Gordonia phage Secretariat

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Deejayvirinae; Secretariatvirus; Gordonia virus Secretariat

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3T6Q2|A0A6M3T6Q2_9CAUD Uncharacterized protein OS=Gordonia phage Secretariat OX=2725616 GN=34 PE=4 SV=1
MM1 pKa = 7.61TEE3 pKa = 4.44IKK5 pKa = 9.84WDD7 pKa = 3.58TAGDD11 pKa = 3.55RR12 pKa = 11.84LYY14 pKa = 10.49EE15 pKa = 4.08TGVDD19 pKa = 3.47HH20 pKa = 6.95GVLYY24 pKa = 10.57IPDD27 pKa = 3.55NTGAYY32 pKa = 9.44AVGYY36 pKa = 8.98AWNGLTSVSEE46 pKa = 4.4SPSGAEE52 pKa = 4.95ANPQYY57 pKa = 11.34ADD59 pKa = 3.47NIKK62 pKa = 10.1YY63 pKa = 10.68LNLISAEE70 pKa = 4.05EE71 pKa = 3.95FGATIEE77 pKa = 4.34AFTYY81 pKa = 10.26PDD83 pKa = 4.6AFAQCDD89 pKa = 3.53GTAIIGGVQIAQQTRR104 pKa = 11.84KK105 pKa = 10.29SFGFSYY111 pKa = 9.38RR112 pKa = 11.84TLIGNDD118 pKa = 2.75IVGTDD123 pKa = 3.73FGYY126 pKa = 11.01KK127 pKa = 9.5LHH129 pKa = 6.76LVYY132 pKa = 10.84GCDD135 pKa = 3.3AAPSEE140 pKa = 4.53KK141 pKa = 10.32SRR143 pKa = 11.84STVNDD148 pKa = 3.36SPEE151 pKa = 3.65AATFSWEE158 pKa = 3.66ITTNPVPVAGINAATGKK175 pKa = 9.61PYY177 pKa = 10.69RR178 pKa = 11.84PTAHH182 pKa = 5.39VTIDD186 pKa = 3.52STKK189 pKa = 10.53VNPADD194 pKa = 3.56MAALEE199 pKa = 4.68AILYY203 pKa = 9.86GSADD207 pKa = 3.8TEE209 pKa = 4.13PRR211 pKa = 11.84MPMPEE216 pKa = 3.99EE217 pKa = 3.9VLSLVGEE224 pKa = 4.25AATLVTATPPSFDD237 pKa = 4.24APSDD241 pKa = 3.77TITIPTVTGVVYY253 pKa = 9.87MIDD256 pKa = 3.64GVPVTGEE263 pKa = 3.97EE264 pKa = 4.11IIEE267 pKa = 4.25EE268 pKa = 4.43VTTVTAQADD277 pKa = 3.37VGYY280 pKa = 9.35RR281 pKa = 11.84LPAGAVTSWTFTPP294 pKa = 4.44

Molecular weight: 31.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3SUM2|A0A6M3SUM2_9CAUD Uncharacterized protein OS=Gordonia phage Secretariat OX=2725616 GN=59 PE=4 SV=1
MM1 pKa = 7.46IWFDD5 pKa = 3.01ILTYY9 pKa = 7.58TICVLFVWAVYY20 pKa = 10.24DD21 pKa = 3.69GSKK24 pKa = 10.26RR25 pKa = 11.84IVRR28 pKa = 11.84AIRR31 pKa = 11.84NRR33 pKa = 11.84KK34 pKa = 6.92VRR36 pKa = 11.84KK37 pKa = 9.57HH38 pKa = 5.56EE39 pKa = 4.56SGSS42 pKa = 3.48

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

18052

30

1697

214.9

23.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.47 ± 0.469

0.604 ± 0.099

6.193 ± 0.247

7.085 ± 0.328

3.501 ± 0.237

7.667 ± 0.476

1.895 ± 0.168

5.916 ± 0.271

5.983 ± 0.365

7.85 ± 0.365

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.858 ± 0.165

4.404 ± 0.184

4.005 ± 0.24

3.379 ± 0.127

5.102 ± 0.343

6.326 ± 0.197

6.686 ± 0.265

7.312 ± 0.26

1.352 ± 0.111

3.412 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski