Mangrovibacterium marinum
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3633 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T5C0B1|A0A2T5C0B1_9BACT tRNA uridine(34) hydroxylase OS=Mangrovibacterium marinum OX=1639118 GN=trhO PE=3 SV=1
MM1 pKa = 7.03 KK2 pKa = 9.71 TIHH5 pKa = 7.24 KK6 pKa = 10.12 LFLLLAVVAFGACEE20 pKa = 4.13 GPMGPPGYY28 pKa = 10.22 DD29 pKa = 3.2 GLDD32 pKa = 3.71 GQDD35 pKa = 3.56 GQDD38 pKa = 3.26 GVNILGTVFEE48 pKa = 4.66 VNGDD52 pKa = 3.54 FTEE55 pKa = 4.23 NNQYY59 pKa = 8.53 TLYY62 pKa = 11.04 YY63 pKa = 9.21 PFPDD67 pKa = 4.31 DD68 pKa = 3.64 FQVYY72 pKa = 10.05 NGDD75 pKa = 3.51 AVVVYY80 pKa = 9.55 ILWKK84 pKa = 10.66 VVDD87 pKa = 5.05 GLDD90 pKa = 3.08 VWRR93 pKa = 11.84 ALPQTIFLEE102 pKa = 4.7 SGAFQYY108 pKa = 11.26 NFDD111 pKa = 3.96 YY112 pKa = 8.29 TTGDD116 pKa = 3.19 VQIFIDD122 pKa = 5.13 GEE124 pKa = 4.31 LDD126 pKa = 3.8 PAWLGSGDD134 pKa = 5.2 LVDD137 pKa = 3.38 QTFRR141 pKa = 11.84 IAVIPADD148 pKa = 4.01 LYY150 pKa = 10.68 SNKK153 pKa = 10.61 SIDD156 pKa = 3.11 ITNYY160 pKa = 10.8 DD161 pKa = 3.64 EE162 pKa = 4.45 VNRR165 pKa = 11.84 AMLLWGGKK173 pKa = 8.13 INRR176 pKa = 11.84 ATLVNSDD183 pKa = 3.67 LL184 pKa = 4.05
Molecular weight: 20.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.541
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.77
Rodwell 3.605
Grimsley 3.452
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.202
Thurlkill 3.617
EMBOSS 3.77
Sillero 3.91
Patrickios 1.036
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.808
Protein with the highest isoelectric point:
>tr|A0A2T5C0Y8|A0A2T5C0Y8_9BACT Gliding motility-associated protein GldM OS=Mangrovibacterium marinum OX=1639118 GN=C8N47_10913 PE=4 SV=1
MM1 pKa = 6.9 TRR3 pKa = 11.84 IVRR6 pKa = 11.84 LTTNYY11 pKa = 9.88 SRR13 pKa = 11.84 SRR15 pKa = 11.84 SHH17 pKa = 6.69 SVQSSPFGEE26 pKa = 4.3 LEE28 pKa = 4.03 GGFPSHH34 pKa = 6.79 PRR36 pKa = 11.84 DD37 pKa = 3.4 VGKK40 pKa = 8.64 CTKK43 pKa = 10.05 AANFEE48 pKa = 4.19 FPITNIQYY56 pKa = 10.54 RR57 pKa = 11.84 MRR59 pKa = 11.84 NRR61 pKa = 11.84 EE62 pKa = 3.72 TTLPQTPKK70 pKa = 10.78 GAA72 pKa = 3.69
Molecular weight: 8.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.677
IPC_protein 10.584
Toseland 10.643
ProMoST 10.394
Dawson 10.76
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 10.906
Grimsley 10.818
Solomon 10.891
Lehninger 10.862
Nozaki 10.643
DTASelect 10.482
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.687
Patrickios 10.716
IPC_peptide 10.891
IPC2_peptide 9.589
IPC2.peptide.svr19 8.523
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3633
0
3633
1323685
27
6431
364.4
40.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.52 ± 0.041
0.942 ± 0.017
5.757 ± 0.032
6.488 ± 0.04
4.832 ± 0.032
6.884 ± 0.038
1.845 ± 0.018
6.681 ± 0.036
6.301 ± 0.049
9.526 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.416 ± 0.024
5.073 ± 0.033
3.857 ± 0.024
4.036 ± 0.033
4.33 ± 0.031
6.297 ± 0.037
5.358 ± 0.066
6.49 ± 0.035
1.293 ± 0.017
4.077 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here