Tsuneonella aeria
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2648 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I4TC93|A0A6I4TC93_9SPHN LPS export ABC transporter permease LptG OS=Tsuneonella aeria OX=1837929 GN=lptG PE=4 SV=1
MM1 pKa = 7.2 FLSTRR6 pKa = 11.84 GIVGALLAASAMAATPAFAQDD27 pKa = 3.55 ASPGLGAFTVSGSATAVTDD46 pKa = 3.31 YY47 pKa = 10.88 RR48 pKa = 11.84 FRR50 pKa = 11.84 GVSRR54 pKa = 11.84 SGGDD58 pKa = 3.29 PAIQGGVTVAHH69 pKa = 7.28 DD70 pKa = 3.42 SGFYY74 pKa = 10.27 AGTWASSIDD83 pKa = 4.02 DD84 pKa = 4.76 GGTDD88 pKa = 3.54 LYY90 pKa = 11.49 GDD92 pKa = 3.82 VEE94 pKa = 4.92 LDD96 pKa = 3.95 VFGGWSGGVAEE107 pKa = 5.08 GVGIDD112 pKa = 4.27 VGLLYY117 pKa = 10.98 YY118 pKa = 10.43 AFPTNGPGVDD128 pKa = 3.07 AGFFEE133 pKa = 5.5 PYY135 pKa = 9.5 ATVTGDD141 pKa = 4.62 LGPLSARR148 pKa = 11.84 AGVNYY153 pKa = 10.21 AWSQDD158 pKa = 3.38 ALGGGDD164 pKa = 3.47 NLYY167 pKa = 10.13 IHH169 pKa = 7.04 TEE171 pKa = 3.96 LSSGIPTTPLTVTARR186 pKa = 11.84 LGYY189 pKa = 9.15 TDD191 pKa = 5.39 GPLAPPLLAGTGDD204 pKa = 3.22 RR205 pKa = 11.84 TGFDD209 pKa = 2.95 WSLGAKK215 pKa = 8.68 ATVLGGLTLGAAYY228 pKa = 10.12 VGSEE232 pKa = 4.34 GPGIDD237 pKa = 4.19 GFTDD241 pKa = 3.34 DD242 pKa = 4.23 TVVFSLGASFF252 pKa = 5.21
Molecular weight: 25.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.687
IPC2_protein 3.783
IPC_protein 3.795
Toseland 3.554
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.783
Rodwell 3.617
Grimsley 3.465
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.228
Thurlkill 3.63
EMBOSS 3.795
Sillero 3.923
Patrickios 1.036
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A6I4TDF2|A0A6I4TDF2_9SPHN DNA-directed RNA polymerase subunit beta OS=Tsuneonella aeria OX=1837929 GN=rpoB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.89 GFFARR21 pKa = 11.84 KK22 pKa = 7.42 ATPGGRR28 pKa = 11.84 KK29 pKa = 8.0 VLRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.81 KK41 pKa = 10.46 LCAA44 pKa = 3.96
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2648
0
2648
832301
33
3692
314.3
33.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.951 ± 0.076
0.801 ± 0.015
6.052 ± 0.04
5.684 ± 0.047
3.477 ± 0.035
9.073 ± 0.043
1.961 ± 0.025
4.727 ± 0.026
2.659 ± 0.035
9.707 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.452 ± 0.024
2.386 ± 0.03
5.438 ± 0.029
3.006 ± 0.024
7.647 ± 0.052
4.986 ± 0.033
5.165 ± 0.047
7.305 ± 0.031
1.433 ± 0.021
2.091 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here