Staphylococcus virus SAP26
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E0Y3R5|E0Y3R5_9CAUD Uncharacterized protein OS=Staphylococcus virus SAP26 OX=872294 PE=4 SV=1
MM1 pKa = 7.41 YY2 pKa = 10.23 EE3 pKa = 3.56 LGKK6 pKa = 10.79 YY7 pKa = 7.28 VTEE10 pKa = 3.84 QVYY13 pKa = 10.94 IMMTANDD20 pKa = 4.14 DD21 pKa = 3.74 VEE23 pKa = 4.99 APSDD27 pKa = 3.68 FAKK30 pKa = 10.93 LSDD33 pKa = 3.64 QSDD36 pKa = 3.47 LMRR39 pKa = 11.84 AEE41 pKa = 4.08 VSEE44 pKa = 4.21
Molecular weight: 5.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.126
IPC2_protein 3.923
IPC_protein 3.77
Toseland 3.592
ProMoST 3.884
Dawson 3.757
Bjellqvist 4.037
Wikipedia 3.706
Rodwell 3.617
Grimsley 3.516
Solomon 3.719
Lehninger 3.681
Nozaki 3.91
DTASelect 4.05
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.884
Patrickios 3.198
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.843
Protein with the highest isoelectric point:
>tr|E0Y3Q0|E0Y3Q0_9CAUD Uncharacterized protein OS=Staphylococcus virus SAP26 OX=872294 PE=4 SV=1
MM1 pKa = 7.32 NKK3 pKa = 9.57 ILIRR7 pKa = 11.84 FAINYY12 pKa = 8.84 IKK14 pKa = 10.44 YY15 pKa = 8.01 QQKK18 pKa = 8.41 QLRR21 pKa = 11.84 EE22 pKa = 3.59 KK23 pKa = 9.95 EE24 pKa = 3.86 ARR26 pKa = 11.84 IKK28 pKa = 10.67 YY29 pKa = 10.51 LEE31 pKa = 4.46 GFLKK35 pKa = 10.83 GKK37 pKa = 10.62 GYY39 pKa = 11.17
Molecular weight: 4.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.173
IPC2_protein 9.721
IPC_protein 9.765
Toseland 10.248
ProMoST 9.926
Dawson 10.423
Bjellqvist 10.072
Wikipedia 10.584
Rodwell 11.008
Grimsley 10.496
Solomon 10.452
Lehninger 10.423
Nozaki 10.204
DTASelect 10.072
Thurlkill 10.277
EMBOSS 10.643
Sillero 10.335
Patrickios 10.774
IPC_peptide 10.452
IPC2_peptide 8.639
IPC2.peptide.svr19 8.661
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
63
0
63
12606
39
1155
200.1
22.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.68 ± 0.496
0.42 ± 0.088
6.283 ± 0.383
7.766 ± 0.621
4.292 ± 0.217
5.458 ± 0.267
1.618 ± 0.191
7.623 ± 0.361
9.345 ± 0.376
7.147 ± 0.261
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.562 ± 0.149
7.139 ± 0.275
2.808 ± 0.222
3.966 ± 0.218
4.22 ± 0.294
5.672 ± 0.264
6.045 ± 0.354
6.291 ± 0.345
1.15 ± 0.191
4.514 ± 0.377
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here