Tyzzerella sp. An114

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Tyzzerella; unclassified Tyzzerella

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2549 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4UR18|A0A1Y4UR18_9FIRM PadR family transcriptional regulator OS=Tyzzerella sp. An114 OX=1965545 GN=B5E58_00040 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.4KK3 pKa = 10.41SILLLSIMFLVMLSGCNTNTDD24 pKa = 3.22NTDD27 pKa = 3.25EE28 pKa = 4.24VEE30 pKa = 4.27KK31 pKa = 10.86NQEE34 pKa = 3.71NTTDD38 pKa = 3.19ISEE41 pKa = 4.68EE42 pKa = 3.53IAGYY46 pKa = 10.38IFIDD50 pKa = 3.75GDD52 pKa = 3.59KK53 pKa = 10.8LYY55 pKa = 10.87VDD57 pKa = 3.65EE58 pKa = 5.62VEE60 pKa = 5.27IIEE63 pKa = 4.61NSDD66 pKa = 3.54KK67 pKa = 11.23EE68 pKa = 4.27RR69 pKa = 11.84MEE71 pKa = 4.12EE72 pKa = 4.51LNINDD77 pKa = 3.48SDD79 pKa = 4.11FPNGYY84 pKa = 10.04YY85 pKa = 9.86IYY87 pKa = 10.64NPTFEE92 pKa = 4.94ADD94 pKa = 3.31EE95 pKa = 4.19YY96 pKa = 11.41KK97 pKa = 9.54ITDD100 pKa = 3.24DD101 pKa = 3.32TAYY104 pKa = 10.95DD105 pKa = 3.66FVDD108 pKa = 4.21FNLYY112 pKa = 10.72YY113 pKa = 9.8IDD115 pKa = 4.38EE116 pKa = 4.55NEE118 pKa = 3.82ADD120 pKa = 3.22GDD122 pKa = 3.57RR123 pKa = 11.84RR124 pKa = 11.84YY125 pKa = 9.23RR126 pKa = 11.84TNNIDD131 pKa = 3.54EE132 pKa = 5.07FIKK135 pKa = 10.41GSSYY139 pKa = 10.88RR140 pKa = 11.84EE141 pKa = 3.95EE142 pKa = 4.9LPLEE146 pKa = 4.19QQTIPYY152 pKa = 9.41IIEE155 pKa = 3.77LHH157 pKa = 6.54GEE159 pKa = 3.74DD160 pKa = 4.21VYY162 pKa = 11.27CIKK165 pKa = 10.93EE166 pKa = 4.01EE167 pKa = 4.37LLYY170 pKa = 9.92TQQ172 pKa = 4.65

Molecular weight: 20.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4UQ75|A0A1Y4UQ75_9FIRM Citrate synthase OS=Tyzzerella sp. An114 OX=1965545 GN=B5E58_04525 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.81VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2549

0

2549

804866

37

2606

315.8

35.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.987 ± 0.053

1.341 ± 0.019

5.906 ± 0.039

7.681 ± 0.05

4.602 ± 0.036

6.723 ± 0.051

1.317 ± 0.019

9.547 ± 0.065

8.367 ± 0.05

7.726 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.862 ± 0.024

5.975 ± 0.043

2.925 ± 0.028

2.238 ± 0.023

3.417 ± 0.037

6.256 ± 0.046

5.389 ± 0.049

6.802 ± 0.036

0.699 ± 0.016

4.24 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski