Pseudomonas phage KPP25 (Bacteriophage KPP25)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kochitakasuvirus; Pseudomonas virus KPP25

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 88 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X5HZQ0|X5HZQ0_BPKP2 Uncharacterized protein OS=Pseudomonas phage KPP25 OX=1462608 PE=4 SV=1
MM1 pKa = 7.58AVTPEE6 pKa = 4.03LVYY9 pKa = 11.1DD10 pKa = 3.93GVGFVSNLVTDD21 pKa = 4.74GNQRR25 pKa = 11.84MLVLVGRR32 pKa = 11.84TQFSLNVLYY41 pKa = 11.25SNDD44 pKa = 4.87AGVTWNSTGLPPAINPGDD62 pKa = 3.57LALINGSLTDD72 pKa = 3.36GHH74 pKa = 6.72LTLTSSGYY82 pKa = 8.65TPGGQYY88 pKa = 10.79VSYY91 pKa = 10.23LFCSDD96 pKa = 3.88DD97 pKa = 5.11AGMSWSEE104 pKa = 3.69VAMPSGYY111 pKa = 10.42RR112 pKa = 11.84NFVTAGYY119 pKa = 9.29DD120 pKa = 2.99ITMGYY125 pKa = 8.98YY126 pKa = 8.36WFVVEE131 pKa = 4.67MQSSPWNRR139 pKa = 11.84AIYY142 pKa = 8.09RR143 pKa = 11.84TQDD146 pKa = 3.39LVDD149 pKa = 4.36LEE151 pKa = 5.16LIQMLPGNQEE161 pKa = 3.56GFTIQTFYY169 pKa = 11.47NNKK172 pKa = 9.67EE173 pKa = 4.2GALSWAGRR181 pKa = 11.84NAWNSSNGGNLLTGGIVGSSILTHH205 pKa = 5.39VEE207 pKa = 3.96YY208 pKa = 9.66PIVPATTYY216 pKa = 10.29FYY218 pKa = 11.21DD219 pKa = 3.6SLVICKK225 pKa = 8.97PAYY228 pKa = 9.75PNDD231 pKa = 3.55GQIYY235 pKa = 10.16LGIDD239 pKa = 3.03TSTYY243 pKa = 9.73EE244 pKa = 3.88IAVNAQQPNPAPAAYY259 pKa = 9.85FDD261 pKa = 4.32GEE263 pKa = 4.37VGIVSARR270 pKa = 11.84ITNDD274 pKa = 2.25GSNFFDD280 pKa = 4.41SYY282 pKa = 10.07FARR285 pKa = 11.84IGGTWQQTSPLPTNQQPVAFVKK307 pKa = 10.92GLDD310 pKa = 3.5DD311 pKa = 5.62LIYY314 pKa = 10.86VLGNNGILSSTVIGATFTQEE334 pKa = 3.81YY335 pKa = 9.17TGFPSSGVLSPLMGKK350 pKa = 8.77VQDD353 pKa = 4.12GLCVLFDD360 pKa = 3.33NGQIYY365 pKa = 9.56RR366 pKa = 11.84VPLLPPEE373 pKa = 4.39PPVVGKK379 pKa = 9.61FWTDD383 pKa = 2.59IEE385 pKa = 4.45YY386 pKa = 11.03CEE388 pKa = 4.29EE389 pKa = 4.01FF390 pKa = 4.07

Molecular weight: 42.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X5I375|X5I375_BPKP2 Uncharacterized protein OS=Pseudomonas phage KPP25 OX=1462608 PE=4 SV=1
MM1 pKa = 7.04STFAALKK8 pKa = 9.7NLGSAIVSDD17 pKa = 3.98IAAGARR23 pKa = 11.84ATASATSSAYY33 pKa = 9.78EE34 pKa = 4.09SVSAKK39 pKa = 9.55VAHH42 pKa = 6.62KK43 pKa = 10.32RR44 pKa = 11.84GEE46 pKa = 3.84RR47 pKa = 11.84QIINEE52 pKa = 4.04FRR54 pKa = 11.84NMLNEE59 pKa = 4.17HH60 pKa = 6.7PGEE63 pKa = 4.04LLAAMRR69 pKa = 11.84SIQDD73 pKa = 3.45QLAQPAKK80 pKa = 9.6PARR83 pKa = 11.84KK84 pKa = 8.55PRR86 pKa = 11.84ARR88 pKa = 11.84KK89 pKa = 8.7AAAQQ93 pKa = 3.42

Molecular weight: 9.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

88

0

88

19981

42

2009

227.1

25.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.61 ± 0.453

1.031 ± 0.168

6.516 ± 0.209

6.191 ± 0.329

3.438 ± 0.198

6.831 ± 0.36

1.677 ± 0.153

4.98 ± 0.204

4.529 ± 0.39

8.568 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.698 ± 0.131

3.939 ± 0.191

5.055 ± 0.199

4.244 ± 0.294

6.676 ± 0.369

5.71 ± 0.209

6.256 ± 0.226

6.746 ± 0.23

1.316 ± 0.104

2.988 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski