Amino acid dipepetide frequency for Staphylococcus virus SAP26

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.349AlaAla: 1.349 ± 0.472
0.397AlaCys: 0.397 ± 0.187
3.094AlaAsp: 3.094 ± 0.453
3.729AlaGlu: 3.729 ± 0.672
2.777AlaPhe: 2.777 ± 0.492
2.856AlaGly: 2.856 ± 0.511
0.793AlaHis: 0.793 ± 0.226
5.395AlaIle: 5.395 ± 0.906
5.553AlaLys: 5.553 ± 0.695
4.125AlaLeu: 4.125 ± 0.642
1.428AlaMet: 1.428 ± 0.369
3.887AlaAsn: 3.887 ± 0.478
1.428AlaPro: 1.428 ± 0.295
2.618AlaGln: 2.618 ± 0.344
3.015AlaArg: 3.015 ± 0.51
2.935AlaSer: 2.935 ± 0.634
3.967AlaThr: 3.967 ± 0.604
4.284AlaVal: 4.284 ± 0.678
0.714AlaTrp: 0.714 ± 0.281
2.459AlaTyr: 2.459 ± 0.411
0.0AlaXaa: 0.0 ± 0.0
Cys
0.238CysAla: 0.238 ± 0.128
0.0CysCys: 0.0 ± 0.0
0.159CysAsp: 0.159 ± 0.107
0.397CysGlu: 0.397 ± 0.188
0.159CysPhe: 0.159 ± 0.121
0.317CysGly: 0.317 ± 0.155
0.0CysHis: 0.0 ± 0.0
0.397CysIle: 0.397 ± 0.264
0.476CysLys: 0.476 ± 0.156
0.079CysLeu: 0.079 ± 0.071
0.0CysMet: 0.0 ± 0.0
0.159CysAsn: 0.159 ± 0.11
0.238CysPro: 0.238 ± 0.123
0.238CysGln: 0.238 ± 0.112
0.397CysArg: 0.397 ± 0.211
0.397CysSer: 0.397 ± 0.222
0.238CysThr: 0.238 ± 0.131
0.079CysVal: 0.079 ± 0.079
0.079CysTrp: 0.079 ± 0.08
0.159CysTyr: 0.159 ± 0.103
0.0CysXaa: 0.0 ± 0.0
Asp
3.332AspAla: 3.332 ± 0.482
0.159AspCys: 0.159 ± 0.113
4.284AspAsp: 4.284 ± 0.802
5.315AspGlu: 5.315 ± 0.748
4.046AspPhe: 4.046 ± 0.626
4.205AspGly: 4.205 ± 0.617
0.476AspHis: 0.476 ± 0.232
5.474AspIle: 5.474 ± 0.692
5.474AspLys: 5.474 ± 0.779
4.284AspLeu: 4.284 ± 0.507
1.587AspMet: 1.587 ± 0.278
4.205AspAsn: 4.205 ± 0.569
1.19AspPro: 1.19 ± 0.315
1.349AspGln: 1.349 ± 0.383
2.38AspArg: 2.38 ± 0.387
4.205AspSer: 4.205 ± 0.478
3.491AspThr: 3.491 ± 0.547
4.443AspVal: 4.443 ± 0.574
0.555AspTrp: 0.555 ± 0.165
2.38AspTyr: 2.38 ± 0.409
0.0AspXaa: 0.0 ± 0.0
Glu
5.633GluAla: 5.633 ± 0.789
0.555GluCys: 0.555 ± 0.176
3.253GluAsp: 3.253 ± 0.569
5.871GluGlu: 5.871 ± 0.819
3.173GluPhe: 3.173 ± 0.508
3.808GluGly: 3.808 ± 0.482
1.269GluHis: 1.269 ± 0.365
6.029GluIle: 6.029 ± 0.971
5.712GluLys: 5.712 ± 0.74
6.902GluLeu: 6.902 ± 0.964
2.063GluMet: 2.063 ± 0.371
5.077GluAsn: 5.077 ± 0.627
1.983GluPro: 1.983 ± 0.373
4.363GluGln: 4.363 ± 0.667
3.491GluArg: 3.491 ± 0.624
3.887GluSer: 3.887 ± 0.594
3.332GluThr: 3.332 ± 0.423
5.315GluVal: 5.315 ± 0.669
0.952GluTrp: 0.952 ± 0.283
4.998GluTyr: 4.998 ± 0.72
0.0GluXaa: 0.0 ± 0.0
Phe
2.38PheAla: 2.38 ± 0.387
0.238PheCys: 0.238 ± 0.116
3.57PheAsp: 3.57 ± 0.464
3.967PheGlu: 3.967 ± 0.538
1.031PhePhe: 1.031 ± 0.272
2.618PheGly: 2.618 ± 0.422
0.714PheHis: 0.714 ± 0.264
2.459PheIle: 2.459 ± 0.403
4.363PheLys: 4.363 ± 0.639
2.935PheLeu: 2.935 ± 0.444
1.428PheMet: 1.428 ± 0.401
3.649PheAsn: 3.649 ± 0.519
0.714PhePro: 0.714 ± 0.285
1.428PheGln: 1.428 ± 0.437
1.428PheArg: 1.428 ± 0.323
3.015PheSer: 3.015 ± 0.534
2.777PheThr: 2.777 ± 0.443
2.459PheVal: 2.459 ± 0.531
0.238PheTrp: 0.238 ± 0.13
1.507PheTyr: 1.507 ± 0.272
0.0PheXaa: 0.0 ± 0.0
Gly
2.618GlyAla: 2.618 ± 0.407
0.317GlyCys: 0.317 ± 0.16
3.729GlyAsp: 3.729 ± 0.525
3.411GlyGlu: 3.411 ± 0.537
2.38GlyPhe: 2.38 ± 0.471
3.015GlyGly: 3.015 ± 0.502
1.349GlyHis: 1.349 ± 0.39
4.046GlyIle: 4.046 ± 0.599
4.76GlyLys: 4.76 ± 0.708
4.919GlyLeu: 4.919 ± 0.821
1.507GlyMet: 1.507 ± 0.34
3.173GlyAsn: 3.173 ± 0.474
0.635GlyPro: 0.635 ± 0.268
1.745GlyGln: 1.745 ± 0.305
2.301GlyArg: 2.301 ± 0.404
2.539GlySer: 2.539 ± 0.5
4.046GlyThr: 4.046 ± 0.571
4.284GlyVal: 4.284 ± 0.584
0.873GlyTrp: 0.873 ± 0.301
2.856GlyTyr: 2.856 ± 0.373
0.0GlyXaa: 0.0 ± 0.0
His
1.349HisAla: 1.349 ± 0.399
0.0HisCys: 0.0 ± 0.0
1.19HisAsp: 1.19 ± 0.254
1.111HisGlu: 1.111 ± 0.359
0.952HisPhe: 0.952 ± 0.296
0.873HisGly: 0.873 ± 0.261
0.397HisHis: 0.397 ± 0.184
1.19HisIle: 1.19 ± 0.313
0.793HisLys: 0.793 ± 0.238
1.111HisLeu: 1.111 ± 0.261
0.317HisMet: 0.317 ± 0.132
0.952HisAsn: 0.952 ± 0.262
0.714HisPro: 0.714 ± 0.226
0.793HisGln: 0.793 ± 0.23
0.159HisArg: 0.159 ± 0.103
1.349HisSer: 1.349 ± 0.271
0.952HisThr: 0.952 ± 0.266
1.031HisVal: 1.031 ± 0.295
0.079HisTrp: 0.079 ± 0.079
0.873HisTyr: 0.873 ± 0.369
0.0HisXaa: 0.0 ± 0.0
Ile
4.839IleAla: 4.839 ± 0.718
0.238IleCys: 0.238 ± 0.119
6.029IleAsp: 6.029 ± 0.643
6.902IleGlu: 6.902 ± 0.722
3.015IlePhe: 3.015 ± 0.485
4.046IleGly: 4.046 ± 0.561
0.952IleHis: 0.952 ± 0.198
4.601IleIle: 4.601 ± 0.744
6.902IleLys: 6.902 ± 0.676
4.76IleLeu: 4.76 ± 0.584
1.825IleMet: 1.825 ± 0.354
5.395IleAsn: 5.395 ± 0.652
2.221IlePro: 2.221 ± 0.399
2.142IleGln: 2.142 ± 0.472
3.649IleArg: 3.649 ± 0.596
3.967IleSer: 3.967 ± 0.638
5.474IleThr: 5.474 ± 0.676
4.284IleVal: 4.284 ± 0.57
1.587IleTrp: 1.587 ± 0.624
3.411IleTyr: 3.411 ± 0.522
0.0IleXaa: 0.0 ± 0.0
Lys
6.188LysAla: 6.188 ± 0.813
0.238LysCys: 0.238 ± 0.143
5.95LysAsp: 5.95 ± 0.805
7.854LysGlu: 7.854 ± 1.035
3.332LysPhe: 3.332 ± 0.592
5.157LysGly: 5.157 ± 0.631
1.666LysHis: 1.666 ± 0.397
5.871LysIle: 5.871 ± 0.571
8.568LysLys: 8.568 ± 0.83
6.267LysLeu: 6.267 ± 0.619
2.618LysMet: 2.618 ± 0.564
4.919LysAsn: 4.919 ± 0.639
3.649LysPro: 3.649 ± 0.602
5.077LysGln: 5.077 ± 0.716
4.522LysArg: 4.522 ± 0.711
5.712LysSer: 5.712 ± 0.696
4.839LysThr: 4.839 ± 0.598
5.95LysVal: 5.95 ± 0.801
0.555LysTrp: 0.555 ± 0.163
4.522LysTyr: 4.522 ± 0.858
0.0LysXaa: 0.0 ± 0.0
Leu
3.173LeuAla: 3.173 ± 0.544
0.317LeuCys: 0.317 ± 0.247
4.205LeuAsp: 4.205 ± 0.606
5.95LeuGlu: 5.95 ± 0.691
3.015LeuPhe: 3.015 ± 0.568
3.57LeuGly: 3.57 ± 0.497
0.952LeuHis: 0.952 ± 0.28
4.522LeuIle: 4.522 ± 0.618
7.299LeuLys: 7.299 ± 0.677
5.157LeuLeu: 5.157 ± 0.634
1.587LeuMet: 1.587 ± 0.309
5.633LeuAsn: 5.633 ± 0.546
2.221LeuPro: 2.221 ± 0.34
3.173LeuGln: 3.173 ± 0.593
3.173LeuArg: 3.173 ± 0.503
4.76LeuSer: 4.76 ± 0.587
4.919LeuThr: 4.919 ± 0.61
4.522LeuVal: 4.522 ± 0.614
0.555LeuTrp: 0.555 ± 0.248
2.777LeuTyr: 2.777 ± 0.528
0.0LeuXaa: 0.0 ± 0.0
Met
1.428MetAla: 1.428 ± 0.297
0.079MetCys: 0.079 ± 0.079
1.19MetAsp: 1.19 ± 0.275
1.745MetGlu: 1.745 ± 0.413
0.873MetPhe: 0.873 ± 0.289
0.873MetGly: 0.873 ± 0.266
0.317MetHis: 0.317 ± 0.186
1.587MetIle: 1.587 ± 0.371
2.539MetLys: 2.539 ± 0.444
2.142MetLeu: 2.142 ± 0.299
0.873MetMet: 0.873 ± 0.243
2.063MetAsn: 2.063 ± 0.387
0.952MetPro: 0.952 ± 0.244
1.269MetGln: 1.269 ± 0.34
0.873MetArg: 0.873 ± 0.292
1.428MetSer: 1.428 ± 0.371
2.301MetThr: 2.301 ± 0.45
1.349MetVal: 1.349 ± 0.258
0.635MetTrp: 0.635 ± 0.227
1.111MetTyr: 1.111 ± 0.269
0.0MetXaa: 0.0 ± 0.0
Asn
5.712AsnAla: 5.712 ± 0.7
0.238AsnCys: 0.238 ± 0.144
4.601AsnAsp: 4.601 ± 0.623
5.157AsnGlu: 5.157 ± 0.612
3.015AsnPhe: 3.015 ± 0.599
5.157AsnGly: 5.157 ± 0.652
0.873AsnHis: 0.873 ± 0.271
4.601AsnIle: 4.601 ± 0.518
7.299AsnLys: 7.299 ± 0.68
4.284AsnLeu: 4.284 ± 0.62
1.666AsnMet: 1.666 ± 0.327
5.871AsnAsn: 5.871 ± 1.021
2.935AsnPro: 2.935 ± 0.583
2.38AsnGln: 2.38 ± 0.424
2.935AsnArg: 2.935 ± 0.351
3.491AsnSer: 3.491 ± 0.527
3.57AsnThr: 3.57 ± 0.493
4.205AsnVal: 4.205 ± 0.637
0.635AsnTrp: 0.635 ± 0.214
2.777AsnTyr: 2.777 ± 0.566
0.0AsnXaa: 0.0 ± 0.0
Pro
1.111ProAla: 1.111 ± 0.344
0.238ProCys: 0.238 ± 0.125
1.507ProAsp: 1.507 ± 0.311
1.983ProGlu: 1.983 ± 0.305
1.111ProPhe: 1.111 ± 0.354
1.983ProGly: 1.983 ± 0.441
0.555ProHis: 0.555 ± 0.194
1.904ProIle: 1.904 ± 0.346
3.173ProLys: 3.173 ± 0.666
1.19ProLeu: 1.19 ± 0.272
0.873ProMet: 0.873 ± 0.235
2.142ProAsn: 2.142 ± 0.426
0.555ProPro: 0.555 ± 0.283
1.269ProGln: 1.269 ± 0.347
1.19ProArg: 1.19 ± 0.289
1.507ProSer: 1.507 ± 0.314
2.221ProThr: 2.221 ± 0.409
2.063ProVal: 2.063 ± 0.408
0.079ProTrp: 0.079 ± 0.076
1.428ProTyr: 1.428 ± 0.339
0.0ProXaa: 0.0 ± 0.0
Gln
2.539GlnAla: 2.539 ± 0.451
0.397GlnCys: 0.397 ± 0.195
2.38GlnAsp: 2.38 ± 0.474
2.618GlnGlu: 2.618 ± 0.562
1.428GlnPhe: 1.428 ± 0.328
1.904GlnGly: 1.904 ± 0.303
0.714GlnHis: 0.714 ± 0.173
3.015GlnIle: 3.015 ± 0.46
3.808GlnLys: 3.808 ± 0.363
3.332GlnLeu: 3.332 ± 0.629
1.349GlnMet: 1.349 ± 0.29
2.935GlnAsn: 2.935 ± 0.7
1.269GlnPro: 1.269 ± 0.339
1.745GlnGln: 1.745 ± 0.544
1.904GlnArg: 1.904 ± 0.415
2.459GlnSer: 2.459 ± 0.367
1.983GlnThr: 1.983 ± 0.399
1.825GlnVal: 1.825 ± 0.351
0.317GlnTrp: 0.317 ± 0.153
1.745GlnTyr: 1.745 ± 0.46
0.0GlnXaa: 0.0 ± 0.0
Arg
1.507ArgAla: 1.507 ± 0.355
0.159ArgCys: 0.159 ± 0.105
2.301ArgAsp: 2.301 ± 0.501
3.173ArgGlu: 3.173 ± 0.546
1.904ArgPhe: 1.904 ± 0.457
1.904ArgGly: 1.904 ± 0.364
1.269ArgHis: 1.269 ± 0.328
3.887ArgIle: 3.887 ± 0.513
4.76ArgLys: 4.76 ± 0.699
3.808ArgLeu: 3.808 ± 0.574
1.19ArgMet: 1.19 ± 0.324
3.094ArgAsn: 3.094 ± 0.498
1.031ArgPro: 1.031 ± 0.273
1.507ArgGln: 1.507 ± 0.35
1.904ArgArg: 1.904 ± 0.436
1.745ArgSer: 1.745 ± 0.296
1.983ArgThr: 1.983 ± 0.306
2.38ArgVal: 2.38 ± 0.53
0.555ArgTrp: 0.555 ± 0.24
2.142ArgTyr: 2.142 ± 0.473
0.0ArgXaa: 0.0 ± 0.0
Ser
3.967SerAla: 3.967 ± 0.699
0.159SerCys: 0.159 ± 0.094
4.522SerAsp: 4.522 ± 0.689
3.649SerGlu: 3.649 ± 0.611
2.935SerPhe: 2.935 ± 0.566
3.253SerGly: 3.253 ± 0.649
1.349SerHis: 1.349 ± 0.307
5.315SerIle: 5.315 ± 0.561
5.236SerLys: 5.236 ± 0.587
3.729SerLeu: 3.729 ± 0.471
1.507SerMet: 1.507 ± 0.318
4.125SerAsn: 4.125 ± 0.505
0.952SerPro: 0.952 ± 0.333
1.587SerGln: 1.587 ± 0.313
2.221SerArg: 2.221 ± 0.313
3.173SerSer: 3.173 ± 0.428
3.491SerThr: 3.491 ± 0.408
2.777SerVal: 2.777 ± 0.63
0.317SerTrp: 0.317 ± 0.197
2.459SerTyr: 2.459 ± 0.44
0.0SerXaa: 0.0 ± 0.0
Thr
3.57ThrAla: 3.57 ± 0.573
0.0ThrCys: 0.0 ± 0.0
3.887ThrAsp: 3.887 ± 0.707
4.601ThrGlu: 4.601 ± 0.573
2.38ThrPhe: 2.38 ± 0.449
3.57ThrGly: 3.57 ± 0.525
0.873ThrHis: 0.873 ± 0.209
6.267ThrIle: 6.267 ± 1.204
5.395ThrLys: 5.395 ± 0.631
3.967ThrLeu: 3.967 ± 0.456
1.111ThrMet: 1.111 ± 0.375
4.443ThrAsn: 4.443 ± 0.695
1.745ThrPro: 1.745 ± 0.351
2.935ThrGln: 2.935 ± 0.516
2.142ThrArg: 2.142 ± 0.37
3.094ThrSer: 3.094 ± 0.718
3.57ThrThr: 3.57 ± 0.61
3.887ThrVal: 3.887 ± 0.545
0.635ThrTrp: 0.635 ± 0.204
2.38ThrTyr: 2.38 ± 0.453
0.0ThrXaa: 0.0 ± 0.0
Val
3.094ValAla: 3.094 ± 0.887
0.238ValCys: 0.238 ± 0.123
4.601ValAsp: 4.601 ± 0.583
5.633ValGlu: 5.633 ± 0.808
2.618ValPhe: 2.618 ± 0.538
2.618ValGly: 2.618 ± 0.512
0.635ValHis: 0.635 ± 0.227
5.633ValIle: 5.633 ± 0.548
5.791ValLys: 5.791 ± 0.564
4.839ValLeu: 4.839 ± 0.704
1.349ValMet: 1.349 ± 0.311
4.998ValAsn: 4.998 ± 0.665
2.142ValPro: 2.142 ± 0.513
1.745ValGln: 1.745 ± 0.362
2.063ValArg: 2.063 ± 0.439
3.887ValSer: 3.887 ± 0.677
3.332ValThr: 3.332 ± 0.445
3.967ValVal: 3.967 ± 0.543
1.031ValTrp: 1.031 ± 0.369
2.697ValTyr: 2.697 ± 0.51
0.0ValXaa: 0.0 ± 0.0
Trp
0.873TrpAla: 0.873 ± 0.335
0.159TrpCys: 0.159 ± 0.114
0.238TrpAsp: 0.238 ± 0.13
0.635TrpGlu: 0.635 ± 0.189
0.476TrpPhe: 0.476 ± 0.194
0.714TrpGly: 0.714 ± 0.371
0.159TrpHis: 0.159 ± 0.104
0.714TrpIle: 0.714 ± 0.245
0.793TrpLys: 0.793 ± 0.243
0.476TrpLeu: 0.476 ± 0.163
0.238TrpMet: 0.238 ± 0.134
1.745TrpAsn: 1.745 ± 0.979
0.159TrpPro: 0.159 ± 0.109
0.476TrpGln: 0.476 ± 0.211
0.317TrpArg: 0.317 ± 0.141
0.873TrpSer: 0.873 ± 0.294
1.031TrpThr: 1.031 ± 0.302
0.555TrpVal: 0.555 ± 0.235
0.079TrpTrp: 0.079 ± 0.089
0.793TrpTyr: 0.793 ± 0.275
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.904TyrAla: 1.904 ± 0.378
0.079TyrCys: 0.079 ± 0.079
2.142TyrAsp: 2.142 ± 0.342
3.967TyrGlu: 3.967 ± 0.623
2.301TyrPhe: 2.301 ± 0.511
2.142TyrGly: 2.142 ± 0.493
0.873TyrHis: 0.873 ± 0.28
3.332TyrIle: 3.332 ± 0.534
4.76TyrLys: 4.76 ± 0.627
3.173TyrLeu: 3.173 ± 0.517
1.031TyrMet: 1.031 ± 0.278
2.935TyrAsn: 2.935 ± 0.431
1.349TyrPro: 1.349 ± 0.362
1.825TyrGln: 1.825 ± 0.424
2.142TyrArg: 2.142 ± 0.467
2.301TyrSer: 2.301 ± 0.409
2.935TyrThr: 2.935 ± 0.446
3.253TyrVal: 3.253 ± 0.492
1.031TyrTrp: 1.031 ± 0.288
1.666TyrTyr: 1.666 ± 0.32
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (12606 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski