Ferrimonas sediminum
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3939 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G8Q5R6|A0A1G8Q5R6_9GAMM Positive regulator of sigma(E) RseC/MucC OS=Ferrimonas sediminum OX=718193 GN=SAMN04488540_104191 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 10.12 RR3 pKa = 11.84 HH4 pKa = 5.46 KK5 pKa = 9.35 TNAITRR11 pKa = 11.84 TLGIALVGTAGLYY24 pKa = 10.63 CLVSPTTARR33 pKa = 11.84 ADD35 pKa = 3.81 FFPQQLNQACMAEE48 pKa = 3.81 AAGFNLNCTANDD60 pKa = 3.4 IQVSQVTNITRR71 pKa = 11.84 PDD73 pKa = 3.62 GSPGPVEE80 pKa = 4.07 CTLGGDD86 pKa = 3.61 AEE88 pKa = 4.33 FRR90 pKa = 11.84 ADD92 pKa = 3.45 VTIVTTAKK100 pKa = 9.84 RR101 pKa = 11.84 RR102 pKa = 11.84 YY103 pKa = 9.63 DD104 pKa = 3.43 YY105 pKa = 10.79 TVYY108 pKa = 10.54 LPEE111 pKa = 5.14 GNWSPQEE118 pKa = 3.98 FNTSNTCSVLVGQDD132 pKa = 3.03 FTAPGEE138 pKa = 4.13 DD139 pKa = 4.33 LEE141 pKa = 5.99 DD142 pKa = 4.72 PGDD145 pKa = 3.64 MCADD149 pKa = 3.2 ISKK152 pKa = 10.26 PPGSHH157 pKa = 7.53 LYY159 pKa = 7.88 TQQLITLNCVDD170 pKa = 4.25 EE171 pKa = 5.03 DD172 pKa = 3.74 QDD174 pKa = 3.31 GRR176 pKa = 11.84 AEE178 pKa = 4.0 FRR180 pKa = 11.84 YY181 pKa = 9.64 CAAWDD186 pKa = 3.74 NKK188 pKa = 10.68 ADD190 pKa = 3.85 STPVCDD196 pKa = 3.85 GTAATTPVPGTPSKK210 pKa = 10.7 CRR212 pKa = 11.84 CDD214 pKa = 3.58 EE215 pKa = 4.04 FNIDD219 pKa = 4.19 VFIKK223 pKa = 10.28 PDD225 pKa = 3.62 PPTINKK231 pKa = 9.74 SEE233 pKa = 4.11 GSPITRR239 pKa = 11.84 PEE241 pKa = 3.88 PGGDD245 pKa = 3.46 YY246 pKa = 10.71 TFTASFTNNSLTSIFIDD263 pKa = 3.74 SVADD267 pKa = 4.44 EE268 pKa = 4.53 IDD270 pKa = 3.38 VGGEE274 pKa = 3.92 GSFDD278 pKa = 3.55 VSLDD282 pKa = 3.69 LLNGATQTVDD292 pKa = 3.26 PTNTATAEE300 pKa = 4.12 GVYY303 pKa = 10.23 LKK305 pKa = 10.72 SLSASCAQPADD316 pKa = 3.42 IGNGAGEE323 pKa = 4.44 LVSGATYY330 pKa = 10.63 SCTFTVTIVDD340 pKa = 3.46 RR341 pKa = 11.84 DD342 pKa = 3.82 LPNDD346 pKa = 3.63 QSPEE350 pKa = 3.99 LYY352 pKa = 10.42 KK353 pKa = 10.95 DD354 pKa = 3.68 VIKK357 pKa = 11.06 VALTDD362 pKa = 3.6 KK363 pKa = 11.05 NGNPVVNGATCPADD377 pKa = 3.92 LSPIAGDD384 pKa = 3.29 HH385 pKa = 6.26 CSVLRR390 pKa = 11.84 TVDD393 pKa = 3.52 VTNLPPSITVSKK405 pKa = 9.51 TPSPDD410 pKa = 3.06 QVLEE414 pKa = 4.09 PGGDD418 pKa = 3.33 VTFDD422 pKa = 3.0 IVVTSTSGNYY432 pKa = 9.69 DD433 pKa = 3.51 DD434 pKa = 5.89 PLTLTSLMDD443 pKa = 3.67 TVFGDD448 pKa = 3.75 LNGVGTCATGGSFSLAAPYY467 pKa = 10.35 SCSFTEE473 pKa = 4.78 TISGNAGDD481 pKa = 3.79 VHH483 pKa = 7.0 NNTVTAKK490 pKa = 9.97 ATDD493 pKa = 3.69 NEE495 pKa = 3.93 NDD497 pKa = 3.23 EE498 pKa = 4.66 AQNSDD503 pKa = 3.16 GATVNINDD511 pKa = 3.72 VPSIITLVKK520 pKa = 8.67 TADD523 pKa = 4.43 PIEE526 pKa = 4.16 VDD528 pKa = 3.57 EE529 pKa = 5.05 TGDD532 pKa = 3.64 DD533 pKa = 3.47 PTVFRR538 pKa = 11.84 DD539 pKa = 3.0 VDD541 pKa = 4.04 FTFEE545 pKa = 4.08 FSVDD549 pKa = 3.1 AAGVDD554 pKa = 3.55 DD555 pKa = 3.95 VTFNSLTDD563 pKa = 3.95 TIFGTLTGEE572 pKa = 4.41 CLVDD576 pKa = 3.6 TKK578 pKa = 10.95 NGSPIGSTPLSGFVLQPGEE597 pKa = 4.45 DD598 pKa = 3.61 ASCTITKK605 pKa = 10.14 ALQGDD610 pKa = 3.82 AGDD613 pKa = 3.61 VHH615 pKa = 7.69 NNVATIKK622 pKa = 11.11 GIDD625 pKa = 3.82 EE626 pKa = 4.52 DD627 pKa = 4.32 AQPVMDD633 pKa = 5.29 SDD635 pKa = 4.19 DD636 pKa = 3.68 ATVTFLDD643 pKa = 3.71 TGLDD647 pKa = 3.63 MEE649 pKa = 4.74 QEE651 pKa = 4.44 FAAKK655 pKa = 9.45 MIAFVRR661 pKa = 11.84 ITNGNVDD668 pKa = 3.68 TASITGVTIKK678 pKa = 10.61 GVNLVAGGGIPGQFEE693 pKa = 4.33 VQNDD697 pKa = 3.92 AGTSSYY703 pKa = 11.54 NPGDD707 pKa = 3.56 GPYY710 pKa = 9.83 AFCSTGVDD718 pKa = 3.31 ILPGDD723 pKa = 3.83 TYY725 pKa = 11.69 EE726 pKa = 4.9 CAFTLKK732 pKa = 10.12 LFPGFPPGDD741 pKa = 3.52 INFSATGANGLVFTYY756 pKa = 10.51 QDD758 pKa = 3.38 NEE760 pKa = 4.48 GNSVTTNVDD769 pKa = 3.5 MNVMTIEE776 pKa = 3.87
Molecular weight: 81.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.732
IPC_protein 3.783
Toseland 3.554
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.732
Rodwell 3.605
Grimsley 3.452
Solomon 3.783
Lehninger 3.745
Nozaki 3.884
DTASelect 4.177
Thurlkill 3.605
EMBOSS 3.745
Sillero 3.91
Patrickios 1.888
IPC_peptide 3.783
IPC2_peptide 3.884
IPC2.peptide.svr19 3.808
Protein with the highest isoelectric point:
>tr|A0A1G8PSR4|A0A1G8PSR4_9GAMM Deoxyribose-phosphate aldolase OS=Ferrimonas sediminum OX=718193 GN=deoC PE=3 SV=1
MM1 pKa = 7.35 NLKK4 pKa = 10.01 FWQRR8 pKa = 11.84 PPLQASPQRR17 pKa = 11.84 TLGHH21 pKa = 6.49 NDD23 pKa = 2.75 ILILPTRR30 pKa = 11.84 FGVLFLGLSLVLFLFGSNYY49 pKa = 9.41 QNNLILMLAFLMVSLFSSCLLICYY73 pKa = 9.73 RR74 pKa = 11.84 NLAGVTLSPQPAPGTHH90 pKa = 6.69 AGDD93 pKa = 3.7 PARR96 pKa = 11.84 FPISLSGTGHH106 pKa = 6.66 SYY108 pKa = 10.8 HH109 pKa = 6.42 LQLGFVQGDD118 pKa = 3.54 KK119 pKa = 10.94 QLLHH123 pKa = 6.38 TLSATPSRR131 pKa = 11.84 LEE133 pKa = 3.88 VAWPSRR139 pKa = 11.84 RR140 pKa = 11.84 RR141 pKa = 11.84 GPLLPPPLIISSRR154 pKa = 11.84 YY155 pKa = 8.4 PLGLCRR161 pKa = 11.84 VWSRR165 pKa = 11.84 LALNQAAWVWPRR177 pKa = 11.84 PLAGDD182 pKa = 3.97 TPQAHH187 pKa = 7.39 RR188 pKa = 11.84 PRR190 pKa = 11.84 QEE192 pKa = 3.72 QGDD195 pKa = 3.82 KK196 pKa = 10.76 HH197 pKa = 6.22 QSNSNDD203 pKa = 3.25 FDD205 pKa = 4.16 GLKK208 pKa = 9.69 PWQRR212 pKa = 11.84 GHH214 pKa = 6.04 SLGRR218 pKa = 11.84 VAWKK222 pKa = 10.18 QLAQQRR228 pKa = 11.84 GMLVKK233 pKa = 10.59 QFDD236 pKa = 4.14 SPGPNPEE243 pKa = 4.74 LLTVDD248 pKa = 3.3 NRR250 pKa = 11.84 HH251 pKa = 6.12 PQLEE255 pKa = 4.32 HH256 pKa = 6.76 HH257 pKa = 6.54 LSEE260 pKa = 4.62 LAGRR264 pKa = 11.84 LHH266 pKa = 7.33 RR267 pKa = 11.84 LHH269 pKa = 5.79 QQGVPVGLSTDD280 pKa = 3.64 TEE282 pKa = 4.58 TLMPALGRR290 pKa = 11.84 THH292 pKa = 7.26 LHH294 pKa = 6.07 HH295 pKa = 7.23 CLTLLARR302 pKa = 11.84 VEE304 pKa = 4.18 PHH306 pKa = 6.67 ASS308 pKa = 3.09
Molecular weight: 34.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.458
IPC_protein 10.262
Toseland 10.438
ProMoST 10.175
Dawson 10.57
Bjellqvist 10.306
Wikipedia 10.789
Rodwell 10.687
Grimsley 10.628
Solomon 10.672
Lehninger 10.643
Nozaki 10.467
DTASelect 10.292
Thurlkill 10.452
EMBOSS 10.847
Sillero 10.511
Patrickios 10.394
IPC_peptide 10.672
IPC2_peptide 9.545
IPC2.peptide.svr19 8.532
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3939
0
3939
1277342
18
2705
324.3
35.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.819 ± 0.052
1.163 ± 0.016
5.657 ± 0.035
5.722 ± 0.036
3.66 ± 0.026
7.606 ± 0.035
2.396 ± 0.025
5.008 ± 0.026
3.908 ± 0.037
11.256 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.694 ± 0.02
3.401 ± 0.026
4.437 ± 0.027
5.274 ± 0.038
5.617 ± 0.033
6.152 ± 0.027
5.043 ± 0.026
7.029 ± 0.034
1.387 ± 0.017
2.772 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here