Macacine gammaherpesvirus 4 (Rhesus lymphocryptovirus)
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8UZC8|Q8UZC8_9GAMA BNLF2a OS=Macacine gammaherpesvirus 4 OX=45455 PE=4 SV=1
MM1 pKa = 7.49 EE2 pKa = 5.08 GNRR5 pKa = 11.84 GRR7 pKa = 11.84 GGGHH11 pKa = 5.58 RR12 pKa = 11.84 HH13 pKa = 5.63 PRR15 pKa = 11.84 CPQPHH20 pKa = 6.79 ASLLFGSLLLLVAILVWFFIIMSDD44 pKa = 3.82 LTQTASTVLSSFAVVLIIIIIIIMLFKK71 pKa = 10.67 RR72 pKa = 11.84 RR73 pKa = 11.84 LLCPLGLLCIILIMIVILVSNLLTLTGQTLFVGLVILCLCILLALAVWFYY123 pKa = 11.4 LMWLLRR129 pKa = 11.84 EE130 pKa = 4.12 YY131 pKa = 10.72 GASFWTILAFCLAFLLAIVFLIIAVLLHH159 pKa = 6.18 LAWFTVLVDD168 pKa = 5.16 LYY170 pKa = 10.68 WLLLFLAILIWIYY183 pKa = 11.02 VHH185 pKa = 7.04 DD186 pKa = 4.89 FNRR189 pKa = 11.84 AIPEE193 pKa = 3.88 ALANLEE199 pKa = 4.13 EE200 pKa = 4.73 EE201 pKa = 4.88 YY202 pKa = 11.04 NHH204 pKa = 6.37 QGHH207 pKa = 5.88 GHH209 pKa = 6.58 GGGQGDD215 pKa = 3.61 EE216 pKa = 4.19 HH217 pKa = 7.07 QPRR220 pKa = 11.84 VLLISEE226 pKa = 4.35 DD227 pKa = 3.85 PKK229 pKa = 9.55 THH231 pKa = 6.08 HH232 pKa = 6.64 HH233 pKa = 6.22 HH234 pKa = 6.49 HH235 pKa = 6.59 HH236 pKa = 6.16 HH237 pKa = 6.2 HH238 pKa = 5.78 HH239 pKa = 6.83 HH240 pKa = 6.51 KK241 pKa = 10.64 NSNQGDD247 pKa = 3.81 PLGPYY252 pKa = 9.87 VSQNGGNGGNEE263 pKa = 3.69 GDD265 pKa = 3.78 GGNRR269 pKa = 11.84 DD270 pKa = 3.63 GGNGDD275 pKa = 3.98 GGNGDD280 pKa = 4.1 GGNVGDD286 pKa = 4.76 GGNGNGGNGDD296 pKa = 3.9 GGNGDD301 pKa = 4.23 GGNGDD306 pKa = 4.62 GGNEE310 pKa = 4.43 GPDD313 pKa = 3.37 HH314 pKa = 6.93 LPYY317 pKa = 10.35 PIQATDD323 pKa = 3.26 GGNGGEE329 pKa = 3.95 GDD331 pKa = 4.35 GGNEE335 pKa = 4.32 GPDD338 pKa = 3.61 PLPYY342 pKa = 10.11 PIQATDD348 pKa = 3.62 GGNGGQGDD356 pKa = 4.21 GGDD359 pKa = 3.7 GDD361 pKa = 4.54 GGNGGNGGGGGDD373 pKa = 4.57 PDD375 pKa = 4.75 HH376 pKa = 7.06 LPHH379 pKa = 7.6 PIQATDD385 pKa = 3.53 GGNSGDD391 pKa = 4.38 GGDD394 pKa = 4.51 GGDD397 pKa = 4.2 GEE399 pKa = 5.03 DD400 pKa = 4.6 GEE402 pKa = 5.17 GGGDD406 pKa = 3.22 RR407 pKa = 11.84 GPFGPYY413 pKa = 9.56 VSQGPSGDD421 pKa = 4.24 PDD423 pKa = 4.0 HH424 pKa = 7.07 LPHH427 pKa = 7.16 PVQASDD433 pKa = 3.76 GGDD436 pKa = 3.14 GLGPYY441 pKa = 9.93 GPAGPFTQGPSWPWGPFGTGPLGPWGPFGPGPCGPWGPWGPNNNHH486 pKa = 6.42 GPLQEE491 pKa = 4.41 TGPGGPWMLLTLGGGGNSVHH511 pKa = 6.94 LNDD514 pKa = 5.28 RR515 pKa = 11.84 GNGGNGPQNPDD526 pKa = 2.93 NSNGSGPGGPHH537 pKa = 5.25 NQQPEE542 pKa = 4.44 SNEE545 pKa = 3.96 GGGPPGPTDD554 pKa = 3.78 GGNNGPDD561 pKa = 3.71 SPPSNQGPQGPEE573 pKa = 3.81 GDD575 pKa = 4.47 PGDD578 pKa = 4.28 PADD581 pKa = 5.39 PIQISYY587 pKa = 11.0 YY588 pKa = 10.57 DD589 pKa = 3.28
Molecular weight: 60.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.157
IPC2_protein 4.355
IPC_protein 4.38
Toseland 4.215
ProMoST 4.507
Dawson 4.38
Bjellqvist 4.533
Wikipedia 4.317
Rodwell 4.228
Grimsley 4.113
Solomon 4.38
Lehninger 4.342
Nozaki 4.495
DTASelect 4.749
Thurlkill 4.24
EMBOSS 4.329
Sillero 4.533
Patrickios 1.151
IPC_peptide 4.38
IPC2_peptide 4.507
IPC2.peptide.svr19 4.371
Protein with the highest isoelectric point:
>tr|Q8UZE2|Q8UZE2_9GAMA BILF2 OS=Macacine gammaherpesvirus 4 OX=45455 PE=4 SV=1
MM1 pKa = 7.48 GLGFRR6 pKa = 11.84 GWGLHH11 pKa = 6.25 EE12 pKa = 4.45 PACLPGEE19 pKa = 4.31 PNQRR23 pKa = 11.84 TQGHH27 pKa = 6.82 PAPGPSSRR35 pKa = 11.84 TGQRR39 pKa = 11.84 TRR41 pKa = 11.84 QRR43 pKa = 11.84 PGHH46 pKa = 6.35 PPPEE50 pKa = 4.87 RR51 pKa = 11.84 GSGPRR56 pKa = 11.84 GTRR59 pKa = 11.84 PPAPSSRR66 pKa = 11.84 TGQRR70 pKa = 11.84 TRR72 pKa = 11.84 QRR74 pKa = 11.84 PGHH77 pKa = 6.35 PPPEE81 pKa = 4.87 RR82 pKa = 11.84 GSGPRR87 pKa = 11.84 GTRR90 pKa = 11.84 PPAPSSRR97 pKa = 11.84 TGQRR101 pKa = 11.84 TRR103 pKa = 11.84 QRR105 pKa = 11.84 PGHH108 pKa = 6.35 PPPEE112 pKa = 4.87 RR113 pKa = 11.84 GSGPRR118 pKa = 11.84 GTRR121 pKa = 11.84 PPAPSSRR128 pKa = 11.84 TGQRR132 pKa = 11.84 TRR134 pKa = 11.84 QRR136 pKa = 11.84 PGHH139 pKa = 6.35 PPPEE143 pKa = 4.87 RR144 pKa = 11.84 GSGPRR149 pKa = 11.84 GTRR152 pKa = 11.84 PPAPSSRR159 pKa = 11.84 TGQRR163 pKa = 11.84 TRR165 pKa = 11.84 QRR167 pKa = 11.84 PGHH170 pKa = 6.35 PPPEE174 pKa = 4.87 RR175 pKa = 11.84 GSGPRR180 pKa = 11.84 GTRR183 pKa = 11.84 PPAPSSRR190 pKa = 11.84 TGQRR194 pKa = 11.84 TRR196 pKa = 11.84 QRR198 pKa = 11.84 PGHH201 pKa = 6.35 PPPEE205 pKa = 4.87 RR206 pKa = 11.84 GSGPRR211 pKa = 11.84 GTRR214 pKa = 11.84 PPAPSSRR221 pKa = 11.84 TGQRR225 pKa = 11.84 TRR227 pKa = 11.84 QRR229 pKa = 11.84 PGHH232 pKa = 6.35 PPPEE236 pKa = 4.87 RR237 pKa = 11.84 GSGPRR242 pKa = 11.84 GTRR245 pKa = 11.84 PPAPSSRR252 pKa = 11.84 TGQRR256 pKa = 11.84 TRR258 pKa = 11.84 QRR260 pKa = 11.84 PGHH263 pKa = 6.35 PPPEE267 pKa = 4.87 RR268 pKa = 11.84 GSGPRR273 pKa = 11.84 GTRR276 pKa = 11.84 PPAPSSRR283 pKa = 11.84 TGQRR287 pKa = 11.84 TRR289 pKa = 11.84 QRR291 pKa = 11.84 PGHH294 pKa = 6.35 PPPEE298 pKa = 4.87 RR299 pKa = 11.84 GSGPRR304 pKa = 11.84 GTRR307 pKa = 11.84 PPAPSSRR314 pKa = 11.84 TGQRR318 pKa = 11.84 TRR320 pKa = 11.84 QRR322 pKa = 11.84 PGHH325 pKa = 6.35 PPPEE329 pKa = 4.87 RR330 pKa = 11.84 GSGPRR335 pKa = 11.84 GTRR338 pKa = 11.84 PPAPSSRR345 pKa = 11.84 TGQRR349 pKa = 11.84 TRR351 pKa = 11.84 QRR353 pKa = 11.84 PGHH356 pKa = 6.35 PPPEE360 pKa = 4.87 RR361 pKa = 11.84 GSGPRR366 pKa = 11.84 GTRR369 pKa = 11.84 PPAPSSRR376 pKa = 11.84 TGQRR380 pKa = 11.84 TRR382 pKa = 11.84 QRR384 pKa = 11.84 PGHH387 pKa = 6.35 PPPEE391 pKa = 4.87 RR392 pKa = 11.84 GSGPRR397 pKa = 11.84 GTRR400 pKa = 11.84 PPAPSSRR407 pKa = 11.84 TGQRR411 pKa = 11.84 TRR413 pKa = 11.84 QRR415 pKa = 11.84 PGHH418 pKa = 6.35 PPPEE422 pKa = 4.87 RR423 pKa = 11.84 GSGPRR428 pKa = 11.84 GTRR431 pKa = 11.84 PPAPSSRR438 pKa = 11.84 TGQRR442 pKa = 11.84 TRR444 pKa = 11.84 QRR446 pKa = 11.84 PGHH449 pKa = 6.35 PPPEE453 pKa = 4.87 RR454 pKa = 11.84 GSGPRR459 pKa = 11.84 GTRR462 pKa = 11.84 PPAPSSRR469 pKa = 11.84 TGQRR473 pKa = 11.84 TRR475 pKa = 11.84 QRR477 pKa = 11.84 PGHH480 pKa = 6.35 PPPEE484 pKa = 4.87 RR485 pKa = 11.84 GSGPRR490 pKa = 11.84 GTRR493 pKa = 11.84 PPAPSSRR500 pKa = 11.84 TGQRR504 pKa = 11.84 TRR506 pKa = 11.84 QRR508 pKa = 11.84 PGHH511 pKa = 6.35 PPPEE515 pKa = 4.87 RR516 pKa = 11.84 GSGPRR521 pKa = 11.84 GTRR524 pKa = 11.84 PPAPSSRR531 pKa = 11.84 TGQRR535 pKa = 11.84 TRR537 pKa = 11.84 QRR539 pKa = 11.84 PGHH542 pKa = 6.35 PPPEE546 pKa = 4.87 RR547 pKa = 11.84 GSGPRR552 pKa = 11.84 GTRR555 pKa = 11.84 PPAPSSRR562 pKa = 11.84 TGQRR566 pKa = 11.84 TRR568 pKa = 11.84 QRR570 pKa = 11.84 PGHH573 pKa = 6.35 PPPEE577 pKa = 4.87 RR578 pKa = 11.84 GSGPRR583 pKa = 11.84 GTRR586 pKa = 11.84 PPAPSSRR593 pKa = 11.84 TGQRR597 pKa = 11.84 TRR599 pKa = 11.84 QRR601 pKa = 11.84 PGHH604 pKa = 6.35 PPPEE608 pKa = 4.87 RR609 pKa = 11.84 GSGPRR614 pKa = 11.84 GTRR617 pKa = 11.84 PPAPSSRR624 pKa = 11.84 TGQRR628 pKa = 11.84 TRR630 pKa = 11.84 QRR632 pKa = 11.84 PGHH635 pKa = 6.35 PPPEE639 pKa = 4.87 RR640 pKa = 11.84 GSGPRR645 pKa = 11.84 GTRR648 pKa = 11.84 PPAPSSRR655 pKa = 11.84 TGQRR659 pKa = 11.84 TRR661 pKa = 11.84 QRR663 pKa = 11.84 PGHH666 pKa = 6.35 PPPEE670 pKa = 4.87 RR671 pKa = 11.84 GSGPRR676 pKa = 11.84 GTRR679 pKa = 11.84 PPAPSSRR686 pKa = 11.84 TGQRR690 pKa = 11.84 TRR692 pKa = 11.84 QRR694 pKa = 11.84 PGHH697 pKa = 6.35 PPPEE701 pKa = 4.87 RR702 pKa = 11.84 GSGPRR707 pKa = 11.84 GTRR710 pKa = 11.84 PPAPSSRR717 pKa = 11.84 TGQRR721 pKa = 11.84 TRR723 pKa = 11.84 QRR725 pKa = 11.84 PGHH728 pKa = 6.35 PPPEE732 pKa = 4.87 RR733 pKa = 11.84 GSGPRR738 pKa = 11.84 GTRR741 pKa = 11.84 PPAPSSRR748 pKa = 11.84 TGQRR752 pKa = 11.84 TRR754 pKa = 11.84 QRR756 pKa = 11.84 PGHH759 pKa = 6.35 PPPEE763 pKa = 4.87 RR764 pKa = 11.84 GSGPRR769 pKa = 11.84 GTRR772 pKa = 11.84 PPAPSSRR779 pKa = 11.84 TGQRR783 pKa = 11.84 TRR785 pKa = 11.84 QRR787 pKa = 11.84 PGHH790 pKa = 6.35 PPPEE794 pKa = 4.87 RR795 pKa = 11.84 GSGPRR800 pKa = 11.84 GTRR803 pKa = 11.84 PPAPSSRR810 pKa = 11.84 TGQRR814 pKa = 11.84 TRR816 pKa = 11.84 QRR818 pKa = 11.84 PGHH821 pKa = 6.35 PPPEE825 pKa = 4.87 RR826 pKa = 11.84 GSGPRR831 pKa = 11.84 GTRR834 pKa = 11.84 PPAPSSRR841 pKa = 11.84 TGQRR845 pKa = 11.84 TRR847 pKa = 11.84 QRR849 pKa = 11.84 PGHH852 pKa = 6.51 PPPEE856 pKa = 4.08 QTQTLGKK863 pKa = 10.12 RR864 pKa = 11.84 GARR867 pKa = 11.84 VFNNKK872 pKa = 8.0 VYY874 pKa = 10.86 KK875 pKa = 9.38 LTSRR879 pKa = 11.84 VCGACGVGVVGG890 pKa = 5.45
Molecular weight: 95.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.179
Grimsley 12.705
Solomon 13.159
Lehninger 13.071
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 11.871
IPC_peptide 13.173
IPC2_peptide 12.164
IPC2.peptide.svr19 9.166
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
80
0
80
40829
59
3105
510.4
55.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.452 ± 0.461
1.876 ± 0.164
4.257 ± 0.162
5.643 ± 0.208
3.644 ± 0.206
7.781 ± 0.46
2.459 ± 0.115
3.331 ± 0.202
2.959 ± 0.191
10.216 ± 0.361
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.842 ± 0.116
2.851 ± 0.224
8.758 ± 0.64
3.975 ± 0.202
7.196 ± 0.382
7.272 ± 0.18
6.03 ± 0.262
6.486 ± 0.233
1.203 ± 0.082
2.77 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here