Tsuneonella aeria

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Tsuneonella

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2648 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A6I4TC93|A0A6I4TC93_9SPHN LPS export ABC transporter permease LptG OS=Tsuneonella aeria OX=1837929 GN=lptG PE=4 SV=1
MM1 pKa = 7.2FLSTRR6 pKa = 11.84GIVGALLAASAMAATPAFAQDD27 pKa = 3.55ASPGLGAFTVSGSATAVTDD46 pKa = 3.31YY47 pKa = 10.88RR48 pKa = 11.84FRR50 pKa = 11.84GVSRR54 pKa = 11.84SGGDD58 pKa = 3.29PAIQGGVTVAHH69 pKa = 7.28DD70 pKa = 3.42SGFYY74 pKa = 10.27AGTWASSIDD83 pKa = 4.02DD84 pKa = 4.76GGTDD88 pKa = 3.54LYY90 pKa = 11.49GDD92 pKa = 3.82VEE94 pKa = 4.92LDD96 pKa = 3.95VFGGWSGGVAEE107 pKa = 5.08GVGIDD112 pKa = 4.27VGLLYY117 pKa = 10.98YY118 pKa = 10.43AFPTNGPGVDD128 pKa = 3.07AGFFEE133 pKa = 5.5PYY135 pKa = 9.5ATVTGDD141 pKa = 4.62LGPLSARR148 pKa = 11.84AGVNYY153 pKa = 10.21AWSQDD158 pKa = 3.38ALGGGDD164 pKa = 3.47NLYY167 pKa = 10.13IHH169 pKa = 7.04TEE171 pKa = 3.96LSSGIPTTPLTVTARR186 pKa = 11.84LGYY189 pKa = 9.15TDD191 pKa = 5.39GPLAPPLLAGTGDD204 pKa = 3.22RR205 pKa = 11.84TGFDD209 pKa = 2.95WSLGAKK215 pKa = 8.68ATVLGGLTLGAAYY228 pKa = 10.12VGSEE232 pKa = 4.34GPGIDD237 pKa = 4.19GFTDD241 pKa = 3.34DD242 pKa = 4.23TVVFSLGASFF252 pKa = 5.21

Molecular weight:
25.16 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A6I4TDF2|A0A6I4TDF2_9SPHN DNA-directed RNA polymerase subunit beta OS=Tsuneonella aeria OX=1837929 GN=rpoB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.89GFFARR21 pKa = 11.84KK22 pKa = 7.42ATPGGRR28 pKa = 11.84KK29 pKa = 8.0VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.81KK41 pKa = 10.46LCAA44 pKa = 3.96

Molecular weight:
5.11 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2648

0

2648

832301

33

3692

314.3

33.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.951 ± 0.076

0.801 ± 0.015

6.052 ± 0.04

5.684 ± 0.047

3.477 ± 0.035

9.073 ± 0.043

1.961 ± 0.025

4.727 ± 0.026

2.659 ± 0.035

9.707 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.452 ± 0.024

2.386 ± 0.03

5.438 ± 0.029

3.006 ± 0.024

7.647 ± 0.052

4.986 ± 0.033

5.165 ± 0.047

7.305 ± 0.031

1.433 ± 0.021

2.091 ± 0.023

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski