Amino acid dipepetide frequency for Bacteroides sp. An322

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.355AlaAla: 5.355 ± 0.093	1.122AlaCys: 1.122 ± 0.036	4.57AlaAsp: 4.57 ± 0.072	5.297AlaGlu: 5.297 ± 0.096	3.397AlaPhe: 3.397 ± 0.064	5.22AlaGly: 5.22 ± 0.084	1.419AlaHis: 1.419 ± 0.041	4.765AlaIle: 4.765 ± 0.069	4.052AlaLys: 4.052 ± 0.076	7.036AlaLeu: 7.036 ± 0.104	2.024AlaMet: 2.024 ± 0.053	3.184AlaAsn: 3.184 ± 0.063	2.278AlaPro: 2.278 ± 0.051	2.971AlaGln: 2.971 ± 0.064	3.313AlaArg: 3.313 ± 0.071	4.692AlaSer: 4.692 ± 0.081	3.616AlaThr: 3.616 ± 0.065	4.621AlaVal: 4.621 ± 0.071	0.891AlaTrp: 0.891 ± 0.033	3.262AlaTyr: 3.262 ± 0.064	0.0AlaXaa: 0.0 ± 0.0
Cys	0.872CysAla: 0.872 ± 0.027	0.217CysCys: 0.217 ± 0.017	0.65CysAsp: 0.65 ± 0.026	0.738CysGlu: 0.738 ± 0.03	0.657CysPhe: 0.657 ± 0.026	1.133CysGly: 1.133 ± 0.038	0.289CysHis: 0.289 ± 0.018	1.048CysIle: 1.048 ± 0.038	0.708CysLys: 0.708 ± 0.03	1.164CysLeu: 1.164 ± 0.039	0.368CysMet: 0.368 ± 0.016	0.565CysAsn: 0.565 ± 0.024	0.591CysPro: 0.591 ± 0.029	0.37CysGln: 0.37 ± 0.019	0.685CysArg: 0.685 ± 0.026	0.709CysSer: 0.709 ± 0.026	0.711CysThr: 0.711 ± 0.03	0.797CysVal: 0.797 ± 0.031	0.161CysTrp: 0.161 ± 0.013	0.573CysTyr: 0.573 ± 0.028	0.0CysXaa: 0.0 ± 0.0
Asp	4.4AspAla: 4.4 ± 0.07	0.633AspCys: 0.633 ± 0.028	2.644AspAsp: 2.644 ± 0.064	4.244AspGlu: 4.244 ± 0.07	3.067AspPhe: 3.067 ± 0.054	4.082AspGly: 4.082 ± 0.069	0.748AspHis: 0.748 ± 0.028	4.133AspIle: 4.133 ± 0.059	3.783AspLys: 3.783 ± 0.068	4.847AspLeu: 4.847 ± 0.082	1.743AspMet: 1.743 ± 0.036	2.716AspAsn: 2.716 ± 0.067	1.877AspPro: 1.877 ± 0.048	1.268AspGln: 1.268 ± 0.041	2.574AspArg: 2.574 ± 0.047	2.901AspSer: 2.901 ± 0.069	2.965AspThr: 2.965 ± 0.06	3.581AspVal: 3.581 ± 0.062	0.917AspTrp: 0.917 ± 0.032	3.091AspTyr: 3.091 ± 0.065	0.0AspXaa: 0.0 ± 0.0
Glu	5.503GluAla: 5.503 ± 0.092	0.71GluCys: 0.71 ± 0.026	3.607GluAsp: 3.607 ± 0.064	5.527GluGlu: 5.527 ± 0.103	2.373GluPhe: 2.373 ± 0.05	4.835GluGly: 4.835 ± 0.071	1.325GluHis: 1.325 ± 0.038	4.471GluIle: 4.471 ± 0.077	5.174GluLys: 5.174 ± 0.092	6.078GluLeu: 6.078 ± 0.092	2.187GluMet: 2.187 ± 0.047	3.535GluAsn: 3.535 ± 0.068	1.921GluPro: 1.921 ± 0.046	2.799GluGln: 2.799 ± 0.055	3.684GluArg: 3.684 ± 0.081	3.106GluSer: 3.106 ± 0.056	3.738GluThr: 3.738 ± 0.073	4.271GluVal: 4.271 ± 0.082	0.917GluTrp: 0.917 ± 0.03	2.75GluTyr: 2.75 ± 0.058	0.0GluXaa: 0.0 ± 0.0
Phe	3.132PheAla: 3.132 ± 0.069	0.681PheCys: 0.681 ± 0.028	2.855PheAsp: 2.855 ± 0.057	2.525PheGlu: 2.525 ± 0.048	2.094PhePhe: 2.094 ± 0.056	3.322PheGly: 3.322 ± 0.06	0.902PheHis: 0.902 ± 0.033	3.171PheIle: 3.171 ± 0.061	2.231PheLys: 2.231 ± 0.047	3.894PheLeu: 3.894 ± 0.075	1.179PheMet: 1.179 ± 0.033	2.249PheAsn: 2.249 ± 0.052	1.668PhePro: 1.668 ± 0.04	1.39PheGln: 1.39 ± 0.038	2.378PheArg: 2.378 ± 0.054	3.143PheSer: 3.143 ± 0.06	2.802PheThr: 2.802 ± 0.056	2.714PheVal: 2.714 ± 0.056	0.547PheTrp: 0.547 ± 0.026	2.003PheTyr: 2.003 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.398GlyAla: 4.398 ± 0.092	0.998GlyCys: 0.998 ± 0.03	3.314GlyAsp: 3.314 ± 0.063	4.477GlyGlu: 4.477 ± 0.065	3.278GlyPhe: 3.278 ± 0.064	4.759GlyGly: 4.759 ± 0.086	1.294GlyHis: 1.294 ± 0.041	5.57GlyIle: 5.57 ± 0.075	5.298GlyLys: 5.298 ± 0.081	5.842GlyLeu: 5.842 ± 0.083	2.282GlyMet: 2.282 ± 0.049	3.604GlyAsn: 3.604 ± 0.058	1.283GlyPro: 1.283 ± 0.036	2.015GlyGln: 2.015 ± 0.052	3.007GlyArg: 3.007 ± 0.048	3.787GlySer: 3.787 ± 0.077	4.375GlyThr: 4.375 ± 0.082	4.716GlyVal: 4.716 ± 0.063	1.106GlyTrp: 1.106 ± 0.04	3.474GlyTyr: 3.474 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	1.326HisAla: 1.326 ± 0.034	0.322HisCys: 0.322 ± 0.018	0.971HisAsp: 0.971 ± 0.032	1.135HisGlu: 1.135 ± 0.034	1.036HisPhe: 1.036 ± 0.036	1.2HisGly: 1.2 ± 0.031	0.512HisHis: 0.512 ± 0.025	1.562HisIle: 1.562 ± 0.041	0.99HisLys: 0.99 ± 0.033	1.846HisLeu: 1.846 ± 0.048	0.314HisMet: 0.314 ± 0.018	0.938HisAsn: 0.938 ± 0.032	1.167HisPro: 1.167 ± 0.039	0.631HisGln: 0.631 ± 0.025	1.005HisArg: 1.005 ± 0.033	1.056HisSer: 1.056 ± 0.034	1.238HisThr: 1.238 ± 0.034	1.139HisVal: 1.139 ± 0.038	0.265HisTrp: 0.265 ± 0.016	0.934HisTyr: 0.934 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.445IleAla: 5.445 ± 0.079	0.968IleCys: 0.968 ± 0.036	4.493IleAsp: 4.493 ± 0.076	4.637IleGlu: 4.637 ± 0.066	2.534IlePhe: 2.534 ± 0.056	4.906IleGly: 4.906 ± 0.084	1.422IleHis: 1.422 ± 0.039	4.422IleIle: 4.422 ± 0.08	3.765IleLys: 3.765 ± 0.064	5.733IleLeu: 5.733 ± 0.095	1.406IleMet: 1.406 ± 0.039	3.235IleAsn: 3.235 ± 0.061	3.108IlePro: 3.108 ± 0.058	2.339IleGln: 2.339 ± 0.056	3.797IleArg: 3.797 ± 0.054	4.214IleSer: 4.214 ± 0.072	3.908IleThr: 3.908 ± 0.069	4.416IleVal: 4.416 ± 0.079	0.706IleTrp: 0.706 ± 0.03	2.72IleTyr: 2.72 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	4.888LysAla: 4.888 ± 0.091	0.519LysCys: 0.519 ± 0.024	3.799LysAsp: 3.799 ± 0.066	5.68LysGlu: 5.68 ± 0.084	2.078LysPhe: 2.078 ± 0.044	4.38LysGly: 4.38 ± 0.065	1.32LysHis: 1.32 ± 0.031	3.73LysIle: 3.73 ± 0.067	4.623LysLys: 4.623 ± 0.075	5.247LysLeu: 5.247 ± 0.068	1.89LysMet: 1.89 ± 0.04	3.128LysAsn: 3.128 ± 0.059	2.164LysPro: 2.164 ± 0.046	2.599LysGln: 2.599 ± 0.057	3.133LysArg: 3.133 ± 0.059	2.973LysSer: 2.973 ± 0.058	3.342LysThr: 3.342 ± 0.054	3.964LysVal: 3.964 ± 0.073	0.699LysTrp: 0.699 ± 0.029	2.752LysTyr: 2.752 ± 0.055	0.0LysXaa: 0.0 ± 0.0
Leu	6.842LeuAla: 6.842 ± 0.095	1.442LeuCys: 1.442 ± 0.042	4.847LeuAsp: 4.847 ± 0.067	5.195LeuGlu: 5.195 ± 0.088	4.344LeuPhe: 4.344 ± 0.07	5.568LeuGly: 5.568 ± 0.095	1.944LeuHis: 1.944 ± 0.047	5.682LeuIle: 5.682 ± 0.097	6.059LeuLys: 6.059 ± 0.081	8.432LeuLeu: 8.432 ± 0.122	2.534LeuMet: 2.534 ± 0.058	4.477LeuAsn: 4.477 ± 0.076	4.103LeuPro: 4.103 ± 0.064	3.385LeuGln: 3.385 ± 0.06	4.446LeuArg: 4.446 ± 0.071	6.326LeuSer: 6.326 ± 0.089	5.318LeuThr: 5.318 ± 0.079	5.247LeuVal: 5.247 ± 0.084	1.026LeuTrp: 1.026 ± 0.034	3.661LeuTyr: 3.661 ± 0.059	0.0LeuXaa: 0.0 ± 0.0
Met	2.097MetAla: 2.097 ± 0.057	0.294MetCys: 0.294 ± 0.018	1.585MetAsp: 1.585 ± 0.042	1.878MetGlu: 1.878 ± 0.045	1.028MetPhe: 1.028 ± 0.034	1.796MetGly: 1.796 ± 0.05	0.504MetHis: 0.504 ± 0.024	1.529MetIle: 1.529 ± 0.043	2.611MetLys: 2.611 ± 0.045	2.59MetLeu: 2.59 ± 0.058	0.829MetMet: 0.829 ± 0.029	1.671MetAsn: 1.671 ± 0.042	1.257MetPro: 1.257 ± 0.033	1.137MetGln: 1.137 ± 0.038	1.438MetArg: 1.438 ± 0.034	1.478MetSer: 1.478 ± 0.035	1.475MetThr: 1.475 ± 0.038	1.555MetVal: 1.555 ± 0.046	0.235MetTrp: 0.235 ± 0.016	0.923MetTyr: 0.923 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	3.582AsnAla: 3.582 ± 0.065	0.513AsnCys: 0.513 ± 0.025	2.525AsnAsp: 2.525 ± 0.057	3.237AsnGlu: 3.237 ± 0.06	2.08AsnPhe: 2.08 ± 0.058	3.878AsnGly: 3.878 ± 0.076	0.927AsnHis: 0.927 ± 0.03	3.496AsnIle: 3.496 ± 0.073	2.796AsnLys: 2.796 ± 0.052	4.305AsnLeu: 4.305 ± 0.078	1.277AsnMet: 1.277 ± 0.032	2.412AsnAsn: 2.412 ± 0.058	2.545AsnPro: 2.545 ± 0.052	1.688AsnGln: 1.688 ± 0.042	2.504AsnArg: 2.504 ± 0.056	2.317AsnSer: 2.317 ± 0.05	2.582AsnThr: 2.582 ± 0.062	3.227AsnVal: 3.227 ± 0.059	0.678AsnTrp: 0.678 ± 0.031	2.337AsnTyr: 2.337 ± 0.057	0.0AsnXaa: 0.0 ± 0.0
Pro	2.751ProAla: 2.751 ± 0.055	0.414ProCys: 0.414 ± 0.02	2.762ProAsp: 2.762 ± 0.056	3.636ProGlu: 3.636 ± 0.055	1.816ProPhe: 1.816 ± 0.045	2.271ProGly: 2.271 ± 0.043	0.791ProHis: 0.791 ± 0.029	2.349ProIle: 2.349 ± 0.046	1.977ProLys: 1.977 ± 0.049	3.373ProLeu: 3.373 ± 0.057	0.966ProMet: 0.966 ± 0.03	1.734ProAsn: 1.734 ± 0.047	0.831ProPro: 0.831 ± 0.03	1.479ProGln: 1.479 ± 0.041	1.283ProArg: 1.283 ± 0.039	2.163ProSer: 2.163 ± 0.048	1.904ProThr: 1.904 ± 0.047	2.915ProVal: 2.915 ± 0.059	0.487ProTrp: 0.487 ± 0.027	1.821ProTyr: 1.821 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	2.927GlnAla: 2.927 ± 0.066	0.366GlnCys: 0.366 ± 0.018	1.678GlnAsp: 1.678 ± 0.046	2.514GlnGlu: 2.514 ± 0.053	1.36GlnPhe: 1.36 ± 0.036	2.176GlnGly: 2.176 ± 0.04	0.743GlnHis: 0.743 ± 0.029	2.451GlnIle: 2.451 ± 0.053	2.299GlnLys: 2.299 ± 0.048	3.338GlnLeu: 3.338 ± 0.061	1.076GlnMet: 1.076 ± 0.033	1.748GlnAsn: 1.748 ± 0.047	1.288GlnPro: 1.288 ± 0.034	1.556GlnGln: 1.556 ± 0.05	1.724GlnArg: 1.724 ± 0.045	1.853GlnSer: 1.853 ± 0.041	2.178GlnThr: 2.178 ± 0.049	2.332GlnVal: 2.332 ± 0.05	0.479GlnTrp: 0.479 ± 0.024	1.5GlnTyr: 1.5 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	3.082ArgAla: 3.082 ± 0.062	0.525ArgCys: 0.525 ± 0.023	2.21ArgAsp: 2.21 ± 0.049	3.274ArgGlu: 3.274 ± 0.067	2.433ArgPhe: 2.433 ± 0.051	2.475ArgGly: 2.475 ± 0.055	1.024ArgHis: 1.024 ± 0.035	3.895ArgIle: 3.895 ± 0.073	3.599ArgLys: 3.599 ± 0.064	4.811ArgLeu: 4.811 ± 0.084	1.745ArgMet: 1.745 ± 0.039	2.481ArgAsn: 2.481 ± 0.05	1.755ArgPro: 1.755 ± 0.048	1.924ArgGln: 1.924 ± 0.049	2.567ArgArg: 2.567 ± 0.065	2.513ArgSer: 2.513 ± 0.054	2.633ArgThr: 2.633 ± 0.056	2.695ArgVal: 2.695 ± 0.054	0.712ArgTrp: 0.712 ± 0.03	2.359ArgTyr: 2.359 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	4.058SerAla: 4.058 ± 0.068	0.801SerCys: 0.801 ± 0.031	3.291SerAsp: 3.291 ± 0.055	3.511SerGlu: 3.511 ± 0.056	3.03SerPhe: 3.03 ± 0.057	4.492SerGly: 4.492 ± 0.068	1.065SerHis: 1.065 ± 0.031	4.02SerIle: 4.02 ± 0.07	2.979SerLys: 2.979 ± 0.058	5.701SerLeu: 5.701 ± 0.077	1.488SerMet: 1.488 ± 0.036	2.408SerAsn: 2.408 ± 0.057	2.258SerPro: 2.258 ± 0.047	1.939SerGln: 1.939 ± 0.047	2.612SerArg: 2.612 ± 0.055	3.402SerSer: 3.402 ± 0.073	2.953SerThr: 2.953 ± 0.06	4.117SerVal: 4.117 ± 0.065	0.781SerTrp: 0.781 ± 0.035	2.62SerTyr: 2.62 ± 0.057	0.0SerXaa: 0.0 ± 0.0
Thr	3.955ThrAla: 3.955 ± 0.066	0.631ThrCys: 0.631 ± 0.023	3.635ThrAsp: 3.635 ± 0.067	3.51ThrGlu: 3.51 ± 0.06	2.746ThrPhe: 2.746 ± 0.053	4.405ThrGly: 4.405 ± 0.073	1.079ThrHis: 1.079 ± 0.038	3.742ThrIle: 3.742 ± 0.07	2.472ThrLys: 2.472 ± 0.051	5.617ThrLeu: 5.617 ± 0.075	1.239ThrMet: 1.239 ± 0.037	2.351ThrAsn: 2.351 ± 0.055	2.826ThrPro: 2.826 ± 0.054	1.904ThrGln: 1.904 ± 0.048	2.317ThrArg: 2.317 ± 0.052	3.247ThrSer: 3.247 ± 0.059	2.876ThrThr: 2.876 ± 0.064	4.074ThrVal: 4.074 ± 0.069	0.671ThrTrp: 0.671 ± 0.027	2.527ThrTyr: 2.527 ± 0.058	0.0ThrXaa: 0.0 ± 0.0
Val	4.343ValAla: 4.343 ± 0.075	1.095ValCys: 1.095 ± 0.036	3.549ValAsp: 3.549 ± 0.072	4.059ValGlu: 4.059 ± 0.072	2.906ValPhe: 2.906 ± 0.057	3.995ValGly: 3.995 ± 0.065	1.1ValHis: 1.1 ± 0.03	4.362ValIle: 4.362 ± 0.067	4.098ValLys: 4.098 ± 0.064	5.838ValLeu: 5.838 ± 0.083	1.71ValMet: 1.71 ± 0.048	3.216ValAsn: 3.216 ± 0.054	2.603ValPro: 2.603 ± 0.06	1.942ValGln: 1.942 ± 0.05	3.207ValArg: 3.207 ± 0.062	4.523ValSer: 4.523 ± 0.074	3.673ValThr: 3.673 ± 0.068	4.248ValVal: 4.248 ± 0.072	0.812ValTrp: 0.812 ± 0.027	2.747ValTyr: 2.747 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.824TrpAla: 0.824 ± 0.033	0.167TrpCys: 0.167 ± 0.014	0.715TrpAsp: 0.715 ± 0.026	0.83TrpGlu: 0.83 ± 0.03	0.579TrpPhe: 0.579 ± 0.025	0.976TrpGly: 0.976 ± 0.034	0.291TrpHis: 0.291 ± 0.019	0.866TrpIle: 0.866 ± 0.03	0.919TrpLys: 0.919 ± 0.029	1.228TrpLeu: 1.228 ± 0.035	0.493TrpMet: 0.493 ± 0.021	0.834TrpAsn: 0.834 ± 0.031	0.226TrpPro: 0.226 ± 0.017	0.536TrpGln: 0.536 ± 0.024	0.582TrpArg: 0.582 ± 0.023	0.714TrpSer: 0.714 ± 0.031	0.706TrpThr: 0.706 ± 0.029	0.706TrpVal: 0.706 ± 0.026	0.234TrpTrp: 0.234 ± 0.017	0.531TrpTyr: 0.531 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.304TyrAla: 3.304 ± 0.063	0.587TyrCys: 0.587 ± 0.026	2.517TyrAsp: 2.517 ± 0.051	2.609TyrGlu: 2.609 ± 0.049	2.045TyrPhe: 2.045 ± 0.05	3.006TyrGly: 3.006 ± 0.061	0.889TyrHis: 0.889 ± 0.034	2.913TyrIle: 2.913 ± 0.054	2.478TyrLys: 2.478 ± 0.05	3.957TyrLeu: 3.957 ± 0.067	1.157TyrMet: 1.157 ± 0.038	2.418TyrAsn: 2.418 ± 0.06	1.976TyrPro: 1.976 ± 0.05	1.714TyrGln: 1.714 ± 0.047	2.453TyrArg: 2.453 ± 0.05	2.407TyrSer: 2.407 ± 0.054	2.832TyrThr: 2.832 ± 0.068	2.688TyrVal: 2.688 ± 0.055	0.634TyrTrp: 0.634 ± 0.028	2.126TyrTyr: 2.126 ± 0.05	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2802 proteins (1004698 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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