pKa = 10.17SGRR28 pKa = 11.84NILKK32 pKa = 9.88RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.15RR41 pKa = 11.84LTAA44 pKa = 4.25

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2250

0

2250

670041

41

2849

297.8

33.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.019 ± 0.053

1.07 ± 0.024

5.542 ± 0.051

7.686 ± 0.067

4.379 ± 0.039

6.218 ± 0.049

1.282 ± 0.02

10.725 ± 0.065

10.239 ± 0.057

8.939 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.499 ± 0.024

6.605 ± 0.052

2.729 ± 0.03

2.164 ± 0.023

3.378 ± 0.039

5.759 ± 0.041

4.386 ± 0.038

6.582 ± 0.046

0.593 ± 0.017

4.206 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski