Sphingobium ummariense RL-3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; Sphingobium ummariense

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4462 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0J014|T0J014_9SPHN Nitrite reductase OS=Sphingobium ummariense RL-3 OX=1346791 GN=M529_15220 PE=4 SV=1
MM1 pKa = 7.66AKK3 pKa = 10.39LIVVNRR9 pKa = 11.84AGEE12 pKa = 4.07EE13 pKa = 3.89QAVEE17 pKa = 4.06GDD19 pKa = 3.36NGLSVMEE26 pKa = 5.01IIRR29 pKa = 11.84DD30 pKa = 3.56NGFDD34 pKa = 4.08EE35 pKa = 5.76LLALCGGCCSCATCHH50 pKa = 6.48VYY52 pKa = 10.52VDD54 pKa = 3.85PAFAGALSPMSEE66 pKa = 4.75DD67 pKa = 4.05EE68 pKa = 4.58NDD70 pKa = 4.2LLDD73 pKa = 5.43SSDD76 pKa = 3.43QRR78 pKa = 11.84NEE80 pKa = 3.69TSRR83 pKa = 11.84LSCQLVFSDD92 pKa = 4.82ALDD95 pKa = 3.8GLRR98 pKa = 11.84VTIAPEE104 pKa = 3.78DD105 pKa = 3.45

Molecular weight: 11.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0J8A0|T0J8A0_9SPHN ArsR family transcriptional regulator OS=Sphingobium ummariense RL-3 OX=1346791 GN=M529_00735 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.06SLSAA44 pKa = 3.93

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4462

0

4462

1346903

29

1937

301.9

32.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.227 ± 0.048

0.778 ± 0.011

6.006 ± 0.028

5.367 ± 0.035

3.52 ± 0.02

8.867 ± 0.034

2.005 ± 0.02

4.981 ± 0.025

2.979 ± 0.029

9.991 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.495 ± 0.022

2.508 ± 0.03

5.369 ± 0.031

3.213 ± 0.022

7.562 ± 0.042

5.164 ± 0.027

5.129 ± 0.026

7.072 ± 0.033

1.466 ± 0.017

2.299 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski