Yoonia sediminilitoris
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4606 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T6K8K1|A0A2T6K8K1_9RHOB Amino acid/amide ABC transporter substrate-binding protein (HAAT family) OS=Yoonia sediminilitoris OX=1286148 GN=C8N45_11527 PE=4 SV=1
MM1 pKa = 7.58 NYY3 pKa = 10.2 AISRR7 pKa = 11.84 IAAFAALLMIAAPASAATYY26 pKa = 10.14 SYY28 pKa = 11.68 DD29 pKa = 3.25 FTTLNSGQTTYY40 pKa = 11.41 SSGLAVEE47 pKa = 4.64 SDD49 pKa = 3.45 GLTVEE54 pKa = 4.59 VTAGRR59 pKa = 11.84 YY60 pKa = 8.86 DD61 pKa = 3.45 SYY63 pKa = 11.28 MGLDD67 pKa = 3.52 NDD69 pKa = 4.72 QIADD73 pKa = 3.8 TDD75 pKa = 4.26 CADD78 pKa = 5.66 GGCQWYY84 pKa = 9.95 DD85 pKa = 3.27 NSVGEE90 pKa = 4.17 LLGYY94 pKa = 9.81 RR95 pKa = 11.84 AVAITSDD102 pKa = 3.39 GLGIAGFFDD111 pKa = 4.55 GSDD114 pKa = 3.11 IDD116 pKa = 4.9 GDD118 pKa = 4.01 FGNDD122 pKa = 3.64 LITFTFDD129 pKa = 2.51 RR130 pKa = 11.84 VVDD133 pKa = 4.48 FTRR136 pKa = 11.84 VFFTGVEE143 pKa = 4.26 SFGYY147 pKa = 10.51 AQDD150 pKa = 3.59 QFDD153 pKa = 3.94 VFVDD157 pKa = 4.64 GVLLRR162 pKa = 11.84 EE163 pKa = 3.99 EE164 pKa = 4.26 VLIGEE169 pKa = 4.34 NNPYY173 pKa = 10.99 DD174 pKa = 3.72 LTGFSAIGRR183 pKa = 11.84 SISFGADD190 pKa = 2.4 SGYY193 pKa = 11.03 DD194 pKa = 3.22 NFRR197 pKa = 11.84 IGGLEE202 pKa = 3.93 VEE204 pKa = 4.92 LAAVPLPAGGLLLLSGLFGLALARR228 pKa = 11.84 RR229 pKa = 11.84 RR230 pKa = 11.84 GTAAA234 pKa = 3.56
Molecular weight: 24.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.685
IPC2_protein 3.706
IPC_protein 3.732
Toseland 3.49
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.126
Thurlkill 3.554
EMBOSS 3.694
Sillero 3.846
Patrickios 1.062
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.77
Protein with the highest isoelectric point:
>tr|A0A2T6KG93|A0A2T6KG93_9RHOB Uncharacterized protein OS=Yoonia sediminilitoris OX=1286148 GN=C8N45_106206 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.35 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4606
0
4606
1395618
25
10445
303.0
32.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.864 ± 0.047
0.949 ± 0.013
6.382 ± 0.039
5.368 ± 0.035
3.841 ± 0.029
8.47 ± 0.044
2.062 ± 0.02
5.587 ± 0.028
3.362 ± 0.032
9.745 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.847 ± 0.026
2.931 ± 0.029
4.944 ± 0.032
3.511 ± 0.022
6.161 ± 0.037
5.316 ± 0.032
5.759 ± 0.037
7.238 ± 0.031
1.389 ± 0.019
2.274 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here