Amino acid dipepetide frequency for bacterium D16-63

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.065AlaAla: 19.065 ± 0.327	2.051AlaCys: 2.051 ± 0.074	7.689AlaAsp: 7.689 ± 0.125	8.789AlaGlu: 8.789 ± 0.142	4.968AlaPhe: 4.968 ± 0.1	11.192AlaGly: 11.192 ± 0.177	2.144AlaHis: 2.144 ± 0.061	5.744AlaIle: 5.744 ± 0.099	4.212AlaLys: 4.212 ± 0.093	13.762AlaLeu: 13.762 ± 0.236	3.521AlaMet: 3.521 ± 0.086	2.943AlaAsn: 2.943 ± 0.092	6.415AlaPro: 6.415 ± 0.136	4.272AlaGln: 4.272 ± 0.11	7.677AlaArg: 7.677 ± 0.126	8.165AlaSer: 8.165 ± 0.165	6.556AlaThr: 6.556 ± 0.124	9.715AlaVal: 9.715 ± 0.15	1.465AlaTrp: 1.465 ± 0.06	3.262AlaTyr: 3.262 ± 0.071	0.0AlaXaa: 0.0 ± 0.0
Cys	1.968CysAla: 1.968 ± 0.058	0.244CysCys: 0.244 ± 0.022	0.934CysAsp: 0.934 ± 0.043	0.909CysGlu: 0.909 ± 0.037	0.476CysPhe: 0.476 ± 0.026	1.476CysGly: 1.476 ± 0.049	0.271CysHis: 0.271 ± 0.022	0.602CysIle: 0.602 ± 0.035	0.407CysLys: 0.407 ± 0.024	1.271CysLeu: 1.271 ± 0.045	0.28CysMet: 0.28 ± 0.019	0.283CysAsn: 0.283 ± 0.023	0.762CysPro: 0.762 ± 0.04	0.341CysGln: 0.341 ± 0.022	0.715CysArg: 0.715 ± 0.038	0.729CysSer: 0.729 ± 0.034	0.76CysThr: 0.76 ± 0.033	1.109CysVal: 1.109 ± 0.047	0.177CysTrp: 0.177 ± 0.017	0.393CysTyr: 0.393 ± 0.027	0.0CysXaa: 0.0 ± 0.0
Asp	8.576AspAla: 8.576 ± 0.139	0.648AspCys: 0.648 ± 0.034	3.605AspAsp: 3.605 ± 0.094	4.858AspGlu: 4.858 ± 0.093	1.907AspPhe: 1.907 ± 0.054	6.911AspGly: 6.911 ± 0.157	0.887AspHis: 0.887 ± 0.037	2.399AspIle: 2.399 ± 0.058	1.738AspLys: 1.738 ± 0.057	5.243AspLeu: 5.243 ± 0.097	1.235AspMet: 1.235 ± 0.048	1.106AspAsn: 1.106 ± 0.049	3.414AspPro: 3.414 ± 0.088	1.554AspGln: 1.554 ± 0.055	3.075AspArg: 3.075 ± 0.078	2.704AspSer: 2.704 ± 0.07	2.187AspThr: 2.187 ± 0.065	4.808AspVal: 4.808 ± 0.099	0.688AspTrp: 0.688 ± 0.038	1.879AspTyr: 1.879 ± 0.058	0.0AspXaa: 0.0 ± 0.0
Glu	9.709GluAla: 9.709 ± 0.175	0.71GluCys: 0.71 ± 0.035	3.403GluAsp: 3.403 ± 0.083	4.71GluGlu: 4.71 ± 0.116	1.685GluPhe: 1.685 ± 0.061	7.675GluGly: 7.675 ± 0.14	1.216GluHis: 1.216 ± 0.047	2.004GluIle: 2.004 ± 0.071	2.142GluLys: 2.142 ± 0.069	6.149GluLeu: 6.149 ± 0.102	1.447GluMet: 1.447 ± 0.053	1.322GluAsn: 1.322 ± 0.051	3.264GluPro: 3.264 ± 0.082	2.167GluGln: 2.167 ± 0.066	5.531GluArg: 5.531 ± 0.112	2.888GluSer: 2.888 ± 0.07	2.46GluThr: 2.46 ± 0.062	5.555GluVal: 5.555 ± 0.11	0.577GluTrp: 0.577 ± 0.031	1.502GluTyr: 1.502 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	4.398PheAla: 4.398 ± 0.095	0.552PheCys: 0.552 ± 0.035	2.95PheAsp: 2.95 ± 0.069	2.452PheGlu: 2.452 ± 0.065	1.127PhePhe: 1.127 ± 0.054	3.289PheGly: 3.289 ± 0.076	0.499PheHis: 0.499 ± 0.03	1.317PheIle: 1.317 ± 0.053	1.044PheLys: 1.044 ± 0.043	2.884PheLeu: 2.884 ± 0.087	0.784PheMet: 0.784 ± 0.032	0.969PheAsn: 0.969 ± 0.04	1.328PhePro: 1.328 ± 0.053	0.898PheGln: 0.898 ± 0.036	1.626PheArg: 1.626 ± 0.053	2.208PheSer: 2.208 ± 0.06	1.939PheThr: 1.939 ± 0.061	2.669PheVal: 2.669 ± 0.078	0.416PheTrp: 0.416 ± 0.027	0.97PheTyr: 0.97 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	11.563GlyAla: 11.563 ± 0.193	1.815GlyCys: 1.815 ± 0.073	4.736GlyAsp: 4.736 ± 0.098	5.481GlyGlu: 5.481 ± 0.116	3.375GlyPhe: 3.375 ± 0.076	7.969GlyGly: 7.969 ± 0.168	1.573GlyHis: 1.573 ± 0.057	4.183GlyIle: 4.183 ± 0.1	3.14GlyLys: 3.14 ± 0.091	8.041GlyLeu: 8.041 ± 0.122	2.366GlyMet: 2.366 ± 0.063	2.203GlyAsn: 2.203 ± 0.065	3.322GlyPro: 3.322 ± 0.084	2.868GlyGln: 2.868 ± 0.054	5.353GlyArg: 5.353 ± 0.116	6.756GlySer: 6.756 ± 0.173	5.336GlyThr: 5.336 ± 0.126	6.44GlyVal: 6.44 ± 0.118	1.831GlyTrp: 1.831 ± 0.105	3.253GlyTyr: 3.253 ± 0.099	0.0GlyXaa: 0.0 ± 0.0
His	1.806HisAla: 1.806 ± 0.052	0.341HisCys: 0.341 ± 0.026	1.092HisAsp: 1.092 ± 0.037	1.056HisGlu: 1.056 ± 0.046	0.513HisPhe: 0.513 ± 0.028	1.901HisGly: 1.901 ± 0.06	0.344HisHis: 0.344 ± 0.026	0.626HisIle: 0.626 ± 0.031	0.444HisLys: 0.444 ± 0.027	1.488HisLeu: 1.488 ± 0.056	0.319HisMet: 0.319 ± 0.025	0.413HisAsn: 0.413 ± 0.023	1.033HisPro: 1.033 ± 0.043	0.419HisGln: 0.419 ± 0.026	1.078HisArg: 1.078 ± 0.038	0.904HisSer: 0.904 ± 0.041	0.706HisThr: 0.706 ± 0.036	1.333HisVal: 1.333 ± 0.048	0.246HisTrp: 0.246 ± 0.025	0.601HisTyr: 0.601 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.405IleAla: 5.405 ± 0.128	0.548IleCys: 0.548 ± 0.032	3.09IleAsp: 3.09 ± 0.078	3.248IleGlu: 3.248 ± 0.082	1.117IlePhe: 1.117 ± 0.051	3.652IleGly: 3.652 ± 0.087	0.656IleHis: 0.656 ± 0.035	1.781IleIle: 1.781 ± 0.067	1.325IleLys: 1.325 ± 0.047	2.945IleLeu: 2.945 ± 0.076	1.056IleMet: 1.056 ± 0.047	1.102IleAsn: 1.102 ± 0.046	2.258IlePro: 2.258 ± 0.059	1.07IleGln: 1.07 ± 0.044	1.914IleArg: 1.914 ± 0.065	2.145IleSer: 2.145 ± 0.068	2.331IleThr: 2.331 ± 0.073	3.708IleVal: 3.708 ± 0.081	0.396IleTrp: 0.396 ± 0.028	1.008IleTyr: 1.008 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	4.054LysAla: 4.054 ± 0.098	0.307LysCys: 0.307 ± 0.023	1.737LysAsp: 1.737 ± 0.056	1.87LysGlu: 1.87 ± 0.066	0.648LysPhe: 0.648 ± 0.036	2.953LysGly: 2.953 ± 0.073	0.563LysHis: 0.563 ± 0.033	1.025LysIle: 1.025 ± 0.044	1.468LysLys: 1.468 ± 0.06	2.887LysLeu: 2.887 ± 0.075	0.753LysMet: 0.753 ± 0.036	0.836LysAsn: 0.836 ± 0.041	1.726LysPro: 1.726 ± 0.06	1.07LysGln: 1.07 ± 0.045	2.447LysArg: 2.447 ± 0.065	1.516LysSer: 1.516 ± 0.053	1.645LysThr: 1.645 ± 0.057	2.751LysVal: 2.751 ± 0.084	0.357LysTrp: 0.357 ± 0.03	0.746LysTyr: 0.746 ± 0.04	0.0LysXaa: 0.0 ± 0.0
Leu	13.147LeuAla: 13.147 ± 0.221	1.35LeuCys: 1.35 ± 0.051	5.697LeuAsp: 5.697 ± 0.088	5.597LeuGlu: 5.597 ± 0.119	3.3LeuPhe: 3.3 ± 0.085	8.025LeuGly: 8.025 ± 0.143	1.394LeuHis: 1.394 ± 0.052	3.219LeuIle: 3.219 ± 0.09	2.685LeuLys: 2.685 ± 0.081	8.274LeuLeu: 8.274 ± 0.177	2.455LeuMet: 2.455 ± 0.064	1.953LeuAsn: 1.953 ± 0.058	4.843LeuPro: 4.843 ± 0.102	2.277LeuGln: 2.277 ± 0.059	6.034LeuArg: 6.034 ± 0.125	5.689LeuSer: 5.689 ± 0.102	4.819LeuThr: 4.819 ± 0.086	7.871LeuVal: 7.871 ± 0.129	1.089LeuTrp: 1.089 ± 0.043	2.558LeuTyr: 2.558 ± 0.063	0.0LeuXaa: 0.0 ± 0.0
Met	3.585MetAla: 3.585 ± 0.096	0.211MetCys: 0.211 ± 0.019	1.302MetAsp: 1.302 ± 0.051	1.641MetGlu: 1.641 ± 0.056	0.642MetPhe: 0.642 ± 0.036	2.2MetGly: 2.2 ± 0.067	0.39MetHis: 0.39 ± 0.026	0.801MetIle: 0.801 ± 0.035	0.804MetLys: 0.804 ± 0.038	2.363MetLeu: 2.363 ± 0.057	0.659MetMet: 0.659 ± 0.037	0.637MetAsn: 0.637 ± 0.032	1.471MetPro: 1.471 ± 0.051	0.671MetGln: 0.671 ± 0.034	1.748MetArg: 1.748 ± 0.056	1.36MetSer: 1.36 ± 0.056	1.46MetThr: 1.46 ± 0.052	1.848MetVal: 1.848 ± 0.065	0.16MetTrp: 0.16 ± 0.016	0.451MetTyr: 0.451 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.782AsnAla: 2.782 ± 0.075	0.286AsnCys: 0.286 ± 0.024	1.335AsnAsp: 1.335 ± 0.055	1.292AsnGlu: 1.292 ± 0.051	0.701AsnPhe: 0.701 ± 0.034	2.436AsnGly: 2.436 ± 0.097	0.449AsnHis: 0.449 ± 0.03	1.059AsnIle: 1.059 ± 0.047	0.695AsnLys: 0.695 ± 0.038	2.219AsnLeu: 2.219 ± 0.063	0.56AsnMet: 0.56 ± 0.033	0.648AsnAsn: 0.648 ± 0.05	1.729AsnPro: 1.729 ± 0.071	0.696AsnGln: 0.696 ± 0.037	1.353AsnArg: 1.353 ± 0.053	1.03AsnSer: 1.03 ± 0.042	1.167AsnThr: 1.167 ± 0.052	1.943AsnVal: 1.943 ± 0.062	0.319AsnTrp: 0.319 ± 0.025	0.667AsnTyr: 0.667 ± 0.039	0.0AsnXaa: 0.0 ± 0.0
Pro	6.397ProAla: 6.397 ± 0.142	0.643ProCys: 0.643 ± 0.034	3.216ProAsp: 3.216 ± 0.096	4.165ProGlu: 4.165 ± 0.084	1.684ProPhe: 1.684 ± 0.054	4.15ProGly: 4.15 ± 0.098	0.836ProHis: 0.836 ± 0.033	1.921ProIle: 1.921 ± 0.059	1.588ProLys: 1.588 ± 0.05	4.356ProLeu: 4.356 ± 0.095	1.094ProMet: 1.094 ± 0.043	1.231ProAsn: 1.231 ± 0.063	1.831ProPro: 1.831 ± 0.059	1.394ProGln: 1.394 ± 0.047	2.474ProArg: 2.474 ± 0.066	3.519ProSer: 3.519 ± 0.107	3.003ProThr: 3.003 ± 0.079	4.053ProVal: 4.053 ± 0.071	0.679ProTrp: 0.679 ± 0.028	1.4ProTyr: 1.4 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	4.334GlnAla: 4.334 ± 0.104	0.321GlnCys: 0.321 ± 0.022	1.321GlnAsp: 1.321 ± 0.046	1.712GlnGlu: 1.712 ± 0.065	0.762GlnPhe: 0.762 ± 0.033	2.878GlnGly: 2.878 ± 0.063	0.499GlnHis: 0.499 ± 0.026	1.111GlnIle: 1.111 ± 0.046	0.918GlnLys: 0.918 ± 0.036	2.56GlnLeu: 2.56 ± 0.07	0.754GlnMet: 0.754 ± 0.032	0.624GlnAsn: 0.624 ± 0.035	1.554GlnPro: 1.554 ± 0.059	1.192GlnGln: 1.192 ± 0.046	2.131GlnArg: 2.131 ± 0.065	1.319GlnSer: 1.319 ± 0.048	1.261GlnThr: 1.261 ± 0.049	2.576GlnVal: 2.576 ± 0.064	0.448GlnTrp: 0.448 ± 0.027	0.632GlnTyr: 0.632 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	7.45ArgAla: 7.45 ± 0.127	0.912ArgCys: 0.912 ± 0.046	3.22ArgAsp: 3.22 ± 0.079	4.15ArgGlu: 4.15 ± 0.086	2.702ArgPhe: 2.702 ± 0.063	4.253ArgGly: 4.253 ± 0.088	1.208ArgHis: 1.208 ± 0.043	3.153ArgIle: 3.153 ± 0.075	2.037ArgLys: 2.037 ± 0.066	5.733ArgLeu: 5.733 ± 0.114	1.657ArgMet: 1.657 ± 0.049	1.496ArgAsn: 1.496 ± 0.047	2.948ArgPro: 2.948 ± 0.074	1.921ArgGln: 1.921 ± 0.06	4.458ArgArg: 4.458 ± 0.112	3.577ArgSer: 3.577 ± 0.087	3.162ArgThr: 3.162 ± 0.075	4.12ArgVal: 4.12 ± 0.084	0.973ArgTrp: 0.973 ± 0.043	2.018ArgTyr: 2.018 ± 0.055	0.0ArgXaa: 0.0 ± 0.0
Ser	7.088SerAla: 7.088 ± 0.136	0.798SerCys: 0.798 ± 0.041	3.137SerAsp: 3.137 ± 0.075	2.957SerGlu: 2.957 ± 0.085	2.643SerPhe: 2.643 ± 0.068	5.489SerGly: 5.489 ± 0.127	1.083SerHis: 1.083 ± 0.042	2.723SerIle: 2.723 ± 0.072	1.821SerLys: 1.821 ± 0.061	5.61SerLeu: 5.61 ± 0.119	1.615SerMet: 1.615 ± 0.051	1.349SerAsn: 1.349 ± 0.048	2.896SerPro: 2.896 ± 0.082	1.688SerGln: 1.688 ± 0.051	3.173SerArg: 3.173 ± 0.077	4.079SerSer: 4.079 ± 0.12	3.04SerThr: 3.04 ± 0.088	4.531SerVal: 4.531 ± 0.113	0.814SerTrp: 0.814 ± 0.043	2.173SerTyr: 2.173 ± 0.072	0.0SerXaa: 0.0 ± 0.0
Thr	6.215ThrAla: 6.215 ± 0.115	0.721ThrCys: 0.721 ± 0.036	2.676ThrAsp: 2.676 ± 0.084	2.652ThrGlu: 2.652 ± 0.071	2.106ThrPhe: 2.106 ± 0.076	5.013ThrGly: 5.013 ± 0.122	0.801ThrHis: 0.801 ± 0.035	2.466ThrIle: 2.466 ± 0.073	1.424ThrLys: 1.424 ± 0.056	5.284ThrLeu: 5.284 ± 0.101	1.127ThrMet: 1.127 ± 0.042	1.163ThrAsn: 1.163 ± 0.051	2.798ThrPro: 2.798 ± 0.076	1.244ThrGln: 1.244 ± 0.045	2.676ThrArg: 2.676 ± 0.072	3.122ThrSer: 3.122 ± 0.105	3.007ThrThr: 3.007 ± 0.1	4.708ThrVal: 4.708 ± 0.109	0.751ThrTrp: 0.751 ± 0.044	2.047ThrTyr: 2.047 ± 0.086	0.0ThrXaa: 0.0 ± 0.0
Val	11.0ValAla: 11.0 ± 0.174	1.089ValCys: 1.089 ± 0.043	5.002ValAsp: 5.002 ± 0.092	5.75ValGlu: 5.75 ± 0.099	2.515ValPhe: 2.515 ± 0.071	6.484ValGly: 6.484 ± 0.105	1.133ValHis: 1.133 ± 0.042	3.264ValIle: 3.264 ± 0.092	2.244ValLys: 2.244 ± 0.066	7.267ValLeu: 7.267 ± 0.141	1.864ValMet: 1.864 ± 0.059	1.906ValAsn: 1.906 ± 0.065	4.449ValPro: 4.449 ± 0.1	1.945ValGln: 1.945 ± 0.069	4.995ValArg: 4.995 ± 0.079	4.647ValSer: 4.647 ± 0.09	4.71ValThr: 4.71 ± 0.119	7.146ValVal: 7.146 ± 0.123	0.756ValTrp: 0.756 ± 0.04	1.854ValTyr: 1.854 ± 0.056	0.0ValXaa: 0.0 ± 0.0
Trp	1.471TrpAla: 1.471 ± 0.065	0.169TrpCys: 0.169 ± 0.016	0.851TrpAsp: 0.851 ± 0.047	0.92TrpGlu: 0.92 ± 0.043	0.48TrpPhe: 0.48 ± 0.03	0.948TrpGly: 0.948 ± 0.045	0.235TrpHis: 0.235 ± 0.019	0.376TrpIle: 0.376 ± 0.024	0.435TrpLys: 0.435 ± 0.03	1.197TrpLeu: 1.197 ± 0.049	0.316TrpMet: 0.316 ± 0.021	0.393TrpAsn: 0.393 ± 0.027	0.466TrpPro: 0.466 ± 0.032	0.415TrpGln: 0.415 ± 0.031	0.89TrpArg: 0.89 ± 0.045	0.803TrpSer: 0.803 ± 0.047	0.732TrpThr: 0.732 ± 0.058	0.928TrpVal: 0.928 ± 0.046	0.217TrpTrp: 0.217 ± 0.019	0.426TrpTyr: 0.426 ± 0.035	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.594TyrAla: 3.594 ± 0.107	0.39TyrCys: 0.39 ± 0.028	2.432TyrAsp: 2.432 ± 0.081	1.907TyrGlu: 1.907 ± 0.061	0.969TyrPhe: 0.969 ± 0.04	2.71TyrGly: 2.71 ± 0.067	0.485TyrHis: 0.485 ± 0.028	0.981TyrIle: 0.981 ± 0.041	0.784TyrLys: 0.784 ± 0.041	2.745TyrLeu: 2.745 ± 0.073	0.54TyrMet: 0.54 ± 0.027	0.792TyrAsn: 0.792 ± 0.042	1.127TyrPro: 1.127 ± 0.042	0.803TyrGln: 0.803 ± 0.039	1.804TyrArg: 1.804 ± 0.05	1.548TyrSer: 1.548 ± 0.063	1.718TyrThr: 1.718 ± 0.095	2.205TyrVal: 2.205 ± 0.056	0.31TyrTrp: 0.31 ± 0.023	1.069TyrTyr: 1.069 ± 0.056	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1845 proteins (639091 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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