Amino acid dipepetide frequency for Siphoviridae sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.804AlaAla: 2.804 ± 0.63
0.534AlaCys: 0.534 ± 0.149
2.971AlaAsp: 2.971 ± 0.536
4.306AlaGlu: 4.306 ± 0.61
1.803AlaPhe: 1.803 ± 0.233
2.637AlaGly: 2.637 ± 0.304
0.601AlaHis: 0.601 ± 0.138
3.973AlaIle: 3.973 ± 0.435
4.006AlaLys: 4.006 ± 0.433
4.34AlaLeu: 4.34 ± 0.582
1.235AlaMet: 1.235 ± 0.211
3.405AlaAsn: 3.405 ± 0.339
0.968AlaPro: 0.968 ± 0.178
2.17AlaGln: 2.17 ± 0.373
1.836AlaArg: 1.836 ± 0.31
2.871AlaSer: 2.871 ± 0.382
2.237AlaThr: 2.237 ± 0.35
2.303AlaVal: 2.303 ± 0.326
0.467AlaTrp: 0.467 ± 0.125
1.602AlaTyr: 1.602 ± 0.193
0.0AlaXaa: 0.0 ± 0.0
Cys
0.534CysAla: 0.534 ± 0.128
0.367CysCys: 0.367 ± 0.144
0.935CysAsp: 0.935 ± 0.198
0.868CysGlu: 0.868 ± 0.18
0.701CysPhe: 0.701 ± 0.167
1.269CysGly: 1.269 ± 0.317
0.2CysHis: 0.2 ± 0.086
0.601CysIle: 0.601 ± 0.148
1.168CysLys: 1.168 ± 0.261
1.102CysLeu: 1.102 ± 0.195
0.267CysMet: 0.267 ± 0.099
0.835CysAsn: 0.835 ± 0.206
0.634CysPro: 0.634 ± 0.16
0.3CysGln: 0.3 ± 0.101
0.668CysArg: 0.668 ± 0.187
0.901CysSer: 0.901 ± 0.231
0.434CysThr: 0.434 ± 0.113
0.701CysVal: 0.701 ± 0.144
0.134CysTrp: 0.134 ± 0.062
0.634CysTyr: 0.634 ± 0.147
0.0CysXaa: 0.0 ± 0.0
Asp
2.971AspAla: 2.971 ± 0.533
0.901AspCys: 0.901 ± 0.217
3.038AspAsp: 3.038 ± 0.351
4.807AspGlu: 4.807 ± 0.434
3.438AspPhe: 3.438 ± 0.393
3.973AspGly: 3.973 ± 0.492
0.534AspHis: 0.534 ± 0.131
6.41AspIle: 6.41 ± 0.306
6.109AspLys: 6.109 ± 0.492
5.208AspLeu: 5.208 ± 0.454
2.103AspMet: 2.103 ± 0.314
3.605AspAsn: 3.605 ± 0.313
1.669AspPro: 1.669 ± 0.215
1.369AspGln: 1.369 ± 0.26
2.637AspArg: 2.637 ± 0.211
4.073AspSer: 4.073 ± 0.455
3.338AspThr: 3.338 ± 0.348
3.138AspVal: 3.138 ± 0.296
0.668AspTrp: 0.668 ± 0.152
3.872AspTyr: 3.872 ± 0.457
0.0AspXaa: 0.0 ± 0.0
Glu
3.839GluAla: 3.839 ± 0.609
1.002GluCys: 1.002 ± 0.189
5.074GluAsp: 5.074 ± 0.515
8.446GluGlu: 8.446 ± 0.796
2.971GluPhe: 2.971 ± 0.419
4.607GluGly: 4.607 ± 0.371
0.968GluHis: 0.968 ± 0.183
7.511GluIle: 7.511 ± 0.485
8.68GluLys: 8.68 ± 0.687
7.478GluLeu: 7.478 ± 0.623
2.203GluMet: 2.203 ± 0.258
6.243GluAsn: 6.243 ± 0.443
1.703GluPro: 1.703 ± 0.299
2.771GluGln: 2.771 ± 0.393
2.671GluArg: 2.671 ± 0.358
4.24GluSer: 4.24 ± 0.544
3.438GluThr: 3.438 ± 0.344
4.407GluVal: 4.407 ± 0.531
1.202GluTrp: 1.202 ± 0.232
3.672GluTyr: 3.672 ± 0.418
0.0GluXaa: 0.0 ± 0.0
Phe
1.636PheAla: 1.636 ± 0.253
0.601PheCys: 0.601 ± 0.158
2.771PheAsp: 2.771 ± 0.333
2.971PheGlu: 2.971 ± 0.325
1.602PhePhe: 1.602 ± 0.214
2.003PheGly: 2.003 ± 0.287
0.334PheHis: 0.334 ± 0.123
4.106PheIle: 4.106 ± 0.507
3.272PheLys: 3.272 ± 0.333
3.272PheLeu: 3.272 ± 0.36
1.002PheMet: 1.002 ± 0.199
3.539PheAsn: 3.539 ± 0.324
1.669PhePro: 1.669 ± 0.254
1.402PheGln: 1.402 ± 0.213
1.569PheArg: 1.569 ± 0.191
3.205PheSer: 3.205 ± 0.39
2.27PheThr: 2.27 ± 0.325
1.936PheVal: 1.936 ± 0.256
0.134PheTrp: 0.134 ± 0.07
2.203PheTyr: 2.203 ± 0.337
0.0PheXaa: 0.0 ± 0.0
Gly
2.504GlyAla: 2.504 ± 0.336
0.868GlyCys: 0.868 ± 0.253
2.971GlyAsp: 2.971 ± 0.38
4.707GlyGlu: 4.707 ± 0.403
2.804GlyPhe: 2.804 ± 0.382
3.338GlyGly: 3.338 ± 0.498
0.734GlyHis: 0.734 ± 0.132
5.208GlyIle: 5.208 ± 0.477
5.308GlyLys: 5.308 ± 0.396
4.373GlyLeu: 4.373 ± 0.407
1.569GlyMet: 1.569 ± 0.184
3.639GlyAsn: 3.639 ± 0.407
0.701GlyPro: 0.701 ± 0.152
1.703GlyGln: 1.703 ± 0.255
2.303GlyArg: 2.303 ± 0.256
3.572GlySer: 3.572 ± 0.388
4.106GlyThr: 4.106 ± 0.747
2.604GlyVal: 2.604 ± 0.331
0.768GlyTrp: 0.768 ± 0.159
3.338GlyTyr: 3.338 ± 0.388
0.0GlyXaa: 0.0 ± 0.0
His
0.401HisAla: 0.401 ± 0.116
0.234HisCys: 0.234 ± 0.101
0.734HisAsp: 0.734 ± 0.153
1.202HisGlu: 1.202 ± 0.226
0.801HisPhe: 0.801 ± 0.173
0.801HisGly: 0.801 ± 0.196
0.267HisHis: 0.267 ± 0.114
1.202HisIle: 1.202 ± 0.187
1.002HisLys: 1.002 ± 0.177
1.269HisLeu: 1.269 ± 0.219
0.067HisMet: 0.067 ± 0.046
1.035HisAsn: 1.035 ± 0.185
0.601HisPro: 0.601 ± 0.148
0.434HisGln: 0.434 ± 0.104
0.701HisArg: 0.701 ± 0.15
1.035HisSer: 1.035 ± 0.168
0.601HisThr: 0.601 ± 0.131
0.501HisVal: 0.501 ± 0.132
0.1HisTrp: 0.1 ± 0.067
0.935HisTyr: 0.935 ± 0.215
0.0HisXaa: 0.0 ± 0.0
Ile
4.039IleAla: 4.039 ± 0.388
1.269IleCys: 1.269 ± 0.233
6.51IleAsp: 6.51 ± 0.479
6.844IleGlu: 6.844 ± 0.558
3.005IlePhe: 3.005 ± 0.43
4.373IleGly: 4.373 ± 0.449
1.335IleHis: 1.335 ± 0.251
6.71IleIle: 6.71 ± 0.657
9.715IleLys: 9.715 ± 0.627
6.41IleLeu: 6.41 ± 0.657
1.636IleMet: 1.636 ± 0.293
6.309IleAsn: 6.309 ± 0.466
3.038IlePro: 3.038 ± 0.402
3.005IleGln: 3.005 ± 0.338
3.238IleArg: 3.238 ± 0.329
5.942IleSer: 5.942 ± 0.548
4.14IleThr: 4.14 ± 0.44
4.774IleVal: 4.774 ± 0.426
0.734IleTrp: 0.734 ± 0.159
3.872IleTyr: 3.872 ± 0.384
0.0IleXaa: 0.0 ± 0.0
Lys
4.707LysAla: 4.707 ± 0.458
1.235LysCys: 1.235 ± 0.285
6.844LysAsp: 6.844 ± 0.477
10.482LysGlu: 10.482 ± 0.645
3.238LysPhe: 3.238 ± 0.287
5.141LysGly: 5.141 ± 0.426
1.102LysHis: 1.102 ± 0.181
8.78LysIle: 8.78 ± 0.755
9.548LysLys: 9.548 ± 0.815
7.044LysLeu: 7.044 ± 0.39
2.404LysMet: 2.404 ± 0.345
6.143LysAsn: 6.143 ± 0.491
1.97LysPro: 1.97 ± 0.266
2.337LysGln: 2.337 ± 0.236
3.372LysArg: 3.372 ± 0.481
4.807LysSer: 4.807 ± 0.507
4.674LysThr: 4.674 ± 0.408
4.974LysVal: 4.974 ± 0.479
1.135LysTrp: 1.135 ± 0.26
5.108LysTyr: 5.108 ± 0.38
0.0LysXaa: 0.0 ± 0.0
Leu
4.006LeuAla: 4.006 ± 0.49
1.168LeuCys: 1.168 ± 0.241
5.709LeuAsp: 5.709 ± 0.457
6.91LeuGlu: 6.91 ± 0.514
3.205LeuPhe: 3.205 ± 0.322
4.039LeuGly: 4.039 ± 0.346
0.968LeuHis: 0.968 ± 0.156
5.909LeuIle: 5.909 ± 0.403
8.179LeuLys: 8.179 ± 0.471
6.076LeuLeu: 6.076 ± 0.597
2.103LeuMet: 2.103 ± 0.266
6.476LeuAsn: 6.476 ± 0.469
2.237LeuPro: 2.237 ± 0.334
2.237LeuGln: 2.237 ± 0.348
3.739LeuArg: 3.739 ± 0.319
5.375LeuSer: 5.375 ± 0.383
3.872LeuThr: 3.872 ± 0.361
3.739LeuVal: 3.739 ± 0.334
0.568LeuTrp: 0.568 ± 0.144
3.872LeuTyr: 3.872 ± 0.328
0.0LeuXaa: 0.0 ± 0.0
Met
1.469MetAla: 1.469 ± 0.212
0.234MetCys: 0.234 ± 0.089
1.469MetAsp: 1.469 ± 0.231
2.137MetGlu: 2.137 ± 0.284
0.701MetPhe: 0.701 ± 0.179
1.102MetGly: 1.102 ± 0.214
0.601MetHis: 0.601 ± 0.141
2.036MetIle: 2.036 ± 0.266
2.27MetLys: 2.27 ± 0.322
1.469MetLeu: 1.469 ± 0.213
0.668MetMet: 0.668 ± 0.188
1.569MetAsn: 1.569 ± 0.236
0.568MetPro: 0.568 ± 0.148
0.801MetGln: 0.801 ± 0.193
1.102MetArg: 1.102 ± 0.177
1.636MetSer: 1.636 ± 0.268
1.235MetThr: 1.235 ± 0.172
1.269MetVal: 1.269 ± 0.189
0.134MetTrp: 0.134 ± 0.06
1.202MetTyr: 1.202 ± 0.235
0.0MetXaa: 0.0 ± 0.0
Asn
2.938AsnAla: 2.938 ± 0.341
0.634AsnCys: 0.634 ± 0.222
3.472AsnAsp: 3.472 ± 0.366
5.008AsnGlu: 5.008 ± 0.41
3.438AsnPhe: 3.438 ± 0.351
4.607AsnGly: 4.607 ± 0.662
1.469AsnHis: 1.469 ± 0.244
6.643AsnIle: 6.643 ± 0.52
7.044AsnLys: 7.044 ± 0.476
6.076AsnLeu: 6.076 ± 0.394
1.402AsnMet: 1.402 ± 0.213
6.143AsnAsn: 6.143 ± 0.584
2.337AsnPro: 2.337 ± 0.337
2.504AsnGln: 2.504 ± 0.255
2.27AsnArg: 2.27 ± 0.274
4.607AsnSer: 4.607 ± 0.477
3.572AsnThr: 3.572 ± 0.375
3.171AsnVal: 3.171 ± 0.349
0.768AsnTrp: 0.768 ± 0.171
3.973AsnTyr: 3.973 ± 0.4
0.0AsnXaa: 0.0 ± 0.0
Pro
1.302ProAla: 1.302 ± 0.275
0.367ProCys: 0.367 ± 0.13
1.836ProAsp: 1.836 ± 0.37
2.07ProGlu: 2.07 ± 0.287
1.703ProPhe: 1.703 ± 0.266
1.636ProGly: 1.636 ± 0.253
0.534ProHis: 0.534 ± 0.17
2.17ProIle: 2.17 ± 0.297
2.303ProLys: 2.303 ± 0.292
2.036ProLeu: 2.036 ± 0.292
0.734ProMet: 0.734 ± 0.164
1.736ProAsn: 1.736 ± 0.243
0.634ProPro: 0.634 ± 0.188
0.968ProGln: 0.968 ± 0.205
0.734ProArg: 0.734 ± 0.153
1.302ProSer: 1.302 ± 0.178
1.102ProThr: 1.102 ± 0.173
1.536ProVal: 1.536 ± 0.214
0.234ProTrp: 0.234 ± 0.097
1.369ProTyr: 1.369 ± 0.264
0.0ProXaa: 0.0 ± 0.0
Gln
1.703GlnAla: 1.703 ± 0.378
0.234GlnCys: 0.234 ± 0.085
1.703GlnAsp: 1.703 ± 0.283
2.938GlnGlu: 2.938 ± 0.41
1.302GlnPhe: 1.302 ± 0.254
1.469GlnGly: 1.469 ± 0.218
0.434GlnHis: 0.434 ± 0.123
2.571GlnIle: 2.571 ± 0.238
2.938GlnLys: 2.938 ± 0.362
2.47GlnLeu: 2.47 ± 0.256
0.634GlnMet: 0.634 ± 0.172
2.737GlnAsn: 2.737 ± 0.366
0.801GlnPro: 0.801 ± 0.168
1.502GlnGln: 1.502 ± 0.357
1.102GlnArg: 1.102 ± 0.174
1.803GlnSer: 1.803 ± 0.289
1.903GlnThr: 1.903 ± 0.285
1.469GlnVal: 1.469 ± 0.251
0.367GlnTrp: 0.367 ± 0.094
1.703GlnTyr: 1.703 ± 0.346
0.0GlnXaa: 0.0 ± 0.0
Arg
1.836ArgAla: 1.836 ± 0.285
0.3ArgCys: 0.3 ± 0.097
2.637ArgAsp: 2.637 ± 0.325
3.772ArgGlu: 3.772 ± 0.396
1.002ArgPhe: 1.002 ± 0.195
2.971ArgGly: 2.971 ± 0.326
0.467ArgHis: 0.467 ± 0.124
3.272ArgIle: 3.272 ± 0.365
3.806ArgLys: 3.806 ± 0.377
3.405ArgLeu: 3.405 ± 0.349
0.768ArgMet: 0.768 ± 0.163
2.103ArgAsn: 2.103 ± 0.286
1.002ArgPro: 1.002 ± 0.174
1.035ArgGln: 1.035 ± 0.212
1.636ArgArg: 1.636 ± 0.257
2.07ArgSer: 2.07 ± 0.219
1.869ArgThr: 1.869 ± 0.214
1.903ArgVal: 1.903 ± 0.23
0.401ArgTrp: 0.401 ± 0.111
1.803ArgTyr: 1.803 ± 0.24
0.0ArgXaa: 0.0 ± 0.0
Ser
2.771SerAla: 2.771 ± 0.403
0.668SerCys: 0.668 ± 0.166
3.906SerAsp: 3.906 ± 0.383
4.073SerGlu: 4.073 ± 0.335
2.871SerPhe: 2.871 ± 0.363
4.373SerGly: 4.373 ± 0.475
0.835SerHis: 0.835 ± 0.2
6.51SerIle: 6.51 ± 0.521
6.176SerLys: 6.176 ± 0.551
5.341SerLeu: 5.341 ± 0.454
1.235SerMet: 1.235 ± 0.22
4.407SerAsn: 4.407 ± 0.464
1.435SerPro: 1.435 ± 0.248
1.803SerGln: 1.803 ± 0.283
2.17SerArg: 2.17 ± 0.259
4.607SerSer: 4.607 ± 0.595
3.338SerThr: 3.338 ± 0.39
3.238SerVal: 3.238 ± 0.339
1.002SerTrp: 1.002 ± 0.198
3.238SerTyr: 3.238 ± 0.32
0.0SerXaa: 0.0 ± 0.0
Thr
2.938ThrAla: 2.938 ± 0.442
0.601ThrCys: 0.601 ± 0.149
3.071ThrAsp: 3.071 ± 0.356
3.605ThrGlu: 3.605 ± 0.416
2.203ThrPhe: 2.203 ± 0.251
3.071ThrGly: 3.071 ± 0.432
0.701ThrHis: 0.701 ± 0.169
4.44ThrIle: 4.44 ± 0.4
4.273ThrLys: 4.273 ± 0.404
4.44ThrLeu: 4.44 ± 0.416
1.002ThrMet: 1.002 ± 0.165
3.438ThrAsn: 3.438 ± 0.388
1.369ThrPro: 1.369 ± 0.24
1.869ThrGln: 1.869 ± 0.368
1.703ThrArg: 1.703 ± 0.265
3.706ThrSer: 3.706 ± 0.435
2.671ThrThr: 2.671 ± 0.322
2.804ThrVal: 2.804 ± 0.392
0.334ThrTrp: 0.334 ± 0.129
2.737ThrTyr: 2.737 ± 0.349
0.0ThrXaa: 0.0 ± 0.0
Val
2.036ValAla: 2.036 ± 0.323
0.835ValCys: 0.835 ± 0.17
4.14ValAsp: 4.14 ± 0.367
4.106ValGlu: 4.106 ± 0.479
1.936ValPhe: 1.936 ± 0.212
2.103ValGly: 2.103 ± 0.268
0.734ValHis: 0.734 ± 0.132
3.906ValIle: 3.906 ± 0.362
4.44ValLys: 4.44 ± 0.39
3.405ValLeu: 3.405 ± 0.318
1.168ValMet: 1.168 ± 0.158
3.438ValAsn: 3.438 ± 0.387
1.135ValPro: 1.135 ± 0.174
1.569ValGln: 1.569 ± 0.263
2.103ValArg: 2.103 ± 0.241
4.039ValSer: 4.039 ± 0.401
3.305ValThr: 3.305 ± 0.401
2.337ValVal: 2.337 ± 0.258
0.367ValTrp: 0.367 ± 0.112
2.604ValTyr: 2.604 ± 0.309
0.0ValXaa: 0.0 ± 0.0
Trp
0.501TrpAla: 0.501 ± 0.129
0.167TrpCys: 0.167 ± 0.073
0.668TrpAsp: 0.668 ± 0.148
0.768TrpGlu: 0.768 ± 0.177
0.401TrpPhe: 0.401 ± 0.129
0.568TrpGly: 0.568 ± 0.128
0.267TrpHis: 0.267 ± 0.101
0.968TrpIle: 0.968 ± 0.189
0.835TrpLys: 0.835 ± 0.179
1.002TrpLeu: 1.002 ± 0.207
0.134TrpMet: 0.134 ± 0.073
1.068TrpAsn: 1.068 ± 0.23
0.067TrpPro: 0.067 ± 0.044
0.167TrpGln: 0.167 ± 0.071
0.334TrpArg: 0.334 ± 0.098
0.734TrpSer: 0.734 ± 0.159
0.467TrpThr: 0.467 ± 0.14
0.534TrpVal: 0.534 ± 0.151
0.134TrpTrp: 0.134 ± 0.065
0.467TrpTyr: 0.467 ± 0.112
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.137TyrAla: 2.137 ± 0.268
1.035TyrCys: 1.035 ± 0.252
3.405TyrAsp: 3.405 ± 0.356
3.105TyrGlu: 3.105 ± 0.413
2.37TyrPhe: 2.37 ± 0.338
3.038TyrGly: 3.038 ± 0.293
0.835TyrHis: 0.835 ± 0.162
3.973TyrIle: 3.973 ± 0.427
3.973TyrLys: 3.973 ± 0.455
4.173TyrLeu: 4.173 ± 0.398
1.302TyrMet: 1.302 ± 0.245
4.206TyrAsn: 4.206 ± 0.428
1.803TyrPro: 1.803 ± 0.256
1.836TyrGln: 1.836 ± 0.219
2.17TyrArg: 2.17 ± 0.343
3.472TyrSer: 3.472 ± 0.461
2.404TyrThr: 2.404 ± 0.332
2.37TyrVal: 2.37 ± 0.256
0.601TyrTrp: 0.601 ± 0.159
2.804TyrTyr: 2.804 ± 0.345
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 115 proteins (29956 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski