Amino acid dipepetide frequency for Sphingobacterium sp. Ag1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.719AlaAla: 5.719 ± 0.069
0.586AlaCys: 0.586 ± 0.02
4.043AlaAsp: 4.043 ± 0.054
4.251AlaGlu: 4.251 ± 0.053
3.33AlaPhe: 3.33 ± 0.053
5.013AlaGly: 5.013 ± 0.059
1.204AlaHis: 1.204 ± 0.028
5.515AlaIle: 5.515 ± 0.07
4.849AlaLys: 4.849 ± 0.056
7.076AlaLeu: 7.076 ± 0.088
1.653AlaMet: 1.653 ± 0.035
3.773AlaAsn: 3.773 ± 0.054
2.159AlaPro: 2.159 ± 0.043
3.087AlaGln: 3.087 ± 0.045
2.43AlaArg: 2.43 ± 0.035
4.676AlaSer: 4.676 ± 0.054
3.91AlaThr: 3.91 ± 0.055
4.729AlaVal: 4.729 ± 0.057
0.798AlaTrp: 0.798 ± 0.022
3.01AlaTyr: 3.01 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.473CysAla: 0.473 ± 0.017
0.123CysCys: 0.123 ± 0.01
0.335CysAsp: 0.335 ± 0.014
0.343CysGlu: 0.343 ± 0.015
0.389CysPhe: 0.389 ± 0.017
0.561CysGly: 0.561 ± 0.02
0.193CysHis: 0.193 ± 0.01
0.565CysIle: 0.565 ± 0.021
0.401CysLys: 0.401 ± 0.015
0.711CysLeu: 0.711 ± 0.02
0.174CysMet: 0.174 ± 0.011
0.324CysAsn: 0.324 ± 0.015
0.281CysPro: 0.281 ± 0.016
0.282CysGln: 0.282 ± 0.014
0.274CysArg: 0.274 ± 0.014
0.521CysSer: 0.521 ± 0.019
0.414CysThr: 0.414 ± 0.017
0.395CysVal: 0.395 ± 0.015
0.07CysTrp: 0.07 ± 0.006
0.328CysTyr: 0.328 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
3.838AspAla: 3.838 ± 0.05
0.345AspCys: 0.345 ± 0.016
2.426AspAsp: 2.426 ± 0.043
3.311AspGlu: 3.311 ± 0.055
3.298AspPhe: 3.298 ± 0.046
3.906AspGly: 3.906 ± 0.063
1.156AspHis: 1.156 ± 0.028
4.092AspIle: 4.092 ± 0.05
3.885AspLys: 3.885 ± 0.053
5.432AspLeu: 5.432 ± 0.06
1.192AspMet: 1.192 ± 0.029
2.943AspAsn: 2.943 ± 0.039
2.245AspPro: 2.245 ± 0.035
2.305AspGln: 2.305 ± 0.039
2.577AspArg: 2.577 ± 0.048
3.018AspSer: 3.018 ± 0.05
2.292AspThr: 2.292 ± 0.036
3.343AspVal: 3.343 ± 0.051
0.826AspTrp: 0.826 ± 0.023
2.786AspTyr: 2.786 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
4.108GluAla: 4.108 ± 0.059
0.303GluCys: 0.303 ± 0.014
2.898GluAsp: 2.898 ± 0.048
3.945GluGlu: 3.945 ± 0.056
2.49GluPhe: 2.49 ± 0.042
3.491GluGly: 3.491 ± 0.051
1.183GluHis: 1.183 ± 0.025
4.576GluIle: 4.576 ± 0.059
4.884GluLys: 4.884 ± 0.06
5.857GluLeu: 5.857 ± 0.068
1.398GluMet: 1.398 ± 0.03
3.581GluAsn: 3.581 ± 0.047
1.537GluPro: 1.537 ± 0.031
2.606GluGln: 2.606 ± 0.046
2.71GluArg: 2.71 ± 0.042
3.207GluSer: 3.207 ± 0.041
2.908GluThr: 2.908 ± 0.041
3.694GluVal: 3.694 ± 0.053
0.671GluTrp: 0.671 ± 0.02
2.005GluTyr: 2.005 ± 0.038
0.0GluXaa: 0.0 ± 0.0
Phe
3.287PheAla: 3.287 ± 0.05
0.41PheCys: 0.41 ± 0.017
3.242PheAsp: 3.242 ± 0.05
2.937PheGlu: 2.937 ± 0.042
2.568PhePhe: 2.568 ± 0.052
3.567PheGly: 3.567 ± 0.049
0.897PheHis: 0.897 ± 0.023
3.389PheIle: 3.389 ± 0.055
3.241PheLys: 3.241 ± 0.049
4.358PheLeu: 4.358 ± 0.058
1.196PheMet: 1.196 ± 0.029
2.884PheAsn: 2.884 ± 0.049
1.762PhePro: 1.762 ± 0.035
1.758PheGln: 1.758 ± 0.03
1.924PheArg: 1.924 ± 0.034
3.745PheSer: 3.745 ± 0.05
2.773PheThr: 2.773 ± 0.043
3.007PheVal: 3.007 ± 0.046
0.629PheTrp: 0.629 ± 0.021
2.19PheTyr: 2.19 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
4.568GlyAla: 4.568 ± 0.059
0.523GlyCys: 0.523 ± 0.019
3.377GlyAsp: 3.377 ± 0.048
3.416GlyGlu: 3.416 ± 0.051
3.524GlyPhe: 3.524 ± 0.052
4.691GlyGly: 4.691 ± 0.066
1.203GlyHis: 1.203 ± 0.029
5.29GlyIle: 5.29 ± 0.053
5.356GlyLys: 5.356 ± 0.056
6.36GlyLeu: 6.36 ± 0.068
1.703GlyMet: 1.703 ± 0.034
3.823GlyAsn: 3.823 ± 0.06
1.513GlyPro: 1.513 ± 0.033
2.645GlyGln: 2.645 ± 0.044
2.714GlyArg: 2.714 ± 0.046
4.304GlySer: 4.304 ± 0.062
4.12GlyThr: 4.12 ± 0.061
4.367GlyVal: 4.367 ± 0.05
0.96GlyTrp: 0.96 ± 0.026
3.258GlyTyr: 3.258 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
1.217HisAla: 1.217 ± 0.028
0.183HisCys: 0.183 ± 0.01
0.904HisAsp: 0.904 ± 0.023
1.018HisGlu: 1.018 ± 0.025
1.125HisPhe: 1.125 ± 0.025
1.162HisGly: 1.162 ± 0.028
0.513HisHis: 0.513 ± 0.021
1.476HisIle: 1.476 ± 0.031
1.004HisLys: 1.004 ± 0.027
1.845HisLeu: 1.845 ± 0.029
0.391HisMet: 0.391 ± 0.014
0.926HisAsn: 0.926 ± 0.028
0.903HisPro: 0.903 ± 0.025
0.784HisGln: 0.784 ± 0.023
0.808HisArg: 0.808 ± 0.021
1.051HisSer: 1.051 ± 0.024
0.945HisThr: 0.945 ± 0.025
1.086HisVal: 1.086 ± 0.027
0.292HisTrp: 0.292 ± 0.014
0.939HisTyr: 0.939 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
5.889IleAla: 5.889 ± 0.061
0.641IleCys: 0.641 ± 0.021
4.454IleAsp: 4.454 ± 0.05
4.243IleGlu: 4.243 ± 0.059
3.248IlePhe: 3.248 ± 0.056
5.176IleGly: 5.176 ± 0.068
1.393IleHis: 1.393 ± 0.027
4.65IleIle: 4.65 ± 0.066
4.673IleLys: 4.673 ± 0.057
6.492IleLeu: 6.492 ± 0.081
1.32IleMet: 1.32 ± 0.033
4.013IleAsn: 4.013 ± 0.056
3.17IlePro: 3.17 ± 0.047
2.864IleGln: 2.864 ± 0.049
3.073IleArg: 3.073 ± 0.044
4.959IleSer: 4.959 ± 0.055
3.879IleThr: 3.879 ± 0.052
4.525IleVal: 4.525 ± 0.054
0.783IleTrp: 0.783 ± 0.023
2.717IleTyr: 2.717 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
4.878LysAla: 4.878 ± 0.062
0.268LysCys: 0.268 ± 0.012
4.394LysAsp: 4.394 ± 0.059
4.981LysGlu: 4.981 ± 0.057
2.72LysPhe: 2.72 ± 0.044
4.541LysGly: 4.541 ± 0.054
1.19LysHis: 1.19 ± 0.029
5.059LysIle: 5.059 ± 0.06
5.437LysLys: 5.437 ± 0.072
6.266LysLeu: 6.266 ± 0.063
1.822LysMet: 1.822 ± 0.033
4.333LysAsn: 4.333 ± 0.051
2.387LysPro: 2.387 ± 0.035
2.775LysGln: 2.775 ± 0.046
2.917LysArg: 2.917 ± 0.043
4.335LysSer: 4.335 ± 0.047
4.022LysThr: 4.022 ± 0.052
4.301LysVal: 4.301 ± 0.06
0.81LysTrp: 0.81 ± 0.021
2.854LysTyr: 2.854 ± 0.043
0.0LysXaa: 0.0 ± 0.0
Leu
7.101LeuAla: 7.101 ± 0.076
0.783LeuCys: 0.783 ± 0.02
5.312LeuAsp: 5.312 ± 0.059
5.317LeuGlu: 5.317 ± 0.067
4.886LeuPhe: 4.886 ± 0.06
6.165LeuGly: 6.165 ± 0.058
1.742LeuHis: 1.742 ± 0.039
6.419LeuIle: 6.419 ± 0.084
7.086LeuLys: 7.086 ± 0.067
9.574LeuLeu: 9.574 ± 0.112
2.203LeuMet: 2.203 ± 0.039
5.443LeuAsn: 5.443 ± 0.063
4.023LeuPro: 4.023 ± 0.05
3.669LeuGln: 3.669 ± 0.051
3.906LeuArg: 3.906 ± 0.054
7.005LeuSer: 7.005 ± 0.074
5.328LeuThr: 5.328 ± 0.059
5.534LeuVal: 5.534 ± 0.068
0.995LeuTrp: 0.995 ± 0.029
3.624LeuTyr: 3.624 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
1.715MetAla: 1.715 ± 0.033
0.142MetCys: 0.142 ± 0.01
1.36MetAsp: 1.36 ± 0.033
1.426MetGlu: 1.426 ± 0.028
0.786MetPhe: 0.786 ± 0.022
1.565MetGly: 1.565 ± 0.029
0.392MetHis: 0.392 ± 0.017
1.482MetIle: 1.482 ± 0.03
1.93MetLys: 1.93 ± 0.034
2.106MetLeu: 2.106 ± 0.041
0.602MetMet: 0.602 ± 0.019
1.31MetAsn: 1.31 ± 0.027
0.947MetPro: 0.947 ± 0.023
0.898MetGln: 0.898 ± 0.027
0.963MetArg: 0.963 ± 0.023
1.36MetSer: 1.36 ± 0.031
1.206MetThr: 1.206 ± 0.028
1.376MetVal: 1.376 ± 0.028
0.193MetTrp: 0.193 ± 0.012
0.667MetTyr: 0.667 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.867AsnAla: 3.867 ± 0.05
0.344AsnCys: 0.344 ± 0.017
2.756AsnAsp: 2.756 ± 0.039
2.979AsnGlu: 2.979 ± 0.046
2.792AsnPhe: 2.792 ± 0.04
4.138AsnGly: 4.138 ± 0.063
0.967AsnHis: 0.967 ± 0.024
4.019AsnIle: 4.019 ± 0.058
3.844AsnLys: 3.844 ± 0.05
5.268AsnLeu: 5.268 ± 0.064
1.253AsnMet: 1.253 ± 0.026
3.402AsnAsn: 3.402 ± 0.062
2.638AsnPro: 2.638 ± 0.041
2.254AsnGln: 2.254 ± 0.037
2.527AsnArg: 2.527 ± 0.037
3.566AsnSer: 3.566 ± 0.052
3.243AsnThr: 3.243 ± 0.058
3.205AsnVal: 3.205 ± 0.047
0.813AsnTrp: 0.813 ± 0.021
2.88AsnTyr: 2.88 ± 0.051
0.0AsnXaa: 0.0 ± 0.0
Pro
2.605ProAla: 2.605 ± 0.039
0.202ProCys: 0.202 ± 0.011
2.187ProAsp: 2.187 ± 0.039
2.546ProGlu: 2.546 ± 0.041
1.939ProPhe: 1.939 ± 0.035
2.229ProGly: 2.229 ± 0.038
0.66ProHis: 0.66 ± 0.021
2.749ProIle: 2.749 ± 0.043
2.346ProLys: 2.346 ± 0.038
3.38ProLeu: 3.38 ± 0.046
0.788ProMet: 0.788 ± 0.025
2.137ProAsn: 2.137 ± 0.039
0.824ProPro: 0.824 ± 0.023
1.425ProGln: 1.425 ± 0.029
1.112ProArg: 1.112 ± 0.025
2.339ProSer: 2.339 ± 0.034
2.175ProThr: 2.175 ± 0.036
2.452ProVal: 2.452 ± 0.038
0.438ProTrp: 0.438 ± 0.013
1.616ProTyr: 1.616 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
2.732GlnAla: 2.732 ± 0.04
0.198GlnCys: 0.198 ± 0.01
2.02GlnAsp: 2.02 ± 0.03
2.48GlnGlu: 2.48 ± 0.041
1.88GlnPhe: 1.88 ± 0.035
2.358GlnGly: 2.358 ± 0.039
0.943GlnHis: 0.943 ± 0.027
2.755GlnIle: 2.755 ± 0.042
2.792GlnLys: 2.792 ± 0.039
4.481GlnLeu: 4.481 ± 0.062
0.878GlnMet: 0.878 ± 0.024
2.124GlnAsn: 2.124 ± 0.035
1.289GlnPro: 1.289 ± 0.028
2.279GlnGln: 2.279 ± 0.041
1.74GlnArg: 1.74 ± 0.032
2.262GlnSer: 2.262 ± 0.042
2.015GlnThr: 2.015 ± 0.038
2.521GlnVal: 2.521 ± 0.035
0.517GlnTrp: 0.517 ± 0.018
1.724GlnTyr: 1.724 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
2.596ArgAla: 2.596 ± 0.039
0.236ArgCys: 0.236 ± 0.012
2.125ArgAsp: 2.125 ± 0.035
2.406ArgGlu: 2.406 ± 0.04
2.195ArgPhe: 2.195 ± 0.039
2.277ArgGly: 2.277 ± 0.042
0.711ArgHis: 0.711 ± 0.019
3.329ArgIle: 3.329 ± 0.04
2.976ArgLys: 2.976 ± 0.045
3.965ArgLeu: 3.965 ± 0.052
1.038ArgMet: 1.038 ± 0.021
2.375ArgAsn: 2.375 ± 0.039
1.353ArgPro: 1.353 ± 0.031
1.572ArgGln: 1.572 ± 0.032
1.654ArgArg: 1.654 ± 0.035
2.505ArgSer: 2.505 ± 0.039
2.225ArgThr: 2.225 ± 0.038
2.421ArgVal: 2.421 ± 0.04
0.604ArgTrp: 0.604 ± 0.019
2.073ArgTyr: 2.073 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
4.475SerAla: 4.475 ± 0.05
0.633SerCys: 0.633 ± 0.022
3.286SerAsp: 3.286 ± 0.049
3.144SerGlu: 3.144 ± 0.047
3.735SerPhe: 3.735 ± 0.049
4.598SerGly: 4.598 ± 0.055
1.114SerHis: 1.114 ± 0.026
4.946SerIle: 4.946 ± 0.055
4.343SerLys: 4.343 ± 0.052
6.364SerLeu: 6.364 ± 0.058
1.309SerMet: 1.309 ± 0.032
3.549SerAsn: 3.549 ± 0.043
2.436SerPro: 2.436 ± 0.041
2.113SerGln: 2.113 ± 0.036
2.5SerArg: 2.5 ± 0.038
4.57SerSer: 4.57 ± 0.073
3.807SerThr: 3.807 ± 0.053
3.851SerVal: 3.851 ± 0.054
0.862SerTrp: 0.862 ± 0.025
3.109SerTyr: 3.109 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
4.46ThrAla: 4.46 ± 0.053
0.331ThrCys: 0.331 ± 0.015
3.114ThrAsp: 3.114 ± 0.044
2.959ThrGlu: 2.959 ± 0.042
2.78ThrPhe: 2.78 ± 0.042
4.241ThrGly: 4.241 ± 0.061
0.932ThrHis: 0.932 ± 0.023
4.116ThrIle: 4.116 ± 0.052
3.413ThrLys: 3.413 ± 0.05
5.364ThrLeu: 5.364 ± 0.054
1.021ThrMet: 1.021 ± 0.023
2.82ThrAsn: 2.82 ± 0.044
2.198ThrPro: 2.198 ± 0.039
1.866ThrGln: 1.866 ± 0.035
1.746ThrArg: 1.746 ± 0.033
3.395ThrSer: 3.395 ± 0.045
3.106ThrThr: 3.106 ± 0.052
3.901ThrVal: 3.901 ± 0.057
0.669ThrTrp: 0.669 ± 0.018
2.422ThrTyr: 2.422 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
4.467ValAla: 4.467 ± 0.057
0.462ValCys: 0.462 ± 0.015
3.829ValAsp: 3.829 ± 0.046
3.546ValGlu: 3.546 ± 0.046
3.043ValPhe: 3.043 ± 0.045
4.091ValGly: 4.091 ± 0.054
1.061ValHis: 1.061 ± 0.029
4.298ValIle: 4.298 ± 0.055
4.234ValLys: 4.234 ± 0.048
5.986ValLeu: 5.986 ± 0.066
1.288ValMet: 1.288 ± 0.03
3.593ValAsn: 3.593 ± 0.051
2.354ValPro: 2.354 ± 0.036
2.347ValGln: 2.347 ± 0.034
2.458ValArg: 2.458 ± 0.037
4.293ValSer: 4.293 ± 0.052
3.281ValThr: 3.281 ± 0.051
4.206ValVal: 4.206 ± 0.06
0.685ValTrp: 0.685 ± 0.02
2.442ValTyr: 2.442 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
0.779TrpAla: 0.779 ± 0.024
0.112TrpCys: 0.112 ± 0.008
0.692TrpAsp: 0.692 ± 0.021
0.697TrpGlu: 0.697 ± 0.019
0.549TrpPhe: 0.549 ± 0.016
0.941TrpGly: 0.941 ± 0.027
0.256TrpHis: 0.256 ± 0.012
0.837TrpIle: 0.837 ± 0.026
0.96TrpLys: 0.96 ± 0.027
1.129TrpLeu: 1.129 ± 0.025
0.367TrpMet: 0.367 ± 0.014
0.803TrpAsn: 0.803 ± 0.021
0.346TrpPro: 0.346 ± 0.015
0.513TrpGln: 0.513 ± 0.02
0.541TrpArg: 0.541 ± 0.019
0.786TrpSer: 0.786 ± 0.023
0.723TrpThr: 0.723 ± 0.02
0.647TrpVal: 0.647 ± 0.02
0.189TrpTrp: 0.189 ± 0.011
0.525TrpTyr: 0.525 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.036TyrAla: 3.036 ± 0.045
0.332TyrCys: 0.332 ± 0.014
2.463TyrAsp: 2.463 ± 0.046
2.106TyrGlu: 2.106 ± 0.034
2.489TyrPhe: 2.489 ± 0.042
3.002TyrGly: 3.002 ± 0.048
0.863TyrHis: 0.863 ± 0.023
2.596TyrIle: 2.596 ± 0.041
2.635TyrLys: 2.635 ± 0.041
4.104TyrLeu: 4.104 ± 0.052
0.828TyrMet: 0.828 ± 0.023
2.598TyrAsn: 2.598 ± 0.047
1.785TyrPro: 1.785 ± 0.036
1.945TyrGln: 1.945 ± 0.033
2.065TyrArg: 2.065 ± 0.039
2.845TyrSer: 2.845 ± 0.045
2.484TyrThr: 2.484 ± 0.04
2.405TyrVal: 2.405 ± 0.041
0.588TyrTrp: 0.588 ± 0.018
2.074TyrTyr: 2.074 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4687 proteins (1679683 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski