Streptomyces phage CricKo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bingvirus; unclassified Bingvirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3SW06|A0A6M3SW06_9CAUD Uncharacterized protein OS=Streptomyces phage CricKo OX=2725620 GN=24 PE=4 SV=1
MM1 pKa = 7.45TALTWDD7 pKa = 3.46QAGEE11 pKa = 4.05RR12 pKa = 11.84LFEE15 pKa = 4.19TGVDD19 pKa = 3.36HH20 pKa = 7.0GVLYY24 pKa = 10.59IPDD27 pKa = 3.54NVGAYY32 pKa = 10.08VEE34 pKa = 5.03GYY36 pKa = 9.52AWNGLTAVTEE46 pKa = 4.43SPSGAEE52 pKa = 4.33SNPQYY57 pKa = 11.32ADD59 pKa = 3.31NIKK62 pKa = 10.35YY63 pKa = 10.59LNLVSAEE70 pKa = 4.09EE71 pKa = 3.98FGGTIEE77 pKa = 4.71AFTYY81 pKa = 10.2PDD83 pKa = 3.98EE84 pKa = 5.36FGQCDD89 pKa = 3.94GSASPTPGVSIGQQTRR105 pKa = 11.84KK106 pKa = 8.06TFGLSYY112 pKa = 8.99RR113 pKa = 11.84TKK115 pKa = 10.66VGNDD119 pKa = 3.33LAGQDD124 pKa = 3.57AGYY127 pKa = 10.48KK128 pKa = 9.67LHH130 pKa = 6.7LVYY133 pKa = 10.45GALAAPSEE141 pKa = 4.28KK142 pKa = 10.51AYY144 pKa = 9.79ATVNDD149 pKa = 4.02SPEE152 pKa = 4.83AITLSWEE159 pKa = 4.09FTTTPVEE166 pKa = 4.06VGVISGVTYY175 pKa = 10.65KK176 pKa = 9.51PTASLTIDD184 pKa = 3.48STKK187 pKa = 10.48VDD189 pKa = 3.67AEE191 pKa = 3.98ALEE194 pKa = 4.14TLEE197 pKa = 3.98EE198 pKa = 4.21FLYY201 pKa = 9.07GTAGTDD207 pKa = 3.36PSLPSPAAVVAMFSGTVLTATPTEE231 pKa = 3.97PAYY234 pKa = 10.92DD235 pKa = 3.54NATNTLTIPTVTGVTYY251 pKa = 9.68YY252 pKa = 10.29INGTAQVAGPVVLTEE267 pKa = 3.98NVIVEE272 pKa = 4.12ARR274 pKa = 11.84PNLGYY279 pKa = 10.8KK280 pKa = 10.18FPSNVDD286 pKa = 3.32TDD288 pKa = 3.67WAKK291 pKa = 11.43GDD293 pKa = 3.35II294 pKa = 4.14

Molecular weight: 31.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3SWN2|A0A6M3SWN2_9CAUD Uncharacterized protein OS=Streptomyces phage CricKo OX=2725620 GN=86 PE=4 SV=1
MM1 pKa = 7.39NEE3 pKa = 3.87SNIIAVVLIVSAGVIAAAAGIDD25 pKa = 4.43AGLSWWSEE33 pKa = 3.85RR34 pKa = 11.84PAKK37 pKa = 9.56PKK39 pKa = 8.98KK40 pKa = 9.54AKK42 pKa = 10.26YY43 pKa = 8.5VATMPRR49 pKa = 11.84KK50 pKa = 9.76IPTCSTSQQNLEE62 pKa = 4.09TLEE65 pKa = 4.52LGLMRR70 pKa = 11.84IQQRR74 pKa = 11.84MHH76 pKa = 7.02RR77 pKa = 11.84SHH79 pKa = 6.63GAHH82 pKa = 6.14RR83 pKa = 11.84RR84 pKa = 11.84IKK86 pKa = 9.76TGG88 pKa = 2.93

Molecular weight: 9.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

18181

31

1825

193.4

21.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.07 ± 0.428

0.633 ± 0.105

6.171 ± 0.307

7.123 ± 0.359

3.773 ± 0.203

7.689 ± 0.45

1.727 ± 0.191

5.484 ± 0.203

6.457 ± 0.326

8.025 ± 0.444

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.607 ± 0.117

3.933 ± 0.16

4.224 ± 0.276

3.146 ± 0.142

5.28 ± 0.351

6.012 ± 0.206

6.556 ± 0.362

7.183 ± 0.206

1.573 ± 0.166

3.333 ± 0.242
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski