Listeria phage LP-HM00113468
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7D4XKJ3|A0A7D4XKJ3_9CAUD Uncharacterized protein OS=Listeria phage LP-HM00113468 OX=2744802 PE=4 SV=1
MM1 pKa = 7.39 TFYY4 pKa = 11.31 DD5 pKa = 4.76 FLITYY10 pKa = 7.6 YY11 pKa = 10.8 LSEE14 pKa = 4.39 NSPLGDD20 pKa = 3.58 LAHH23 pKa = 7.48 DD24 pKa = 3.91 VQLDD28 pKa = 3.86 GNFPTEE34 pKa = 4.28 SKK36 pKa = 10.92 SEE38 pKa = 4.04 DD39 pKa = 3.5 EE40 pKa = 3.96 IRR42 pKa = 11.84 DD43 pKa = 3.63 YY44 pKa = 10.98 FSNIGTPGFQEE55 pKa = 4.68 ALDD58 pKa = 3.73 EE59 pKa = 4.35 ALNYY63 pKa = 9.26 FRR65 pKa = 11.84 RR66 pKa = 11.84 LL67 pKa = 3.28
Molecular weight: 7.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.792
IPC2_protein 4.012
IPC_protein 3.91
Toseland 3.719
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.834
Nozaki 4.024
DTASelect 4.202
Thurlkill 3.783
EMBOSS 3.834
Sillero 4.024
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.923
Protein with the highest isoelectric point:
>tr|A0A7D5BK37|A0A7D5BK37_9CAUD Uncharacterized protein OS=Listeria phage LP-HM00113468 OX=2744802 PE=4 SV=1
MM1 pKa = 6.99 TSYY4 pKa = 11.05 YY5 pKa = 10.42 YY6 pKa = 10.61 SRR8 pKa = 11.84 SLANVNKK15 pKa = 10.25 LADD18 pKa = 3.78 NTKK21 pKa = 9.24 VAARR25 pKa = 11.84 KK26 pKa = 9.61 LLDD29 pKa = 3.09 WAEE32 pKa = 3.93 NSGIEE37 pKa = 3.99 VLIYY41 pKa = 8.37 EE42 pKa = 4.8 TIRR45 pKa = 11.84 TKK47 pKa = 10.13 EE48 pKa = 3.77 QQSANVASGASQTMRR63 pKa = 11.84 SYY65 pKa = 11.3 HH66 pKa = 6.34 LVGQALDD73 pKa = 3.54 FVMAKK78 pKa = 10.23 GKK80 pKa = 7.13 TVNWGGYY87 pKa = 9.04 RR88 pKa = 11.84 SANAKK93 pKa = 10.22 KK94 pKa = 10.34 FIAKK98 pKa = 10.04 AKK100 pKa = 10.44 ALGFTWGGDD109 pKa = 2.69 WDD111 pKa = 4.15 GFVDD115 pKa = 4.1 NPHH118 pKa = 6.53 LQFEE122 pKa = 4.69 YY123 pKa = 10.36 KK124 pKa = 10.58 GYY126 pKa = 8.59 GTDD129 pKa = 3.28 TFGKK133 pKa = 9.48 GASANVPAKK142 pKa = 10.53 PNTQSNSSLGLVDD155 pKa = 3.61 YY156 pKa = 11.2 MNMNKK161 pKa = 9.89 LDD163 pKa = 3.62 SSFANRR169 pKa = 11.84 KK170 pKa = 8.91 KK171 pKa = 10.51 LAAKK175 pKa = 10.29 YY176 pKa = 9.99 GIKK179 pKa = 10.25 NYY181 pKa = 10.77 SGTASQNTTLLAKK194 pKa = 10.48 LKK196 pKa = 10.2 AGKK199 pKa = 8.67 PHH201 pKa = 6.63 TPASNNTYY209 pKa = 8.55 YY210 pKa = 10.59 TEE212 pKa = 4.24 NPGKK216 pKa = 10.29 IKK218 pKa = 9.71 TLVQCDD224 pKa = 4.47 LYY226 pKa = 11.64 NSVDD230 pKa = 3.86 FTASHH235 pKa = 6.05 KK236 pKa = 9.79 TGGTYY241 pKa = 10.22 PPGTIFTIAGMAKK254 pKa = 9.25 TKK256 pKa = 10.69 GGTPRR261 pKa = 11.84 LKK263 pKa = 9.91 TKK265 pKa = 10.27 SGYY268 pKa = 10.56 FLTANKK274 pKa = 10.2 KK275 pKa = 8.79 FVKK278 pKa = 10.36 KK279 pKa = 10.34 II280 pKa = 3.41
Molecular weight: 30.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.073
IPC2_protein 9.414
IPC_protein 9.268
Toseland 10.014
ProMoST 9.663
Dawson 10.218
Bjellqvist 9.853
Wikipedia 10.365
Rodwell 10.804
Grimsley 10.277
Solomon 10.218
Lehninger 10.189
Nozaki 9.984
DTASelect 9.853
Thurlkill 10.058
EMBOSS 10.409
Sillero 10.116
Patrickios 10.35
IPC_peptide 10.218
IPC2_peptide 8.331
IPC2.peptide.svr19 8.372
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
11296
50
1420
209.2
23.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.905 ± 0.824
0.779 ± 0.142
6.356 ± 0.281
7.268 ± 0.433
4.497 ± 0.338
6.046 ± 0.624
1.567 ± 0.169
7.047 ± 0.32
9.03 ± 0.487
8.162 ± 0.285
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.505 ± 0.108
6.516 ± 0.197
2.62 ± 0.197
3.585 ± 0.222
3.718 ± 0.325
6.454 ± 0.371
5.63 ± 0.285
5.887 ± 0.243
1.292 ± 0.128
4.134 ± 0.375
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here