Bacillus phage TP21-L

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lwoffvirus; Bacillus virus TP21

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8R847|B8R847_9CAUD Gp38 OS=Bacillus phage TP21-L OX=565140 PE=4 SV=1
MM1 pKa = 7.51WLSTSLICPSCKK13 pKa = 9.69GHH15 pKa = 7.09LFLMHH20 pKa = 7.09SDD22 pKa = 3.68NEE24 pKa = 4.06IHH26 pKa = 7.09LEE28 pKa = 4.14CDD30 pKa = 3.95TIACDD35 pKa = 3.67YY36 pKa = 11.29SKK38 pKa = 10.59MIDD41 pKa = 3.27IQEE44 pKa = 4.44VIDD47 pKa = 3.84NDD49 pKa = 3.75EE50 pKa = 3.77

Molecular weight: 5.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8R862|B8R862_9CAUD Gp53 OS=Bacillus phage TP21-L OX=565140 PE=4 SV=1
MM1 pKa = 5.85VTIYY5 pKa = 10.67RR6 pKa = 11.84FLFIVGTLLMVGGGTIVLSFPLRR29 pKa = 11.84VISWALGASVLL40 pKa = 4.34

Molecular weight: 4.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

11101

40

1706

198.2

22.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.639 ± 0.474

0.838 ± 0.157

5.639 ± 0.286

8.369 ± 0.342

3.711 ± 0.197

6.81 ± 0.48

1.739 ± 0.19

6.927 ± 0.177

8.882 ± 0.404

7.171 ± 0.18

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.441 ± 0.22

5.801 ± 0.344

2.883 ± 0.254

3.756 ± 0.253

5.162 ± 0.357

5.459 ± 0.292

6.27 ± 0.383

6.369 ± 0.318

1.279 ± 0.123

3.856 ± 0.37
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski