Amino acid dipepetide frequency for Gordonia phage Secretariat

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.753AlaAla: 8.753 ± 1.299
0.332AlaCys: 0.332 ± 0.122
4.986AlaAsp: 4.986 ± 0.559
4.986AlaGlu: 4.986 ± 0.471
3.269AlaPhe: 3.269 ± 0.561
6.038AlaGly: 6.038 ± 0.935
1.717AlaHis: 1.717 ± 0.35
6.094AlaIle: 6.094 ± 0.742
5.54AlaLys: 5.54 ± 0.537
6.371AlaLeu: 6.371 ± 1.062
3.324AlaMet: 3.324 ± 0.597
3.823AlaAsn: 3.823 ± 0.471
2.271AlaPro: 2.271 ± 0.303
3.102AlaGln: 3.102 ± 0.38
4.21AlaArg: 4.21 ± 0.558
5.097AlaSer: 5.097 ± 0.539
5.097AlaThr: 5.097 ± 0.645
5.872AlaVal: 5.872 ± 0.602
1.053AlaTrp: 1.053 ± 0.199
2.77AlaTyr: 2.77 ± 0.385
0.0AlaXaa: 0.0 ± 0.0
Cys
0.443CysAla: 0.443 ± 0.14
0.055CysCys: 0.055 ± 0.054
0.499CysAsp: 0.499 ± 0.184
0.222CysGlu: 0.222 ± 0.108
0.222CysPhe: 0.222 ± 0.115
0.332CysGly: 0.332 ± 0.141
0.166CysHis: 0.166 ± 0.094
0.443CysIle: 0.443 ± 0.161
0.277CysLys: 0.277 ± 0.15
0.665CysLeu: 0.665 ± 0.226
0.111CysMet: 0.111 ± 0.071
0.222CysAsn: 0.222 ± 0.125
0.222CysPro: 0.222 ± 0.13
0.055CysGln: 0.055 ± 0.055
0.499CysArg: 0.499 ± 0.219
0.388CysSer: 0.388 ± 0.126
0.388CysThr: 0.388 ± 0.172
0.609CysVal: 0.609 ± 0.206
0.111CysTrp: 0.111 ± 0.066
0.111CysTyr: 0.111 ± 0.077
0.0CysXaa: 0.0 ± 0.0
Asp
5.761AspAla: 5.761 ± 0.62
0.332AspCys: 0.332 ± 0.124
3.767AspAsp: 3.767 ± 0.937
5.484AspGlu: 5.484 ± 0.65
3.102AspPhe: 3.102 ± 0.326
5.097AspGly: 5.097 ± 0.464
1.163AspHis: 1.163 ± 0.27
2.715AspIle: 2.715 ± 0.377
2.659AspLys: 2.659 ± 0.366
5.484AspLeu: 5.484 ± 0.695
2.05AspMet: 2.05 ± 0.354
2.659AspAsn: 2.659 ± 0.412
2.493AspPro: 2.493 ± 0.382
2.216AspGln: 2.216 ± 0.429
3.102AspArg: 3.102 ± 0.422
3.656AspSer: 3.656 ± 0.725
3.379AspThr: 3.379 ± 0.345
4.432AspVal: 4.432 ± 0.397
0.665AspTrp: 0.665 ± 0.156
1.662AspTyr: 1.662 ± 0.321
0.0AspXaa: 0.0 ± 0.0
Glu
6.205GluAla: 6.205 ± 0.523
0.499GluCys: 0.499 ± 0.189
4.321GluAsp: 4.321 ± 0.473
6.094GluGlu: 6.094 ± 0.806
2.216GluPhe: 2.216 ± 0.391
5.152GluGly: 5.152 ± 0.41
0.997GluHis: 0.997 ± 0.232
4.099GluIle: 4.099 ± 0.484
5.097GluLys: 5.097 ± 0.684
6.038GluLeu: 6.038 ± 0.535
2.438GluMet: 2.438 ± 0.357
2.881GluAsn: 2.881 ± 0.45
2.05GluPro: 2.05 ± 0.428
3.158GluGln: 3.158 ± 0.471
3.047GluArg: 3.047 ± 0.54
3.102GluSer: 3.102 ± 0.429
4.598GluThr: 4.598 ± 0.497
4.764GluVal: 4.764 ± 0.421
1.551GluTrp: 1.551 ± 0.324
2.548GluTyr: 2.548 ± 0.409
0.0GluXaa: 0.0 ± 0.0
Phe
2.438PheAla: 2.438 ± 0.39
0.332PheCys: 0.332 ± 0.133
3.656PheAsp: 3.656 ± 0.415
3.102PheGlu: 3.102 ± 0.403
0.997PhePhe: 0.997 ± 0.251
3.102PheGly: 3.102 ± 0.524
0.72PheHis: 0.72 ± 0.173
1.773PheIle: 1.773 ± 0.339
1.662PheLys: 1.662 ± 0.381
2.548PheLeu: 2.548 ± 0.297
0.997PheMet: 0.997 ± 0.231
1.994PheAsn: 1.994 ± 0.314
1.33PhePro: 1.33 ± 0.264
0.831PheGln: 0.831 ± 0.222
1.939PheArg: 1.939 ± 0.312
1.939PheSer: 1.939 ± 0.339
1.939PheThr: 1.939 ± 0.29
2.161PheVal: 2.161 ± 0.328
0.332PheTrp: 0.332 ± 0.097
1.219PheTyr: 1.219 ± 0.292
0.0PheXaa: 0.0 ± 0.0
Gly
6.149GlyAla: 6.149 ± 0.973
0.499GlyCys: 0.499 ± 0.182
5.207GlyAsp: 5.207 ± 0.41
4.82GlyGlu: 4.82 ± 0.352
2.881GlyPhe: 2.881 ± 0.456
6.482GlyGly: 6.482 ± 0.911
1.163GlyHis: 1.163 ± 0.27
5.54GlyIle: 5.54 ± 1.006
3.601GlyLys: 3.601 ± 0.423
5.872GlyLeu: 5.872 ± 1.339
2.881GlyMet: 2.881 ± 0.552
3.213GlyAsn: 3.213 ± 0.472
2.382GlyPro: 2.382 ± 0.295
1.884GlyGln: 1.884 ± 0.269
2.992GlyArg: 2.992 ± 0.414
5.263GlySer: 5.263 ± 0.682
5.872GlyThr: 5.872 ± 0.59
6.038GlyVal: 6.038 ± 0.677
1.053GlyTrp: 1.053 ± 0.25
2.881GlyTyr: 2.881 ± 0.449
0.0GlyXaa: 0.0 ± 0.0
His
1.385HisAla: 1.385 ± 0.301
0.222HisCys: 0.222 ± 0.115
1.053HisAsp: 1.053 ± 0.236
1.219HisGlu: 1.219 ± 0.344
0.665HisPhe: 0.665 ± 0.169
1.717HisGly: 1.717 ± 0.354
0.609HisHis: 0.609 ± 0.163
1.607HisIle: 1.607 ± 0.32
0.609HisLys: 0.609 ± 0.18
1.385HisLeu: 1.385 ± 0.232
0.332HisMet: 0.332 ± 0.123
0.554HisAsn: 0.554 ± 0.197
0.776HisPro: 0.776 ± 0.233
0.388HisGln: 0.388 ± 0.141
1.108HisArg: 1.108 ± 0.23
1.385HisSer: 1.385 ± 0.266
1.053HisThr: 1.053 ± 0.225
1.717HisVal: 1.717 ± 0.351
0.111HisTrp: 0.111 ± 0.071
1.053HisTyr: 1.053 ± 0.247
0.0HisXaa: 0.0 ± 0.0
Ile
5.152IleAla: 5.152 ± 0.787
0.499IleCys: 0.499 ± 0.164
4.21IleAsp: 4.21 ± 0.368
4.709IleGlu: 4.709 ± 0.585
2.438IlePhe: 2.438 ± 0.36
4.598IleGly: 4.598 ± 0.784
1.053IleHis: 1.053 ± 0.23
3.047IleIle: 3.047 ± 0.581
3.269IleLys: 3.269 ± 0.436
4.764IleLeu: 4.764 ± 0.479
0.997IleMet: 0.997 ± 0.309
2.05IleAsn: 2.05 ± 0.289
2.327IlePro: 2.327 ± 0.292
2.77IleGln: 2.77 ± 0.349
2.659IleArg: 2.659 ± 0.417
2.715IleSer: 2.715 ± 0.54
3.989IleThr: 3.989 ± 0.409
5.263IleVal: 5.263 ± 0.567
1.053IleTrp: 1.053 ± 0.212
1.607IleTyr: 1.607 ± 0.278
0.0IleXaa: 0.0 ± 0.0
Lys
5.041LysAla: 5.041 ± 0.461
0.166LysCys: 0.166 ± 0.088
3.49LysAsp: 3.49 ± 0.57
4.21LysGlu: 4.21 ± 0.484
2.05LysPhe: 2.05 ± 0.455
4.155LysGly: 4.155 ± 0.629
1.33LysHis: 1.33 ± 0.293
3.823LysIle: 3.823 ± 0.495
3.601LysLys: 3.601 ± 0.592
4.598LysLeu: 4.598 ± 0.527
1.717LysMet: 1.717 ± 0.296
2.548LysAsn: 2.548 ± 0.39
2.438LysPro: 2.438 ± 0.501
2.382LysGln: 2.382 ± 0.32
2.659LysArg: 2.659 ± 0.386
3.213LysSer: 3.213 ± 0.327
3.823LysThr: 3.823 ± 0.478
5.207LysVal: 5.207 ± 0.584
1.108LysTrp: 1.108 ± 0.276
2.271LysTyr: 2.271 ± 0.36
0.0LysXaa: 0.0 ± 0.0
Leu
6.315LeuAla: 6.315 ± 0.923
0.443LeuCys: 0.443 ± 0.145
5.595LeuAsp: 5.595 ± 0.487
4.321LeuGlu: 4.321 ± 0.563
2.659LeuPhe: 2.659 ± 0.38
6.094LeuGly: 6.094 ± 1.153
1.219LeuHis: 1.219 ± 0.301
4.266LeuIle: 4.266 ± 0.513
5.041LeuLys: 5.041 ± 0.813
4.82LeuLeu: 4.82 ± 0.581
1.994LeuMet: 1.994 ± 0.296
4.82LeuAsn: 4.82 ± 0.569
2.715LeuPro: 2.715 ± 0.364
2.438LeuGln: 2.438 ± 0.315
3.989LeuArg: 3.989 ± 0.44
6.315LeuSer: 6.315 ± 0.564
6.426LeuThr: 6.426 ± 0.479
5.54LeuVal: 5.54 ± 0.736
0.831LeuTrp: 0.831 ± 0.188
2.659LeuTyr: 2.659 ± 0.502
0.0LeuXaa: 0.0 ± 0.0
Met
2.327MetAla: 2.327 ± 0.395
0.055MetCys: 0.055 ± 0.057
1.219MetAsp: 1.219 ± 0.23
1.662MetGlu: 1.662 ± 0.298
0.72MetPhe: 0.72 ± 0.156
2.327MetGly: 2.327 ± 0.532
0.609MetHis: 0.609 ± 0.164
1.662MetIle: 1.662 ± 0.282
2.327MetLys: 2.327 ± 0.376
2.604MetLeu: 2.604 ± 0.339
0.776MetMet: 0.776 ± 0.218
1.385MetAsn: 1.385 ± 0.304
0.997MetPro: 0.997 ± 0.217
0.554MetGln: 0.554 ± 0.177
1.33MetArg: 1.33 ± 0.342
2.659MetSer: 2.659 ± 0.346
2.77MetThr: 2.77 ± 0.3
1.994MetVal: 1.994 ± 0.328
0.166MetTrp: 0.166 ± 0.101
0.443MetTyr: 0.443 ± 0.137
0.0MetXaa: 0.0 ± 0.0
Asn
3.269AsnAla: 3.269 ± 0.34
0.443AsnCys: 0.443 ± 0.165
2.493AsnAsp: 2.493 ± 0.475
3.601AsnGlu: 3.601 ± 0.406
1.385AsnPhe: 1.385 ± 0.314
3.546AsnGly: 3.546 ± 0.533
0.997AsnHis: 0.997 ± 0.239
1.939AsnIle: 1.939 ± 0.223
2.881AsnLys: 2.881 ± 0.48
3.158AsnLeu: 3.158 ± 0.474
1.274AsnMet: 1.274 ± 0.247
1.773AsnAsn: 1.773 ± 0.37
3.102AsnPro: 3.102 ± 0.499
1.717AsnGln: 1.717 ± 0.298
2.77AsnArg: 2.77 ± 0.433
2.493AsnSer: 2.493 ± 0.388
2.271AsnThr: 2.271 ± 0.281
3.213AsnVal: 3.213 ± 0.4
0.277AsnTrp: 0.277 ± 0.104
1.44AsnTyr: 1.44 ± 0.246
0.0AsnXaa: 0.0 ± 0.0
Pro
2.992ProAla: 2.992 ± 0.321
0.332ProCys: 0.332 ± 0.138
2.659ProAsp: 2.659 ± 0.487
3.213ProGlu: 3.213 ± 0.36
1.551ProPhe: 1.551 ± 0.277
2.992ProGly: 2.992 ± 0.483
0.997ProHis: 0.997 ± 0.293
1.773ProIle: 1.773 ± 0.316
2.659ProLys: 2.659 ± 0.404
2.271ProLeu: 2.271 ± 0.319
1.108ProMet: 1.108 ± 0.28
1.884ProAsn: 1.884 ± 0.302
2.271ProPro: 2.271 ± 0.481
1.496ProGln: 1.496 ± 0.225
1.662ProArg: 1.662 ± 0.244
2.77ProSer: 2.77 ± 0.465
2.216ProThr: 2.216 ± 0.45
3.269ProVal: 3.269 ± 0.39
0.443ProTrp: 0.443 ± 0.132
1.496ProTyr: 1.496 ± 0.275
0.0ProXaa: 0.0 ± 0.0
Gln
3.269GlnAla: 3.269 ± 0.425
0.055GlnCys: 0.055 ± 0.057
1.607GlnAsp: 1.607 ± 0.244
2.271GlnGlu: 2.271 ± 0.353
1.219GlnPhe: 1.219 ± 0.29
2.105GlnGly: 2.105 ± 0.306
0.886GlnHis: 0.886 ± 0.277
1.884GlnIle: 1.884 ± 0.245
2.105GlnLys: 2.105 ± 0.339
3.379GlnLeu: 3.379 ± 0.382
1.053GlnMet: 1.053 ± 0.295
1.385GlnAsn: 1.385 ± 0.274
1.385GlnPro: 1.385 ± 0.294
1.108GlnGln: 1.108 ± 0.208
1.773GlnArg: 1.773 ± 0.284
2.382GlnSer: 2.382 ± 0.357
2.327GlnThr: 2.327 ± 0.323
2.161GlnVal: 2.161 ± 0.336
0.388GlnTrp: 0.388 ± 0.157
1.053GlnTyr: 1.053 ± 0.245
0.0GlnXaa: 0.0 ± 0.0
Arg
3.379ArgAla: 3.379 ± 0.482
0.222ArgCys: 0.222 ± 0.107
3.047ArgAsp: 3.047 ± 0.372
3.49ArgGlu: 3.49 ± 0.551
1.939ArgPhe: 1.939 ± 0.31
3.213ArgGly: 3.213 ± 0.499
1.219ArgHis: 1.219 ± 0.296
3.269ArgIle: 3.269 ± 0.335
3.601ArgLys: 3.601 ± 0.569
4.487ArgLeu: 4.487 ± 0.486
1.163ArgMet: 1.163 ± 0.25
2.715ArgAsn: 2.715 ± 0.379
2.825ArgPro: 2.825 ± 0.421
1.828ArgGln: 1.828 ± 0.378
3.49ArgArg: 3.49 ± 0.542
2.77ArgSer: 2.77 ± 0.384
3.102ArgThr: 3.102 ± 0.38
2.77ArgVal: 2.77 ± 0.455
0.831ArgTrp: 0.831 ± 0.246
1.662ArgTyr: 1.662 ± 0.29
0.0ArgXaa: 0.0 ± 0.0
Ser
3.989SerAla: 3.989 ± 0.537
0.111SerCys: 0.111 ± 0.084
3.823SerAsp: 3.823 ± 0.607
4.543SerGlu: 4.543 ± 0.524
2.105SerPhe: 2.105 ± 0.341
5.817SerGly: 5.817 ± 0.601
1.053SerHis: 1.053 ± 0.224
3.324SerIle: 3.324 ± 0.298
3.823SerLys: 3.823 ± 0.453
5.318SerLeu: 5.318 ± 0.556
2.05SerMet: 2.05 ± 0.437
3.102SerAsn: 3.102 ± 0.406
2.105SerPro: 2.105 ± 0.296
2.327SerGln: 2.327 ± 0.426
2.992SerArg: 2.992 ± 0.352
4.044SerSer: 4.044 ± 0.49
4.709SerThr: 4.709 ± 0.545
4.099SerVal: 4.099 ± 0.447
1.385SerTrp: 1.385 ± 0.265
2.548SerTyr: 2.548 ± 0.415
0.0SerXaa: 0.0 ± 0.0
Thr
6.814ThrAla: 6.814 ± 0.682
0.277ThrCys: 0.277 ± 0.117
3.102ThrAsp: 3.102 ± 0.438
4.709ThrGlu: 4.709 ± 0.49
1.828ThrPhe: 1.828 ± 0.278
4.543ThrGly: 4.543 ± 0.505
0.997ThrHis: 0.997 ± 0.253
5.263ThrIle: 5.263 ± 0.571
3.767ThrLys: 3.767 ± 0.524
5.928ThrLeu: 5.928 ± 0.571
1.385ThrMet: 1.385 ± 0.24
2.216ThrAsn: 2.216 ± 0.258
3.435ThrPro: 3.435 ± 0.446
2.105ThrGln: 2.105 ± 0.428
3.546ThrArg: 3.546 ± 0.592
4.432ThrSer: 4.432 ± 0.481
5.152ThrThr: 5.152 ± 0.608
4.764ThrVal: 4.764 ± 0.534
0.665ThrTrp: 0.665 ± 0.196
1.939ThrTyr: 1.939 ± 0.29
0.0ThrXaa: 0.0 ± 0.0
Val
6.703ValAla: 6.703 ± 0.434
0.609ValCys: 0.609 ± 0.195
4.432ValAsp: 4.432 ± 0.373
5.097ValGlu: 5.097 ± 0.572
2.271ValPhe: 2.271 ± 0.323
5.374ValGly: 5.374 ± 0.741
0.886ValHis: 0.886 ± 0.197
4.155ValIle: 4.155 ± 0.467
4.598ValLys: 4.598 ± 0.453
5.207ValLeu: 5.207 ± 0.598
1.939ValMet: 1.939 ± 0.419
2.493ValAsn: 2.493 ± 0.429
2.881ValPro: 2.881 ± 0.48
1.994ValGln: 1.994 ± 0.352
4.82ValArg: 4.82 ± 0.597
4.93ValSer: 4.93 ± 0.552
4.764ValThr: 4.764 ± 0.626
5.817ValVal: 5.817 ± 0.708
0.942ValTrp: 0.942 ± 0.228
3.213ValTyr: 3.213 ± 0.512
0.0ValXaa: 0.0 ± 0.0
Trp
0.997TrpAla: 0.997 ± 0.267
0.222TrpCys: 0.222 ± 0.1
0.665TrpAsp: 0.665 ± 0.194
0.942TrpGlu: 0.942 ± 0.24
0.443TrpPhe: 0.443 ± 0.129
1.108TrpGly: 1.108 ± 0.265
0.388TrpHis: 0.388 ± 0.125
0.609TrpIle: 0.609 ± 0.181
0.609TrpLys: 0.609 ± 0.168
0.886TrpLeu: 0.886 ± 0.212
0.332TrpMet: 0.332 ± 0.126
0.997TrpAsn: 0.997 ± 0.212
0.554TrpPro: 0.554 ± 0.192
0.388TrpGln: 0.388 ± 0.123
0.72TrpArg: 0.72 ± 0.185
1.163TrpSer: 1.163 ± 0.257
0.776TrpThr: 0.776 ± 0.16
0.997TrpVal: 0.997 ± 0.173
0.111TrpTrp: 0.111 ± 0.081
0.609TrpTyr: 0.609 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.324TyrAla: 3.324 ± 0.488
0.332TyrCys: 0.332 ± 0.138
2.105TyrAsp: 2.105 ± 0.444
2.161TyrGlu: 2.161 ± 0.361
1.053TyrPhe: 1.053 ± 0.273
2.881TyrGly: 2.881 ± 0.402
0.776TyrHis: 0.776 ± 0.223
1.884TyrIle: 1.884 ± 0.307
2.105TyrLys: 2.105 ± 0.411
2.715TyrLeu: 2.715 ± 0.34
0.609TyrMet: 0.609 ± 0.183
1.33TyrAsn: 1.33 ± 0.192
1.496TyrPro: 1.496 ± 0.297
1.053TyrGln: 1.053 ± 0.207
1.717TyrArg: 1.717 ± 0.343
2.548TyrSer: 2.548 ± 0.454
2.216TyrThr: 2.216 ± 0.399
2.438TyrVal: 2.438 ± 0.309
0.443TyrTrp: 0.443 ± 0.144
0.942TyrTyr: 0.942 ± 0.28
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (18052 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski