Erigeron breviscapus amalgavirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; unclassified Amalgaviridae

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z6JIJ9|A0A2Z6JIJ9_9VIRU ORF1+2p OS=Erigeron breviscapus amalgavirus 1 OX=2069322 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.81GEE3 pKa = 4.42PLPEE7 pKa = 3.97LTYY10 pKa = 11.22VLTPAEE16 pKa = 4.18EE17 pKa = 4.13QAEE20 pKa = 4.62LTRR23 pKa = 11.84LAAPLIQQGLPAGLFDD39 pKa = 3.5RR40 pKa = 11.84TAVLRR45 pKa = 11.84ANYY48 pKa = 8.59TYY50 pKa = 11.04KK51 pKa = 11.0GFLKK55 pKa = 10.52HH56 pKa = 5.84LQTVSRR62 pKa = 11.84LVDD65 pKa = 3.03QDD67 pKa = 4.1IIVDD71 pKa = 3.86ALSLGVKK78 pKa = 9.79KK79 pKa = 10.74DD80 pKa = 3.7FFPLPNRR87 pKa = 11.84MNISQFCRR95 pKa = 11.84FSEE98 pKa = 4.01WLRR101 pKa = 11.84SKK103 pKa = 10.87DD104 pKa = 3.66GQNSLHH110 pKa = 5.9EE111 pKa = 4.34VQRR114 pKa = 11.84HH115 pKa = 4.54KK116 pKa = 11.05KK117 pKa = 10.09LEE119 pKa = 4.11KK120 pKa = 9.93KK121 pKa = 10.28AAGVLEE127 pKa = 4.1PRR129 pKa = 11.84EE130 pKa = 4.03VALEE134 pKa = 4.08QIFSAQRR141 pKa = 11.84ADD143 pKa = 2.74WAAAKK148 pKa = 10.36KK149 pKa = 9.07EE150 pKa = 3.98EE151 pKa = 3.85RR152 pKa = 11.84SAYY155 pKa = 10.16DD156 pKa = 3.8KK157 pKa = 10.77EE158 pKa = 4.16IQEE161 pKa = 4.1LRR163 pKa = 11.84KK164 pKa = 10.02KK165 pKa = 10.05IRR167 pKa = 11.84QLEE170 pKa = 4.28RR171 pKa = 11.84RR172 pKa = 11.84WEE174 pKa = 3.97RR175 pKa = 11.84RR176 pKa = 11.84EE177 pKa = 3.88VEE179 pKa = 3.71IDD181 pKa = 3.28NAFDD185 pKa = 3.58PQFEE189 pKa = 4.09FLEE192 pKa = 4.37LDD194 pKa = 3.36EE195 pKa = 5.24AALNEE200 pKa = 4.23RR201 pKa = 11.84AYY203 pKa = 11.06AMYY206 pKa = 11.01VHH208 pKa = 7.48DD209 pKa = 4.52CQVKK213 pKa = 8.77EE214 pKa = 3.68RR215 pKa = 11.84RR216 pKa = 11.84PRR218 pKa = 11.84SKK220 pKa = 10.58EE221 pKa = 3.51HH222 pKa = 6.67GGVQLAVEE230 pKa = 4.28AFGGQVKK237 pKa = 9.76KK238 pKa = 10.73QKK240 pKa = 9.2QAEE243 pKa = 4.33FARR246 pKa = 11.84EE247 pKa = 3.69PEE249 pKa = 4.25VAVKK253 pKa = 10.48LFEE256 pKa = 3.97YY257 pKa = 10.35AKK259 pKa = 10.47RR260 pKa = 11.84KK261 pKa = 9.19ILSFEE266 pKa = 4.05EE267 pKa = 4.43MVTSNRR273 pKa = 11.84KK274 pKa = 9.08PIGKK278 pKa = 7.4FTWMPRR284 pKa = 11.84VEE286 pKa = 4.49TYY288 pKa = 10.97LLSRR292 pKa = 11.84PVKK295 pKa = 9.5LRR297 pKa = 11.84KK298 pKa = 9.49RR299 pKa = 11.84ICSMCPVGLPPPPGVAPGCQPLSKK323 pKa = 10.25FVRR326 pKa = 11.84EE327 pKa = 4.0EE328 pKa = 3.94LLVSGVVEE336 pKa = 4.49GTKK339 pKa = 8.78KK340 pKa = 9.35TNPLLRR346 pKa = 11.84AFATVQLGAEE356 pKa = 4.53VLRR359 pKa = 11.84STRR362 pKa = 11.84VLVHH366 pKa = 5.67HH367 pKa = 6.55TFGPEE372 pKa = 3.59TQRR375 pKa = 11.84RR376 pKa = 11.84IPVARR381 pKa = 11.84SRR383 pKa = 11.84IEE385 pKa = 3.66AGLRR389 pKa = 11.84RR390 pKa = 11.84IIGGGAMRR398 pKa = 11.84GWDD401 pKa = 3.79ADD403 pKa = 3.31SKK405 pKa = 9.87MFRR408 pKa = 11.84GGGNSSDD415 pKa = 3.7ALLLLGQCDD424 pKa = 3.94DD425 pKa = 4.34NLPGGLLRR433 pKa = 11.84EE434 pKa = 4.32HH435 pKa = 7.1FSLLSAKK442 pKa = 10.02RR443 pKa = 11.84ALSLPGGLRR452 pKa = 11.84VPDD455 pKa = 4.22GPEE458 pKa = 3.76CLVMKK463 pKa = 10.39NFNNDD468 pKa = 2.34ATAGPFLRR476 pKa = 11.84AFGIKK481 pKa = 9.77GKK483 pKa = 10.85YY484 pKa = 8.38GLKK487 pKa = 10.36KK488 pKa = 10.53LLEE491 pKa = 4.23DD492 pKa = 3.92TMWWFYY498 pKa = 11.32DD499 pKa = 3.19AYY501 pKa = 10.53GRR503 pKa = 11.84GEE505 pKa = 3.96ISDD508 pKa = 4.17GEE510 pKa = 4.38MPHH513 pKa = 6.08FAARR517 pKa = 11.84VGFRR521 pKa = 11.84TKK523 pKa = 10.53LVSEE527 pKa = 4.57TKK529 pKa = 10.29AWEE532 pKa = 4.04KK533 pKa = 10.44LAAGAPVGRR542 pKa = 11.84AVMMLDD548 pKa = 3.68ALEE551 pKa = 4.23QAASSPLYY559 pKa = 10.78NVMSNSTYY567 pKa = 8.49QRR569 pKa = 11.84RR570 pKa = 11.84LEE572 pKa = 4.14RR573 pKa = 11.84DD574 pKa = 3.29CGFKK578 pKa = 10.82NGIVKK583 pKa = 10.64ASSDD587 pKa = 3.09WAKK590 pKa = 9.4IWEE593 pKa = 4.35DD594 pKa = 3.26VRR596 pKa = 11.84EE597 pKa = 4.15AKK599 pKa = 10.51AIIEE603 pKa = 4.2LDD605 pKa = 2.79WSKK608 pKa = 10.98FDD610 pKa = 4.39RR611 pKa = 11.84EE612 pKa = 4.13RR613 pKa = 11.84PADD616 pKa = 4.63DD617 pKa = 3.13ILFVIEE623 pKa = 4.31VVLSCFEE630 pKa = 3.96PTNDD634 pKa = 3.58RR635 pKa = 11.84EE636 pKa = 4.18RR637 pKa = 11.84RR638 pKa = 11.84LLRR641 pKa = 11.84AFGLMMRR648 pKa = 11.84RR649 pKa = 11.84ALVEE653 pKa = 4.31RR654 pKa = 11.84IIVMDD659 pKa = 4.28DD660 pKa = 3.26GGVFEE665 pKa = 5.25IDD667 pKa = 3.19GMVPSGSLWTGWLDD681 pKa = 3.27TALNVLYY688 pKa = 11.01LNAACMNVGIGPLGFSAMCAGDD710 pKa = 4.73DD711 pKa = 3.97NLTLFWTDD719 pKa = 3.5HH720 pKa = 6.94PDD722 pKa = 3.38HH723 pKa = 6.08VLKK726 pKa = 10.74RR727 pKa = 11.84IKK729 pKa = 10.69DD730 pKa = 3.6EE731 pKa = 4.35LNGKK735 pKa = 8.76FRR737 pKa = 11.84AGISDD742 pKa = 3.56EE743 pKa = 4.88DD744 pKa = 4.33FFIHH748 pKa = 7.07RR749 pKa = 11.84PPFHH753 pKa = 5.87VTKK756 pKa = 10.44QQACFPPGTDD766 pKa = 3.76LSHH769 pKa = 6.04GTSKK773 pKa = 11.07LMDD776 pKa = 3.6LVFWQVFDD784 pKa = 4.12GEE786 pKa = 4.69VVIDD790 pKa = 3.88EE791 pKa = 4.36AAGRR795 pKa = 11.84SHH797 pKa = 6.4RR798 pKa = 11.84WEE800 pKa = 3.97YY801 pKa = 10.42VFKK804 pKa = 10.79GRR806 pKa = 11.84PKK808 pKa = 10.44FLSCYY813 pKa = 8.04WLPGGQPIRR822 pKa = 11.84PTSDD826 pKa = 2.72NMEE829 pKa = 4.22KK830 pKa = 10.73LLWPEE835 pKa = 5.26GIHH838 pKa = 7.04EE839 pKa = 6.15DD840 pKa = 3.61IDD842 pKa = 4.85DD843 pKa = 4.04YY844 pKa = 11.5QATVMAMVVDD854 pKa = 4.36NPWNHH859 pKa = 6.23HH860 pKa = 5.61CVNHH864 pKa = 6.19LLMRR868 pKa = 11.84YY869 pKa = 9.63VILQQLRR876 pKa = 11.84RR877 pKa = 11.84VDD879 pKa = 3.43ILRR882 pKa = 11.84GGMDD886 pKa = 4.48DD887 pKa = 4.85VLFLCACRR895 pKa = 11.84EE896 pKa = 4.13KK897 pKa = 11.14GGGPIPYY904 pKa = 9.81PMVAPWRR911 pKa = 11.84RR912 pKa = 11.84SEE914 pKa = 3.69VHH916 pKa = 6.38GRR918 pKa = 11.84MEE920 pKa = 4.99DD921 pKa = 3.56YY922 pKa = 11.33EE923 pKa = 4.37EE924 pKa = 4.28VKK926 pKa = 10.71RR927 pKa = 11.84HH928 pKa = 5.44IQDD931 pKa = 3.08FSDD934 pKa = 3.71FVTGVTSLYY943 pKa = 10.89SRR945 pKa = 11.84TATGGVDD952 pKa = 2.57SWLFMNIIRR961 pKa = 11.84GEE963 pKa = 3.96QHH965 pKa = 6.07VGEE968 pKa = 4.3GQYY971 pKa = 11.66GNDD974 pKa = 3.07LMVWVSWIRR983 pKa = 11.84DD984 pKa = 3.89HH985 pKa = 7.6PCTRR989 pKa = 11.84YY990 pKa = 9.67LKK992 pKa = 10.02SVRR995 pKa = 11.84GLRR998 pKa = 11.84TRR1000 pKa = 11.84VEE1002 pKa = 4.07QLDD1005 pKa = 3.79VDD1007 pKa = 4.1PGLLQKK1013 pKa = 10.2ATIHH1017 pKa = 5.94YY1018 pKa = 9.58SLLRR1022 pKa = 11.84EE1023 pKa = 4.07TLVSGRR1029 pKa = 11.84IEE1031 pKa = 3.99TALDD1035 pKa = 3.46FARR1038 pKa = 11.84WVRR1041 pKa = 11.84SIILGGHH1048 pKa = 4.98VV1049 pKa = 3.17

Molecular weight: 119.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z6JIJ9|A0A2Z6JIJ9_9VIRU ORF1+2p OS=Erigeron breviscapus amalgavirus 1 OX=2069322 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.81GEE3 pKa = 4.42PLPEE7 pKa = 3.97LTYY10 pKa = 11.22VLTPAEE16 pKa = 4.18EE17 pKa = 4.13QAEE20 pKa = 4.62LTRR23 pKa = 11.84LAAPLIQQGLPAGLFDD39 pKa = 3.5RR40 pKa = 11.84TAVLRR45 pKa = 11.84ANYY48 pKa = 8.59TYY50 pKa = 11.04KK51 pKa = 11.0GFLKK55 pKa = 10.52HH56 pKa = 5.84LQTVSRR62 pKa = 11.84LVDD65 pKa = 3.03QDD67 pKa = 4.1IIVDD71 pKa = 3.86ALSLGVKK78 pKa = 9.79KK79 pKa = 10.74DD80 pKa = 3.7FFPLPNRR87 pKa = 11.84MNISQFCRR95 pKa = 11.84FSEE98 pKa = 4.01WLRR101 pKa = 11.84SKK103 pKa = 10.87DD104 pKa = 3.66GQNSLHH110 pKa = 5.9EE111 pKa = 4.34VQRR114 pKa = 11.84HH115 pKa = 4.54KK116 pKa = 11.05KK117 pKa = 10.09LEE119 pKa = 4.11KK120 pKa = 9.93KK121 pKa = 10.28AAGVLEE127 pKa = 4.1PRR129 pKa = 11.84EE130 pKa = 4.03VALEE134 pKa = 4.08QIFSAQRR141 pKa = 11.84ADD143 pKa = 2.74WAAAKK148 pKa = 10.36KK149 pKa = 9.07EE150 pKa = 3.98EE151 pKa = 3.85RR152 pKa = 11.84SAYY155 pKa = 10.16DD156 pKa = 3.8KK157 pKa = 10.77EE158 pKa = 4.16IQEE161 pKa = 4.1LRR163 pKa = 11.84KK164 pKa = 10.02KK165 pKa = 10.05IRR167 pKa = 11.84QLEE170 pKa = 4.28RR171 pKa = 11.84RR172 pKa = 11.84WEE174 pKa = 3.97RR175 pKa = 11.84RR176 pKa = 11.84EE177 pKa = 3.88VEE179 pKa = 3.71IDD181 pKa = 3.28NAFDD185 pKa = 3.58PQFEE189 pKa = 4.09FLEE192 pKa = 4.37LDD194 pKa = 3.36EE195 pKa = 5.24AALNEE200 pKa = 4.23RR201 pKa = 11.84AYY203 pKa = 11.06AMYY206 pKa = 11.01VHH208 pKa = 7.48DD209 pKa = 4.52CQVKK213 pKa = 8.77EE214 pKa = 3.68RR215 pKa = 11.84RR216 pKa = 11.84PRR218 pKa = 11.84SKK220 pKa = 10.58EE221 pKa = 3.51HH222 pKa = 6.67GGVQLAVEE230 pKa = 4.28AFGGQVKK237 pKa = 9.76KK238 pKa = 10.73QKK240 pKa = 9.2QAEE243 pKa = 4.33FARR246 pKa = 11.84EE247 pKa = 3.69PEE249 pKa = 4.25VAVKK253 pKa = 10.48LFEE256 pKa = 3.97YY257 pKa = 10.35AKK259 pKa = 10.52RR260 pKa = 11.84KK261 pKa = 9.19ILSFRR266 pKa = 11.84RR267 pKa = 11.84DD268 pKa = 2.84GDD270 pKa = 3.5IKK272 pKa = 11.08QEE274 pKa = 4.31ANWKK278 pKa = 10.16VYY280 pKa = 9.84MDD282 pKa = 4.07AAGGDD287 pKa = 4.22LSTVPTGEE295 pKa = 3.99APEE298 pKa = 4.34EE299 pKa = 3.86NLLYY303 pKa = 10.45VPRR306 pKa = 11.84GVTPPPRR313 pKa = 11.84GGPGVPTAQQVRR325 pKa = 11.84QGGVTGVRR333 pKa = 11.84RR334 pKa = 11.84GRR336 pKa = 11.84GDD338 pKa = 4.61EE339 pKa = 3.97EE340 pKa = 4.29DD341 pKa = 3.52QPIAARR347 pKa = 11.84LRR349 pKa = 11.84NRR351 pKa = 11.84PTRR354 pKa = 11.84SRR356 pKa = 11.84SIEE359 pKa = 3.77EE360 pKa = 3.93HH361 pKa = 5.85QGVGTSHH368 pKa = 6.61IRR370 pKa = 11.84AGDD373 pKa = 3.58TTEE376 pKa = 4.35DD377 pKa = 3.42TGSSLPHH384 pKa = 6.23

Molecular weight: 43.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1433

384

1049

716.5

81.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.886 ± 0.775

1.326 ± 0.419

5.792 ± 0.304

7.955 ± 1.145

4.327 ± 0.354

7.746 ± 0.236

2.303 ± 0.114

4.047 ± 0.344

5.583 ± 0.347

9.839 ± 0.648

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.373 ± 0.692

2.582 ± 0.124

5.234 ± 0.258

4.187 ± 1.073

8.723 ± 0.475

4.745 ± 0.301

3.838 ± 0.171

7.258 ± 0.253

1.954 ± 0.475

2.303 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski