Amino acid dipepetide frequency for Trypanosoma brucei brucei (strain 927/4 GUTat10.1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.438AlaAla: 9.438 ± 0.092	1.534AlaCys: 1.534 ± 0.024	3.793AlaAsp: 3.793 ± 0.046	5.764AlaGlu: 5.764 ± 0.084	2.838AlaPhe: 2.838 ± 0.038	4.805AlaGly: 4.805 ± 0.055	1.768AlaHis: 1.768 ± 0.023	3.127AlaIle: 3.127 ± 0.032	3.516AlaLys: 3.516 ± 0.035	8.346AlaLeu: 8.346 ± 0.069	2.016AlaMet: 2.016 ± 0.037	2.582AlaAsn: 2.582 ± 0.029	3.858AlaPro: 3.858 ± 0.079	2.847AlaGln: 2.847 ± 0.036	4.844AlaArg: 4.844 ± 0.056	6.47AlaSer: 6.47 ± 0.055	5.068AlaThr: 5.068 ± 0.043	6.897AlaVal: 6.897 ± 0.043	0.736AlaTrp: 0.736 ± 0.013	1.77AlaTyr: 1.77 ± 0.031	0.0AlaXaa: 0.0 ± 0.0
Cys	1.799CysAla: 1.799 ± 0.041	0.726CysCys: 0.726 ± 0.019	1.186CysAsp: 1.186 ± 0.02	1.348CysGlu: 1.348 ± 0.019	0.978CysPhe: 0.978 ± 0.016	2.038CysGly: 2.038 ± 0.029	0.513CysHis: 0.513 ± 0.012	0.989CysIle: 0.989 ± 0.015	0.856CysLys: 0.856 ± 0.017	1.866CysLeu: 1.866 ± 0.026	0.467CysMet: 0.467 ± 0.01	0.843CysAsn: 0.843 ± 0.033	0.923CysPro: 0.923 ± 0.021	0.574CysGln: 0.574 ± 0.012	1.479CysArg: 1.479 ± 0.02	1.962CysSer: 1.962 ± 0.025	1.257CysThr: 1.257 ± 0.019	1.931CysVal: 1.931 ± 0.029	0.229CysTrp: 0.229 ± 0.007	0.55CysTyr: 0.55 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	4.627AspAla: 4.627 ± 0.042	1.016AspCys: 1.016 ± 0.043	3.719AspAsp: 3.719 ± 0.053	4.225AspGlu: 4.225 ± 0.077	1.768AspPhe: 1.768 ± 0.039	4.106AspGly: 4.106 ± 0.035	0.93AspHis: 0.93 ± 0.015	2.588AspIle: 2.588 ± 0.039	1.995AspLys: 1.995 ± 0.033	3.723AspLeu: 3.723 ± 0.04	1.24AspMet: 1.24 ± 0.022	1.779AspAsn: 1.779 ± 0.022	2.36AspPro: 2.36 ± 0.046	1.156AspGln: 1.156 ± 0.018	2.675AspArg: 2.675 ± 0.028	3.541AspSer: 3.541 ± 0.044	2.765AspThr: 2.765 ± 0.03	4.673AspVal: 4.673 ± 0.052	0.508AspTrp: 0.508 ± 0.02	1.267AspTyr: 1.267 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	6.091GluAla: 6.091 ± 0.085	1.337GluCys: 1.337 ± 0.02	3.706GluAsp: 3.706 ± 0.057	7.499GluGlu: 7.499 ± 0.124	1.866GluPhe: 1.866 ± 0.029	4.921GluGly: 4.921 ± 0.053	1.451GluHis: 1.451 ± 0.02	2.332GluIle: 2.332 ± 0.031	4.036GluLys: 4.036 ± 0.052	6.546GluLeu: 6.546 ± 0.101	1.743GluMet: 1.743 ± 0.029	2.463GluAsn: 2.463 ± 0.035	2.298GluPro: 2.298 ± 0.029	2.867GluGln: 2.867 ± 0.088	5.314GluArg: 5.314 ± 0.056	4.552GluSer: 4.552 ± 0.06	3.403GluThr: 3.403 ± 0.05	5.11GluVal: 5.11 ± 0.049	0.826GluTrp: 0.826 ± 0.015	1.577GluTyr: 1.577 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	2.774PheAla: 2.774 ± 0.03	0.935PheCys: 0.935 ± 0.017	1.919PheAsp: 1.919 ± 0.025	1.828PheGlu: 1.828 ± 0.028	2.083PhePhe: 2.083 ± 0.035	2.387PheGly: 2.387 ± 0.038	1.01PheHis: 1.01 ± 0.017	1.679PheIle: 1.679 ± 0.022	1.175PheLys: 1.175 ± 0.019	3.793PheLeu: 3.793 ± 0.039	0.874PheMet: 0.874 ± 0.012	1.281PheAsn: 1.281 ± 0.019	1.913PhePro: 1.913 ± 0.024	1.05PheGln: 1.05 ± 0.017	2.144PheArg: 2.144 ± 0.032	3.238PheSer: 3.238 ± 0.033	2.143PheThr: 2.143 ± 0.028	3.14PheVal: 3.14 ± 0.033	0.387PheTrp: 0.387 ± 0.01	1.016PheTyr: 1.016 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	5.258GlyAla: 5.258 ± 0.04	1.558GlyCys: 1.558 ± 0.023	3.997GlyAsp: 3.997 ± 0.044	4.662GlyGlu: 4.662 ± 0.035	2.164GlyPhe: 2.164 ± 0.032	6.089GlyGly: 6.089 ± 0.062	1.354GlyHis: 1.354 ± 0.018	2.652GlyIle: 2.652 ± 0.028	3.435GlyLys: 3.435 ± 0.035	4.664GlyLeu: 4.664 ± 0.039	1.487GlyMet: 1.487 ± 0.026	2.841GlyAsn: 2.841 ± 0.032	2.42GlyPro: 2.42 ± 0.051	1.84GlyGln: 1.84 ± 0.03	4.36GlyArg: 4.36 ± 0.04	5.753GlySer: 5.753 ± 0.059	3.986GlyThr: 3.986 ± 0.036	5.179GlyVal: 5.179 ± 0.043	0.697GlyTrp: 0.697 ± 0.014	1.593GlyTyr: 1.593 ± 0.022	0.0GlyXaa: 0.0 ± 0.0
His	1.745HisAla: 1.745 ± 0.023	0.64HisCys: 0.64 ± 0.014	1.138HisAsp: 1.138 ± 0.018	1.502HisGlu: 1.502 ± 0.035	1.033HisPhe: 1.033 ± 0.024	1.519HisGly: 1.519 ± 0.024	0.875HisHis: 0.875 ± 0.023	1.198HisIle: 1.198 ± 0.017	0.899HisLys: 0.899 ± 0.016	2.347HisLeu: 2.347 ± 0.027	0.612HisMet: 0.612 ± 0.013	0.958HisAsn: 0.958 ± 0.017	1.355HisPro: 1.355 ± 0.019	0.959HisGln: 0.959 ± 0.018	1.834HisArg: 1.834 ± 0.023	1.968HisSer: 1.968 ± 0.029	1.408HisThr: 1.408 ± 0.036	1.944HisVal: 1.944 ± 0.026	0.311HisTrp: 0.311 ± 0.009	0.748HisTyr: 0.748 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	3.371IleAla: 3.371 ± 0.038	0.938IleCys: 0.938 ± 0.014	2.141IleAsp: 2.141 ± 0.047	2.361IleGlu: 2.361 ± 0.027	1.577IlePhe: 1.577 ± 0.023	2.371IleGly: 2.371 ± 0.026	1.005IleHis: 1.005 ± 0.017	2.017IleIle: 2.017 ± 0.029	1.634IleLys: 1.634 ± 0.023	3.463IleLeu: 3.463 ± 0.031	0.953IleMet: 0.953 ± 0.037	1.523IleAsn: 1.523 ± 0.019	2.237IlePro: 2.237 ± 0.031	1.374IleGln: 1.374 ± 0.023	2.629IleArg: 2.629 ± 0.03	3.279IleSer: 3.279 ± 0.032	2.544IleThr: 2.544 ± 0.035	3.126IleVal: 3.126 ± 0.03	0.349IleTrp: 0.349 ± 0.009	1.09IleTyr: 1.09 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	3.51LysAla: 3.51 ± 0.042	0.928LysCys: 0.928 ± 0.017	2.326LysAsp: 2.326 ± 0.035	3.942LysGlu: 3.942 ± 0.061	1.245LysPhe: 1.245 ± 0.025	3.098LysGly: 3.098 ± 0.033	1.185LysHis: 1.185 ± 0.02	1.617LysIle: 1.617 ± 0.021	3.238LysLys: 3.238 ± 0.041	4.316LysLeu: 4.316 ± 0.048	1.091LysMet: 1.091 ± 0.017	1.706LysAsn: 1.706 ± 0.02	1.983LysPro: 1.983 ± 0.027	2.086LysGln: 2.086 ± 0.029	3.74LysArg: 3.74 ± 0.035	3.029LysSer: 3.029 ± 0.036	2.349LysThr: 2.349 ± 0.025	3.031LysVal: 3.031 ± 0.03	0.533LysTrp: 0.533 ± 0.012	1.211LysTyr: 1.211 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	6.619LeuAla: 6.619 ± 0.052	2.419LeuCys: 2.419 ± 0.028	4.187LeuAsp: 4.187 ± 0.051	5.859LeuGlu: 5.859 ± 0.065	3.957LeuPhe: 3.957 ± 0.039	4.806LeuGly: 4.806 ± 0.044	2.879LeuHis: 2.879 ± 0.031	3.325LeuIle: 3.325 ± 0.032	4.271LeuLys: 4.271 ± 0.045	11.049LeuLeu: 11.049 ± 0.084	2.165LeuMet: 2.165 ± 0.023	3.24LeuAsn: 3.24 ± 0.042	5.003LeuPro: 5.003 ± 0.038	4.85LeuGln: 4.85 ± 0.056	7.939LeuArg: 7.939 ± 0.075	7.799LeuSer: 7.799 ± 0.056	4.924LeuThr: 4.924 ± 0.035	6.119LeuVal: 6.119 ± 0.052	1.125LeuTrp: 1.125 ± 0.019	2.465LeuTyr: 2.465 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	1.635MetAla: 1.635 ± 0.022	0.532MetCys: 0.532 ± 0.011	1.187MetAsp: 1.187 ± 0.038	1.871MetGlu: 1.871 ± 0.039	0.815MetPhe: 0.815 ± 0.016	1.348MetGly: 1.348 ± 0.018	0.602MetHis: 0.602 ± 0.013	0.83MetIle: 0.83 ± 0.014	1.29MetLys: 1.29 ± 0.021	2.369MetLeu: 2.369 ± 0.029	0.684MetMet: 0.684 ± 0.017	0.908MetAsn: 0.908 ± 0.018	1.065MetPro: 1.065 ± 0.021	1.064MetGln: 1.064 ± 0.018	1.841MetArg: 1.841 ± 0.025	1.845MetSer: 1.845 ± 0.025	1.221MetThr: 1.221 ± 0.019	1.368MetVal: 1.368 ± 0.02	0.363MetTrp: 0.363 ± 0.011	0.672MetTyr: 0.672 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.253AsnAla: 3.253 ± 0.044	0.822AsnCys: 0.822 ± 0.019	2.04AsnAsp: 2.04 ± 0.03	2.637AsnGlu: 2.637 ± 0.059	1.248AsnPhe: 1.248 ± 0.019	2.744AsnGly: 2.744 ± 0.034	0.782AsnHis: 0.782 ± 0.014	1.864AsnIle: 1.864 ± 0.026	1.657AsnLys: 1.657 ± 0.021	2.675AsnLeu: 2.675 ± 0.03	0.897AsnMet: 0.897 ± 0.015	1.723AsnAsn: 1.723 ± 0.03	1.722AsnPro: 1.722 ± 0.02	1.013AsnGln: 1.013 ± 0.018	2.128AsnArg: 2.128 ± 0.024	2.921AsnSer: 2.921 ± 0.035	2.19AsnThr: 2.19 ± 0.028	2.878AsnVal: 2.878 ± 0.029	0.352AsnTrp: 0.352 ± 0.01	0.913AsnTyr: 0.913 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	3.469ProAla: 3.469 ± 0.044	0.98ProCys: 0.98 ± 0.039	1.972ProAsp: 1.972 ± 0.027	2.75ProGlu: 2.75 ± 0.049	2.047ProPhe: 2.047 ± 0.024	2.375ProGly: 2.375 ± 0.039	1.401ProHis: 1.401 ± 0.024	1.79ProIle: 1.79 ± 0.026	2.025ProLys: 2.025 ± 0.042	5.113ProLeu: 5.113 ± 0.047	0.99ProMet: 0.99 ± 0.023	1.68ProAsn: 1.68 ± 0.021	4.001ProPro: 4.001 ± 0.061	2.151ProGln: 2.151 ± 0.046	2.987ProArg: 2.987 ± 0.037	4.549ProSer: 4.549 ± 0.047	3.171ProThr: 3.171 ± 0.033	3.481ProVal: 3.481 ± 0.033	0.521ProTrp: 0.521 ± 0.013	1.222ProTyr: 1.222 ± 0.019	0.0ProXaa: 0.0 ± 0.0
Gln	2.368GlnAla: 2.368 ± 0.029	0.817GlnCys: 0.817 ± 0.016	1.277GlnAsp: 1.277 ± 0.019	2.566GlnGlu: 2.566 ± 0.045	1.109GlnPhe: 1.109 ± 0.019	1.935GlnGly: 1.935 ± 0.038	1.283GlnHis: 1.283 ± 0.038	1.234GlnIle: 1.234 ± 0.017	2.017GlnLys: 2.017 ± 0.027	4.497GlnLeu: 4.497 ± 0.05	0.953GlnMet: 0.953 ± 0.016	1.252GlnAsn: 1.252 ± 0.025	1.925GlnPro: 1.925 ± 0.051	3.245GlnGln: 3.245 ± 0.126	3.784GlnArg: 3.784 ± 0.045	2.516GlnSer: 2.516 ± 0.025	1.819GlnThr: 1.819 ± 0.04	2.211GlnVal: 2.211 ± 0.03	0.543GlnTrp: 0.543 ± 0.013	0.978GlnTyr: 0.978 ± 0.029	0.0GlnXaa: 0.0 ± 0.0
Arg	5.108ArgAla: 5.108 ± 0.051	1.777ArgCys: 1.777 ± 0.022	3.423ArgAsp: 3.423 ± 0.038	5.285ArgGlu: 5.285 ± 0.054	2.379ArgPhe: 2.379 ± 0.025	4.596ArgGly: 4.596 ± 0.042	1.983ArgHis: 1.983 ± 0.03	2.724ArgIle: 2.724 ± 0.031	3.44ArgLys: 3.44 ± 0.038	6.629ArgLeu: 6.629 ± 0.053	1.645ArgMet: 1.645 ± 0.021	2.558ArgAsn: 2.558 ± 0.03	2.638ArgPro: 2.638 ± 0.027	2.981ArgGln: 2.981 ± 0.033	6.508ArgArg: 6.508 ± 0.061	5.171ArgSer: 5.171 ± 0.055	3.369ArgThr: 3.369 ± 0.039	4.989ArgVal: 4.989 ± 0.042	0.938ArgTrp: 0.938 ± 0.018	1.967ArgTyr: 1.967 ± 0.023	0.0ArgXaa: 0.0 ± 0.0
Ser	6.56SerAla: 6.56 ± 0.056	1.807SerCys: 1.807 ± 0.026	3.897SerAsp: 3.897 ± 0.043	4.59SerGlu: 4.59 ± 0.059	3.192SerPhe: 3.192 ± 0.036	5.848SerGly: 5.848 ± 0.047	1.933SerHis: 1.933 ± 0.029	3.106SerIle: 3.106 ± 0.031	3.174SerLys: 3.174 ± 0.035	7.692SerLeu: 7.692 ± 0.062	1.718SerMet: 1.718 ± 0.028	2.955SerAsn: 2.955 ± 0.034	4.283SerPro: 4.283 ± 0.048	2.598SerGln: 2.598 ± 0.054	5.035SerArg: 5.035 ± 0.046	7.977SerSer: 7.977 ± 0.076	4.94SerThr: 4.94 ± 0.042	6.039SerVal: 6.039 ± 0.046	0.814SerTrp: 0.814 ± 0.014	1.857SerTyr: 1.857 ± 0.03	0.0SerXaa: 0.0 ± 0.0
Thr	5.292ThrAla: 5.292 ± 0.053	1.085ThrCys: 1.085 ± 0.017	2.675ThrAsp: 2.675 ± 0.033	3.517ThrGlu: 3.517 ± 0.048	2.111ThrPhe: 2.111 ± 0.028	3.65ThrGly: 3.65 ± 0.043	1.349ThrHis: 1.349 ± 0.018	2.332ThrIle: 2.332 ± 0.032	2.461ThrLys: 2.461 ± 0.027	5.418ThrLeu: 5.418 ± 0.046	1.227ThrMet: 1.227 ± 0.023	2.107ThrAsn: 2.107 ± 0.032	3.343ThrPro: 3.343 ± 0.037	1.921ThrGln: 1.921 ± 0.026	3.204ThrArg: 3.204 ± 0.029	4.818ThrSer: 4.818 ± 0.042	3.872ThrThr: 3.872 ± 0.05	4.475ThrVal: 4.475 ± 0.036	0.548ThrTrp: 0.548 ± 0.012	1.306ThrTyr: 1.306 ± 0.018	0.0ThrXaa: 0.0 ± 0.0
Val	6.496ValAla: 6.496 ± 0.051	1.71ValCys: 1.71 ± 0.025	4.072ValAsp: 4.072 ± 0.038	5.415ValGlu: 5.415 ± 0.045	2.801ValPhe: 2.801 ± 0.031	4.89ValGly: 4.89 ± 0.036	1.744ValHis: 1.744 ± 0.024	2.923ValIle: 2.923 ± 0.036	3.342ValLys: 3.342 ± 0.033	7.116ValLeu: 7.116 ± 0.052	1.807ValMet: 1.807 ± 0.024	2.511ValAsn: 2.511 ± 0.026	3.939ValPro: 3.939 ± 0.039	2.621ValGln: 2.621 ± 0.031	4.941ValArg: 4.941 ± 0.044	5.978ValSer: 5.978 ± 0.043	4.349ValThr: 4.349 ± 0.04	6.529ValVal: 6.529 ± 0.055	0.851ValTrp: 0.851 ± 0.02	1.815ValTyr: 1.815 ± 0.022	0.0ValXaa: 0.0 ± 0.0
Trp	0.708TrpAla: 0.708 ± 0.014	0.327TrpCys: 0.327 ± 0.009	0.599TrpAsp: 0.599 ± 0.019	0.762TrpGlu: 0.762 ± 0.015	0.397TrpPhe: 0.397 ± 0.011	0.69TrpGly: 0.69 ± 0.013	0.273TrpHis: 0.273 ± 0.009	0.414TrpIle: 0.414 ± 0.012	0.647TrpLys: 0.647 ± 0.013	1.059TrpLeu: 1.059 ± 0.019	0.341TrpMet: 0.341 ± 0.01	0.511TrpAsn: 0.511 ± 0.012	0.368TrpPro: 0.368 ± 0.01	0.362TrpGln: 0.362 ± 0.01	0.998TrpArg: 0.998 ± 0.019	0.866TrpSer: 0.866 ± 0.017	0.529TrpThr: 0.529 ± 0.013	0.718TrpVal: 0.718 ± 0.012	0.194TrpTrp: 0.194 ± 0.006	0.331TrpTyr: 0.331 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.898TyrAla: 1.898 ± 0.03	0.626TyrCys: 0.626 ± 0.013	1.406TyrAsp: 1.406 ± 0.018	1.558TyrGlu: 1.558 ± 0.029	1.162TyrPhe: 1.162 ± 0.016	1.773TyrGly: 1.773 ± 0.024	0.671TyrHis: 0.671 ± 0.011	1.243TyrIle: 1.243 ± 0.019	1.055TyrLys: 1.055 ± 0.026	2.321TyrLeu: 2.321 ± 0.027	0.598TyrMet: 0.598 ± 0.012	1.037TyrAsn: 1.037 ± 0.015	1.041TyrPro: 1.041 ± 0.017	0.772TyrGln: 0.772 ± 0.014	1.723TyrArg: 1.723 ± 0.023	1.784TyrSer: 1.784 ± 0.024	1.403TyrThr: 1.403 ± 0.02	2.009TyrVal: 2.009 ± 0.026	0.269TyrTrp: 0.269 ± 0.008	0.86TyrTyr: 0.86 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.004XaaXaa: 0.004 ± 0.004

Statistics based on 8587 proteins (4345578 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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