Amino acid dipepetide frequency for Streptomyces phage CricKo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.066AlaAla: 10.066 ± 1.221
0.495AlaCys: 0.495 ± 0.181
4.895AlaAsp: 4.895 ± 0.679
6.326AlaGlu: 6.326 ± 0.524
2.475AlaPhe: 2.475 ± 0.324
7.921AlaGly: 7.921 ± 1.295
1.21AlaHis: 1.21 ± 0.292
4.84AlaIle: 4.84 ± 0.667
7.206AlaLys: 7.206 ± 0.734
7.591AlaLeu: 7.591 ± 1.328
2.97AlaMet: 2.97 ± 0.383
2.915AlaAsn: 2.915 ± 0.525
3.08AlaPro: 3.08 ± 0.427
3.025AlaGln: 3.025 ± 0.548
4.73AlaArg: 4.73 ± 0.678
4.675AlaSer: 4.675 ± 0.661
5.116AlaThr: 5.116 ± 0.514
6.711AlaVal: 6.711 ± 0.498
1.485AlaTrp: 1.485 ± 0.338
2.97AlaTyr: 2.97 ± 0.323
0.0AlaXaa: 0.0 ± 0.0
Cys
0.495CysAla: 0.495 ± 0.202
0.22CysCys: 0.22 ± 0.105
0.33CysAsp: 0.33 ± 0.124
0.165CysGlu: 0.165 ± 0.087
0.11CysPhe: 0.11 ± 0.075
0.55CysGly: 0.55 ± 0.207
0.11CysHis: 0.11 ± 0.078
0.495CysIle: 0.495 ± 0.175
0.22CysLys: 0.22 ± 0.106
0.66CysLeu: 0.66 ± 0.223
0.055CysMet: 0.055 ± 0.06
0.495CysAsn: 0.495 ± 0.193
0.22CysPro: 0.22 ± 0.109
0.11CysGln: 0.11 ± 0.09
0.055CysArg: 0.055 ± 0.049
0.825CysSer: 0.825 ± 0.22
0.385CysThr: 0.385 ± 0.162
0.495CysVal: 0.495 ± 0.205
0.165CysTrp: 0.165 ± 0.089
0.165CysTyr: 0.165 ± 0.087
0.0CysXaa: 0.0 ± 0.0
Asp
5.446AspAla: 5.446 ± 0.643
0.55AspCys: 0.55 ± 0.186
3.245AspAsp: 3.245 ± 0.549
5.776AspGlu: 5.776 ± 0.755
2.2AspPhe: 2.2 ± 0.394
5.776AspGly: 5.776 ± 0.685
0.88AspHis: 0.88 ± 0.26
2.805AspIle: 2.805 ± 0.476
2.86AspLys: 2.86 ± 0.401
4.455AspLeu: 4.455 ± 0.488
1.76AspMet: 1.76 ± 0.27
2.09AspAsn: 2.09 ± 0.407
3.905AspPro: 3.905 ± 0.677
2.2AspGln: 2.2 ± 0.324
3.025AspArg: 3.025 ± 0.493
3.905AspSer: 3.905 ± 0.485
3.795AspThr: 3.795 ± 0.385
4.235AspVal: 4.235 ± 0.471
1.045AspTrp: 1.045 ± 0.234
1.76AspTyr: 1.76 ± 0.347
0.0AspXaa: 0.0 ± 0.0
Glu
4.73GluAla: 4.73 ± 0.49
0.385GluCys: 0.385 ± 0.177
4.62GluAsp: 4.62 ± 0.664
6.601GluGlu: 6.601 ± 1.037
3.795GluPhe: 3.795 ± 0.571
4.235GluGly: 4.235 ± 0.55
1.485GluHis: 1.485 ± 0.356
4.125GluIle: 4.125 ± 0.591
5.006GluLys: 5.006 ± 0.493
5.941GluLeu: 5.941 ± 0.467
2.53GluMet: 2.53 ± 0.323
2.42GluAsn: 2.42 ± 0.375
2.64GluPro: 2.64 ± 0.504
2.145GluGln: 2.145 ± 0.369
4.51GluArg: 4.51 ± 0.569
3.41GluSer: 3.41 ± 0.496
4.73GluThr: 4.73 ± 0.649
4.675GluVal: 4.675 ± 0.669
1.32GluTrp: 1.32 ± 0.257
1.925GluTyr: 1.925 ± 0.293
0.0GluXaa: 0.0 ± 0.0
Phe
2.86PheAla: 2.86 ± 0.381
0.22PheCys: 0.22 ± 0.13
3.135PheAsp: 3.135 ± 0.498
2.64PheGlu: 2.64 ± 0.299
1.54PhePhe: 1.54 ± 0.265
2.97PheGly: 2.97 ± 0.531
0.605PheHis: 0.605 ± 0.215
2.09PheIle: 2.09 ± 0.338
2.145PheLys: 2.145 ± 0.395
2.915PheLeu: 2.915 ± 0.441
1.1PheMet: 1.1 ± 0.196
1.65PheAsn: 1.65 ± 0.333
1.1PhePro: 1.1 ± 0.209
1.76PheGln: 1.76 ± 0.27
1.87PheArg: 1.87 ± 0.33
2.2PheSer: 2.2 ± 0.441
2.53PheThr: 2.53 ± 0.447
2.585PheVal: 2.585 ± 0.414
0.605PheTrp: 0.605 ± 0.218
1.21PheTyr: 1.21 ± 0.278
0.0PheXaa: 0.0 ± 0.0
Gly
7.976GlyAla: 7.976 ± 0.825
0.385GlyCys: 0.385 ± 0.153
5.391GlyAsp: 5.391 ± 0.496
4.565GlyGlu: 4.565 ± 0.543
3.52GlyPhe: 3.52 ± 0.595
4.51GlyGly: 4.51 ± 0.646
1.155GlyHis: 1.155 ± 0.225
5.061GlyIle: 5.061 ± 0.857
4.07GlyLys: 4.07 ± 0.494
6.711GlyLeu: 6.711 ± 1.518
1.815GlyMet: 1.815 ± 0.27
2.145GlyAsn: 2.145 ± 0.327
2.915GlyPro: 2.915 ± 0.437
1.595GlyGln: 1.595 ± 0.294
3.3GlyArg: 3.3 ± 0.345
4.345GlySer: 4.345 ± 0.47
5.666GlyThr: 5.666 ± 0.566
6.656GlyVal: 6.656 ± 0.63
1.595GlyTrp: 1.595 ± 0.357
3.52GlyTyr: 3.52 ± 0.587
0.0GlyXaa: 0.0 ± 0.0
His
1.155HisAla: 1.155 ± 0.298
0.33HisCys: 0.33 ± 0.143
0.935HisAsp: 0.935 ± 0.257
0.825HisGlu: 0.825 ± 0.234
0.66HisPhe: 0.66 ± 0.224
1.705HisGly: 1.705 ± 0.346
0.605HisHis: 0.605 ± 0.18
0.935HisIle: 0.935 ± 0.236
0.77HisLys: 0.77 ± 0.229
1.375HisLeu: 1.375 ± 0.316
0.55HisMet: 0.55 ± 0.156
0.385HisAsn: 0.385 ± 0.133
0.825HisPro: 0.825 ± 0.215
0.55HisGln: 0.55 ± 0.209
1.1HisArg: 1.1 ± 0.26
1.43HisSer: 1.43 ± 0.295
0.715HisThr: 0.715 ± 0.165
1.375HisVal: 1.375 ± 0.299
0.165HisTrp: 0.165 ± 0.092
0.88HisTyr: 0.88 ± 0.204
0.0HisXaa: 0.0 ± 0.0
Ile
5.556IleAla: 5.556 ± 0.557
0.22IleCys: 0.22 ± 0.131
3.465IleAsp: 3.465 ± 0.328
4.29IleGlu: 4.29 ± 0.521
2.75IlePhe: 2.75 ± 0.418
4.895IleGly: 4.895 ± 0.955
1.045IleHis: 1.045 ± 0.216
1.98IleIle: 1.98 ± 0.414
3.3IleLys: 3.3 ± 0.453
4.675IleLeu: 4.675 ± 0.46
1.155IleMet: 1.155 ± 0.207
1.815IleAsn: 1.815 ± 0.282
2.365IlePro: 2.365 ± 0.299
1.815IleGln: 1.815 ± 0.361
2.75IleArg: 2.75 ± 0.435
3.025IleSer: 3.025 ± 0.448
3.96IleThr: 3.96 ± 0.524
3.63IleVal: 3.63 ± 0.451
0.77IleTrp: 0.77 ± 0.211
1.375IleTyr: 1.375 ± 0.242
0.0IleXaa: 0.0 ± 0.0
Lys
6.491LysAla: 6.491 ± 0.782
0.385LysCys: 0.385 ± 0.138
2.64LysAsp: 2.64 ± 0.462
3.905LysGlu: 3.905 ± 0.435
1.65LysPhe: 1.65 ± 0.361
4.07LysGly: 4.07 ± 0.64
0.88LysHis: 0.88 ± 0.276
3.74LysIle: 3.74 ± 0.378
5.281LysLys: 5.281 ± 0.738
5.721LysLeu: 5.721 ± 0.689
2.035LysMet: 2.035 ± 0.35
3.19LysAsn: 3.19 ± 0.483
3.08LysPro: 3.08 ± 0.487
1.87LysGln: 1.87 ± 0.303
3.245LysArg: 3.245 ± 0.583
4.07LysSer: 4.07 ± 0.563
4.235LysThr: 4.235 ± 0.672
4.895LysVal: 4.895 ± 0.56
0.935LysTrp: 0.935 ± 0.24
2.255LysTyr: 2.255 ± 0.357
0.0LysXaa: 0.0 ± 0.0
Leu
7.866LeuAla: 7.866 ± 1.311
0.33LeuCys: 0.33 ± 0.153
4.895LeuAsp: 4.895 ± 0.567
5.831LeuGlu: 5.831 ± 0.743
2.97LeuPhe: 2.97 ± 0.44
6.876LeuGly: 6.876 ± 1.278
1.045LeuHis: 1.045 ± 0.24
4.235LeuIle: 4.235 ± 0.484
5.611LeuLys: 5.611 ± 0.886
5.391LeuLeu: 5.391 ± 0.845
2.365LeuMet: 2.365 ± 0.342
3.905LeuAsn: 3.905 ± 0.475
2.75LeuPro: 2.75 ± 0.344
2.75LeuGln: 2.75 ± 0.461
4.125LeuArg: 4.125 ± 0.512
5.391LeuSer: 5.391 ± 0.778
5.721LeuThr: 5.721 ± 0.741
4.785LeuVal: 4.785 ± 0.544
0.99LeuTrp: 0.99 ± 0.261
2.42LeuTyr: 2.42 ± 0.356
0.0LeuXaa: 0.0 ± 0.0
Met
2.64MetAla: 2.64 ± 0.43
0.275MetCys: 0.275 ± 0.119
1.595MetAsp: 1.595 ± 0.311
1.98MetGlu: 1.98 ± 0.462
0.66MetPhe: 0.66 ± 0.235
1.54MetGly: 1.54 ± 0.247
0.605MetHis: 0.605 ± 0.162
1.595MetIle: 1.595 ± 0.32
1.925MetLys: 1.925 ± 0.357
2.035MetLeu: 2.035 ± 0.409
0.385MetMet: 0.385 ± 0.148
1.155MetAsn: 1.155 ± 0.277
1.265MetPro: 1.265 ± 0.263
0.715MetGln: 0.715 ± 0.204
1.155MetArg: 1.155 ± 0.267
2.09MetSer: 2.09 ± 0.297
1.98MetThr: 1.98 ± 0.308
1.375MetVal: 1.375 ± 0.275
0.22MetTrp: 0.22 ± 0.101
0.88MetTyr: 0.88 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
4.125AsnAla: 4.125 ± 0.431
0.22AsnCys: 0.22 ± 0.147
2.035AsnAsp: 2.035 ± 0.347
2.64AsnGlu: 2.64 ± 0.383
1.76AsnPhe: 1.76 ± 0.324
3.52AsnGly: 3.52 ± 0.447
0.715AsnHis: 0.715 ± 0.172
1.595AsnIle: 1.595 ± 0.304
2.75AsnLys: 2.75 ± 0.44
2.915AsnLeu: 2.915 ± 0.43
0.55AsnMet: 0.55 ± 0.237
1.65AsnAsn: 1.65 ± 0.315
2.365AsnPro: 2.365 ± 0.407
1.485AsnGln: 1.485 ± 0.253
2.365AsnArg: 2.365 ± 0.439
2.2AsnSer: 2.2 ± 0.384
1.925AsnThr: 1.925 ± 0.299
2.64AsnVal: 2.64 ± 0.353
0.55AsnTrp: 0.55 ± 0.187
1.32AsnTyr: 1.32 ± 0.245
0.0AsnXaa: 0.0 ± 0.0
Pro
3.3ProAla: 3.3 ± 0.514
0.495ProCys: 0.495 ± 0.184
3.41ProAsp: 3.41 ± 0.537
3.74ProGlu: 3.74 ± 0.552
1.32ProPhe: 1.32 ± 0.279
2.695ProGly: 2.695 ± 0.368
0.825ProHis: 0.825 ± 0.305
1.54ProIle: 1.54 ± 0.274
2.915ProLys: 2.915 ± 0.448
2.86ProLeu: 2.86 ± 0.458
1.1ProMet: 1.1 ± 0.235
2.255ProAsn: 2.255 ± 0.358
1.65ProPro: 1.65 ± 0.339
1.1ProGln: 1.1 ± 0.253
2.365ProArg: 2.365 ± 0.527
2.64ProSer: 2.64 ± 0.444
2.64ProThr: 2.64 ± 0.5
3.575ProVal: 3.575 ± 0.459
0.66ProTrp: 0.66 ± 0.249
1.155ProTyr: 1.155 ± 0.264
0.0ProXaa: 0.0 ± 0.0
Gln
3.025GlnAla: 3.025 ± 0.417
0.165GlnCys: 0.165 ± 0.093
1.87GlnAsp: 1.87 ± 0.302
1.32GlnGlu: 1.32 ± 0.232
1.265GlnPhe: 1.265 ± 0.249
2.365GlnGly: 2.365 ± 0.384
0.88GlnHis: 0.88 ± 0.24
2.2GlnIle: 2.2 ± 0.32
2.035GlnLys: 2.035 ± 0.372
2.475GlnLeu: 2.475 ± 0.469
0.935GlnMet: 0.935 ± 0.208
1.045GlnAsn: 1.045 ± 0.279
1.045GlnPro: 1.045 ± 0.239
1.815GlnGln: 1.815 ± 0.353
1.65GlnArg: 1.65 ± 0.293
1.87GlnSer: 1.87 ± 0.25
2.09GlnThr: 2.09 ± 0.406
2.145GlnVal: 2.145 ± 0.294
0.33GlnTrp: 0.33 ± 0.136
0.935GlnTyr: 0.935 ± 0.219
0.0GlnXaa: 0.0 ± 0.0
Arg
3.245ArgAla: 3.245 ± 0.469
0.275ArgCys: 0.275 ± 0.107
3.52ArgAsp: 3.52 ± 0.405
4.235ArgGlu: 4.235 ± 0.558
1.65ArgPhe: 1.65 ± 0.247
2.64ArgGly: 2.64 ± 0.422
0.825ArgHis: 0.825 ± 0.257
2.97ArgIle: 2.97 ± 0.529
3.795ArgLys: 3.795 ± 0.514
4.95ArgLeu: 4.95 ± 0.617
2.145ArgMet: 2.145 ± 0.343
2.365ArgAsn: 2.365 ± 0.335
2.145ArgPro: 2.145 ± 0.478
1.705ArgGln: 1.705 ± 0.309
3.85ArgArg: 3.85 ± 0.622
3.245ArgSer: 3.245 ± 0.567
3.355ArgThr: 3.355 ± 0.568
3.575ArgVal: 3.575 ± 0.522
0.605ArgTrp: 0.605 ± 0.206
1.705ArgTyr: 1.705 ± 0.369
0.0ArgXaa: 0.0 ± 0.0
Ser
5.281SerAla: 5.281 ± 0.812
0.11SerCys: 0.11 ± 0.081
3.905SerAsp: 3.905 ± 0.485
3.52SerGlu: 3.52 ± 0.438
2.035SerPhe: 2.035 ± 0.322
6.271SerGly: 6.271 ± 0.635
0.935SerHis: 0.935 ± 0.261
3.135SerIle: 3.135 ± 0.38
3.19SerLys: 3.19 ± 0.484
5.446SerLeu: 5.446 ± 0.628
1.21SerMet: 1.21 ± 0.259
1.98SerAsn: 1.98 ± 0.32
3.025SerPro: 3.025 ± 0.431
1.54SerGln: 1.54 ± 0.282
3.245SerArg: 3.245 ± 0.503
4.29SerSer: 4.29 ± 0.668
3.85SerThr: 3.85 ± 0.511
4.07SerVal: 4.07 ± 0.481
1.21SerTrp: 1.21 ± 0.2
1.87SerTyr: 1.87 ± 0.29
0.0SerXaa: 0.0 ± 0.0
Thr
5.611ThrAla: 5.611 ± 0.677
0.33ThrCys: 0.33 ± 0.19
3.685ThrAsp: 3.685 ± 0.541
4.565ThrGlu: 4.565 ± 0.574
2.86ThrPhe: 2.86 ± 0.417
5.611ThrGly: 5.611 ± 0.845
1.21ThrHis: 1.21 ± 0.27
4.455ThrIle: 4.455 ± 0.453
3.96ThrLys: 3.96 ± 0.462
5.336ThrLeu: 5.336 ± 0.504
0.88ThrMet: 0.88 ± 0.171
2.09ThrAsn: 2.09 ± 0.309
3.355ThrPro: 3.355 ± 0.562
2.255ThrGln: 2.255 ± 0.469
3.08ThrArg: 3.08 ± 0.415
2.805ThrSer: 2.805 ± 0.512
4.785ThrThr: 4.785 ± 0.795
5.776ThrVal: 5.776 ± 0.743
0.935ThrTrp: 0.935 ± 0.219
1.98ThrTyr: 1.98 ± 0.404
0.0ThrXaa: 0.0 ± 0.0
Val
6.106ValAla: 6.106 ± 0.464
0.55ValCys: 0.55 ± 0.182
4.455ValAsp: 4.455 ± 0.411
5.391ValGlu: 5.391 ± 0.674
2.365ValPhe: 2.365 ± 0.397
5.281ValGly: 5.281 ± 0.555
1.21ValHis: 1.21 ± 0.301
4.73ValIle: 4.73 ± 0.427
4.675ValLys: 4.675 ± 0.35
5.556ValLeu: 5.556 ± 0.562
1.54ValMet: 1.54 ± 0.28
2.75ValAsn: 2.75 ± 0.303
2.53ValPro: 2.53 ± 0.375
1.76ValGln: 1.76 ± 0.393
4.015ValArg: 4.015 ± 0.55
4.29ValSer: 4.29 ± 0.423
5.721ValThr: 5.721 ± 0.65
4.84ValVal: 4.84 ± 0.526
1.045ValTrp: 1.045 ± 0.221
2.97ValTyr: 2.97 ± 0.526
0.0ValXaa: 0.0 ± 0.0
Trp
1.485TrpAla: 1.485 ± 0.328
0.22TrpCys: 0.22 ± 0.116
1.1TrpAsp: 1.1 ± 0.298
1.155TrpGlu: 1.155 ± 0.204
0.66TrpPhe: 0.66 ± 0.186
1.21TrpGly: 1.21 ± 0.299
0.275TrpHis: 0.275 ± 0.114
0.825TrpIle: 0.825 ± 0.259
0.715TrpLys: 0.715 ± 0.251
1.1TrpLeu: 1.1 ± 0.287
0.275TrpMet: 0.275 ± 0.122
1.375TrpAsn: 1.375 ± 0.298
0.33TrpPro: 0.33 ± 0.122
0.22TrpGln: 0.22 ± 0.1
0.715TrpArg: 0.715 ± 0.247
1.21TrpSer: 1.21 ± 0.354
0.88TrpThr: 0.88 ± 0.309
1.155TrpVal: 1.155 ± 0.263
0.385TrpTrp: 0.385 ± 0.139
0.44TrpTyr: 0.44 ± 0.179
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.245TyrAla: 3.245 ± 0.415
0.165TyrCys: 0.165 ± 0.097
2.585TyrAsp: 2.585 ± 0.387
1.76TyrGlu: 1.76 ± 0.423
1.485TyrPhe: 1.485 ± 0.301
2.255TyrGly: 2.255 ± 0.372
0.77TyrHis: 0.77 ± 0.242
1.485TyrIle: 1.485 ± 0.292
2.145TyrLys: 2.145 ± 0.513
2.145TyrLeu: 2.145 ± 0.352
0.66TyrMet: 0.66 ± 0.21
1.65TyrAsn: 1.65 ± 0.273
1.65TyrPro: 1.65 ± 0.298
1.045TyrGln: 1.045 ± 0.205
1.65TyrArg: 1.65 ± 0.297
2.2TyrSer: 2.2 ± 0.309
1.485TyrThr: 1.485 ± 0.351
2.64TyrVal: 2.64 ± 0.304
0.715TyrTrp: 0.715 ± 0.207
1.595TyrTyr: 1.595 ± 0.372
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (18181 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski