Lomentospora prolificans

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Microascales;

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8533 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N3N8L8|A0A2N3N8L8_9PEZI Iso_dh domain-containing protein OS=Lomentospora prolificans OX=41688 GN=jhhlp_003398 PE=3 SV=1
MM1 pKa = 7.64HH2 pKa = 6.92FTKK5 pKa = 10.65ALPILAAITPVILAEE20 pKa = 4.33IDD22 pKa = 4.02LDD24 pKa = 3.93NDD26 pKa = 5.5DD27 pKa = 5.76IPTQCTQVCDD37 pKa = 3.78PLLNLVRR44 pKa = 11.84QCNVDD49 pKa = 3.63DD50 pKa = 4.79DD51 pKa = 5.03AVGGDD56 pKa = 3.49RR57 pKa = 11.84NEE59 pKa = 4.62DD60 pKa = 3.45LLEE63 pKa = 4.23RR64 pKa = 11.84QCVCTNNSFDD74 pKa = 3.93VANVAALCASCMTQNVRR91 pKa = 11.84EE92 pKa = 4.08RR93 pKa = 11.84DD94 pKa = 3.8DD95 pKa = 5.58LEE97 pKa = 5.9DD98 pKa = 4.56INDD101 pKa = 4.43LMLACNFQSTSFAASATTIVQGVTVSATRR130 pKa = 11.84PTDD133 pKa = 3.28INQLTTTVGSEE144 pKa = 3.96PTSGSNNNNNNNNNNNDD161 pKa = 3.9DD162 pKa = 4.06GNGAGALAPGMVVSVAGAALACAMLLHH189 pKa = 6.92

Molecular weight: 19.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N3NEN5|A0A2N3NEN5_9PEZI Protein-lysine N-methyltransferase EFM5 OS=Lomentospora prolificans OX=41688 GN=EFM5 PE=3 SV=1
MM1 pKa = 7.45EE2 pKa = 4.95GWNPGVRR9 pKa = 11.84RR10 pKa = 11.84SNSRR14 pKa = 11.84SRR16 pKa = 11.84TSRR19 pKa = 11.84PTTPLRR25 pKa = 11.84PSSRR29 pKa = 11.84SSFRR33 pKa = 11.84EE34 pKa = 3.76SARR37 pKa = 11.84SSAQQDD43 pKa = 3.33APFPLNSFEE52 pKa = 4.18PAFAEE57 pKa = 4.81LADD60 pKa = 3.96SMADD64 pKa = 3.59LEE66 pKa = 4.6ANLMHH71 pKa = 6.64FQLMHH76 pKa = 6.55EE77 pKa = 4.21SLARR81 pKa = 11.84FSEE84 pKa = 4.57SFASFMYY91 pKa = 9.47GMNMNAFCVDD101 pKa = 4.58FPEE104 pKa = 4.34VWSFVALLQSPSWPSCWKK122 pKa = 8.98IGQVSFHH129 pKa = 7.15PCHH132 pKa = 6.7KK133 pKa = 10.22PRR135 pKa = 11.84DD136 pKa = 3.53KK137 pKa = 10.94RR138 pKa = 11.84IILKK142 pKa = 9.99SHH144 pKa = 7.01LLMPDD149 pKa = 3.08LRR151 pKa = 11.84FVTVTRR157 pKa = 11.84EE158 pKa = 3.35RR159 pKa = 11.84ARR161 pKa = 11.84ARR163 pKa = 11.84RR164 pKa = 11.84VGWRR168 pKa = 11.84DD169 pKa = 3.14NIYY172 pKa = 9.7DD173 pKa = 3.57YY174 pKa = 11.44RR175 pKa = 11.84RR176 pKa = 11.84IVCGQPQLSNAEE188 pKa = 4.09LKK190 pKa = 10.25ICNAGASGQASSGPRR205 pKa = 11.84HH206 pKa = 6.3SGHH209 pKa = 6.46ARR211 pKa = 11.84YY212 pKa = 9.51ARR214 pKa = 11.84RR215 pKa = 11.84KK216 pKa = 8.27GRR218 pKa = 11.84AFKK221 pKa = 10.99GII223 pKa = 3.67

Molecular weight: 25.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8533

0

8533

4365240

36

12958

511.6

56.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.889 ± 0.021

1.18 ± 0.01

5.825 ± 0.017

6.266 ± 0.026

3.684 ± 0.016

7.092 ± 0.02

2.271 ± 0.01

4.887 ± 0.017

4.862 ± 0.025

8.828 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.151 ± 0.009

3.586 ± 0.013

6.205 ± 0.03

3.783 ± 0.02

6.174 ± 0.022

7.993 ± 0.026

5.89 ± 0.017

6.286 ± 0.018

1.432 ± 0.01

2.717 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski