Skermanella aerolata

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Azospirillaceae; Skermanella

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7916 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A512DNG4|A0A512DNG4_9PROT 4Fe-4S ferredoxin OS=Skermanella aerolata OX=393310 GN=SAE02_21710 PE=4 SV=1
MM1 pKa = 7.83AIFNGTAARR10 pKa = 11.84DD11 pKa = 3.77IYY13 pKa = 11.15NGTSSADD20 pKa = 3.31SARR23 pKa = 11.84GNGGDD28 pKa = 4.01DD29 pKa = 3.59TLSGLGGGDD38 pKa = 3.56ALYY41 pKa = 10.96GDD43 pKa = 4.7SGNDD47 pKa = 3.42GLYY50 pKa = 10.62GGTGIDD56 pKa = 3.99RR57 pKa = 11.84IFGGTGVDD65 pKa = 3.64ALFGQDD71 pKa = 5.31GADD74 pKa = 3.37QLYY77 pKa = 10.88GEE79 pKa = 5.46AGNDD83 pKa = 3.32WLQGGAGNDD92 pKa = 3.75YY93 pKa = 11.24LHH95 pKa = 7.01GGSGNDD101 pKa = 3.7SLRR104 pKa = 11.84GDD106 pKa = 5.03DD107 pKa = 6.29GDD109 pKa = 3.98DD110 pKa = 3.64TLNGAVGDD118 pKa = 3.71NDD120 pKa = 3.59LRR122 pKa = 11.84GGAGNDD128 pKa = 3.61TLIHH132 pKa = 6.09SGIGPTYY139 pKa = 10.35SPTVNAFYY147 pKa = 10.78DD148 pKa = 3.92GGSGHH153 pKa = 7.15DD154 pKa = 4.04TLLIDD159 pKa = 3.17YY160 pKa = 8.92WGDD163 pKa = 3.63YY164 pKa = 10.04IQTSTDD170 pKa = 3.67FSPWVEE176 pKa = 3.8ININPDD182 pKa = 2.81ASSTMNYY189 pKa = 10.23SSDD192 pKa = 3.76PLEE195 pKa = 4.4GPYY198 pKa = 10.4EE199 pKa = 4.0IVAAFAGIEE208 pKa = 4.14EE209 pKa = 4.2FRR211 pKa = 11.84LTGEE215 pKa = 4.1TGRR218 pKa = 11.84LSVTARR224 pKa = 11.84TDD226 pKa = 3.26AVIFGNDD233 pKa = 3.2EE234 pKa = 3.81RR235 pKa = 11.84DD236 pKa = 3.53KK237 pKa = 11.8LEE239 pKa = 4.05GRR241 pKa = 11.84QRR243 pKa = 11.84DD244 pKa = 3.31QDD246 pKa = 3.61FTGGGGADD254 pKa = 3.15EE255 pKa = 4.41YY256 pKa = 11.03QFYY259 pKa = 8.92WRR261 pKa = 11.84KK262 pKa = 9.95GFEE265 pKa = 4.29PNHH268 pKa = 5.91DD269 pKa = 3.9TVHH272 pKa = 6.05GFSVAEE278 pKa = 4.17GDD280 pKa = 3.79TIAFGNQSEE289 pKa = 4.41NDD291 pKa = 3.58LDD293 pKa = 4.06EE294 pKa = 5.17PLPLDD299 pKa = 3.35ITAEE303 pKa = 4.21EE304 pKa = 4.73VNGHH308 pKa = 6.07TIYY311 pKa = 10.73TSVEE315 pKa = 3.64IATGQVVHH323 pKa = 6.39TLDD326 pKa = 3.44VDD328 pKa = 4.04AVGLPEE334 pKa = 4.22PQPWYY339 pKa = 10.8YY340 pKa = 10.98LGG342 pKa = 3.91

Molecular weight: 36.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A512DWP1|A0A512DWP1_9PROT Uncharacterized protein OS=Skermanella aerolata OX=393310 GN=SAE02_50290 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.09QPSKK9 pKa = 9.06IVRR12 pKa = 11.84KK13 pKa = 9.03HH14 pKa = 4.06RR15 pKa = 11.84HH16 pKa = 4.08GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.97GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7916

0

7916

2418692

39

3687

305.5

33.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.977 ± 0.037

0.871 ± 0.009

5.847 ± 0.025

5.632 ± 0.025

3.545 ± 0.014

8.72 ± 0.025

2.083 ± 0.014

5.075 ± 0.019

2.986 ± 0.021

10.366 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.012

2.532 ± 0.015

5.369 ± 0.023

3.111 ± 0.018

7.35 ± 0.031

5.433 ± 0.017

5.432 ± 0.022

7.668 ± 0.025

1.324 ± 0.012

2.147 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski