Faecalicoccus pleomorphus
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1976 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A380LLL7|A0A380LLL7_9FIRM Polar amino acid transport system permease /substrate-binding protein OS=Faecalicoccus pleomorphus OX=1323 GN=fliY PE=4 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.02 FVKK5 pKa = 10.43 GFILIFSIVSILVFTASVVKK25 pKa = 10.46 QFFSYY30 pKa = 10.62 DD31 pKa = 2.91 ASKK34 pKa = 10.53 PVIVSDD40 pKa = 3.73 VEE42 pKa = 4.39 EE43 pKa = 4.79 IEE45 pKa = 4.4 IPCDD49 pKa = 3.42 YY50 pKa = 10.45 TRR52 pKa = 11.84 EE53 pKa = 3.94 QIMQGLHH60 pKa = 6.9 ASDD63 pKa = 5.01 DD64 pKa = 3.81 QDD66 pKa = 5.21 GDD68 pKa = 3.71 LTDD71 pKa = 3.77 QIVVGSISRR80 pKa = 11.84 FIEE83 pKa = 4.29 KK84 pKa = 10.2 GLCDD88 pKa = 3.18 VTYY91 pKa = 10.26 TVFDD95 pKa = 4.06 SSNQSASLTRR105 pKa = 11.84 RR106 pKa = 11.84 IRR108 pKa = 11.84 FTDD111 pKa = 3.35 YY112 pKa = 10.59 QSPVFQLSAPLVFEE126 pKa = 4.48 EE127 pKa = 5.19 GAMSSMSILDD137 pKa = 4.17 LFTAYY142 pKa = 10.28 DD143 pKa = 3.92 VLDD146 pKa = 3.66 QDD148 pKa = 3.33 ITDD151 pKa = 4.7 NIMQVDD157 pKa = 3.54 SDD159 pKa = 4.28 VNYY162 pKa = 10.45 SSQGRR167 pKa = 11.84 YY168 pKa = 9.68 EE169 pKa = 3.72 ITLEE173 pKa = 4.06 VNNTKK178 pKa = 10.69 GDD180 pKa = 3.69 TATLSLPVYY189 pKa = 9.95 ILPEE193 pKa = 4.31 DD194 pKa = 3.88 SVNCSITLSDD204 pKa = 3.67 YY205 pKa = 11.19 LVYY208 pKa = 11.06 LNVGDD213 pKa = 4.54 GFDD216 pKa = 3.47 PSGYY220 pKa = 9.11 IQSAMDD226 pKa = 4.29 DD227 pKa = 4.05 DD228 pKa = 5.22 GNNLDD233 pKa = 4.0 VSQLQITSNVDD244 pKa = 2.87 TSKK247 pKa = 11.05 AGTYY251 pKa = 7.11 EE252 pKa = 4.01 VCFDD256 pKa = 4.34 PTPLGYY262 pKa = 10.44 SGVVYY267 pKa = 7.22 MTVVVRR273 pKa = 4.78
Molecular weight: 30.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.757
IPC_protein 3.783
Toseland 3.541
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.605
Grimsley 3.452
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.91
Patrickios 0.718
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.809
Protein with the highest isoelectric point:
>tr|A0A380LN09|A0A380LN09_9FIRM Uncharacterized protein OS=Faecalicoccus pleomorphus OX=1323 GN=NCTC11087_01559 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.14 QPSKK9 pKa = 10.05 RR10 pKa = 11.84 KK11 pKa = 8.47 HH12 pKa = 5.31 QKK14 pKa = 6.82 THH16 pKa = 4.76 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.94 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.389
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.31
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.047
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.016
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1976
0
1976
605430
30
2481
306.4
34.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.501 ± 0.049
1.572 ± 0.027
5.832 ± 0.054
6.999 ± 0.048
4.366 ± 0.044
6.243 ± 0.055
2.2 ± 0.034
7.81 ± 0.059
7.27 ± 0.049
9.426 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.073 ± 0.031
4.39 ± 0.037
3.245 ± 0.029
4.366 ± 0.044
3.76 ± 0.044
5.971 ± 0.038
5.301 ± 0.058
6.668 ± 0.048
0.889 ± 0.016
4.117 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here