Paenibacillus sambharensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4979 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W1L0M1|A0A2W1L0M1_9BACL Pyrimidine-nucleoside phosphorylase OS=Paenibacillus sambharensis OX=1803190 GN=deoA PE=3 SV=1
MM1 pKa = 7.87ANRR4 pKa = 11.84FLDD7 pKa = 3.88AQLSQARR14 pKa = 11.84SFPTATTTPVSAAGTQVATLGLNLTGAGPNAQVHH48 pKa = 6.19FDD50 pKa = 3.57FTAGFDD56 pKa = 3.61VDD58 pKa = 3.49ATDD61 pKa = 3.81PVGVTATVLRR71 pKa = 11.84DD72 pKa = 3.49GVPIYY77 pKa = 10.41QVIEE81 pKa = 4.08NFDD84 pKa = 3.9DD85 pKa = 4.67GVNQLLSFSGADD97 pKa = 3.68YY98 pKa = 10.8LPPAAFHH105 pKa = 7.04IYY107 pKa = 9.36TVVLAFTSADD117 pKa = 3.28PAAEE121 pKa = 3.92VDD123 pKa = 4.58LIGPVSFTAAGYY135 pKa = 10.88SNN137 pKa = 4.06

Molecular weight: 14.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W1LF93|A0A2W1LF93_9BACL NodB homology domain-containing protein OS=Paenibacillus sambharensis OX=1803190 GN=DNH61_22295 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.48GFRR19 pKa = 11.84KK20 pKa = 10.08RR21 pKa = 11.84MATKK25 pKa = 10.26NGRR28 pKa = 11.84KK29 pKa = 9.17VLSARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.26GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4979

0

4979

1622395

16

6310

325.8

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.989 ± 0.041

0.782 ± 0.012

5.156 ± 0.028

6.96 ± 0.038

3.838 ± 0.023

7.789 ± 0.032

2.112 ± 0.016

6.168 ± 0.031

4.681 ± 0.029

10.131 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.882 ± 0.016

3.432 ± 0.026

4.154 ± 0.021

3.892 ± 0.026

5.584 ± 0.034

6.202 ± 0.029

5.295 ± 0.029

7.148 ± 0.026

1.281 ± 0.014

3.526 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski