Sphingobium baderi LL03

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; Sphingobium baderi

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4697 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0HDB5|T0HDB5_9SPHN Formate dehydrogenase OS=Sphingobium baderi LL03 OX=1114964 GN=L485_21070 PE=3 SV=1
MM1 pKa = 7.12RR2 pKa = 11.84TTYY5 pKa = 10.7EE6 pKa = 4.03SATVRR11 pKa = 11.84LYY13 pKa = 10.82HH14 pKa = 7.17LSDD17 pKa = 3.54AQEE20 pKa = 4.34GGAATTLFYY29 pKa = 11.34GPLNEE34 pKa = 4.82ALLIAEE40 pKa = 4.76GQPADD45 pKa = 3.8VQDD48 pKa = 4.48SLFLATDD55 pKa = 3.65NDD57 pKa = 3.68VVAYY61 pKa = 10.42LDD63 pKa = 4.8LIGGG67 pKa = 4.02

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0GCP9|T0GCP9_9SPHN Amidohydro-rel domain-containing protein OS=Sphingobium baderi LL03 OX=1114964 GN=L485_17365 PE=4 SV=1
MM1 pKa = 7.33TLGSAMTGGIVRR13 pKa = 11.84VGRR16 pKa = 11.84MIAAARR22 pKa = 11.84RR23 pKa = 11.84HH24 pKa = 5.89RR25 pKa = 11.84LRR27 pKa = 11.84LMNRR31 pKa = 3.32

Molecular weight: 3.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4697

0

4697

1382595

29

2899

294.4

31.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.052 ± 0.052

0.829 ± 0.011

5.977 ± 0.026

5.502 ± 0.032

3.481 ± 0.022

8.817 ± 0.046

2.096 ± 0.021

5.222 ± 0.025

3.055 ± 0.027

9.987 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.636 ± 0.019

2.498 ± 0.023

5.174 ± 0.025

3.223 ± 0.018

7.607 ± 0.043

5.318 ± 0.027

5.009 ± 0.031

6.942 ± 0.029

1.397 ± 0.014

2.179 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski