Lachnellula willkommii

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Lachnaceae; Lachnellula

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8978 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A559M0L4|A0A559M0L4_9HELO Glucan endo-1 3-beta-glucosidase A1 (Fragment) OS=Lachnellula willkommii OX=215461 GN=glcA_1 PE=4 SV=1
PP1 pKa = 7.44EE2 pKa = 4.66SVTDD6 pKa = 5.27GINAMITLIEE16 pKa = 4.81DD17 pKa = 3.93YY18 pKa = 10.58IGNCSSGNIVLTGFSQGAEE37 pKa = 4.14VVTDD41 pKa = 3.87TLAGGVDD48 pKa = 3.53KK49 pKa = 10.57PDD51 pKa = 4.08PLSAEE56 pKa = 3.81NVARR60 pKa = 11.84ISAVAVFGDD69 pKa = 3.64PTFTHH74 pKa = 6.73DD75 pKa = 3.55QSFDD79 pKa = 3.28AGTDD83 pKa = 3.2TSTDD87 pKa = 3.69GIFARR92 pKa = 11.84EE93 pKa = 3.89TGSASLALLNTYY105 pKa = 8.71AAKK108 pKa = 10.25IKK110 pKa = 10.29SYY112 pKa = 10.92CDD114 pKa = 3.08TGDD117 pKa = 3.51VFCASGDD124 pKa = 3.41NDD126 pKa = 3.87TAHH129 pKa = 6.03SQEE132 pKa = 4.02VPTWGTHH139 pKa = 4.22AVTFITSLSTT149 pKa = 3.33

Molecular weight: 15.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A559M180|A0A559M180_9HELO Uncharacterized protein (Fragment) OS=Lachnellula willkommii OX=215461 GN=LAWI1_G006382 PE=4 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84SARR5 pKa = 11.84RR6 pKa = 11.84LLLPGVGLGRR16 pKa = 11.84RR17 pKa = 11.84VGVSWLLFRR26 pKa = 11.84GMLRR30 pKa = 11.84RR31 pKa = 11.84MIRR34 pKa = 11.84GLRR37 pKa = 11.84LMFTRR42 pKa = 11.84RR43 pKa = 11.84PQRR46 pKa = 11.84PIPHH50 pKa = 6.5QDD52 pKa = 3.12QLSGLARR59 pKa = 11.84FPP61 pKa = 4.19

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8978

0

8978

4323907

19

4807

481.6

53.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.458 ± 0.021

1.117 ± 0.009

5.578 ± 0.016

6.286 ± 0.024

3.85 ± 0.016

7.077 ± 0.024

2.261 ± 0.01

5.196 ± 0.017

5.293 ± 0.024

8.885 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.189 ± 0.011

3.862 ± 0.012

5.814 ± 0.025

3.859 ± 0.016

5.612 ± 0.023

8.308 ± 0.03

6.024 ± 0.017

6.108 ± 0.017

1.421 ± 0.009

2.804 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski