Siphoviridae sp.

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345BPV8|A0A345BPV8_9CAUD Uncharacterized protein OS=Siphoviridae sp. OX=2170413 PE=4 SV=1
MM1 pKa = 7.24RR2 pKa = 11.84TSFSTIYY9 pKa = 10.8DD10 pKa = 3.66SFLSKK15 pKa = 9.39ITSDD19 pKa = 3.33MYY21 pKa = 10.84MEE23 pKa = 5.4LDD25 pKa = 3.28EE26 pKa = 5.2LQTFRR31 pKa = 11.84LLQEE35 pKa = 4.4LLIDD39 pKa = 5.16AIPWFEE45 pKa = 4.3FPRR48 pKa = 11.84KK49 pKa = 9.51NLNDD53 pKa = 3.64YY54 pKa = 10.58EE55 pKa = 4.32LLIDD59 pKa = 4.89KK60 pKa = 10.75YY61 pKa = 11.5DD62 pKa = 3.62DD63 pKa = 3.87VYY65 pKa = 11.52CGVEE69 pKa = 3.55TDD71 pKa = 3.59MQDD74 pKa = 3.14VEE76 pKa = 4.61IIVYY80 pKa = 9.73EE81 pKa = 4.01GGYY84 pKa = 10.64FNVDD88 pKa = 3.15LTLEE92 pKa = 4.46EE93 pKa = 4.63INILSCYY100 pKa = 9.78MIVEE104 pKa = 4.22WLGQQLASIEE114 pKa = 4.38NIRR117 pKa = 11.84MKK119 pKa = 10.99YY120 pKa = 9.92SGSDD124 pKa = 2.92

Molecular weight: 14.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345BPP9|A0A345BPP9_9CAUD Uncharacterized protein OS=Siphoviridae sp. OX=2170413 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.27KK3 pKa = 10.11IIVLMVVAICFIFTAKK19 pKa = 9.44IVKK22 pKa = 10.12AEE24 pKa = 3.81PHH26 pKa = 6.18LKK28 pKa = 9.23YY29 pKa = 10.25KK30 pKa = 10.6QYY32 pKa = 10.76FKK34 pKa = 11.32EE35 pKa = 4.1DD36 pKa = 3.31MRR38 pKa = 11.84GTDD41 pKa = 3.41LTKK44 pKa = 11.15AKK46 pKa = 9.77IKK48 pKa = 10.4RR49 pKa = 11.84INRR52 pKa = 11.84YY53 pKa = 8.98IFNHH57 pKa = 5.42EE58 pKa = 4.01FKK60 pKa = 10.93ANKK63 pKa = 9.34IYY65 pKa = 10.7RR66 pKa = 11.84LRR68 pKa = 11.84GYY70 pKa = 10.48DD71 pKa = 3.36VIFITNRR78 pKa = 11.84KK79 pKa = 8.73GRR81 pKa = 11.84EE82 pKa = 3.99KK83 pKa = 10.21SWKK86 pKa = 10.07LLEE89 pKa = 4.22TRR91 pKa = 11.84KK92 pKa = 10.41KK93 pKa = 10.04PVIILSIGRR102 pKa = 11.84MYY104 pKa = 10.9NYY106 pKa = 10.48NGDD109 pKa = 3.93GQCTDD114 pKa = 3.02GTYY117 pKa = 9.7TGYY120 pKa = 10.94RR121 pKa = 11.84YY122 pKa = 5.91TTKK125 pKa = 10.1RR126 pKa = 11.84GKK128 pKa = 9.41RR129 pKa = 11.84AVMRR133 pKa = 11.84FPLDD137 pKa = 3.69TIIKK141 pKa = 7.9TVEE144 pKa = 3.84IFPGTNQYY152 pKa = 9.83TDD154 pKa = 3.35TVAYY158 pKa = 10.36RR159 pKa = 11.84SDD161 pKa = 3.18TCLDD165 pKa = 3.1IKK167 pKa = 11.12

Molecular weight: 19.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

115

0

115

29956

80

2706

260.5

29.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.707 ± 0.393

1.325 ± 0.178

6.386 ± 0.235

8.312 ± 0.362

4.149 ± 0.221

5.712 ± 0.246

1.392 ± 0.125

8.689 ± 0.294

9.504 ± 0.331

8.038 ± 0.156

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.367 ± 0.138

7.207 ± 0.181

2.634 ± 0.189

3.178 ± 0.244

3.806 ± 0.132

6.399 ± 0.275

5.097 ± 0.23

4.971 ± 0.151

1.025 ± 0.078

5.101 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski