Mangrovimonas yunxiaonensis
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2307 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A084TJU7|A0A084TJU7_9FLAO Uncharacterized protein OS=Mangrovimonas yunxiaonensis OX=1197477 GN=IA57_11140 PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.3 NSNYY6 pKa = 9.9 SRR8 pKa = 11.84 LVSIFITLLTLVTSCSSDD26 pKa = 4.08 DD27 pKa = 4.74 DD28 pKa = 4.06 NTSNDD33 pKa = 3.89 PNNSSTFLDD42 pKa = 3.51 KK43 pKa = 10.0 WWYY46 pKa = 10.56 DD47 pKa = 3.31 SDD49 pKa = 5.55 DD50 pKa = 3.86 FAADD54 pKa = 3.39 IYY56 pKa = 10.68 FHH58 pKa = 7.37 SNGEE62 pKa = 4.09 YY63 pKa = 8.51 EE64 pKa = 4.23 QKK66 pKa = 10.7 KK67 pKa = 8.73 VVQGTSYY74 pKa = 10.3 IANGDD79 pKa = 3.15 WVYY82 pKa = 11.07 EE83 pKa = 4.13 NEE85 pKa = 4.03 SSGIIRR91 pKa = 11.84 IDD93 pKa = 3.38 NLVGNGQTQSTIWLKK108 pKa = 10.85 VSNIQVNTITIQQSSDD124 pKa = 2.92 GTDD127 pKa = 3.14 YY128 pKa = 11.53 SEE130 pKa = 5.44 AMDD133 pKa = 4.75 YY134 pKa = 11.17 QDD136 pKa = 3.81 TDD138 pKa = 3.08 NN139 pKa = 4.62
Molecular weight: 15.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.554
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.77
Rodwell 3.605
Grimsley 3.465
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.19
Thurlkill 3.617
EMBOSS 3.77
Sillero 3.91
Patrickios 0.947
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A084TID8|A0A084TID8_9FLAO 50S ribosomal protein L29 OS=Mangrovimonas yunxiaonensis OX=1197477 GN=rpmC PE=3 SV=1
MM1 pKa = 7.32 SVKK4 pKa = 9.96 KK5 pKa = 10.34 LKK7 pKa = 10.43 PITPGQRR14 pKa = 11.84 FRR16 pKa = 11.84 VVNGYY21 pKa = 9.53 DD22 pKa = 4.32 AITTDD27 pKa = 3.72 KK28 pKa = 11.01 PEE30 pKa = 4.39 KK31 pKa = 10.55 SLLAPKK37 pKa = 9.91 KK38 pKa = 10.22 RR39 pKa = 11.84 SGGRR43 pKa = 11.84 NSQGKK48 pKa = 6.48 MTMRR52 pKa = 11.84 YY53 pKa = 9.3 IGGGHH58 pKa = 6.61 KK59 pKa = 9.72 KK60 pKa = 9.77 RR61 pKa = 11.84 YY62 pKa = 9.44 RR63 pKa = 11.84 IIDD66 pKa = 3.78 FKK68 pKa = 11.1 RR69 pKa = 11.84 NKK71 pKa = 9.94 AGVSAEE77 pKa = 4.05 VMSIEE82 pKa = 3.98 YY83 pKa = 10.47 DD84 pKa = 3.48 PNRR87 pKa = 11.84 TAFIALLKK95 pKa = 10.95 YY96 pKa = 10.31 EE97 pKa = 4.98 DD98 pKa = 3.76 GEE100 pKa = 4.02 KK101 pKa = 10.3 RR102 pKa = 11.84 YY103 pKa = 10.22 IIAQNGLQVGQNVISGQEE121 pKa = 3.89 NVPTEE126 pKa = 4.12 IGNTMPLSKK135 pKa = 10.29 IPLGTIISCVEE146 pKa = 3.63 LRR148 pKa = 11.84 PGQGAVMARR157 pKa = 11.84 SAGAFAQLMARR168 pKa = 11.84 DD169 pKa = 4.26 GKK171 pKa = 10.62 FATIKK176 pKa = 10.5 LPSGEE181 pKa = 4.11 TRR183 pKa = 11.84 LVLVSCMATIGVVSNSDD200 pKa = 3.21 HH201 pKa = 6.09 QLLVSGKK208 pKa = 9.49 AGRR211 pKa = 11.84 SRR213 pKa = 11.84 WLGRR217 pKa = 11.84 RR218 pKa = 11.84 PRR220 pKa = 11.84 TRR222 pKa = 11.84 PVVMNPVDD230 pKa = 3.47 HH231 pKa = 7.05 PMGGGEE237 pKa = 3.89 GRR239 pKa = 11.84 ASGGHH244 pKa = 5.06 PRR246 pKa = 11.84 SRR248 pKa = 11.84 KK249 pKa = 8.85 GLPAKK254 pKa = 10.34 GYY256 pKa = 7.1 RR257 pKa = 11.84 TRR259 pKa = 11.84 SKK261 pKa = 9.56 TKK263 pKa = 10.12 ASNKK267 pKa = 9.84 YY268 pKa = 8.91 IVEE271 pKa = 4.08 RR272 pKa = 11.84 RR273 pKa = 11.84 KK274 pKa = 10.24 KK275 pKa = 10.1
Molecular weight: 30.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.926
IPC_protein 10.745
Toseland 11.023
ProMoST 10.76
Dawson 11.082
Bjellqvist 10.789
Wikipedia 11.301
Rodwell 11.374
Grimsley 11.125
Solomon 11.242
Lehninger 11.199
Nozaki 10.994
DTASelect 10.789
Thurlkill 11.008
EMBOSS 11.433
Sillero 11.023
Patrickios 11.082
IPC_peptide 11.242
IPC2_peptide 9.677
IPC2.peptide.svr19 8.462
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2307
0
2307
774474
47
2785
335.7
37.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.16 ± 0.052
0.801 ± 0.025
5.531 ± 0.056
6.269 ± 0.055
5.071 ± 0.043
6.323 ± 0.054
2.041 ± 0.028
7.391 ± 0.048
7.577 ± 0.076
9.449 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.213 ± 0.028
6.055 ± 0.053
3.473 ± 0.03
3.652 ± 0.031
3.313 ± 0.039
5.922 ± 0.047
6.2 ± 0.065
6.566 ± 0.04
0.969 ± 0.018
4.026 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here