Bacteroidales bacterium KHT7

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; unclassified Bacteroidales

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3157 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G7TIF1|A0A1G7TIF1_9BACT Ankyrin repeat-containing protein OS=Bacteroidales bacterium KHT7 OX=1855373 GN=SAMN05216518_12736 PE=4 SV=1
MM1 pKa = 8.18DD2 pKa = 6.18DD3 pKa = 3.36SVFDD7 pKa = 3.63VRR9 pKa = 11.84YY10 pKa = 10.08DD11 pKa = 3.66DD12 pKa = 5.6NYY14 pKa = 11.06TEE16 pKa = 5.66LDD18 pKa = 3.51FEE20 pKa = 4.87KK21 pKa = 11.0YY22 pKa = 9.61FVISHH27 pKa = 6.94KK28 pKa = 10.71LALTEE33 pKa = 3.52VSYY36 pKa = 11.09YY37 pKa = 10.12IFEE40 pKa = 4.53KK41 pKa = 10.16STGKK45 pKa = 9.95NVFDD49 pKa = 5.74GYY51 pKa = 11.46DD52 pKa = 3.54CFISNIDD59 pKa = 3.89EE60 pKa = 4.13LTEE63 pKa = 4.37NNDD66 pKa = 3.43SLLLCVCLPEE76 pKa = 6.14KK77 pKa = 10.86DD78 pKa = 3.78YY79 pKa = 11.47FDD81 pKa = 4.23YY82 pKa = 11.37KK83 pKa = 11.16LFLCDD88 pKa = 3.52LSKK91 pKa = 11.24DD92 pKa = 3.23IFQEE96 pKa = 4.04LNIDD100 pKa = 3.86DD101 pKa = 4.72FFSEE105 pKa = 4.5TSDD108 pKa = 3.58MPPFIADD115 pKa = 3.99CFRR118 pKa = 11.84LQKK121 pKa = 9.67TDD123 pKa = 3.12NDD125 pKa = 4.37SVVITYY131 pKa = 10.9NNDD134 pKa = 3.23NQCVKK139 pKa = 10.73QKK141 pKa = 10.47IALKK145 pKa = 7.88YY146 pKa = 8.29TRR148 pKa = 4.54

Molecular weight: 17.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G7HRL9|A0A1G7HRL9_9BACT RHS repeat-associated core domain-containing protein OS=Bacteroidales bacterium KHT7 OX=1855373 GN=SAMN05216518_102103 PE=4 SV=1
MM1 pKa = 7.91PSGKK5 pKa = 9.66KK6 pKa = 9.7KK7 pKa = 9.82KK8 pKa = 7.49RR9 pKa = 11.84HH10 pKa = 5.6KK11 pKa = 10.32MSTHH15 pKa = 5.04KK16 pKa = 10.3RR17 pKa = 11.84KK18 pKa = 9.87KK19 pKa = 8.77RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3157

0

3157

1128700

29

3616

357.5

40.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.078 ± 0.041

1.535 ± 0.019

5.892 ± 0.027

6.278 ± 0.048

4.733 ± 0.03

6.722 ± 0.044

1.824 ± 0.02

6.88 ± 0.04

7.213 ± 0.043

8.4 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.823 ± 0.022

5.564 ± 0.041

3.404 ± 0.023

3.17 ± 0.022

4.256 ± 0.032

6.391 ± 0.041

5.455 ± 0.035

6.664 ± 0.035

1.242 ± 0.016

4.474 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski