Phenylobacterium kunshanense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales; Caulobacteraceae; Phenylobacterium

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4047 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328BJ08|A0A328BJ08_9CAUL LprI domain-containing protein OS=Phenylobacterium kunshanense OX=1445034 GN=DJ019_05995 PE=4 SV=1
MM1 pKa = 7.69PFASATDD8 pKa = 3.65TAQAATDD15 pKa = 3.64IFAQGVRR22 pKa = 11.84PFGGEE27 pKa = 3.72APLGCACPVCRR38 pKa = 11.84GAVTVVEE45 pKa = 4.77DD46 pKa = 3.88GGEE49 pKa = 4.17GALQGYY55 pKa = 9.74LNADD59 pKa = 3.35QRR61 pKa = 11.84SGAVVNGKK69 pKa = 8.97QSFSIDD75 pKa = 3.22RR76 pKa = 11.84AGLQMTGFDD85 pKa = 4.87PDD87 pKa = 3.33TMQPYY92 pKa = 9.39PGWGGTAGQAFTVTYY107 pKa = 9.95AFRR110 pKa = 11.84SSAPFNMPSDD120 pKa = 3.68TEE122 pKa = 4.06GFQRR126 pKa = 11.84FNAQQIYY133 pKa = 9.09QAEE136 pKa = 4.15QALAAWSDD144 pKa = 3.54VANITFVRR152 pKa = 11.84VGSGAEE158 pKa = 3.99GEE160 pKa = 4.15EE161 pKa = 4.37AYY163 pKa = 11.16SNNAAILFSNYY174 pKa = 8.42TSGEE178 pKa = 4.25SGSSAFASYY187 pKa = 10.04PGSTAASSTAGDD199 pKa = 3.19VWINITAGSNSIPSMGNYY217 pKa = 7.96GAHH220 pKa = 5.43VLIHH224 pKa = 6.69EE225 pKa = 4.71IGHH228 pKa = 6.91AIGLAHH234 pKa = 7.03PGDD237 pKa = 4.39YY238 pKa = 11.02NSTADD243 pKa = 4.0GGAITYY249 pKa = 10.65SSDD252 pKa = 2.82AEE254 pKa = 4.36YY255 pKa = 11.5YY256 pKa = 10.46EE257 pKa = 4.41DD258 pKa = 3.55TRR260 pKa = 11.84QYY262 pKa = 10.95TVMSYY267 pKa = 10.54FSEE270 pKa = 4.7SNTGASYY277 pKa = 10.61GGRR280 pKa = 11.84YY281 pKa = 9.44ASAPQLDD288 pKa = 5.29DD289 pKa = 3.1IRR291 pKa = 11.84AAQIEE296 pKa = 4.67YY297 pKa = 8.86GANMTTRR304 pKa = 11.84TGDD307 pKa = 3.13TVYY310 pKa = 10.98GFNANAGRR318 pKa = 11.84DD319 pKa = 3.38WFAATSSSTRR329 pKa = 11.84LIFAVWDD336 pKa = 3.62AGGNDD341 pKa = 3.32TFDD344 pKa = 3.72FSGYY348 pKa = 8.77SQNQTIDD355 pKa = 3.2LRR357 pKa = 11.84EE358 pKa = 4.04GFFSNVGSLVGNVAVAQGAKK378 pKa = 9.6IEE380 pKa = 4.01NAIGGFGADD389 pKa = 4.01TINGNALANRR399 pKa = 11.84IAGNAGADD407 pKa = 3.93RR408 pKa = 11.84IFSGAGNDD416 pKa = 4.08TIEE419 pKa = 4.48GGAGSGYY426 pKa = 10.57LRR428 pKa = 11.84GEE430 pKa = 4.79DD431 pKa = 4.1GNDD434 pKa = 3.73SIIGGAAFDD443 pKa = 5.6DD444 pKa = 3.58IHH446 pKa = 7.7GNTGNDD452 pKa = 3.43TASGGDD458 pKa = 3.33GDD460 pKa = 4.18DD461 pKa = 3.26WVVGGKK467 pKa = 10.34DD468 pKa = 3.3NDD470 pKa = 3.87VLFGDD475 pKa = 3.87AGGDD479 pKa = 3.51VVFGNLGDD487 pKa = 3.97DD488 pKa = 3.99TVHH491 pKa = 6.6GGSGNDD497 pKa = 3.53TVRR500 pKa = 11.84GGQGNDD506 pKa = 3.22TLSGGDD512 pKa = 3.25GDD514 pKa = 5.68DD515 pKa = 3.66YY516 pKa = 11.8VSGDD520 pKa = 3.55RR521 pKa = 11.84GSDD524 pKa = 3.11TMTGGAGADD533 pKa = 3.72LFHH536 pKa = 6.89TSSDD540 pKa = 3.28AGIDD544 pKa = 3.61SVFDD548 pKa = 3.87FNLAEE553 pKa = 4.47GDD555 pKa = 4.04RR556 pKa = 11.84VLLDD560 pKa = 3.81PGTTFSVAQVGADD573 pKa = 3.45VVITLGSPSDD583 pKa = 3.56QMILVGIQLSSLTPGWIFGAA603 pKa = 4.2

Molecular weight: 61.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328BM32|A0A328BM32_9CAUL TSPc domain-containing protein OS=Phenylobacterium kunshanense OX=1445034 GN=DJ019_07480 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.47GFRR19 pKa = 11.84LRR21 pKa = 11.84MSTKK25 pKa = 10.02NGQKK29 pKa = 9.43ILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4047

0

4047

1261893

29

1949

311.8

33.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.127 ± 0.057

0.78 ± 0.013

5.811 ± 0.029

5.785 ± 0.035

3.502 ± 0.023

9.097 ± 0.04

1.809 ± 0.018

4.145 ± 0.027

2.983 ± 0.033

9.882 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.222 ± 0.018

2.226 ± 0.028

5.655 ± 0.032

3.048 ± 0.023

7.797 ± 0.043

4.819 ± 0.029

5.103 ± 0.031

7.642 ± 0.028

1.435 ± 0.015

2.133 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski