Amino acid dipepetide frequency for Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.124AlaAla: 17.124 ± 0.117
1.203AlaCys: 1.203 ± 0.023
6.488AlaAsp: 6.488 ± 0.056
6.861AlaGlu: 6.861 ± 0.066
4.423AlaPhe: 4.423 ± 0.046
10.179AlaGly: 10.179 ± 0.086
2.251AlaHis: 2.251 ± 0.031
6.685AlaIle: 6.685 ± 0.062
4.682AlaLys: 4.682 ± 0.052
12.715AlaLeu: 12.715 ± 0.077
3.567AlaMet: 3.567 ± 0.043
3.165AlaAsn: 3.165 ± 0.04
5.818AlaPro: 5.818 ± 0.059
4.001AlaGln: 4.001 ± 0.046
8.711AlaArg: 8.711 ± 0.07
7.189AlaSer: 7.189 ± 0.059
6.539AlaThr: 6.539 ± 0.063
8.663AlaVal: 8.663 ± 0.066
1.461AlaTrp: 1.461 ± 0.025
2.493AlaTyr: 2.493 ± 0.029
0.0AlaXaa: 0.0 ± 0.0
Cys
1.085CysAla: 1.085 ± 0.019
0.158CysCys: 0.158 ± 0.008
0.522CysAsp: 0.522 ± 0.015
0.46CysGlu: 0.46 ± 0.014
0.346CysPhe: 0.346 ± 0.009
0.978CysGly: 0.978 ± 0.022
0.255CysHis: 0.255 ± 0.011
0.42CysIle: 0.42 ± 0.012
0.234CysLys: 0.234 ± 0.008
0.821CysLeu: 0.821 ± 0.02
0.197CysMet: 0.197 ± 0.008
0.241CysAsn: 0.241 ± 0.009
0.462CysPro: 0.462 ± 0.014
0.243CysGln: 0.243 ± 0.01
0.763CysArg: 0.763 ± 0.02
0.555CysSer: 0.555 ± 0.016
0.456CysThr: 0.456 ± 0.014
0.661CysVal: 0.661 ± 0.017
0.127CysTrp: 0.127 ± 0.007
0.212CysTyr: 0.212 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
6.52AspAla: 6.52 ± 0.05
0.467AspCys: 0.467 ± 0.015
3.053AspAsp: 3.053 ± 0.041
3.119AspGlu: 3.119 ± 0.042
2.083AspPhe: 2.083 ± 0.028
4.87AspGly: 4.87 ± 0.057
1.258AspHis: 1.258 ± 0.025
2.949AspIle: 2.949 ± 0.037
1.936AspLys: 1.936 ± 0.032
5.459AspLeu: 5.459 ± 0.049
1.208AspMet: 1.208 ± 0.022
1.379AspAsn: 1.379 ± 0.027
3.241AspPro: 3.241 ± 0.038
1.739AspGln: 1.739 ± 0.028
4.141AspArg: 4.141 ± 0.04
2.239AspSer: 2.239 ± 0.034
2.49AspThr: 2.49 ± 0.04
4.23AspVal: 4.23 ± 0.044
0.855AspTrp: 0.855 ± 0.016
1.425AspTyr: 1.425 ± 0.022
0.0AspXaa: 0.0 ± 0.0
Glu
6.865GluAla: 6.865 ± 0.056
0.357GluCys: 0.357 ± 0.012
2.419GluAsp: 2.419 ± 0.032
2.614GluGlu: 2.614 ± 0.037
1.827GluPhe: 1.827 ± 0.024
3.722GluGly: 3.722 ± 0.04
1.173GluHis: 1.173 ± 0.021
3.377GluIle: 3.377 ± 0.04
2.149GluLys: 2.149 ± 0.032
5.294GluLeu: 5.294 ± 0.049
1.399GluMet: 1.399 ± 0.027
1.383GluAsn: 1.383 ± 0.026
2.561GluPro: 2.561 ± 0.035
2.134GluGln: 2.134 ± 0.028
4.668GluArg: 4.668 ± 0.057
2.324GluSer: 2.324 ± 0.035
3.094GluThr: 3.094 ± 0.038
3.668GluVal: 3.668 ± 0.044
0.623GluTrp: 0.623 ± 0.016
0.914GluTyr: 0.914 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
4.826PheAla: 4.826 ± 0.044
0.432PheCys: 0.432 ± 0.014
2.485PheAsp: 2.485 ± 0.031
2.052PheGlu: 2.052 ± 0.031
1.427PhePhe: 1.427 ± 0.027
3.737PheGly: 3.737 ± 0.042
0.74PheHis: 0.74 ± 0.016
1.629PheIle: 1.629 ± 0.026
1.128PheLys: 1.128 ± 0.021
3.287PheLeu: 3.287 ± 0.039
0.811PheMet: 0.811 ± 0.018
1.122PheAsn: 1.122 ± 0.022
1.651PhePro: 1.651 ± 0.026
1.035PheGln: 1.035 ± 0.023
2.302PheArg: 2.302 ± 0.032
2.228PheSer: 2.228 ± 0.03
2.034PheThr: 2.034 ± 0.034
3.045PheVal: 3.045 ± 0.035
0.523PheTrp: 0.523 ± 0.016
0.87PheTyr: 0.87 ± 0.019
0.0PheXaa: 0.0 ± 0.0
Gly
9.162GlyAla: 9.162 ± 0.073
0.881GlyCys: 0.881 ± 0.019
4.131GlyAsp: 4.131 ± 0.048
4.386GlyGlu: 4.386 ± 0.048
3.58GlyPhe: 3.58 ± 0.038
7.239GlyGly: 7.239 ± 0.089
1.893GlyHis: 1.893 ± 0.028
4.657GlyIle: 4.657 ± 0.046
3.444GlyLys: 3.444 ± 0.043
8.479GlyLeu: 8.479 ± 0.07
2.13GlyMet: 2.13 ± 0.027
2.339GlyAsn: 2.339 ± 0.048
3.565GlyPro: 3.565 ± 0.04
2.707GlyGln: 2.707 ± 0.034
5.952GlyArg: 5.952 ± 0.055
4.814GlySer: 4.814 ± 0.064
4.651GlyThr: 4.651 ± 0.068
5.998GlyVal: 5.998 ± 0.052
1.305GlyTrp: 1.305 ± 0.025
2.274GlyTyr: 2.274 ± 0.031
0.0GlyXaa: 0.0 ± 0.0
His
2.384HisAla: 2.384 ± 0.033
0.245HisCys: 0.245 ± 0.011
1.267HisAsp: 1.267 ± 0.024
0.988HisGlu: 0.988 ± 0.019
0.886HisPhe: 0.886 ± 0.019
1.97HisGly: 1.97 ± 0.036
0.623HisHis: 0.623 ± 0.021
0.935HisIle: 0.935 ± 0.018
0.522HisLys: 0.522 ± 0.014
2.063HisLeu: 2.063 ± 0.035
0.5HisMet: 0.5 ± 0.015
0.48HisAsn: 0.48 ± 0.012
1.314HisPro: 1.314 ± 0.025
0.635HisGln: 0.635 ± 0.015
1.541HisArg: 1.541 ± 0.027
1.013HisSer: 1.013 ± 0.02
0.843HisThr: 0.843 ± 0.019
1.485HisVal: 1.485 ± 0.025
0.342HisTrp: 0.342 ± 0.013
0.537HisTyr: 0.537 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
7.71IleAla: 7.71 ± 0.055
0.536IleCys: 0.536 ± 0.015
3.538IleAsp: 3.538 ± 0.036
3.443IleGlu: 3.443 ± 0.043
1.694IlePhe: 1.694 ± 0.028
5.115IleGly: 5.115 ± 0.049
0.902IleHis: 0.902 ± 0.019
2.247IleIle: 2.247 ± 0.028
1.724IleLys: 1.724 ± 0.023
4.227IleLeu: 4.227 ± 0.044
1.034IleMet: 1.034 ± 0.021
1.515IleAsn: 1.515 ± 0.026
2.375IlePro: 2.375 ± 0.032
1.205IleGln: 1.205 ± 0.023
3.213IleArg: 3.213 ± 0.033
3.139IleSer: 3.139 ± 0.037
2.748IleThr: 2.748 ± 0.041
4.516IleVal: 4.516 ± 0.038
0.61IleTrp: 0.61 ± 0.014
1.175IleTyr: 1.175 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
4.399LysAla: 4.399 ± 0.056
0.205LysCys: 0.205 ± 0.01
1.848LysAsp: 1.848 ± 0.032
1.685LysGlu: 1.685 ± 0.027
1.114LysPhe: 1.114 ± 0.026
2.704LysGly: 2.704 ± 0.035
0.685LysHis: 0.685 ± 0.017
1.981LysIle: 1.981 ± 0.03
1.523LysLys: 1.523 ± 0.034
3.798LysLeu: 3.798 ± 0.047
0.883LysMet: 0.883 ± 0.024
0.952LysAsn: 0.952 ± 0.019
2.408LysPro: 2.408 ± 0.036
1.224LysGln: 1.224 ± 0.022
2.628LysArg: 2.628 ± 0.031
2.195LysSer: 2.195 ± 0.034
2.124LysThr: 2.124 ± 0.03
2.57LysVal: 2.57 ± 0.041
0.441LysTrp: 0.441 ± 0.014
0.717LysTyr: 0.717 ± 0.017
0.0LysXaa: 0.0 ± 0.0
Leu
13.257LeuAla: 13.257 ± 0.083
0.926LeuCys: 0.926 ± 0.019
5.592LeuAsp: 5.592 ± 0.059
4.683LeuGlu: 4.683 ± 0.049
3.559LeuPhe: 3.559 ± 0.046
8.046LeuGly: 8.046 ± 0.064
1.877LeuHis: 1.877 ± 0.026
4.965LeuIle: 4.965 ± 0.045
3.815LeuLys: 3.815 ± 0.046
9.314LeuLeu: 9.314 ± 0.084
2.28LeuMet: 2.28 ± 0.03
2.718LeuAsn: 2.718 ± 0.037
5.444LeuPro: 5.444 ± 0.054
2.795LeuGln: 2.795 ± 0.035
6.982LeuArg: 6.982 ± 0.061
6.454LeuSer: 6.454 ± 0.05
5.527LeuThr: 5.527 ± 0.057
7.36LeuVal: 7.36 ± 0.063
1.092LeuTrp: 1.092 ± 0.021
1.912LeuTyr: 1.912 ± 0.027
0.0LeuXaa: 0.0 ± 0.0
Met
2.884MetAla: 2.884 ± 0.033
0.185MetCys: 0.185 ± 0.008
1.058MetAsp: 1.058 ± 0.02
0.994MetGlu: 0.994 ± 0.019
0.784MetPhe: 0.784 ± 0.019
1.653MetGly: 1.653 ± 0.026
0.453MetHis: 0.453 ± 0.013
1.387MetIle: 1.387 ± 0.025
1.023MetLys: 1.023 ± 0.02
2.504MetLeu: 2.504 ± 0.03
0.747MetMet: 0.747 ± 0.019
0.761MetAsn: 0.761 ± 0.018
1.539MetPro: 1.539 ± 0.024
0.893MetGln: 0.893 ± 0.017
1.934MetArg: 1.934 ± 0.028
1.75MetSer: 1.75 ± 0.023
1.851MetThr: 1.851 ± 0.028
1.687MetVal: 1.687 ± 0.029
0.233MetTrp: 0.233 ± 0.01
0.356MetTyr: 0.356 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
3.327AsnAla: 3.327 ± 0.042
0.271AsnCys: 0.271 ± 0.01
1.529AsnAsp: 1.529 ± 0.036
1.341AsnGlu: 1.341 ± 0.024
1.064AsnPhe: 1.064 ± 0.022
2.498AsnGly: 2.498 ± 0.044
0.52AsnHis: 0.52 ± 0.014
1.426AsnIle: 1.426 ± 0.03
0.847AsnLys: 0.847 ± 0.02
2.693AsnLeu: 2.693 ± 0.037
0.615AsnMet: 0.615 ± 0.017
0.836AsnAsn: 0.836 ± 0.022
1.79AsnPro: 1.79 ± 0.029
0.845AsnGln: 0.845 ± 0.016
1.825AsnArg: 1.825 ± 0.028
1.458AsnSer: 1.458 ± 0.029
1.353AsnThr: 1.353 ± 0.029
2.206AsnVal: 2.206 ± 0.036
0.442AsnTrp: 0.442 ± 0.015
0.71AsnTyr: 0.71 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
6.447ProAla: 6.447 ± 0.06
0.355ProCys: 0.355 ± 0.014
3.526ProAsp: 3.526 ± 0.036
3.206ProGlu: 3.206 ± 0.039
1.997ProPhe: 1.997 ± 0.032
4.346ProGly: 4.346 ± 0.041
1.082ProHis: 1.082 ± 0.021
2.436ProIle: 2.436 ± 0.034
2.041ProLys: 2.041 ± 0.03
4.646ProLeu: 4.646 ± 0.046
1.239ProMet: 1.239 ± 0.022
1.528ProAsn: 1.528 ± 0.026
2.959ProPro: 2.959 ± 0.056
1.771ProGln: 1.771 ± 0.028
3.277ProArg: 3.277 ± 0.04
3.114ProSer: 3.114 ± 0.045
2.691ProThr: 2.691 ± 0.035
4.085ProVal: 4.085 ± 0.047
0.691ProTrp: 0.691 ± 0.018
1.197ProTyr: 1.197 ± 0.021
0.0ProXaa: 0.0 ± 0.0
Gln
4.085GlnAla: 4.085 ± 0.041
0.254GlnCys: 0.254 ± 0.009
1.405GlnAsp: 1.405 ± 0.026
1.346GlnGlu: 1.346 ± 0.022
1.188GlnPhe: 1.188 ± 0.022
2.322GlnGly: 2.322 ± 0.027
0.686GlnHis: 0.686 ± 0.017
1.849GlnIle: 1.849 ± 0.029
1.137GlnLys: 1.137 ± 0.022
3.088GlnLeu: 3.088 ± 0.033
0.875GlnMet: 0.875 ± 0.017
0.908GlnAsn: 0.908 ± 0.02
1.834GlnPro: 1.834 ± 0.031
1.379GlnGln: 1.379 ± 0.025
2.559GlnArg: 2.559 ± 0.034
1.922GlnSer: 1.922 ± 0.03
1.739GlnThr: 1.739 ± 0.029
2.222GlnVal: 2.222 ± 0.027
0.427GlnTrp: 0.427 ± 0.012
0.638GlnTyr: 0.638 ± 0.016
0.0GlnXaa: 0.0 ± 0.0
Arg
8.011ArgAla: 8.011 ± 0.069
0.675ArgCys: 0.675 ± 0.019
4.049ArgAsp: 4.049 ± 0.042
4.113ArgGlu: 4.113 ± 0.049
2.802ArgPhe: 2.802 ± 0.034
4.983ArgGly: 4.983 ± 0.044
1.762ArgHis: 1.762 ± 0.028
4.02ArgIle: 4.02 ± 0.048
2.591ArgLys: 2.591 ± 0.038
7.674ArgLeu: 7.674 ± 0.06
1.848ArgMet: 1.848 ± 0.027
2.018ArgAsn: 2.018 ± 0.03
3.698ArgPro: 3.698 ± 0.044
2.558ArgGln: 2.558 ± 0.031
5.958ArgArg: 5.958 ± 0.068
4.202ArgSer: 4.202 ± 0.05
3.513ArgThr: 3.513 ± 0.039
4.68ArgVal: 4.68 ± 0.043
1.015ArgTrp: 1.015 ± 0.021
1.687ArgTyr: 1.687 ± 0.028
0.0ArgXaa: 0.0 ± 0.0
Ser
6.836SerAla: 6.836 ± 0.059
0.539SerCys: 0.539 ± 0.016
3.079SerAsp: 3.079 ± 0.038
2.954SerGlu: 2.954 ± 0.034
2.384SerPhe: 2.384 ± 0.028
5.7SerGly: 5.7 ± 0.061
1.174SerHis: 1.174 ± 0.022
2.982SerIle: 2.982 ± 0.036
1.868SerLys: 1.868 ± 0.029
5.649SerLeu: 5.649 ± 0.051
1.437SerMet: 1.437 ± 0.022
1.563SerAsn: 1.563 ± 0.032
3.1SerPro: 3.1 ± 0.036
1.77SerGln: 1.77 ± 0.025
4.067SerArg: 4.067 ± 0.043
3.643SerSer: 3.643 ± 0.051
2.994SerThr: 2.994 ± 0.045
4.272SerVal: 4.272 ± 0.047
0.809SerTrp: 0.809 ± 0.018
1.372SerTyr: 1.372 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
6.318ThrAla: 6.318 ± 0.059
0.447ThrCys: 0.447 ± 0.012
2.72ThrAsp: 2.72 ± 0.036
2.6ThrGlu: 2.6 ± 0.033
2.093ThrPhe: 2.093 ± 0.031
4.963ThrGly: 4.963 ± 0.06
1.015ThrHis: 1.015 ± 0.022
3.159ThrIle: 3.159 ± 0.048
1.698ThrLys: 1.698 ± 0.028
5.602ThrLeu: 5.602 ± 0.069
1.31ThrMet: 1.31 ± 0.022
1.474ThrAsn: 1.474 ± 0.036
3.253ThrPro: 3.253 ± 0.05
1.494ThrGln: 1.494 ± 0.027
3.452ThrArg: 3.452 ± 0.041
3.374ThrSer: 3.374 ± 0.041
3.16ThrThr: 3.16 ± 0.051
4.225ThrVal: 4.225 ± 0.052
0.67ThrTrp: 0.67 ± 0.019
1.226ThrTyr: 1.226 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
9.399ValAla: 9.399 ± 0.075
0.667ValCys: 0.667 ± 0.016
4.025ValAsp: 4.025 ± 0.04
4.12ValGlu: 4.12 ± 0.04
2.644ValPhe: 2.644 ± 0.034
5.668ValGly: 5.668 ± 0.051
1.421ValHis: 1.421 ± 0.023
3.964ValIle: 3.964 ± 0.043
2.567ValLys: 2.567 ± 0.037
7.433ValLeu: 7.433 ± 0.058
1.823ValMet: 1.823 ± 0.028
2.165ValAsn: 2.165 ± 0.031
3.763ValPro: 3.763 ± 0.035
2.169ValGln: 2.169 ± 0.031
4.894ValArg: 4.894 ± 0.052
4.414ValSer: 4.414 ± 0.045
4.585ValThr: 4.585 ± 0.062
6.075ValVal: 6.075 ± 0.054
0.891ValTrp: 0.891 ± 0.018
1.477ValTyr: 1.477 ± 0.027
0.0ValXaa: 0.0 ± 0.0
Trp
1.128TrpAla: 1.128 ± 0.022
0.148TrpCys: 0.148 ± 0.008
0.585TrpAsp: 0.585 ± 0.014
0.494TrpGlu: 0.494 ± 0.015
0.505TrpPhe: 0.505 ± 0.016
0.868TrpGly: 0.868 ± 0.02
0.34TrpHis: 0.34 ± 0.012
0.71TrpIle: 0.71 ± 0.016
0.493TrpLys: 0.493 ± 0.016
1.588TrpLeu: 1.588 ± 0.028
0.354TrpMet: 0.354 ± 0.012
0.447TrpAsn: 0.447 ± 0.013
0.736TrpPro: 0.736 ± 0.02
0.55TrpGln: 0.55 ± 0.016
1.212TrpArg: 1.212 ± 0.025
0.921TrpSer: 0.921 ± 0.019
0.791TrpThr: 0.791 ± 0.019
0.781TrpVal: 0.781 ± 0.019
0.222TrpTrp: 0.222 ± 0.01
0.292TrpTyr: 0.292 ± 0.01
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.449TyrAla: 2.449 ± 0.03
0.245TyrCys: 0.245 ± 0.009
1.345TyrAsp: 1.345 ± 0.023
1.106TyrGlu: 1.106 ± 0.02
0.966TyrPhe: 0.966 ± 0.023
2.027TyrGly: 2.027 ± 0.027
0.464TyrHis: 0.464 ± 0.012
0.886TyrIle: 0.886 ± 0.02
0.664TyrLys: 0.664 ± 0.015
2.261TyrLeu: 2.261 ± 0.033
0.417TyrMet: 0.417 ± 0.013
0.603TyrAsn: 0.603 ± 0.018
1.123TyrPro: 1.123 ± 0.022
0.72TyrGln: 0.72 ± 0.016
1.776TyrArg: 1.776 ± 0.027
1.244TyrSer: 1.244 ± 0.023
1.069TyrThr: 1.069 ± 0.024
1.735TyrVal: 1.735 ± 0.027
0.385TyrTrp: 0.385 ± 0.013
0.602TyrTyr: 0.602 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8253 proteins (2616478 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski