Sphingobacterium sp. Ag1

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium; unclassified Sphingobacterium

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M2XUP0|A0A0M2XUP0_9SPHI DNA-binding protein OS=Sphingobacterium sp. Ag1 OX=1643451 GN=AAW12_20895 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.19KK3 pKa = 9.64IEE5 pKa = 4.31LVPLVGIQIEE15 pKa = 4.97GIGQVNLGAGLAEE28 pKa = 4.15VQGVLGAPSDD38 pKa = 4.03ALDD41 pKa = 3.61DD42 pKa = 3.54QYY44 pKa = 11.9YY45 pKa = 10.19YY46 pKa = 11.17DD47 pKa = 4.18EE48 pKa = 5.93LEE50 pKa = 4.36LRR52 pKa = 11.84LDD54 pKa = 4.1FNDD57 pKa = 3.25QGTLEE62 pKa = 4.87FIEE65 pKa = 5.27SINGPYY71 pKa = 9.61PEE73 pKa = 4.01KK74 pKa = 10.63TEE76 pKa = 3.83INIYY80 pKa = 9.74GVNPFQVEE88 pKa = 3.7AAEE91 pKa = 4.25LVNLLTDD98 pKa = 3.91KK99 pKa = 11.32NQGEE103 pKa = 4.25VDD105 pKa = 3.7DD106 pKa = 5.95AEE108 pKa = 5.54AGYY111 pKa = 10.68CYY113 pKa = 10.21TFLNSSVGVWRR124 pKa = 11.84QITEE128 pKa = 3.68EE129 pKa = 4.03DD130 pKa = 3.54VQEE133 pKa = 4.37EE134 pKa = 4.21IEE136 pKa = 4.38EE137 pKa = 4.05IKK139 pKa = 10.94ADD141 pKa = 4.78GEE143 pKa = 4.52YY144 pKa = 10.82EE145 pKa = 4.26DD146 pKa = 4.31NADD149 pKa = 4.5MLLEE153 pKa = 4.44DD154 pKa = 4.34LKK156 pKa = 11.0KK157 pKa = 11.04SKK159 pKa = 10.51FFWTIGIGVAGYY171 pKa = 9.55YY172 pKa = 10.58AEE174 pKa = 4.18

Molecular weight: 19.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M2XSE3|A0A0M2XSE3_9SPHI Uncharacterized protein OS=Sphingobacterium sp. Ag1 OX=1643451 GN=AAW12_16030 PE=4 SV=1
MM1 pKa = 7.21SQLKK5 pKa = 10.26KK6 pKa = 10.85LFIRR10 pKa = 11.84GVLEE14 pKa = 4.01EE15 pKa = 3.85EE16 pKa = 4.3GRR18 pKa = 11.84RR19 pKa = 11.84YY20 pKa = 10.39LSNQGRR26 pKa = 11.84EE27 pKa = 4.0IRR29 pKa = 11.84AKK31 pKa = 10.5LHH33 pKa = 4.64FHH35 pKa = 5.69TRR37 pKa = 11.84RR38 pKa = 11.84LFNDD42 pKa = 2.72RR43 pKa = 11.84TMNVVSASDD52 pKa = 3.32RR53 pKa = 11.84YY54 pKa = 9.47EE55 pKa = 3.9GKK57 pKa = 10.83LIITFPNYY65 pKa = 10.07LRR67 pKa = 11.84LLDD70 pKa = 3.53ARR72 pKa = 11.84RR73 pKa = 11.84NVKK76 pKa = 10.25DD77 pKa = 3.33RR78 pKa = 11.84TGKK81 pKa = 10.27RR82 pKa = 11.84SRR84 pKa = 11.84KK85 pKa = 9.62GYY87 pKa = 8.89QLYY90 pKa = 10.96NKK92 pKa = 9.42FAMGHH97 pKa = 5.67YY98 pKa = 9.43YY99 pKa = 10.67AIAHH103 pKa = 7.02RR104 pKa = 11.84LQNDD108 pKa = 3.5FTDD111 pKa = 5.08DD112 pKa = 3.03VALNLRR118 pKa = 11.84RR119 pKa = 11.84QWQQSNPP126 pKa = 3.5

Molecular weight: 15.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4687

0

4687

1679683

36

2183

358.4

40.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.181 ± 0.034

0.716 ± 0.01

5.522 ± 0.022

5.805 ± 0.036

4.977 ± 0.026

6.771 ± 0.038

1.857 ± 0.018

7.216 ± 0.037

7.029 ± 0.026

9.612 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.243 ± 0.016

5.675 ± 0.038

3.615 ± 0.017

3.999 ± 0.019

4.064 ± 0.018

6.474 ± 0.03

5.486 ± 0.027

6.196 ± 0.028

1.239 ± 0.014

4.324 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski