Streptococcus phage Javan235

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A729|A0A4D6A729_9CAUD Uncharacterized protein OS=Streptococcus phage Javan235 OX=2548060 GN=Javan235_0038 PE=4 SV=1
MM1 pKa = 7.89ADD3 pKa = 3.31TEE5 pKa = 4.23RR6 pKa = 11.84CEE8 pKa = 4.95GCGCDD13 pKa = 4.44FRR15 pKa = 11.84EE16 pKa = 4.23GTIDD20 pKa = 3.71YY21 pKa = 10.68DD22 pKa = 3.88CVFANGYY29 pKa = 9.25CCNCLANEE37 pKa = 4.28EE38 pKa = 4.51DD39 pKa = 4.72DD40 pKa = 3.82NN41 pKa = 4.8

Molecular weight: 4.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A4C8|A0A4D6A4C8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan235 OX=2548060 GN=Javan235_0054 PE=4 SV=1
MM1 pKa = 7.37SIKK4 pKa = 10.65YY5 pKa = 9.46SVKK8 pKa = 10.54GVDD11 pKa = 2.51RR12 pKa = 11.84WTKK15 pKa = 10.21RR16 pKa = 11.84LRR18 pKa = 11.84DD19 pKa = 3.68KK20 pKa = 11.23SKK22 pKa = 9.74QAQVATDD29 pKa = 3.62RR30 pKa = 11.84QLEE33 pKa = 4.17LSSKK37 pKa = 9.93RR38 pKa = 11.84IEE40 pKa = 4.27RR41 pKa = 11.84GAKK44 pKa = 8.52TGAPVDD50 pKa = 3.58TGALKK55 pKa = 9.19NTIFSVKK62 pKa = 10.06AGHH65 pKa = 6.52LTYY68 pKa = 10.77KK69 pKa = 9.03VTAPQHH75 pKa = 4.35YY76 pKa = 9.9AIYY79 pKa = 10.09VEE81 pKa = 4.0KK82 pKa = 9.85GTRR85 pKa = 11.84KK86 pKa = 8.65MRR88 pKa = 11.84AQPFLKK94 pKa = 10.19PAIDD98 pKa = 3.83AEE100 pKa = 4.34QPKK103 pKa = 10.49LISNLRR109 pKa = 11.84KK110 pKa = 9.88LYY112 pKa = 10.4EE113 pKa = 3.86RR114 pKa = 4.77

Molecular weight: 12.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13671

39

1658

231.7

25.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.068 ± 0.769

0.607 ± 0.136

6.261 ± 0.45

6.92 ± 0.633

3.84 ± 0.196

6.064 ± 0.416

1.426 ± 0.154

6.496 ± 0.276

7.915 ± 0.43

8.346 ± 0.349

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.194

6.093 ± 0.322

2.641 ± 0.249

4.645 ± 0.279

3.592 ± 0.408

6.656 ± 0.708

7.051 ± 0.537

6.122 ± 0.214

0.987 ± 0.133

3.928 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski