Clostridium collagenovorans DSM 3089
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2997 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5U0E8|A0A1M5U0E8_9CLOT Shikimate dehydrogenase (NADP(+)) OS=Clostridium collagenovorans DSM 3089 OX=1121306 GN=aroE PE=3 SV=1
MM1 pKa = 7.51 INKK4 pKa = 9.56 KK5 pKa = 10.35 EE6 pKa = 4.03 IIEE9 pKa = 4.13 YY10 pKa = 10.68 LSDD13 pKa = 3.71 FQLEE17 pKa = 4.53 CIKK20 pKa = 10.24 EE21 pKa = 4.05 IKK23 pKa = 9.84 VEE25 pKa = 3.77 KK26 pKa = 10.5 DD27 pKa = 3.11 LIIFKK32 pKa = 10.84 FEE34 pKa = 4.41 LEE36 pKa = 4.5 LEE38 pKa = 4.31 DD39 pKa = 5.07 SEE41 pKa = 5.47 IEE43 pKa = 4.03 AAEE46 pKa = 3.96 AFADD50 pKa = 4.4 EE51 pKa = 4.61 EE52 pKa = 4.57 CEE54 pKa = 4.0 EE55 pKa = 4.23 PKK57 pKa = 10.62 KK58 pKa = 10.81 SEE60 pKa = 3.52 EE61 pKa = 3.97 WYY63 pKa = 10.06 EE64 pKa = 4.04 EE65 pKa = 3.85 HH66 pKa = 6.81 FLPYY70 pKa = 10.23 LSDD73 pKa = 3.43 MAIDD77 pKa = 4.37 DD78 pKa = 4.37 VEE80 pKa = 4.72 EE81 pKa = 4.46 MIQEE85 pKa = 4.24 CAEE88 pKa = 4.11 EE89 pKa = 4.22 FDD91 pKa = 5.26 LDD93 pKa = 3.99 YY94 pKa = 11.25 EE95 pKa = 5.41 LIMLDD100 pKa = 3.31 ISEE103 pKa = 4.64 EE104 pKa = 4.29 KK105 pKa = 10.23 YY106 pKa = 11.15 DD107 pKa = 4.0 SIEE110 pKa = 3.73 FLGAFLCNGKK120 pKa = 9.09 EE121 pKa = 4.25 VYY123 pKa = 10.4 LEE125 pKa = 4.22 EE126 pKa = 4.69 YY127 pKa = 9.74 ISEE130 pKa = 4.5 III132 pKa = 4.26
Molecular weight: 15.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.846
IPC_protein 3.783
Toseland 3.617
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.592
Rodwell 3.617
Grimsley 3.528
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 3.948
Thurlkill 3.63
EMBOSS 3.605
Sillero 3.897
Patrickios 1.812
IPC_peptide 3.732
IPC2_peptide 3.884
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|A0A1M5YMP2|A0A1M5YMP2_9CLOT Y_Y_Y domain-containing protein OS=Clostridium collagenovorans DSM 3089 OX=1121306 GN=SAMN02745196_03063 PE=4 SV=1
MM1 pKa = 7.77 KK2 pKa = 8.13 MTLQPKK8 pKa = 9.15 KK9 pKa = 8.69 RR10 pKa = 11.84 QRR12 pKa = 11.84 NKK14 pKa = 8.71 EE15 pKa = 3.51 HH16 pKa = 6.78 GFRR19 pKa = 11.84 KK20 pKa = 9.98 RR21 pKa = 11.84 MSTASGRR28 pKa = 11.84 NVLKK32 pKa = 10.3 RR33 pKa = 11.84 RR34 pKa = 11.84 KK35 pKa = 9.06 RR36 pKa = 11.84 KK37 pKa = 8.68 GRR39 pKa = 11.84 KK40 pKa = 9.1 RR41 pKa = 11.84 LTAA44 pKa = 4.18
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.407
IPC2_protein 10.877
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.427
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.149
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.037
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2997
0
2997
939149
39
3255
313.4
35.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.449 ± 0.043
1.261 ± 0.02
5.421 ± 0.036
7.862 ± 0.056
4.392 ± 0.035
6.316 ± 0.041
1.292 ± 0.017
9.788 ± 0.061
9.024 ± 0.046
9.33 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.65 ± 0.023
6.242 ± 0.048
2.701 ± 0.025
2.203 ± 0.019
3.272 ± 0.029
6.572 ± 0.037
4.861 ± 0.044
6.652 ± 0.038
0.621 ± 0.012
4.092 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here