Amino acid dipepetide frequency for Anaerobacillus alkalilacustris

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.473AlaAla: 4.473 ± 0.087	0.566AlaCys: 0.566 ± 0.026	2.721AlaAsp: 2.721 ± 0.052	3.895AlaGlu: 3.895 ± 0.069	2.957AlaPhe: 2.957 ± 0.063	4.178AlaGly: 4.178 ± 0.077	1.167AlaHis: 1.167 ± 0.03	5.9AlaIle: 5.9 ± 0.085	4.517AlaLys: 4.517 ± 0.068	6.446AlaLeu: 6.446 ± 0.087	1.823AlaMet: 1.823 ± 0.042	2.949AlaAsn: 2.949 ± 0.058	1.8AlaPro: 1.8 ± 0.044	1.917AlaGln: 1.917 ± 0.048	2.4AlaArg: 2.4 ± 0.052	3.677AlaSer: 3.677 ± 0.066	3.457AlaThr: 3.457 ± 0.057	4.573AlaVal: 4.573 ± 0.075	0.562AlaTrp: 0.562 ± 0.022	2.094AlaTyr: 2.094 ± 0.041	0.001AlaXaa: 0.001 ± 0.001
Cys	0.415CysAla: 0.415 ± 0.021	0.106CysCys: 0.106 ± 0.01	0.413CysAsp: 0.413 ± 0.022	0.554CysGlu: 0.554 ± 0.024	0.376CysPhe: 0.376 ± 0.021	0.72CysGly: 0.72 ± 0.03	0.24CysHis: 0.24 ± 0.018	0.632CysIle: 0.632 ± 0.027	0.52CysLys: 0.52 ± 0.024	0.723CysLeu: 0.723 ± 0.026	0.19CysMet: 0.19 ± 0.014	0.396CysAsn: 0.396 ± 0.021	0.427CysPro: 0.427 ± 0.022	0.273CysGln: 0.273 ± 0.016	0.32CysArg: 0.32 ± 0.015	0.623CysSer: 0.623 ± 0.027	0.429CysThr: 0.429 ± 0.021	0.452CysVal: 0.452 ± 0.02	0.071CysTrp: 0.071 ± 0.008	0.311CysTyr: 0.311 ± 0.017	0.002CysXaa: 0.002 ± 0.002
Asp	2.891AspAla: 2.891 ± 0.057	0.426AspCys: 0.426 ± 0.023	2.444AspAsp: 2.444 ± 0.05	4.389AspGlu: 4.389 ± 0.072	2.499AspPhe: 2.499 ± 0.051	3.16AspGly: 3.16 ± 0.056	1.22AspHis: 1.22 ± 0.041	4.35AspIle: 4.35 ± 0.085	3.099AspLys: 3.099 ± 0.06	4.992AspLeu: 4.992 ± 0.072	1.193AspMet: 1.193 ± 0.037	1.884AspAsn: 1.884 ± 0.044	1.921AspPro: 1.921 ± 0.041	2.069AspGln: 2.069 ± 0.044	2.229AspArg: 2.229 ± 0.05	2.195AspSer: 2.195 ± 0.046	2.327AspThr: 2.327 ± 0.041	3.789AspVal: 3.789 ± 0.059	0.632AspTrp: 0.632 ± 0.023	2.155AspTyr: 2.155 ± 0.043	0.001AspXaa: 0.001 ± 0.001
Glu	5.091GluAla: 5.091 ± 0.078	0.457GluCys: 0.457 ± 0.022	3.688GluAsp: 3.688 ± 0.071	7.163GluGlu: 7.163 ± 0.115	2.673GluPhe: 2.673 ± 0.058	4.397GluGly: 4.397 ± 0.06	1.422GluHis: 1.422 ± 0.039	6.441GluIle: 6.441 ± 0.089	7.106GluLys: 7.106 ± 0.1	7.406GluLeu: 7.406 ± 0.097	2.269GluMet: 2.269 ± 0.044	4.117GluAsn: 4.117 ± 0.072	1.802GluPro: 1.802 ± 0.048	3.166GluGln: 3.166 ± 0.054	3.463GluArg: 3.463 ± 0.064	3.068GluSer: 3.068 ± 0.059	3.924GluThr: 3.924 ± 0.064	5.711GluVal: 5.711 ± 0.092	0.76GluTrp: 0.76 ± 0.028	2.257GluTyr: 2.257 ± 0.054	0.001GluXaa: 0.001 ± 0.001
Phe	2.689PheAla: 2.689 ± 0.048	0.383PheCys: 0.383 ± 0.02	2.431PheAsp: 2.431 ± 0.053	3.059PheGlu: 3.059 ± 0.049	2.514PhePhe: 2.514 ± 0.061	3.212PheGly: 3.212 ± 0.061	1.084PheHis: 1.084 ± 0.038	4.34PheIle: 4.34 ± 0.079	2.658PheLys: 2.658 ± 0.045	4.655PheLeu: 4.655 ± 0.094	1.119PheMet: 1.119 ± 0.035	2.061PheAsn: 2.061 ± 0.048	1.662PhePro: 1.662 ± 0.044	1.773PheGln: 1.773 ± 0.043	1.576PheArg: 1.576 ± 0.037	3.515PheSer: 3.515 ± 0.059	2.553PheThr: 2.553 ± 0.054	3.307PheVal: 3.307 ± 0.056	0.429PheTrp: 0.429 ± 0.02	1.728PheTyr: 1.728 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	4.389GlyAla: 4.389 ± 0.09	0.684GlyCys: 0.684 ± 0.025	3.083GlyAsp: 3.083 ± 0.059	4.319GlyGlu: 4.319 ± 0.071	3.327GlyPhe: 3.327 ± 0.071	4.458GlyGly: 4.458 ± 0.085	1.363GlyHis: 1.363 ± 0.042	5.896GlyIle: 5.896 ± 0.087	4.867GlyLys: 4.867 ± 0.074	6.212GlyLeu: 6.212 ± 0.1	1.91GlyMet: 1.91 ± 0.043	2.795GlyAsn: 2.795 ± 0.056	1.691GlyPro: 1.691 ± 0.047	2.034GlyGln: 2.034 ± 0.046	2.541GlyArg: 2.541 ± 0.059	3.62GlySer: 3.62 ± 0.07	3.781GlyThr: 3.781 ± 0.071	5.032GlyVal: 5.032 ± 0.072	0.693GlyTrp: 0.693 ± 0.027	2.702GlyTyr: 2.702 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	1.108HisAla: 1.108 ± 0.03	0.236HisCys: 0.236 ± 0.018	0.993HisAsp: 0.993 ± 0.038	1.333HisGlu: 1.333 ± 0.039	1.13HisPhe: 1.13 ± 0.033	1.33HisGly: 1.33 ± 0.037	0.604HisHis: 0.604 ± 0.024	1.564HisIle: 1.564 ± 0.041	1.119HisLys: 1.119 ± 0.036	2.197HisLeu: 2.197 ± 0.048	0.469HisMet: 0.469 ± 0.02	0.861HisAsn: 0.861 ± 0.029	1.088HisPro: 1.088 ± 0.035	0.827HisGln: 0.827 ± 0.026	0.877HisArg: 0.877 ± 0.031	1.264HisSer: 1.264 ± 0.037	1.052HisThr: 1.052 ± 0.035	1.348HisVal: 1.348 ± 0.037	0.205HisTrp: 0.205 ± 0.015	0.855HisTyr: 0.855 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.692IleAla: 5.692 ± 0.085	0.725IleCys: 0.725 ± 0.028	4.739IleAsp: 4.739 ± 0.074	6.719IleGlu: 6.719 ± 0.09	3.725IlePhe: 3.725 ± 0.08	6.066IleGly: 6.066 ± 0.098	1.683IleHis: 1.683 ± 0.041	7.054IleIle: 7.054 ± 0.11	5.403IleLys: 5.403 ± 0.086	7.422IleLeu: 7.422 ± 0.095	1.837IleMet: 1.837 ± 0.043	4.246IleAsn: 4.246 ± 0.069	3.392IlePro: 3.392 ± 0.069	2.818IleGln: 2.818 ± 0.056	3.125IleArg: 3.125 ± 0.063	5.795IleSer: 5.795 ± 0.076	4.736IleThr: 4.736 ± 0.078	6.141IleVal: 6.141 ± 0.083	0.663IleTrp: 0.663 ± 0.027	2.745IleTyr: 2.745 ± 0.057	0.0IleXaa: 0.0 ± 0.0
Lys	4.394LysAla: 4.394 ± 0.068	0.444LysCys: 0.444 ± 0.024	3.812LysAsp: 3.812 ± 0.071	7.197LysGlu: 7.197 ± 0.107	2.25LysPhe: 2.25 ± 0.044	4.553LysGly: 4.553 ± 0.064	1.402LysHis: 1.402 ± 0.036	5.421LysIle: 5.421 ± 0.069	6.569LysLys: 6.569 ± 0.1	6.404LysLeu: 6.404 ± 0.09	2.049LysMet: 2.049 ± 0.044	3.744LysAsn: 3.744 ± 0.067	2.089LysPro: 2.089 ± 0.049	3.31LysGln: 3.31 ± 0.07	3.332LysArg: 3.332 ± 0.052	3.796LysSer: 3.796 ± 0.061	3.601LysThr: 3.601 ± 0.057	5.215LysVal: 5.215 ± 0.084	0.773LysTrp: 0.773 ± 0.027	2.381LysTyr: 2.381 ± 0.053	0.003LysXaa: 0.003 ± 0.002
Leu	6.512LeuAla: 6.512 ± 0.096	0.786LeuCys: 0.786 ± 0.026	4.712LeuAsp: 4.712 ± 0.074	6.938LeuGlu: 6.938 ± 0.081	4.813LeuPhe: 4.813 ± 0.087	6.219LeuGly: 6.219 ± 0.09	1.893LeuHis: 1.893 ± 0.046	7.747LeuIle: 7.747 ± 0.11	7.033LeuLys: 7.033 ± 0.103	10.19LeuLeu: 10.19 ± 0.141	2.405LeuMet: 2.405 ± 0.054	4.746LeuAsn: 4.746 ± 0.072	3.737LeuPro: 3.737 ± 0.064	3.635LeuGln: 3.635 ± 0.063	3.744LeuArg: 3.744 ± 0.06	6.612LeuSer: 6.612 ± 0.093	5.73LeuThr: 5.73 ± 0.079	6.418LeuVal: 6.418 ± 0.093	0.785LeuTrp: 0.785 ± 0.029	3.136LeuTyr: 3.136 ± 0.062	0.005LeuXaa: 0.005 ± 0.002
Met	1.827MetAla: 1.827 ± 0.044	0.158MetCys: 0.158 ± 0.013	1.306MetAsp: 1.306 ± 0.035	1.795MetGlu: 1.795 ± 0.037	1.103MetPhe: 1.103 ± 0.034	1.585MetGly: 1.585 ± 0.046	0.382MetHis: 0.382 ± 0.019	2.142MetIle: 2.142 ± 0.05	2.437MetLys: 2.437 ± 0.055	2.402MetLeu: 2.402 ± 0.045	0.756MetMet: 0.756 ± 0.03	1.565MetAsn: 1.565 ± 0.034	0.909MetPro: 0.909 ± 0.033	0.789MetGln: 0.789 ± 0.026	0.983MetArg: 0.983 ± 0.03	1.593MetSer: 1.593 ± 0.039	1.523MetThr: 1.523 ± 0.039	1.829MetVal: 1.829 ± 0.05	0.18MetTrp: 0.18 ± 0.016	0.758MetTyr: 0.758 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	2.482AsnAla: 2.482 ± 0.048	0.417AsnCys: 0.417 ± 0.022	2.664AsnAsp: 2.664 ± 0.05	4.202AsnGlu: 4.202 ± 0.075	1.949AsnPhe: 1.949 ± 0.039	3.308AsnGly: 3.308 ± 0.059	1.171AsnHis: 1.171 ± 0.032	4.015AsnIle: 4.015 ± 0.068	3.515AsnLys: 3.515 ± 0.063	4.291AsnLeu: 4.291 ± 0.066	1.24AsnMet: 1.24 ± 0.037	2.577AsnAsn: 2.577 ± 0.052	2.129AsnPro: 2.129 ± 0.043	1.962AsnGln: 1.962 ± 0.042	2.146AsnArg: 2.146 ± 0.044	2.733AsnSer: 2.733 ± 0.054	2.287AsnThr: 2.287 ± 0.048	3.372AsnVal: 3.372 ± 0.064	0.545AsnTrp: 0.545 ± 0.026	1.93AsnTyr: 1.93 ± 0.043	0.001AsnXaa: 0.001 ± 0.001
Pro	1.75ProAla: 1.75 ± 0.044	0.245ProCys: 0.245 ± 0.016	1.714ProAsp: 1.714 ± 0.04	2.639ProGlu: 2.639 ± 0.054	1.969ProPhe: 1.969 ± 0.044	2.072ProGly: 2.072 ± 0.052	0.72ProHis: 0.72 ± 0.024	3.121ProIle: 3.121 ± 0.059	2.268ProLys: 2.268 ± 0.045	3.421ProLeu: 3.421 ± 0.068	0.794ProMet: 0.794 ± 0.031	1.964ProAsn: 1.964 ± 0.043	0.951ProPro: 0.951 ± 0.037	1.055ProGln: 1.055 ± 0.034	1.069ProArg: 1.069 ± 0.033	2.103ProSer: 2.103 ± 0.05	2.075ProThr: 2.075 ± 0.043	2.575ProVal: 2.575 ± 0.056	0.38ProTrp: 0.38 ± 0.02	1.443ProTyr: 1.443 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	2.199GlnAla: 2.199 ± 0.05	0.293GlnCys: 0.293 ± 0.018	1.474GlnAsp: 1.474 ± 0.041	2.758GlnGlu: 2.758 ± 0.061	1.76GlnPhe: 1.76 ± 0.041	2.109GlnGly: 2.109 ± 0.048	0.725GlnHis: 0.725 ± 0.031	2.891GlnIle: 2.891 ± 0.052	2.82GlnLys: 2.82 ± 0.057	4.183GlnLeu: 4.183 ± 0.078	1.079GlnMet: 1.079 ± 0.034	1.633GlnAsn: 1.633 ± 0.039	1.169GlnPro: 1.169 ± 0.038	1.684GlnGln: 1.684 ± 0.051	1.523GlnArg: 1.523 ± 0.043	2.084GlnSer: 2.084 ± 0.05	1.872GlnThr: 1.872 ± 0.042	2.407GlnVal: 2.407 ± 0.056	0.4GlnTrp: 0.4 ± 0.019	1.333GlnTyr: 1.333 ± 0.036	0.002GlnXaa: 0.002 ± 0.002
Arg	2.278ArgAla: 2.278 ± 0.051	0.288ArgCys: 0.288 ± 0.017	2.032ArgAsp: 2.032 ± 0.047	3.096ArgGlu: 3.096 ± 0.057	1.868ArgPhe: 1.868 ± 0.041	2.371ArgGly: 2.371 ± 0.05	0.741ArgHis: 0.741 ± 0.027	3.107ArgIle: 3.107 ± 0.059	3.296ArgLys: 3.296 ± 0.065	4.058ArgLeu: 4.058 ± 0.07	1.151ArgMet: 1.151 ± 0.035	2.042ArgAsn: 2.042 ± 0.047	1.262ArgPro: 1.262 ± 0.038	1.531ArgGln: 1.531 ± 0.041	1.748ArgArg: 1.748 ± 0.045	2.264ArgSer: 2.264 ± 0.053	2.035ArgThr: 2.035 ± 0.042	2.711ArgVal: 2.711 ± 0.055	0.413ArgTrp: 0.413 ± 0.021	1.571ArgTyr: 1.571 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	3.259SerAla: 3.259 ± 0.062	0.503SerCys: 0.503 ± 0.023	2.727SerAsp: 2.727 ± 0.05	4.157SerGlu: 4.157 ± 0.076	3.438SerPhe: 3.438 ± 0.06	3.971SerGly: 3.971 ± 0.065	1.194SerHis: 1.194 ± 0.033	5.472SerIle: 5.472 ± 0.076	4.079SerLys: 4.079 ± 0.071	6.088SerLeu: 6.088 ± 0.078	1.598SerMet: 1.598 ± 0.045	2.936SerAsn: 2.936 ± 0.062	1.972SerPro: 1.972 ± 0.05	1.932SerGln: 1.932 ± 0.045	2.218SerArg: 2.218 ± 0.047	3.86SerSer: 3.86 ± 0.07	3.124SerThr: 3.124 ± 0.055	4.148SerVal: 4.148 ± 0.06	0.624SerTrp: 0.624 ± 0.028	2.237SerTyr: 2.237 ± 0.045	0.003SerXaa: 0.003 ± 0.001
Thr	3.375ThrAla: 3.375 ± 0.063	0.414ThrCys: 0.414 ± 0.021	2.64ThrAsp: 2.64 ± 0.054	3.51ThrGlu: 3.51 ± 0.063	2.729ThrPhe: 2.729 ± 0.051	3.789ThrGly: 3.789 ± 0.067	0.995ThrHis: 0.995 ± 0.033	5.052ThrIle: 5.052 ± 0.08	3.655ThrLys: 3.655 ± 0.057	5.307ThrLeu: 5.307 ± 0.073	1.245ThrMet: 1.245 ± 0.034	2.847ThrAsn: 2.847 ± 0.058	2.164ThrPro: 2.164 ± 0.052	1.412ThrGln: 1.412 ± 0.036	1.958ThrArg: 1.958 ± 0.046	3.254ThrSer: 3.254 ± 0.065	3.033ThrThr: 3.033 ± 0.061	4.046ThrVal: 4.046 ± 0.06	0.48ThrTrp: 0.48 ± 0.024	1.943ThrTyr: 1.943 ± 0.046	0.003ThrXaa: 0.003 ± 0.002
Val	4.795ValAla: 4.795 ± 0.069	0.627ValCys: 0.627 ± 0.027	3.755ValAsp: 3.755 ± 0.064	5.278ValGlu: 5.278 ± 0.089	3.129ValPhe: 3.129 ± 0.064	4.87ValGly: 4.87 ± 0.073	1.362ValHis: 1.362 ± 0.035	6.238ValIle: 6.238 ± 0.084	4.873ValLys: 4.873 ± 0.071	6.677ValLeu: 6.677 ± 0.083	1.815ValMet: 1.815 ± 0.043	3.417ValAsn: 3.417 ± 0.06	2.527ValPro: 2.527 ± 0.054	2.298ValGln: 2.298 ± 0.053	2.702ValArg: 2.702 ± 0.058	4.646ValSer: 4.646 ± 0.071	4.153ValThr: 4.153 ± 0.074	5.348ValVal: 5.348 ± 0.092	0.595ValTrp: 0.595 ± 0.028	2.199ValTyr: 2.199 ± 0.045	0.003ValXaa: 0.003 ± 0.002
Trp	0.549TrpAla: 0.549 ± 0.023	0.081TrpCys: 0.081 ± 0.009	0.526TrpAsp: 0.526 ± 0.026	0.696TrpGlu: 0.696 ± 0.03	0.536TrpPhe: 0.536 ± 0.025	0.612TrpGly: 0.612 ± 0.029	0.209TrpHis: 0.209 ± 0.015	0.785TrpIle: 0.785 ± 0.031	0.721TrpLys: 0.721 ± 0.026	1.125TrpLeu: 1.125 ± 0.036	0.296TrpMet: 0.296 ± 0.018	0.51TrpAsn: 0.51 ± 0.024	0.232TrpPro: 0.232 ± 0.015	0.36TrpGln: 0.36 ± 0.022	0.374TrpArg: 0.374 ± 0.018	0.589TrpSer: 0.589 ± 0.023	0.41TrpThr: 0.41 ± 0.02	0.608TrpVal: 0.608 ± 0.025	0.148TrpTrp: 0.148 ± 0.013	0.333TrpTyr: 0.333 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.903TyrAla: 1.903 ± 0.044	0.353TyrCys: 0.353 ± 0.019	1.992TyrAsp: 1.992 ± 0.046	2.682TyrGlu: 2.682 ± 0.054	2.004TyrPhe: 2.004 ± 0.043	2.414TyrGly: 2.414 ± 0.049	0.884TyrHis: 0.884 ± 0.029	2.556TyrIle: 2.556 ± 0.054	2.181TyrLys: 2.181 ± 0.048	3.594TyrLeu: 3.594 ± 0.062	0.784TyrMet: 0.784 ± 0.025	1.646TyrAsn: 1.646 ± 0.045	1.402TyrPro: 1.402 ± 0.043	1.553TyrGln: 1.553 ± 0.037	1.533TyrArg: 1.533 ± 0.044	2.246TyrSer: 2.246 ± 0.047	1.744TyrThr: 1.744 ± 0.037	2.274TyrVal: 2.274 ± 0.047	0.364TyrTrp: 0.364 ± 0.021	1.545TyrTyr: 1.545 ± 0.043	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.001XaaAla: 0.001 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.003XaaIle: 0.003 ± 0.002	0.001XaaLys: 0.001 ± 0.001	0.002XaaLeu: 0.002 ± 0.001	0.002XaaMet: 0.002 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.002XaaPro: 0.002 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.003XaaSer: 0.003 ± 0.002	0.005XaaThr: 0.005 ± 0.002	0.003XaaVal: 0.003 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3502 proteins (1032631 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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