bacterium D16-63

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1845 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9AVH4|A0A3A9AVH4_9BACT ATP-binding protein OS=bacterium D16-63 OX=2320119 GN=D7X10_08945 PE=4 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84APVVSRR8 pKa = 11.84TSKK11 pKa = 10.66LSAVLLVLLLACTLGPAAMADD32 pKa = 3.81EE33 pKa = 4.97PSADD37 pKa = 3.6SAVGGVGADD46 pKa = 3.21AADD49 pKa = 3.89AEE51 pKa = 4.71GAPMLASLMASGAGHH66 pKa = 7.43ISTAVALEE74 pKa = 4.59DD75 pKa = 4.03DD76 pKa = 4.58PEE78 pKa = 4.29PVVGSFTVGGLTYY91 pKa = 10.43AIVSEE96 pKa = 4.69GEE98 pKa = 4.21VVLVSVGPDD107 pKa = 2.9ADD109 pKa = 3.73ALAVGSNGAGEE120 pKa = 4.33GLLANSSGAPSGEE133 pKa = 4.14DD134 pKa = 2.91SGSGVPPRR142 pKa = 11.84SVAEE146 pKa = 4.0QVPSGAASLSPSPEE160 pKa = 3.81GVPSDD165 pKa = 3.58GAEE168 pKa = 4.38GEE170 pKa = 4.51DD171 pKa = 3.65ADD173 pKa = 4.72DD174 pKa = 4.18PVVLEE179 pKa = 4.26VPEE182 pKa = 4.24SVEE185 pKa = 4.03FDD187 pKa = 3.08GVAYY191 pKa = 10.17SVVAIGPRR199 pKa = 11.84ALAGCDD205 pKa = 2.87ADD207 pKa = 5.43AIVIPTTVGSVDD219 pKa = 3.16EE220 pKa = 4.19LAFRR224 pKa = 11.84GSAVKK229 pKa = 10.45AIEE232 pKa = 4.11VAGGNASFSSFDD244 pKa = 3.17GMLFDD249 pKa = 5.53AEE251 pKa = 4.69RR252 pKa = 11.84ISLLLIPEE260 pKa = 5.1GKK262 pKa = 9.87QGAARR267 pKa = 11.84IPSTAEE273 pKa = 3.49AVPPGAFSHH282 pKa = 6.3CASVTSLSVDD292 pKa = 3.26AGSAAYY298 pKa = 10.44LSRR301 pKa = 11.84NGCLYY306 pKa = 10.56DD307 pKa = 3.66ASGEE311 pKa = 4.01ALLRR315 pKa = 11.84VPAGATEE322 pKa = 3.89IVIADD327 pKa = 3.93GCTAVAAGALEE338 pKa = 4.62GCAKK342 pKa = 10.16LRR344 pKa = 11.84AIQASASVTEE354 pKa = 4.02VSPDD358 pKa = 3.36VLGAAVAADD367 pKa = 3.89GGSAPVASLGSSGALAADD385 pKa = 4.98GGSQMGDD392 pKa = 2.97VGDD395 pKa = 4.34ADD397 pKa = 3.73AAEE400 pKa = 5.03GGSADD405 pKa = 4.07GPQAPFGDD413 pKa = 4.08EE414 pKa = 4.15GRR416 pKa = 11.84GLDD419 pKa = 4.47AEE421 pKa = 4.6AEE423 pKa = 4.29APAGSSPAVSSQLTSLVVLATAGDD447 pKa = 4.43DD448 pKa = 3.8LPQVNSTSIIVSLPEE463 pKa = 4.27GADD466 pKa = 3.48PAPWEE471 pKa = 3.93AVGFEE476 pKa = 4.41VEE478 pKa = 5.07VIAADD483 pKa = 3.5ADD485 pKa = 4.19GNAEE489 pKa = 4.17TVEE492 pKa = 4.28GEE494 pKa = 4.32SLDD497 pKa = 5.54DD498 pKa = 3.53ITPEE502 pKa = 4.21DD503 pKa = 3.79AHH505 pKa = 7.81DD506 pKa = 4.47HH507 pKa = 7.08DD508 pKa = 5.35VDD510 pKa = 4.25EE511 pKa = 5.75PDD513 pKa = 4.84DD514 pKa = 4.52APSDD518 pKa = 3.88GPVIVDD524 pKa = 4.19EE525 pKa = 4.23VTIDD529 pKa = 3.24YY530 pKa = 10.48GYY532 pKa = 11.4ANEE535 pKa = 4.49RR536 pKa = 11.84SSADD540 pKa = 3.12GAYY543 pKa = 9.59EE544 pKa = 3.82RR545 pKa = 11.84AVPKK549 pKa = 10.16IDD551 pKa = 4.9AVLKK555 pKa = 8.68LQPITAEE562 pKa = 3.73EE563 pKa = 4.09LMEE566 pKa = 4.5GEE568 pKa = 4.23VAARR572 pKa = 11.84GEE574 pKa = 4.16DD575 pKa = 3.42TSAGFGSAADD585 pKa = 4.23GSADD589 pKa = 3.86GACGAVDD596 pKa = 3.7SDD598 pKa = 3.8AGSGGGAAADD608 pKa = 3.86AADD611 pKa = 4.23ADD613 pKa = 4.21EE614 pKa = 5.33GEE616 pKa = 4.35PTLASKK622 pKa = 10.64LADD625 pKa = 3.77LPEE628 pKa = 5.11DD629 pKa = 3.83SQATASFLYY638 pKa = 9.9EE639 pKa = 3.82GCLYY643 pKa = 10.99VIDD646 pKa = 4.71PEE648 pKa = 5.38GGAALVAVDD657 pKa = 4.37PKK659 pKa = 10.87RR660 pKa = 11.84LAEE663 pKa = 4.19CVEE666 pKa = 4.6DD667 pKa = 3.93PTTLILPDD675 pKa = 3.89YY676 pKa = 10.42VDD678 pKa = 4.86DD679 pKa = 4.68GTWSYY684 pKa = 11.5PLTRR688 pKa = 11.84IAKK691 pKa = 8.7GALRR695 pKa = 11.84GSGIEE700 pKa = 4.11RR701 pKa = 11.84LWIPASATYY710 pKa = 10.67LDD712 pKa = 4.29YY713 pKa = 11.35ALEE716 pKa = 4.51GCEE719 pKa = 3.57TLRR722 pKa = 11.84YY723 pKa = 10.06VEE725 pKa = 4.45ISEE728 pKa = 5.15DD729 pKa = 3.58SPAYY733 pKa = 9.88SSKK736 pKa = 10.92GGCLYY741 pKa = 11.0DD742 pKa = 4.01KK743 pKa = 10.91AGEE746 pKa = 4.2KK747 pKa = 10.38LWMVPEE753 pKa = 4.65GGGDD757 pKa = 3.19IAIEE761 pKa = 4.07VAVDD765 pKa = 3.75GGAGEE770 pKa = 4.51INPARR775 pKa = 11.84QTDD778 pKa = 3.72QAKK781 pKa = 8.66LTNIAQMHH789 pKa = 5.67YY790 pKa = 9.61PASLSNLSTARR801 pKa = 11.84APRR804 pKa = 11.84TSYY807 pKa = 9.44RR808 pKa = 11.84TTHH811 pKa = 7.06LYY813 pKa = 6.35TTNFHH818 pKa = 6.74SVTDD822 pKa = 3.68YY823 pKa = 7.64TTQVDD828 pKa = 3.43VDD830 pKa = 4.16GTCVSGCEE838 pKa = 4.09TDD840 pKa = 4.08DD841 pKa = 5.04FPWTLIWTLRR851 pKa = 11.84RR852 pKa = 11.84QITNDD857 pKa = 2.91RR858 pKa = 11.84GIYY861 pKa = 9.97FYY863 pKa = 10.88TDD865 pKa = 3.22GTISEE870 pKa = 4.35MDD872 pKa = 3.57PYY874 pKa = 11.17GQLDD878 pKa = 3.77YY879 pKa = 11.26QYY881 pKa = 10.8VNTISSSRR889 pKa = 11.84IYY891 pKa = 9.39RR892 pKa = 11.84THH894 pKa = 6.4LAAWSSSPLGPPLTHH909 pKa = 6.73SLHH912 pKa = 6.18TGPIYY917 pKa = 9.6LHH919 pKa = 6.82CGVGYY924 pKa = 10.57LKK926 pKa = 9.33QTVTWADD933 pKa = 3.33TATGSTQTEE942 pKa = 4.24TVPLNGATTAPSPSRR957 pKa = 11.84TGYY960 pKa = 8.41TFAGWYY966 pKa = 5.89TAASGGSYY974 pKa = 10.43VCGAGGSVTVTSDD987 pKa = 3.0VTYY990 pKa = 9.81YY991 pKa = 11.28AHH993 pKa = 6.65WTPNTYY999 pKa = 8.66TVSS1002 pKa = 3.29

Molecular weight: 101.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9B2R6|A0A3A9B2R6_9BACT Alpha/beta hydrolase OS=bacterium D16-63 OX=2320119 GN=D7X10_07525 PE=4 SV=1
GG1 pKa = 7.38RR2 pKa = 11.84GGPGGRR8 pKa = 11.84GGPGAGRR15 pKa = 11.84GAGGRR20 pKa = 11.84GAAHH24 pKa = 6.17QGGGQGPGRR33 pKa = 11.84GVRR36 pKa = 11.84HH37 pKa = 5.91RR38 pKa = 11.84AQRR41 pKa = 11.84GLL43 pKa = 3.46

Molecular weight: 3.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1845

0

1845

639091

27

2417

346.4

37.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.361 ± 0.083

1.411 ± 0.027

5.943 ± 0.048

6.612 ± 0.063

3.432 ± 0.038

9.161 ± 0.061

1.666 ± 0.022

4.075 ± 0.051

3.136 ± 0.047

9.228 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.44 ± 0.034

2.337 ± 0.035

4.963 ± 0.041

2.891 ± 0.025

6.115 ± 0.062

5.871 ± 0.07

5.2 ± 0.068

8.002 ± 0.053

1.267 ± 0.034

2.891 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski