Halobacterium sp. PCN9

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Halobacterium; unclassified Halobacterium

Average proteome isoelectric point is 4.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3549 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B0SZ70|A0A6B0SZ70_9EURY DUF3179 domain-containing protein OS=Halobacterium sp. PCN9 OX=2692200 GN=GRX66_19290 PE=4 SV=1
MM1 pKa = 7.74SDD3 pKa = 3.1YY4 pKa = 11.19DD5 pKa = 4.07AYY7 pKa = 11.31LAGDD11 pKa = 3.84RR12 pKa = 11.84TDD14 pKa = 4.79HH15 pKa = 5.69VALYY19 pKa = 10.45LSEE22 pKa = 5.45SYY24 pKa = 11.36VSDD27 pKa = 3.26VDD29 pKa = 3.85TLADD33 pKa = 3.76RR34 pKa = 11.84EE35 pKa = 4.23DD36 pKa = 3.8AEE38 pKa = 4.38RR39 pKa = 11.84VADD42 pKa = 4.14GVVLVVDD49 pKa = 4.29GEE51 pKa = 4.26RR52 pKa = 11.84GRR54 pKa = 11.84SVFQTITGMSAMDD67 pKa = 4.2FGSAAMDD74 pKa = 3.41NPGRR78 pKa = 11.84IDD80 pKa = 3.76ADD82 pKa = 4.31LVDD85 pKa = 4.6GDD87 pKa = 4.62CPEE90 pKa = 4.66AANEE94 pKa = 4.26SADD97 pKa = 3.46GADD100 pKa = 3.39DD101 pKa = 3.77HH102 pKa = 7.58RR103 pKa = 11.84PEE105 pKa = 4.31FVFSFAEE112 pKa = 4.09AQNEE116 pKa = 4.35DD117 pKa = 3.62VGGLYY122 pKa = 10.52ADD124 pKa = 3.99GDD126 pKa = 4.21VIHH129 pKa = 7.22AYY131 pKa = 9.98VYY133 pKa = 9.46CSCGTAYY140 pKa = 10.49SDD142 pKa = 2.83KK143 pKa = 10.25WVAGEE148 pKa = 3.95RR149 pKa = 3.77

Molecular weight: 16.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B0SKG8|A0A6B0SKG8_9EURY Glutamine synthetase OS=Halobacterium sp. PCN9 OX=2692200 GN=glnA PE=3 SV=1
MM1 pKa = 7.83PGFPDD6 pKa = 2.97ARR8 pKa = 11.84HH9 pKa = 5.61AVRR12 pKa = 11.84IAVNEE17 pKa = 3.88LRR19 pKa = 11.84VGWRR23 pKa = 11.84KK24 pKa = 9.43VRR26 pKa = 11.84GKK28 pKa = 9.2STVQLLAAAAAILFGLVFTAAAAWGAHH55 pKa = 5.27IAGATLAEE63 pKa = 4.51NPDD66 pKa = 3.82DD67 pKa = 4.27AATLAGLVPRR77 pKa = 11.84RR78 pKa = 11.84VRR80 pKa = 11.84RR81 pKa = 11.84STCSWPAYY89 pKa = 8.54LTAIQLGDD97 pKa = 2.89IDD99 pKa = 5.46ARR101 pKa = 11.84DD102 pKa = 4.09GYY104 pKa = 9.94LTTIPARR111 pKa = 11.84DD112 pKa = 3.66VVGGLLLAGYY122 pKa = 9.36VRR124 pKa = 11.84VVGYY128 pKa = 8.17FAAPLVVAAAAFAVGAGAPLAFPARR153 pKa = 11.84DD154 pKa = 3.39AGRR157 pKa = 11.84VRR159 pKa = 11.84AHH161 pKa = 7.03RR162 pKa = 11.84DD163 pKa = 3.1GVLVLL168 pKa = 4.39

Molecular weight: 17.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3549

0

3549

923842

33

2372

260.3

28.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.188 ± 0.061

0.687 ± 0.013

8.939 ± 0.051

8.1 ± 0.054

3.431 ± 0.031

8.444 ± 0.039

2.087 ± 0.021

3.472 ± 0.035

1.743 ± 0.025

8.721 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.722 ± 0.019

2.395 ± 0.027

4.612 ± 0.029

2.606 ± 0.026

6.315 ± 0.043

5.46 ± 0.031

6.367 ± 0.047

9.808 ± 0.046

1.14 ± 0.017

2.764 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski