Amino acid dipepetide frequency for Halobacterium sp. PCN9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.26AlaAla: 14.26 ± 0.178
0.748AlaCys: 0.748 ± 0.031
10.831AlaAsp: 10.831 ± 0.125
8.111AlaGlu: 8.111 ± 0.12
4.265AlaPhe: 4.265 ± 0.074
9.939AlaGly: 9.939 ± 0.139
1.948AlaHis: 1.948 ± 0.049
4.322AlaIle: 4.322 ± 0.081
1.837AlaLys: 1.837 ± 0.052
10.18AlaLeu: 10.18 ± 0.131
2.133AlaMet: 2.133 ± 0.05
2.666AlaAsn: 2.666 ± 0.06
4.082AlaPro: 4.082 ± 0.07
2.195AlaGln: 2.195 ± 0.052
5.934AlaArg: 5.934 ± 0.087
6.031AlaSer: 6.031 ± 0.092
6.892AlaThr: 6.892 ± 0.102
11.428AlaVal: 11.428 ± 0.164
1.253AlaTrp: 1.253 ± 0.039
2.823AlaTyr: 2.823 ± 0.056
0.0AlaXaa: 0.0 ± 0.0
Cys
0.588CysAla: 0.588 ± 0.023
0.088CysCys: 0.088 ± 0.011
0.524CysAsp: 0.524 ± 0.027
0.591CysGlu: 0.591 ± 0.027
0.186CysPhe: 0.186 ± 0.014
0.861CysGly: 0.861 ± 0.034
0.185CysHis: 0.185 ± 0.017
0.259CysIle: 0.259 ± 0.016
0.101CysLys: 0.101 ± 0.01
0.473CysLeu: 0.473 ± 0.025
0.105CysMet: 0.105 ± 0.011
0.175CysAsn: 0.175 ± 0.014
0.504CysPro: 0.504 ± 0.025
0.17CysGln: 0.17 ± 0.014
0.447CysArg: 0.447 ± 0.023
0.443CysSer: 0.443 ± 0.023
0.395CysThr: 0.395 ± 0.023
0.507CysVal: 0.507 ± 0.023
0.089CysTrp: 0.089 ± 0.011
0.176CysTyr: 0.176 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
11.692AspAla: 11.692 ± 0.149
0.704AspCys: 0.704 ± 0.03
8.525AspAsp: 8.525 ± 0.122
7.556AspGlu: 7.556 ± 0.104
2.255AspPhe: 2.255 ± 0.054
8.336AspGly: 8.336 ± 0.127
1.986AspHis: 1.986 ± 0.049
2.857AspIle: 2.857 ± 0.065
1.043AspLys: 1.043 ± 0.035
7.3AspLeu: 7.3 ± 0.108
1.304AspMet: 1.304 ± 0.039
1.424AspAsn: 1.424 ± 0.048
4.859AspPro: 4.859 ± 0.077
1.912AspGln: 1.912 ± 0.05
5.929AspArg: 5.929 ± 0.092
3.929AspSer: 3.929 ± 0.074
4.436AspThr: 4.436 ± 0.087
9.887AspVal: 9.887 ± 0.112
1.176AspTrp: 1.176 ± 0.038
2.275AspTyr: 2.275 ± 0.061
0.0AspXaa: 0.0 ± 0.0
Glu
8.151GluAla: 8.151 ± 0.131
0.52GluCys: 0.52 ± 0.03
6.675GluAsp: 6.675 ± 0.118
5.7GluGlu: 5.7 ± 0.113
3.243GluPhe: 3.243 ± 0.065
4.975GluGly: 4.975 ± 0.083
2.126GluHis: 2.126 ± 0.048
2.999GluIle: 2.999 ± 0.061
1.636GluLys: 1.636 ± 0.055
7.161GluLeu: 7.161 ± 0.106
1.81GluMet: 1.81 ± 0.043
2.317GluAsn: 2.317 ± 0.064
3.515GluPro: 3.515 ± 0.076
2.82GluGln: 2.82 ± 0.066
6.92GluArg: 6.92 ± 0.109
4.754GluSer: 4.754 ± 0.085
6.054GluThr: 6.054 ± 0.096
5.765GluVal: 5.765 ± 0.083
1.076GluTrp: 1.076 ± 0.036
2.784GluTyr: 2.784 ± 0.056
0.0GluXaa: 0.0 ± 0.0
Phe
3.894PheAla: 3.894 ± 0.082
0.25PheCys: 0.25 ± 0.019
3.471PheAsp: 3.471 ± 0.066
3.219PheGlu: 3.219 ± 0.061
1.011PhePhe: 1.011 ± 0.039
3.279PheGly: 3.279 ± 0.072
0.671PheHis: 0.671 ± 0.026
0.948PheIle: 0.948 ± 0.038
0.461PheLys: 0.461 ± 0.023
2.913PheLeu: 2.913 ± 0.066
0.501PheMet: 0.501 ± 0.023
0.74PheAsn: 0.74 ± 0.028
1.39PhePro: 1.39 ± 0.039
0.892PheGln: 0.892 ± 0.03
1.875PheArg: 1.875 ± 0.047
1.765PheSer: 1.765 ± 0.048
1.999PheThr: 1.999 ± 0.048
3.728PheVal: 3.728 ± 0.074
0.369PheTrp: 0.369 ± 0.023
0.93PheTyr: 0.93 ± 0.033
0.0PheXaa: 0.0 ± 0.0
Gly
8.246GlyAla: 8.246 ± 0.114
0.711GlyCys: 0.711 ± 0.03
7.73GlyAsp: 7.73 ± 0.114
6.813GlyGlu: 6.813 ± 0.098
3.334GlyPhe: 3.334 ± 0.067
7.409GlyGly: 7.409 ± 0.124
1.678GlyHis: 1.678 ± 0.044
3.621GlyIle: 3.621 ± 0.083
1.696GlyLys: 1.696 ± 0.046
6.915GlyLeu: 6.915 ± 0.109
1.668GlyMet: 1.668 ± 0.049
1.972GlyAsn: 1.972 ± 0.055
3.233GlyPro: 3.233 ± 0.058
2.073GlyGln: 2.073 ± 0.049
4.64GlyArg: 4.64 ± 0.076
4.901GlySer: 4.901 ± 0.09
5.467GlyThr: 5.467 ± 0.081
8.63GlyVal: 8.63 ± 0.104
1.038GlyTrp: 1.038 ± 0.031
2.666GlyTyr: 2.666 ± 0.055
0.0GlyXaa: 0.0 ± 0.0
His
2.2HisAla: 2.2 ± 0.052
0.189HisCys: 0.189 ± 0.016
2.02HisAsp: 2.02 ± 0.049
1.728HisGlu: 1.728 ± 0.051
0.605HisPhe: 0.605 ± 0.025
1.993HisGly: 1.993 ± 0.044
0.564HisHis: 0.564 ± 0.028
0.673HisIle: 0.673 ± 0.029
0.294HisLys: 0.294 ± 0.018
1.798HisLeu: 1.798 ± 0.048
0.292HisMet: 0.292 ± 0.017
0.456HisAsn: 0.456 ± 0.022
1.276HisPro: 1.276 ± 0.04
0.493HisGln: 0.493 ± 0.023
1.326HisArg: 1.326 ± 0.042
0.866HisSer: 0.866 ± 0.035
1.063HisThr: 1.063 ± 0.035
2.171HisVal: 2.171 ± 0.051
0.239HisTrp: 0.239 ± 0.016
0.621HisTyr: 0.621 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
4.137IleAla: 4.137 ± 0.074
0.231IleCys: 0.231 ± 0.016
3.206IleAsp: 3.206 ± 0.073
3.468IleGlu: 3.468 ± 0.071
0.823IlePhe: 0.823 ± 0.031
3.186IleGly: 3.186 ± 0.069
0.789IleHis: 0.789 ± 0.031
1.069IleIle: 1.069 ± 0.042
0.574IleLys: 0.574 ± 0.027
2.655IleLeu: 2.655 ± 0.062
0.381IleMet: 0.381 ± 0.022
0.827IleAsn: 0.827 ± 0.033
1.906IlePro: 1.906 ± 0.047
1.1IleGln: 1.1 ± 0.036
2.272IleArg: 2.272 ± 0.045
1.877IleSer: 1.877 ± 0.049
2.079IleThr: 2.079 ± 0.051
3.067IleVal: 3.067 ± 0.073
0.288IleTrp: 0.288 ± 0.018
0.78IleTyr: 0.78 ± 0.027
0.0IleXaa: 0.0 ± 0.0
Lys
1.665LysAla: 1.665 ± 0.052
0.117LysCys: 0.117 ± 0.012
1.1LysAsp: 1.1 ± 0.037
1.124LysGlu: 1.124 ± 0.044
0.541LysPhe: 0.541 ± 0.026
1.209LysGly: 1.209 ± 0.044
0.502LysHis: 0.502 ± 0.026
0.667LysIle: 0.667 ± 0.027
0.418LysLys: 0.418 ± 0.025
1.707LysLeu: 1.707 ± 0.049
0.326LysMet: 0.326 ± 0.017
0.517LysAsn: 0.517 ± 0.03
0.874LysPro: 0.874 ± 0.031
0.759LysGln: 0.759 ± 0.03
1.527LysArg: 1.527 ± 0.042
1.053LysSer: 1.053 ± 0.039
1.286LysThr: 1.286 ± 0.043
1.198LysVal: 1.198 ± 0.037
0.223LysTrp: 0.223 ± 0.017
0.617LysTyr: 0.617 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
11.281LeuAla: 11.281 ± 0.148
0.484LeuCys: 0.484 ± 0.023
7.779LeuAsp: 7.779 ± 0.113
6.538LeuGlu: 6.538 ± 0.101
3.065LeuPhe: 3.065 ± 0.072
7.427LeuGly: 7.427 ± 0.1
1.613LeuHis: 1.613 ± 0.038
2.19LeuIle: 2.19 ± 0.052
1.448LeuLys: 1.448 ± 0.04
8.259LeuLeu: 8.259 ± 0.135
1.244LeuMet: 1.244 ± 0.038
1.807LeuAsn: 1.807 ± 0.048
4.098LeuPro: 4.098 ± 0.067
2.258LeuGln: 2.258 ± 0.05
5.459LeuArg: 5.459 ± 0.086
5.327LeuSer: 5.327 ± 0.07
5.195LeuThr: 5.195 ± 0.084
8.792LeuVal: 8.792 ± 0.118
0.841LeuTrp: 0.841 ± 0.034
2.108LeuTyr: 2.108 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
1.808MetAla: 1.808 ± 0.047
0.099MetCys: 0.099 ± 0.01
1.323MetAsp: 1.323 ± 0.039
1.131MetGlu: 1.131 ± 0.037
0.515MetPhe: 0.515 ± 0.024
1.334MetGly: 1.334 ± 0.044
0.37MetHis: 0.37 ± 0.021
0.497MetIle: 0.497 ± 0.027
0.392MetLys: 0.392 ± 0.021
1.526MetLeu: 1.526 ± 0.04
0.263MetMet: 0.263 ± 0.018
0.574MetAsn: 0.574 ± 0.026
0.892MetPro: 0.892 ± 0.029
0.553MetGln: 0.553 ± 0.023
1.093MetArg: 1.093 ± 0.034
1.459MetSer: 1.459 ± 0.044
1.491MetThr: 1.491 ± 0.041
1.325MetVal: 1.325 ± 0.041
0.162MetTrp: 0.162 ± 0.013
0.417MetTyr: 0.417 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
2.804AsnAla: 2.804 ± 0.066
0.196AsnCys: 0.196 ± 0.015
1.866AsnAsp: 1.866 ± 0.052
1.67AsnGlu: 1.67 ± 0.049
0.738AsnPhe: 0.738 ± 0.03
2.012AsnGly: 2.012 ± 0.057
0.505AsnHis: 0.505 ± 0.023
0.909AsnIle: 0.909 ± 0.038
0.449AsnLys: 0.449 ± 0.024
1.992AsnLeu: 1.992 ± 0.055
0.389AsnMet: 0.389 ± 0.019
0.618AsnAsn: 0.618 ± 0.034
1.603AsnPro: 1.603 ± 0.043
0.646AsnGln: 0.646 ± 0.027
1.434AsnArg: 1.434 ± 0.045
1.094AsnSer: 1.094 ± 0.041
1.351AsnThr: 1.351 ± 0.038
2.671AsnVal: 2.671 ± 0.054
0.308AsnTrp: 0.308 ± 0.02
0.688AsnTyr: 0.688 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
4.751ProAla: 4.751 ± 0.074
0.221ProCys: 0.221 ± 0.016
4.918ProAsp: 4.918 ± 0.082
4.502ProGlu: 4.502 ± 0.07
1.568ProPhe: 1.568 ± 0.05
4.037ProGly: 4.037 ± 0.084
0.95ProHis: 0.95 ± 0.03
1.62ProIle: 1.62 ± 0.044
0.843ProLys: 0.843 ± 0.031
3.655ProLeu: 3.655 ± 0.073
0.863ProMet: 0.863 ± 0.03
1.14ProAsn: 1.14 ± 0.034
2.214ProPro: 2.214 ± 0.055
0.973ProGln: 0.973 ± 0.032
2.312ProArg: 2.312 ± 0.053
2.652ProSer: 2.652 ± 0.056
2.976ProThr: 2.976 ± 0.056
4.319ProVal: 4.319 ± 0.065
0.499ProTrp: 0.499 ± 0.023
1.112ProTyr: 1.112 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.587GlnAla: 2.587 ± 0.056
0.16GlnCys: 0.16 ± 0.013
1.673GlnAsp: 1.673 ± 0.047
1.688GlnGlu: 1.688 ± 0.049
1.139GlnPhe: 1.139 ± 0.031
1.596GlnGly: 1.596 ± 0.042
0.638GlnHis: 0.638 ± 0.03
0.959GlnIle: 0.959 ± 0.035
0.565GlnLys: 0.565 ± 0.025
2.23GlnLeu: 2.23 ± 0.052
0.608GlnMet: 0.608 ± 0.026
0.831GlnAsn: 0.831 ± 0.034
1.186GlnPro: 1.186 ± 0.038
1.079GlnGln: 1.079 ± 0.041
2.308GlnArg: 2.308 ± 0.052
1.736GlnSer: 1.736 ± 0.045
1.929GlnThr: 1.929 ± 0.054
1.931GlnVal: 1.931 ± 0.048
0.347GlnTrp: 0.347 ± 0.02
0.87GlnTyr: 0.87 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
6.355ArgAla: 6.355 ± 0.087
0.433ArgCys: 0.433 ± 0.026
5.485ArgAsp: 5.485 ± 0.084
6.26ArgGlu: 6.26 ± 0.112
2.172ArgPhe: 2.172 ± 0.051
4.525ArgGly: 4.525 ± 0.067
1.243ArgHis: 1.243 ± 0.041
2.604ArgIle: 2.604 ± 0.054
1.277ArgLys: 1.277 ± 0.044
5.449ArgLeu: 5.449 ± 0.077
1.202ArgMet: 1.202 ± 0.032
1.643ArgAsn: 1.643 ± 0.04
2.445ArgPro: 2.445 ± 0.051
1.846ArgGln: 1.846 ± 0.046
4.814ArgArg: 4.814 ± 0.095
3.299ArgSer: 3.299 ± 0.073
3.777ArgThr: 3.777 ± 0.068
5.778ArgVal: 5.778 ± 0.087
0.706ArgTrp: 0.706 ± 0.027
1.837ArgTyr: 1.837 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
5.227SerAla: 5.227 ± 0.079
0.304SerCys: 0.304 ± 0.022
4.553SerAsp: 4.553 ± 0.079
4.553SerGlu: 4.553 ± 0.074
2.035SerPhe: 2.035 ± 0.048
5.318SerGly: 5.318 ± 0.084
1.005SerHis: 1.005 ± 0.031
2.053SerIle: 2.053 ± 0.051
1.165SerLys: 1.165 ± 0.034
4.877SerLeu: 4.877 ± 0.076
1.088SerMet: 1.088 ± 0.041
1.386SerAsn: 1.386 ± 0.045
2.629SerPro: 2.629 ± 0.059
1.419SerGln: 1.419 ± 0.038
3.117SerArg: 3.117 ± 0.061
2.98SerSer: 2.98 ± 0.068
3.497SerThr: 3.497 ± 0.074
5.296SerVal: 5.296 ± 0.079
0.627SerTrp: 0.627 ± 0.025
1.473SerTyr: 1.473 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
6.989ThrAla: 6.989 ± 0.108
0.357ThrCys: 0.357 ± 0.024
5.649ThrAsp: 5.649 ± 0.092
4.624ThrGlu: 4.624 ± 0.084
2.125ThrPhe: 2.125 ± 0.052
5.641ThrGly: 5.641 ± 0.078
1.212ThrHis: 1.212 ± 0.038
2.678ThrIle: 2.678 ± 0.057
0.983ThrLys: 0.983 ± 0.033
5.649ThrLeu: 5.649 ± 0.085
1.01ThrMet: 1.01 ± 0.035
1.581ThrAsn: 1.581 ± 0.048
3.221ThrPro: 3.221 ± 0.055
1.407ThrGln: 1.407 ± 0.041
3.232ThrArg: 3.232 ± 0.07
3.128ThrSer: 3.128 ± 0.062
4.682ThrThr: 4.682 ± 0.107
7.002ThrVal: 7.002 ± 0.102
0.643ThrTrp: 0.643 ± 0.025
1.856ThrTyr: 1.856 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
11.4ValAla: 11.4 ± 0.18
0.683ValCys: 0.683 ± 0.031
8.54ValAsp: 8.54 ± 0.111
8.28ValGlu: 8.28 ± 0.124
3.298ValPhe: 3.298 ± 0.064
8.049ValGly: 8.049 ± 0.116
1.981ValHis: 1.981 ± 0.046
2.734ValIle: 2.734 ± 0.069
1.552ValLys: 1.552 ± 0.046
8.886ValLeu: 8.886 ± 0.12
1.427ValMet: 1.427 ± 0.044
2.262ValAsn: 2.262 ± 0.051
4.501ValPro: 4.501 ± 0.074
2.308ValGln: 2.308 ± 0.051
5.764ValArg: 5.764 ± 0.078
5.408ValSer: 5.408 ± 0.093
6.748ValThr: 6.748 ± 0.102
10.956ValVal: 10.956 ± 0.141
0.937ValTrp: 0.937 ± 0.033
2.369ValTyr: 2.369 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
0.986TrpAla: 0.986 ± 0.034
0.104TrpCys: 0.104 ± 0.012
0.787TrpAsp: 0.787 ± 0.033
0.824TrpGlu: 0.824 ± 0.034
0.46TrpPhe: 0.46 ± 0.023
0.859TrpGly: 0.859 ± 0.039
0.241TrpHis: 0.241 ± 0.018
0.391TrpIle: 0.391 ± 0.022
0.286TrpLys: 0.286 ± 0.018
1.231TrpLeu: 1.231 ± 0.042
0.211TrpMet: 0.211 ± 0.014
0.331TrpAsn: 0.331 ± 0.02
0.467TrpPro: 0.467 ± 0.024
0.357TrpGln: 0.357 ± 0.018
0.865TrpArg: 0.865 ± 0.036
0.61TrpSer: 0.61 ± 0.028
0.827TrpThr: 0.827 ± 0.033
0.977TrpVal: 0.977 ± 0.033
0.218TrpTrp: 0.218 ± 0.015
0.364TrpTyr: 0.364 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.86TyrAla: 2.86 ± 0.051
0.268TyrCys: 0.268 ± 0.017
2.73TyrAsp: 2.73 ± 0.068
2.623TyrGlu: 2.623 ± 0.057
0.928TyrPhe: 0.928 ± 0.037
2.459TyrGly: 2.459 ± 0.054
0.659TyrHis: 0.659 ± 0.027
0.665TyrIle: 0.665 ± 0.028
0.409TyrLys: 0.409 ± 0.021
2.354TyrLeu: 2.354 ± 0.053
0.397TyrMet: 0.397 ± 0.02
0.677TyrAsn: 0.677 ± 0.03
1.23TyrPro: 1.23 ± 0.036
0.802TyrGln: 0.802 ± 0.027
1.88TyrArg: 1.88 ± 0.047
1.288TyrSer: 1.288 ± 0.036
1.528TyrThr: 1.528 ± 0.044
2.654TyrVal: 2.654 ± 0.058
0.356TyrTrp: 0.356 ± 0.019
0.874TyrTyr: 0.874 ± 0.035
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3549 proteins (923842 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski