Salmonella virus KFS-SE2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5TMD9|A0A4Y5TMD9_9CAUD Putative DNA repair helicase OS=Salmonella virus KFS-SE2 OX=2583286 PE=4 SV=1
MM1 pKa = 7.87TITKK5 pKa = 9.75QRR7 pKa = 11.84VEE9 pKa = 4.33EE10 pKa = 4.39IISRR14 pKa = 11.84IEE16 pKa = 4.0MYY18 pKa = 10.88GHH20 pKa = 6.38GAGYY24 pKa = 7.78TAEE27 pKa = 4.2EE28 pKa = 4.85VYY30 pKa = 11.05DD31 pKa = 4.06LAVLALNLSNIAKK44 pKa = 8.26LTRR47 pKa = 11.84YY48 pKa = 9.79DD49 pKa = 4.66LNTGGCDD56 pKa = 4.13SCGQDD61 pKa = 3.47CGADD65 pKa = 3.33MSEE68 pKa = 4.75DD69 pKa = 3.75PDD71 pKa = 4.2GGFVMFDD78 pKa = 3.41DD79 pKa = 4.45VVSLVQFDD87 pKa = 3.49TTAQQFEE94 pKa = 4.58SLAKK98 pKa = 10.4SEE100 pKa = 4.15

Molecular weight: 10.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5TNB0|A0A4Y5TNB0_9CAUD Endolysin OS=Salmonella virus KFS-SE2 OX=2583286 PE=3 SV=1
MM1 pKa = 7.37SKK3 pKa = 10.58CSTIHH8 pKa = 5.29QLVMADD14 pKa = 2.9IRR16 pKa = 11.84EE17 pKa = 4.26SNARR21 pKa = 11.84NKK23 pKa = 10.24KK24 pKa = 9.88SRR26 pKa = 11.84NQKK29 pKa = 8.53NWYY32 pKa = 9.45GGIGFLKK39 pKa = 10.95AMFKK43 pKa = 10.06TSKK46 pKa = 9.29KK47 pKa = 9.07HH48 pKa = 4.04YY49 pKa = 9.5RR50 pKa = 11.84RR51 pKa = 11.84DD52 pKa = 3.54RR53 pKa = 11.84ILRR56 pKa = 11.84RR57 pKa = 11.84LCDD60 pKa = 4.02LNMKK64 pKa = 10.33KK65 pKa = 10.1LVKK68 pKa = 10.45EE69 pKa = 3.85MTKK72 pKa = 10.83

Molecular weight: 8.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

14499

41

828

172.6

19.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.297 ± 0.495

1.586 ± 0.176

6.276 ± 0.28

6.607 ± 0.332

3.524 ± 0.202

7.304 ± 0.293

1.876 ± 0.178

6.614 ± 0.165

6.642 ± 0.316

7.207 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.159 ± 0.197

4.787 ± 0.21

3.297 ± 0.223

3.938 ± 0.295

5.131 ± 0.279

6.338 ± 0.29

5.38 ± 0.356

6.683 ± 0.262

1.586 ± 0.123

3.752 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski