Plasmodium gonderi
Average proteome isoelectric point is 7.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5915 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y1JIA4|A0A1Y1JIA4_PLAGO Non-specific serine/threonine protein kinase OS=Plasmodium gonderi OX=77519 GN=PGO_091160 PE=3 SV=1
MM1 pKa = 7.57 EE2 pKa = 5.66 EE3 pKa = 4.12 GMSNNEE9 pKa = 3.94 SSNGVLDD16 pKa = 4.23 EE17 pKa = 4.59 EE18 pKa = 4.54 SQEE21 pKa = 4.16 DD22 pKa = 4.2 EE23 pKa = 4.68 RR24 pKa = 11.84 DD25 pKa = 3.28 LSYY28 pKa = 10.65 STFCNQEE35 pKa = 3.57 EE36 pKa = 4.5 GRR38 pKa = 11.84 DD39 pKa = 3.58 LSYY42 pKa = 11.62 DD43 pKa = 3.48 SFCNQEE49 pKa = 3.58 EE50 pKa = 4.23 RR51 pKa = 11.84 GNISDD56 pKa = 3.46 NSSYY60 pKa = 10.88 NQEE63 pKa = 4.27 EE64 pKa = 4.36 EE65 pKa = 4.16 IKK67 pKa = 10.06 CQKK70 pKa = 10.8 NEE72 pKa = 4.08 DD73 pKa = 3.69 HH74 pKa = 7.46 DD75 pKa = 4.26 GCANAGNYY83 pKa = 10.06 LDD85 pKa = 5.46 DD86 pKa = 4.67 CNEE89 pKa = 3.9 PKK91 pKa = 10.86 GDD93 pKa = 4.03 VRR95 pKa = 11.84 DD96 pKa = 4.27 KK97 pKa = 11.62 EE98 pKa = 3.96 NDD100 pKa = 3.14 ADD102 pKa = 3.91 GGQNEE107 pKa = 4.31 EE108 pKa = 4.27 EE109 pKa = 4.23 EE110 pKa = 4.39 EE111 pKa = 4.19 EE112 pKa = 4.24 EE113 pKa = 4.16 EE114 pKa = 4.21 EE115 pKa = 4.21 EE116 pKa = 4.21 EE117 pKa = 4.21 EE118 pKa = 4.21 EE119 pKa = 4.21 EE120 pKa = 4.21 EE121 pKa = 4.21 EE122 pKa = 4.21 EE123 pKa = 4.21 EE124 pKa = 4.21 EE125 pKa = 4.21 EE126 pKa = 4.21 EE127 pKa = 4.32 EE128 pKa = 4.49 EE129 pKa = 5.03 EE130 pKa = 5.02 EE131 pKa = 5.58 EE132 pKa = 5.35 EE133 pKa = 6.18 DD134 pKa = 6.18 DD135 pKa = 6.61 DD136 pKa = 7.5 DD137 pKa = 7.66 DD138 pKa = 7.7 DD139 pKa = 7.67 DD140 pKa = 7.69 DD141 pKa = 7.37 DD142 pKa = 7.09 DD143 pKa = 7.33 DD144 pKa = 6.58 YY145 pKa = 12.12 DD146 pKa = 5.19 DD147 pKa = 6.26 DD148 pKa = 6.19 EE149 pKa = 6.21 YY150 pKa = 11.78 DD151 pKa = 3.47 EE152 pKa = 6.54 DD153 pKa = 4.75 DD154 pKa = 4.12 FNEE157 pKa = 4.25 ATVSWIEE164 pKa = 3.85 WFCQLKK170 pKa = 10.52 QNVFLVEE177 pKa = 3.73 VDD179 pKa = 3.01 EE180 pKa = 5.6 DD181 pKa = 4.57 FIRR184 pKa = 11.84 DD185 pKa = 3.7 EE186 pKa = 4.51 FNLIGLQTKK195 pKa = 9.4 VPHH198 pKa = 6.15 FKK200 pKa = 10.65 KK201 pKa = 10.34 LLKK204 pKa = 10.33 IILDD208 pKa = 4.07 EE209 pKa = 6.84 DD210 pKa = 5.47 DD211 pKa = 6.35 DD212 pKa = 7.41 DD213 pKa = 7.56 DD214 pKa = 7.67 DD215 pKa = 7.63 DD216 pKa = 7.14 DD217 pKa = 6.94 DD218 pKa = 6.04 EE219 pKa = 6.22 EE220 pKa = 6.6 DD221 pKa = 6.07 DD222 pKa = 4.29 YY223 pKa = 12.15 DD224 pKa = 5.36 EE225 pKa = 6.96 DD226 pKa = 5.22 DD227 pKa = 5.26 DD228 pKa = 4.99 EE229 pKa = 5.65 INRR232 pKa = 11.84 GSDD235 pKa = 3.19 EE236 pKa = 4.29 LYY238 pKa = 10.5 KK239 pKa = 11.25 NKK241 pKa = 10.36 DD242 pKa = 2.95 IYY244 pKa = 9.74 EE245 pKa = 4.04 QNAACLYY252 pKa = 10.34 GLIHH256 pKa = 6.74 SRR258 pKa = 11.84 FILTSKK264 pKa = 10.6 GLALMRR270 pKa = 11.84 EE271 pKa = 4.53 KK272 pKa = 10.68 YY273 pKa = 10.08 KK274 pKa = 11.0 SSIYY278 pKa = 8.53 GTCPSIYY285 pKa = 9.91 CDD287 pKa = 3.46 NAKK290 pKa = 10.36 LLPTAISEE298 pKa = 4.45 VPKK301 pKa = 10.54 FLSPLLYY308 pKa = 10.36 CPRR311 pKa = 11.84 CCEE314 pKa = 3.42 TYY316 pKa = 10.3 YY317 pKa = 10.09 PHH319 pKa = 7.58 KK320 pKa = 10.84 NSLLNEE326 pKa = 4.14 LDD328 pKa = 2.99 GSYY331 pKa = 10.55 FGTSFASFFALSFNIQSDD349 pKa = 3.52 KK350 pKa = 11.0 KK351 pKa = 10.21 RR352 pKa = 11.84 IYY354 pKa = 8.27 YY355 pKa = 8.27 TPQIFGFTINRR366 pKa = 11.84 DD367 pKa = 2.7 IRR369 pKa = 11.84 EE370 pKa = 4.0 NLFLDD375 pKa = 3.58 MNKK378 pKa = 10.24 EE379 pKa = 3.98 NTEE382 pKa = 4.01 SLEE385 pKa = 4.25 EE386 pKa = 4.12 YY387 pKa = 10.73 SS388 pKa = 4.14
Molecular weight: 45.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.807
IPC2_protein 3.732
IPC_protein 3.745
Toseland 3.528
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.605
Rodwell 3.567
Grimsley 3.439
Solomon 3.706
Lehninger 3.668
Nozaki 3.821
DTASelect 4.012
Thurlkill 3.567
EMBOSS 3.617
Sillero 3.859
Patrickios 2.193
IPC_peptide 3.706
IPC2_peptide 3.834
IPC2.peptide.svr19 3.777
Protein with the highest isoelectric point:
>tr|A0A1Y1JIP1|A0A1Y1JIP1_PLAGO TBC domain protein OS=Plasmodium gonderi OX=77519 GN=PGO_120820 PE=4 SV=1
MM1 pKa = 7.28 AHH3 pKa = 6.31 GASRR7 pKa = 11.84 YY8 pKa = 8.75 KK9 pKa = 10.48 KK10 pKa = 10.3 SRR12 pKa = 11.84 AKK14 pKa = 9.88 MRR16 pKa = 11.84 WKK18 pKa = 9.16 WKK20 pKa = 9.29 KK21 pKa = 9.72 KK22 pKa = 6.67 RR23 pKa = 11.84 TRR25 pKa = 11.84 RR26 pKa = 11.84 LQKK29 pKa = 9.69 KK30 pKa = 7.67 RR31 pKa = 11.84 RR32 pKa = 11.84 KK33 pKa = 8.24 MRR35 pKa = 11.84 QRR37 pKa = 11.84 SRR39 pKa = 3.22
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.436
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.676
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.486
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.193
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.032
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5915
0
5915
4048994
36
11537
684.5
79.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.865 ± 0.02
2.006 ± 0.015
5.666 ± 0.022
7.838 ± 0.045
4.634 ± 0.028
4.007 ± 0.029
2.499 ± 0.012
7.934 ± 0.036
10.839 ± 0.037
7.895 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.34 ± 0.014
10.377 ± 0.054
2.308 ± 0.017
2.904 ± 0.014
3.761 ± 0.023
7.703 ± 0.027
4.523 ± 0.016
4.469 ± 0.016
0.579 ± 0.007
4.852 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here