Amino acid dipepetide frequency for Bacillus phage TP21-L

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.45AlaCys: 0.45 ± 0.225
2.703AlaAsp: 2.703 ± 0.546
5.225AlaGlu: 5.225 ± 0.728
2.703AlaPhe: 2.703 ± 0.493
4.505AlaGly: 4.505 ± 0.948
1.081AlaHis: 1.081 ± 0.303
5.225AlaIle: 5.225 ± 0.74
5.225AlaLys: 5.225 ± 0.687
4.414AlaLeu: 4.414 ± 0.813
1.982AlaMet: 1.982 ± 0.563
2.072AlaAsn: 2.072 ± 0.43
1.712AlaPro: 1.712 ± 0.463
1.712AlaGln: 1.712 ± 0.535
2.703AlaArg: 2.703 ± 0.494
3.604AlaSer: 3.604 ± 0.801
4.775AlaThr: 4.775 ± 0.881
3.874AlaVal: 3.874 ± 0.976
1.081AlaTrp: 1.081 ± 0.355
1.351AlaTyr: 1.351 ± 0.518
0.0AlaXaa: 0.0 ± 0.0
Cys
0.18CysAla: 0.18 ± 0.13
0.0CysCys: 0.0 ± 0.0
0.901CysAsp: 0.901 ± 0.271
0.811CysGlu: 0.811 ± 0.303
0.18CysPhe: 0.18 ± 0.125
0.721CysGly: 0.721 ± 0.303
0.09CysHis: 0.09 ± 0.093
0.721CysIle: 0.721 ± 0.259
0.901CysLys: 0.901 ± 0.303
0.631CysLeu: 0.631 ± 0.282
0.36CysMet: 0.36 ± 0.224
0.45CysAsn: 0.45 ± 0.191
0.631CysPro: 0.631 ± 0.287
0.09CysGln: 0.09 ± 0.081
0.45CysArg: 0.45 ± 0.211
0.18CysSer: 0.18 ± 0.137
0.36CysThr: 0.36 ± 0.162
0.27CysVal: 0.27 ± 0.155
0.18CysTrp: 0.18 ± 0.123
0.27CysTyr: 0.27 ± 0.182
0.0CysXaa: 0.0 ± 0.0
Asp
4.414AspAla: 4.414 ± 0.733
0.45AspCys: 0.45 ± 0.262
1.982AspAsp: 1.982 ± 0.532
3.423AspGlu: 3.423 ± 0.522
2.342AspPhe: 2.342 ± 0.433
4.775AspGly: 4.775 ± 0.625
1.171AspHis: 1.171 ± 0.461
4.865AspIle: 4.865 ± 0.669
5.225AspLys: 5.225 ± 0.715
4.324AspLeu: 4.324 ± 0.601
1.892AspMet: 1.892 ± 0.348
2.432AspAsn: 2.432 ± 0.484
1.802AspPro: 1.802 ± 0.311
2.432AspGln: 2.432 ± 0.392
2.883AspArg: 2.883 ± 0.475
2.252AspSer: 2.252 ± 0.453
2.883AspThr: 2.883 ± 0.469
3.784AspVal: 3.784 ± 0.506
0.631AspTrp: 0.631 ± 0.184
2.432AspTyr: 2.432 ± 0.467
0.0AspXaa: 0.0 ± 0.0
Glu
6.396GluAla: 6.396 ± 0.956
0.901GluCys: 0.901 ± 0.319
2.883GluAsp: 2.883 ± 0.612
6.126GluGlu: 6.126 ± 1.111
3.514GluPhe: 3.514 ± 0.535
4.685GluGly: 4.685 ± 0.664
1.351GluHis: 1.351 ± 0.329
6.396GluIle: 6.396 ± 0.837
6.757GluLys: 6.757 ± 0.82
6.126GluLeu: 6.126 ± 0.532
4.324GluMet: 4.324 ± 0.563
4.595GluAsn: 4.595 ± 0.674
1.261GluPro: 1.261 ± 0.375
3.423GluGln: 3.423 ± 0.525
4.685GluArg: 4.685 ± 0.909
3.694GluSer: 3.694 ± 0.582
5.856GluThr: 5.856 ± 0.647
6.757GluVal: 6.757 ± 0.823
0.991GluTrp: 0.991 ± 0.289
2.973GluTyr: 2.973 ± 0.588
0.0GluXaa: 0.0 ± 0.0
Phe
2.072PheAla: 2.072 ± 0.328
0.18PheCys: 0.18 ± 0.126
2.432PheAsp: 2.432 ± 0.474
2.793PheGlu: 2.793 ± 0.497
0.991PhePhe: 0.991 ± 0.212
2.613PheGly: 2.613 ± 0.446
0.45PheHis: 0.45 ± 0.204
2.432PheIle: 2.432 ± 0.399
3.063PheLys: 3.063 ± 0.487
2.252PheLeu: 2.252 ± 0.436
1.532PheMet: 1.532 ± 0.245
2.342PheAsn: 2.342 ± 0.577
0.991PhePro: 0.991 ± 0.34
1.532PheGln: 1.532 ± 0.435
2.072PheArg: 2.072 ± 0.517
2.703PheSer: 2.703 ± 0.345
2.973PheThr: 2.973 ± 0.686
1.802PheVal: 1.802 ± 0.362
0.18PheTrp: 0.18 ± 0.114
1.712PheTyr: 1.712 ± 0.303
0.0PheXaa: 0.0 ± 0.0
Gly
2.342GlyAla: 2.342 ± 0.647
0.36GlyCys: 0.36 ± 0.251
2.883GlyAsp: 2.883 ± 0.389
5.495GlyGlu: 5.495 ± 0.83
3.243GlyPhe: 3.243 ± 0.731
3.784GlyGly: 3.784 ± 0.937
1.171GlyHis: 1.171 ± 0.275
4.595GlyIle: 4.595 ± 0.545
7.297GlyLys: 7.297 ± 0.677
4.324GlyLeu: 4.324 ± 0.949
2.342GlyMet: 2.342 ± 0.558
4.414GlyAsn: 4.414 ± 0.533
1.892GlyPro: 1.892 ± 0.469
2.252GlyGln: 2.252 ± 0.654
3.423GlyArg: 3.423 ± 0.677
4.054GlySer: 4.054 ± 0.627
4.775GlyThr: 4.775 ± 0.918
4.234GlyVal: 4.234 ± 0.769
1.441GlyTrp: 1.441 ± 0.355
3.784GlyTyr: 3.784 ± 0.675
0.0GlyXaa: 0.0 ± 0.0
His
1.081HisAla: 1.081 ± 0.399
0.0HisCys: 0.0 ± 0.0
1.532HisAsp: 1.532 ± 0.431
0.991HisGlu: 0.991 ± 0.31
0.901HisPhe: 0.901 ± 0.274
1.261HisGly: 1.261 ± 0.357
0.18HisHis: 0.18 ± 0.102
1.261HisIle: 1.261 ± 0.355
1.081HisLys: 1.081 ± 0.299
1.802HisLeu: 1.802 ± 0.463
0.36HisMet: 0.36 ± 0.167
1.171HisAsn: 1.171 ± 0.286
0.18HisPro: 0.18 ± 0.119
0.631HisGln: 0.631 ± 0.264
0.901HisArg: 0.901 ± 0.284
0.631HisSer: 0.631 ± 0.205
0.901HisThr: 0.901 ± 0.221
1.171HisVal: 1.171 ± 0.343
0.27HisTrp: 0.27 ± 0.123
1.081HisTyr: 1.081 ± 0.319
0.0HisXaa: 0.0 ± 0.0
Ile
4.144IleAla: 4.144 ± 0.512
0.541IleCys: 0.541 ± 0.191
5.225IleAsp: 5.225 ± 0.72
6.486IleGlu: 6.486 ± 0.786
1.351IlePhe: 1.351 ± 0.375
3.604IleGly: 3.604 ± 1.04
1.351IleHis: 1.351 ± 0.34
2.973IleIle: 2.973 ± 0.557
6.757IleLys: 6.757 ± 0.88
3.964IleLeu: 3.964 ± 0.524
1.712IleMet: 1.712 ± 0.343
4.685IleAsn: 4.685 ± 0.869
1.982IlePro: 1.982 ± 0.585
1.802IleGln: 1.802 ± 0.413
3.604IleArg: 3.604 ± 0.486
6.216IleSer: 6.216 ± 0.834
4.324IleThr: 4.324 ± 0.792
5.495IleVal: 5.495 ± 0.658
0.36IleTrp: 0.36 ± 0.183
2.703IleTyr: 2.703 ± 0.601
0.0IleXaa: 0.0 ± 0.0
Lys
4.775LysAla: 4.775 ± 0.716
0.541LysCys: 0.541 ± 0.199
4.234LysAsp: 4.234 ± 0.755
8.649LysGlu: 8.649 ± 0.914
2.162LysPhe: 2.162 ± 0.487
6.667LysGly: 6.667 ± 0.751
2.162LysHis: 2.162 ± 0.46
4.775LysIle: 4.775 ± 0.525
8.649LysLys: 8.649 ± 1.18
5.676LysLeu: 5.676 ± 0.729
3.153LysMet: 3.153 ± 0.526
3.964LysAsn: 3.964 ± 0.573
3.063LysPro: 3.063 ± 0.575
4.054LysGln: 4.054 ± 0.572
5.766LysArg: 5.766 ± 0.819
4.775LysSer: 4.775 ± 0.591
5.495LysThr: 5.495 ± 0.985
6.126LysVal: 6.126 ± 0.772
1.081LysTrp: 1.081 ± 0.322
2.973LysTyr: 2.973 ± 0.646
0.0LysXaa: 0.0 ± 0.0
Leu
4.054LeuAla: 4.054 ± 0.779
0.721LeuCys: 0.721 ± 0.28
5.135LeuAsp: 5.135 ± 0.599
6.306LeuGlu: 6.306 ± 1.076
2.162LeuPhe: 2.162 ± 0.418
4.324LeuGly: 4.324 ± 0.95
1.441LeuHis: 1.441 ± 0.387
4.685LeuIle: 4.685 ± 0.585
6.126LeuLys: 6.126 ± 0.571
5.766LeuLeu: 5.766 ± 0.619
2.342LeuMet: 2.342 ± 0.499
4.234LeuAsn: 4.234 ± 0.467
2.252LeuPro: 2.252 ± 0.472
2.883LeuGln: 2.883 ± 0.457
3.153LeuArg: 3.153 ± 0.529
5.135LeuSer: 5.135 ± 0.71
3.153LeuThr: 3.153 ± 0.494
4.595LeuVal: 4.595 ± 0.537
0.991LeuTrp: 0.991 ± 0.278
2.252LeuTyr: 2.252 ± 0.379
0.0LeuXaa: 0.0 ± 0.0
Met
1.892MetAla: 1.892 ± 0.65
0.18MetCys: 0.18 ± 0.119
1.892MetAsp: 1.892 ± 0.328
2.793MetGlu: 2.793 ± 0.486
1.712MetPhe: 1.712 ± 0.477
1.712MetGly: 1.712 ± 0.381
0.18MetHis: 0.18 ± 0.12
1.802MetIle: 1.802 ± 0.482
4.234MetLys: 4.234 ± 0.676
1.532MetLeu: 1.532 ± 0.355
0.811MetMet: 0.811 ± 0.396
2.883MetAsn: 2.883 ± 0.487
0.721MetPro: 0.721 ± 0.26
1.081MetGln: 1.081 ± 0.32
2.793MetArg: 2.793 ± 0.473
2.162MetSer: 2.162 ± 0.627
2.883MetThr: 2.883 ± 0.492
1.802MetVal: 1.802 ± 0.402
0.27MetTrp: 0.27 ± 0.144
1.081MetTyr: 1.081 ± 0.319
0.0MetXaa: 0.0 ± 0.0
Asn
3.423AsnAla: 3.423 ± 0.551
0.18AsnCys: 0.18 ± 0.136
4.865AsnAsp: 4.865 ± 0.815
5.586AsnGlu: 5.586 ± 0.553
1.441AsnPhe: 1.441 ± 0.345
4.955AsnGly: 4.955 ± 0.67
0.991AsnHis: 0.991 ± 0.266
2.973AsnIle: 2.973 ± 0.647
5.856AsnLys: 5.856 ± 0.837
3.423AsnLeu: 3.423 ± 0.526
2.252AsnMet: 2.252 ± 0.497
3.784AsnAsn: 3.784 ± 0.667
2.252AsnPro: 2.252 ± 0.418
2.252AsnGln: 2.252 ± 0.39
2.523AsnArg: 2.523 ± 0.547
2.793AsnSer: 2.793 ± 0.764
3.784AsnThr: 3.784 ± 0.437
2.793AsnVal: 2.793 ± 0.591
0.36AsnTrp: 0.36 ± 0.149
1.532AsnTyr: 1.532 ± 0.297
0.0AsnXaa: 0.0 ± 0.0
Pro
1.712ProAla: 1.712 ± 0.391
0.18ProCys: 0.18 ± 0.104
1.351ProAsp: 1.351 ± 0.281
2.432ProGlu: 2.432 ± 0.535
1.261ProPhe: 1.261 ± 0.382
2.072ProGly: 2.072 ± 0.492
0.36ProHis: 0.36 ± 0.171
1.712ProIle: 1.712 ± 0.433
2.613ProLys: 2.613 ± 0.487
2.342ProLeu: 2.342 ± 0.413
0.901ProMet: 0.901 ± 0.361
1.982ProAsn: 1.982 ± 0.422
1.081ProPro: 1.081 ± 0.263
0.811ProGln: 0.811 ± 0.32
1.261ProArg: 1.261 ± 0.507
1.622ProSer: 1.622 ± 0.414
1.351ProThr: 1.351 ± 0.291
1.892ProVal: 1.892 ± 0.387
0.36ProTrp: 0.36 ± 0.173
1.532ProTyr: 1.532 ± 0.377
0.0ProXaa: 0.0 ± 0.0
Gln
2.523GlnAla: 2.523 ± 0.526
0.45GlnCys: 0.45 ± 0.206
2.252GlnAsp: 2.252 ± 0.423
3.514GlnGlu: 3.514 ± 0.576
0.901GlnPhe: 0.901 ± 0.253
2.523GlnGly: 2.523 ± 0.492
0.541GlnHis: 0.541 ± 0.234
2.703GlnIle: 2.703 ± 0.372
2.523GlnLys: 2.523 ± 0.427
3.514GlnLeu: 3.514 ± 0.602
0.991GlnMet: 0.991 ± 0.342
1.982GlnAsn: 1.982 ± 0.407
1.261GlnPro: 1.261 ± 0.389
2.072GlnGln: 2.072 ± 0.587
1.712GlnArg: 1.712 ± 0.423
2.072GlnSer: 2.072 ± 0.539
1.622GlnThr: 1.622 ± 0.313
2.342GlnVal: 2.342 ± 0.327
0.36GlnTrp: 0.36 ± 0.132
1.712GlnTyr: 1.712 ± 0.304
0.0GlnXaa: 0.0 ± 0.0
Arg
2.523ArgAla: 2.523 ± 0.556
1.081ArgCys: 1.081 ± 0.324
2.162ArgAsp: 2.162 ± 0.364
3.784ArgGlu: 3.784 ± 0.604
1.892ArgPhe: 1.892 ± 0.479
2.973ArgGly: 2.973 ± 0.462
1.081ArgHis: 1.081 ± 0.309
4.144ArgIle: 4.144 ± 0.72
4.234ArgLys: 4.234 ± 0.753
4.234ArgLeu: 4.234 ± 0.63
2.162ArgMet: 2.162 ± 0.399
3.423ArgAsn: 3.423 ± 0.527
1.081ArgPro: 1.081 ± 0.342
2.162ArgGln: 2.162 ± 0.409
2.883ArgArg: 2.883 ± 0.578
2.162ArgSer: 2.162 ± 0.386
2.973ArgThr: 2.973 ± 0.611
3.604ArgVal: 3.604 ± 0.579
0.631ArgTrp: 0.631 ± 0.188
2.432ArgTyr: 2.432 ± 0.392
0.0ArgXaa: 0.0 ± 0.0
Ser
3.333SerAla: 3.333 ± 0.752
0.631SerCys: 0.631 ± 0.254
2.523SerAsp: 2.523 ± 0.543
4.324SerGlu: 4.324 ± 0.786
2.973SerPhe: 2.973 ± 0.507
4.144SerGly: 4.144 ± 0.641
0.631SerHis: 0.631 ± 0.316
3.874SerIle: 3.874 ± 0.678
4.234SerLys: 4.234 ± 0.576
4.775SerLeu: 4.775 ± 0.732
1.982SerMet: 1.982 ± 0.502
3.784SerAsn: 3.784 ± 0.76
1.081SerPro: 1.081 ± 0.276
1.982SerGln: 1.982 ± 0.33
1.982SerArg: 1.982 ± 0.455
2.162SerSer: 2.162 ± 0.467
2.973SerThr: 2.973 ± 0.559
3.423SerVal: 3.423 ± 0.625
1.081SerTrp: 1.081 ± 0.302
2.703SerTyr: 2.703 ± 0.575
0.0SerXaa: 0.0 ± 0.0
Thr
4.505ThrAla: 4.505 ± 0.604
0.45ThrCys: 0.45 ± 0.241
3.784ThrAsp: 3.784 ± 0.513
4.865ThrGlu: 4.865 ± 0.674
2.883ThrPhe: 2.883 ± 0.493
5.315ThrGly: 5.315 ± 0.584
0.811ThrHis: 0.811 ± 0.253
5.856ThrIle: 5.856 ± 0.572
5.495ThrLys: 5.495 ± 0.705
4.234ThrLeu: 4.234 ± 0.601
1.802ThrMet: 1.802 ± 0.444
3.243ThrAsn: 3.243 ± 0.697
2.432ThrPro: 2.432 ± 0.458
2.252ThrGln: 2.252 ± 0.58
3.153ThrArg: 3.153 ± 0.67
2.613ThrSer: 2.613 ± 0.575
2.883ThrThr: 2.883 ± 0.658
3.514ThrVal: 3.514 ± 0.522
0.27ThrTrp: 0.27 ± 0.134
2.342ThrTyr: 2.342 ± 0.448
0.0ThrXaa: 0.0 ± 0.0
Val
4.234ValAla: 4.234 ± 0.665
0.36ValCys: 0.36 ± 0.174
3.514ValAsp: 3.514 ± 0.532
5.946ValGlu: 5.946 ± 0.62
2.342ValPhe: 2.342 ± 0.52
3.694ValGly: 3.694 ± 0.509
1.351ValHis: 1.351 ± 0.302
5.225ValIle: 5.225 ± 0.753
5.045ValLys: 5.045 ± 0.494
4.144ValLeu: 4.144 ± 0.455
1.802ValMet: 1.802 ± 0.425
3.874ValAsn: 3.874 ± 0.673
1.802ValPro: 1.802 ± 0.392
2.523ValGln: 2.523 ± 0.401
2.613ValArg: 2.613 ± 0.502
2.883ValSer: 2.883 ± 0.375
5.766ValThr: 5.766 ± 0.817
3.694ValVal: 3.694 ± 0.714
1.261ValTrp: 1.261 ± 0.281
1.622ValTyr: 1.622 ± 0.387
0.0ValXaa: 0.0 ± 0.0
Trp
0.811TrpAla: 0.811 ± 0.399
0.27TrpCys: 0.27 ± 0.141
1.171TrpAsp: 1.171 ± 0.36
0.811TrpGlu: 0.811 ± 0.257
0.541TrpPhe: 0.541 ± 0.284
0.991TrpGly: 0.991 ± 0.311
0.18TrpHis: 0.18 ± 0.145
0.721TrpIle: 0.721 ± 0.24
0.721TrpLys: 0.721 ± 0.233
1.622TrpLeu: 1.622 ± 0.331
0.27TrpMet: 0.27 ± 0.123
0.541TrpAsn: 0.541 ± 0.275
0.18TrpPro: 0.18 ± 0.129
0.721TrpGln: 0.721 ± 0.219
0.36TrpArg: 0.36 ± 0.189
0.991TrpSer: 0.991 ± 0.258
0.631TrpThr: 0.631 ± 0.286
0.541TrpVal: 0.541 ± 0.195
0.18TrpTrp: 0.18 ± 0.134
0.541TrpTyr: 0.541 ± 0.24
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.982TyrAla: 1.982 ± 0.373
0.45TyrCys: 0.45 ± 0.212
2.973TyrAsp: 2.973 ± 0.454
3.333TyrGlu: 3.333 ± 0.611
1.622TyrPhe: 1.622 ± 0.445
2.793TyrGly: 2.793 ± 0.491
0.811TyrHis: 0.811 ± 0.269
2.342TyrIle: 2.342 ± 0.488
2.793TyrLys: 2.793 ± 0.562
2.613TyrLeu: 2.613 ± 0.491
1.351TyrMet: 1.351 ± 0.37
2.162TyrAsn: 2.162 ± 0.451
1.171TyrPro: 1.171 ± 0.315
0.901TyrGln: 0.901 ± 0.3
2.703TyrArg: 2.703 ± 0.446
1.892TyrSer: 1.892 ± 0.321
2.342TyrThr: 2.342 ± 0.406
1.982TyrVal: 1.982 ± 0.35
0.811TyrTrp: 0.811 ± 0.231
1.532TyrTyr: 1.532 ± 0.408
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (11101 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski