Penicillium decumbens
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7144 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V6PIW9|A0A1V6PIW9_PENDC MRP-L46 domain-containing protein OS=Penicillium decumbens OX=69771 GN=PENDEC_c004G04933 PE=3 SV=1
MM1 pKa = 7.63 SSTSPAGSNPSFSYY15 pKa = 10.31 SSYY18 pKa = 8.19 MTSSTQNAYY27 pKa = 10.49 GSGSDD32 pKa = 5.2 DD33 pKa = 4.61 EE34 pKa = 4.83 IASLPSVSTTDD45 pKa = 3.14 SDD47 pKa = 4.42 IDD49 pKa = 3.81 TLSDD53 pKa = 3.64 DD54 pKa = 4.25 FSDD57 pKa = 5.98 AEE59 pKa = 4.28 AEE61 pKa = 3.89 WRR63 pKa = 11.84 EE64 pKa = 4.21 SIEE67 pKa = 3.96 QLEE70 pKa = 4.64 LLLSMVLVPFIGKK83 pKa = 9.34 YY84 pKa = 9.64 LGRR87 pKa = 11.84 RR88 pKa = 11.84 CAYY91 pKa = 9.02 WGWTSFMQWKK101 pKa = 9.88 YY102 pKa = 9.0 PVEE105 pKa = 4.18 VVMTSPAAFKK115 pKa = 10.85 GAGLIEE121 pKa = 4.36 AAATLL126 pKa = 3.8
Molecular weight: 13.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.789
IPC2_protein 3.999
IPC_protein 3.91
Toseland 3.719
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.05
Wikipedia 3.808
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.821
Nozaki 4.012
DTASelect 4.19
Thurlkill 3.77
EMBOSS 3.821
Sillero 4.024
Patrickios 1.901
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.92
Protein with the highest isoelectric point:
>tr|A0A1V6PBN4|A0A1V6PBN4_PENDC Uncharacterized protein OS=Penicillium decumbens OX=69771 GN=PENDEC_c011G06140 PE=3 SV=1
MM1 pKa = 7.55 PRR3 pKa = 11.84 LRR5 pKa = 11.84 CRR7 pKa = 11.84 ALPTIRR13 pKa = 11.84 APVSSTTPMTRR24 pKa = 11.84 ILQFTPRR31 pKa = 11.84 TTSTPSILSSRR42 pKa = 11.84 PFSSLLSTPTRR53 pKa = 11.84 SQPSQSLSRR62 pKa = 11.84 LPFTSSPITSSVSSLLTQKK81 pKa = 10.41 PFQTRR86 pKa = 11.84 SFSATASLGVRR97 pKa = 11.84 RR98 pKa = 11.84 STYY101 pKa = 9.47 RR102 pKa = 11.84 PSRR105 pKa = 11.84 RR106 pKa = 11.84 IQKK109 pKa = 9.34 RR110 pKa = 11.84 RR111 pKa = 11.84 HH112 pKa = 5.33 GFLSRR117 pKa = 11.84 NKK119 pKa = 9.19 DD120 pKa = 3.12 HH121 pKa = 7.47 KK122 pKa = 10.92 GRR124 pKa = 11.84 KK125 pKa = 6.0 TLTRR129 pKa = 11.84 RR130 pKa = 11.84 RR131 pKa = 11.84 MKK133 pKa = 10.37 GRR135 pKa = 11.84 KK136 pKa = 8.84 AMSWW140 pKa = 3.05
Molecular weight: 15.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.432
IPC2_protein 11.023
IPC_protein 12.515
Toseland 12.676
ProMoST 13.173
Dawson 12.676
Bjellqvist 12.676
Wikipedia 13.159
Rodwell 12.31
Grimsley 12.72
Solomon 13.173
Lehninger 13.086
Nozaki 12.676
DTASelect 12.676
Thurlkill 12.676
EMBOSS 13.173
Sillero 12.676
Patrickios 12.032
IPC_peptide 13.188
IPC2_peptide 12.164
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7144
0
7144
3565102
8
6054
499.0
55.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.515 ± 0.024
1.124 ± 0.01
5.773 ± 0.021
6.289 ± 0.036
3.72 ± 0.019
6.759 ± 0.025
2.389 ± 0.012
4.883 ± 0.021
4.769 ± 0.027
8.841 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.264 ± 0.01
3.662 ± 0.016
6.223 ± 0.034
4.085 ± 0.02
6.195 ± 0.024
8.484 ± 0.036
5.824 ± 0.018
6.066 ± 0.022
1.4 ± 0.011
2.734 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here