Bacteroides sp. An322

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; unclassified Bacteroides

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2802 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4AYL8|A0A1Y4AYL8_9BACE Uncharacterized protein OS=Bacteroides sp. An322 OX=1965632 GN=B5F91_01625 PE=4 SV=1
MM1 pKa = 7.85CIGLFSCEE9 pKa = 3.78KK10 pKa = 10.74DD11 pKa = 3.52EE12 pKa = 5.47VITEE16 pKa = 4.29VPEE19 pKa = 4.02PTPEE23 pKa = 3.96EE24 pKa = 4.19PAPEE28 pKa = 4.59PEE30 pKa = 3.93PAHH33 pKa = 6.06YY34 pKa = 10.99VDD36 pKa = 3.47ITYY39 pKa = 9.14TLNCSEE45 pKa = 5.43DD46 pKa = 3.41LLKK49 pKa = 11.03YY50 pKa = 9.36VIPQVTYY57 pKa = 9.69MGDD60 pKa = 3.25DD61 pKa = 3.61GKK63 pKa = 11.11EE64 pKa = 3.8VTVQITDD71 pKa = 3.73TDD73 pKa = 3.17WMQINNDD80 pKa = 2.86ARR82 pKa = 11.84FGVTFGDD89 pKa = 4.48GSSSSANIMTWTRR102 pKa = 11.84KK103 pKa = 8.68LHH105 pKa = 5.48WEE107 pKa = 4.1EE108 pKa = 4.72FPVDD112 pKa = 3.57DD113 pKa = 5.4EE114 pKa = 5.37IIVSYY119 pKa = 10.49VPKK122 pKa = 10.23TDD124 pKa = 3.09MPEE127 pKa = 3.95YY128 pKa = 10.26EE129 pKa = 4.47KK130 pKa = 10.62TSVDD134 pKa = 4.01LFYY137 pKa = 11.37GDD139 pKa = 4.16YY140 pKa = 10.65SIAYY144 pKa = 9.01HH145 pKa = 6.66FVDD148 pKa = 4.62EE149 pKa = 4.74EE150 pKa = 4.14NGSIISQKK158 pKa = 11.08DD159 pKa = 3.17EE160 pKa = 4.29TGDD163 pKa = 2.94ISYY166 pKa = 8.7TLDD169 pKa = 3.25EE170 pKa = 4.6TKK172 pKa = 10.92EE173 pKa = 3.98EE174 pKa = 4.33LLDD177 pKa = 4.34LVDD180 pKa = 4.38KK181 pKa = 10.91TGEE184 pKa = 4.01PYY186 pKa = 10.37FWGTNGSNLIVGADD200 pKa = 2.94QDD202 pKa = 4.09MIEE205 pKa = 4.33EE206 pKa = 4.44VINLYY211 pKa = 8.23WRR213 pKa = 11.84KK214 pKa = 10.14LIIHH218 pKa = 6.22VDD220 pKa = 3.53GNGSYY225 pKa = 10.29SVSMKK230 pKa = 10.28IAGEE234 pKa = 4.12NEE236 pKa = 3.66

Molecular weight: 26.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4AC43|A0A1Y4AC43_9BACE Regulatory protein RecX OS=Bacteroides sp. An322 OX=1965632 GN=B5F91_07830 PE=3 SV=1
MM1 pKa = 7.69KK2 pKa = 10.4KK3 pKa = 10.23QILLLAALLALLTSCGTRR21 pKa = 11.84APHH24 pKa = 5.64YY25 pKa = 9.89DD26 pKa = 3.48YY27 pKa = 11.56RR28 pKa = 11.84EE29 pKa = 3.96LAQAALRR36 pKa = 11.84LDD38 pKa = 3.38IDD40 pKa = 3.52IDD42 pKa = 3.37MDD44 pKa = 4.67DD45 pKa = 3.24NHH47 pKa = 6.9RR48 pKa = 11.84LYY50 pKa = 10.34IEE52 pKa = 3.91SARR55 pKa = 11.84WIGVPYY61 pKa = 10.45RR62 pKa = 11.84SGGTTRR68 pKa = 11.84RR69 pKa = 11.84GVDD72 pKa = 3.15CSGFTSNIYY81 pKa = 8.5RR82 pKa = 11.84TVYY85 pKa = 10.06RR86 pKa = 11.84KK87 pKa = 9.6RR88 pKa = 11.84LKK90 pKa = 10.49RR91 pKa = 11.84SSDD94 pKa = 3.13RR95 pKa = 11.84QRR97 pKa = 11.84TEE99 pKa = 3.31DD100 pKa = 3.35CRR102 pKa = 11.84KK103 pKa = 9.16VRR105 pKa = 11.84KK106 pKa = 9.58AKK108 pKa = 10.33LRR110 pKa = 11.84EE111 pKa = 3.71GDD113 pKa = 4.1LVFFHH118 pKa = 6.95NGRR121 pKa = 11.84NKK123 pKa = 9.86RR124 pKa = 11.84RR125 pKa = 11.84ATHH128 pKa = 5.17VGIYY132 pKa = 10.36LKK134 pKa = 10.56DD135 pKa = 3.17GRR137 pKa = 11.84FIHH140 pKa = 6.98ASTSRR145 pKa = 11.84GVIVSCLNEE154 pKa = 4.12PYY156 pKa = 10.62YY157 pKa = 10.38KK158 pKa = 10.13RR159 pKa = 11.84CWMQGGRR166 pKa = 11.84VKK168 pKa = 10.89

Molecular weight: 19.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2802

0

2802

1004698

38

1981

358.6

40.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.309 ± 0.041

1.3 ± 0.017

5.622 ± 0.028

6.851 ± 0.044

4.488 ± 0.031

6.888 ± 0.041

1.959 ± 0.019

6.818 ± 0.041

6.291 ± 0.041

9.088 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.791 ± 0.022

4.89 ± 0.036

3.772 ± 0.024

3.559 ± 0.025

4.871 ± 0.037

5.752 ± 0.034

5.582 ± 0.037

6.402 ± 0.037

1.296 ± 0.021

4.471 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski