Anaerobacillus alkalilacustris

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Anaerobacillus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3502 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S2LJI9|A0A1S2LJI9_9BACI Uncharacterized protein OS=Anaerobacillus alkalilacustris OX=393763 GN=BKP37_12935 PE=4 SV=1
MM1 pKa = 7.63IDD3 pKa = 3.62KK4 pKa = 10.9NYY6 pKa = 10.01GAYY9 pKa = 10.51VLICDD14 pKa = 3.54ICGNEE19 pKa = 4.08ADD21 pKa = 4.14QSFDD25 pKa = 3.41TFDD28 pKa = 3.46NAIDD32 pKa = 3.94AKK34 pKa = 11.32NEE36 pKa = 4.05LGWRR40 pKa = 11.84SEE42 pKa = 4.01RR43 pKa = 11.84GEE45 pKa = 3.98QLDD48 pKa = 5.51LKK50 pKa = 10.85DD51 pKa = 4.03GWVDD55 pKa = 4.63LCPDD59 pKa = 3.75CQQ61 pKa = 3.53

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S2LXP0|A0A1S2LXP0_9BACI Amidohydrolase OS=Anaerobacillus alkalilacustris OX=393763 GN=BKP37_01890 PE=4 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.22KK15 pKa = 8.69VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTANGRR29 pKa = 11.84KK30 pKa = 8.48VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3502

0

3502

1032631

26

1767

294.9

33.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.207 ± 0.039

0.819 ± 0.014

4.987 ± 0.032

7.638 ± 0.046

4.675 ± 0.029

6.54 ± 0.035

2.046 ± 0.021

8.473 ± 0.045

7.274 ± 0.039

9.78 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.602 ± 0.017

4.894 ± 0.026

3.433 ± 0.025

3.64 ± 0.023

3.986 ± 0.032

5.954 ± 0.031

5.385 ± 0.029

7.131 ± 0.034

0.97 ± 0.015

3.565 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski