Amino acid dipepetide frequency for Pseudomonas phage KPP25 (Bacteriophage KPP25)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.764AlaAla: 13.764 ± 1.434
0.651AlaCys: 0.651 ± 0.21
6.807AlaAsp: 6.807 ± 0.699
6.356AlaGlu: 6.356 ± 0.751
3.053AlaPhe: 3.053 ± 0.396
7.407AlaGly: 7.407 ± 0.838
1.502AlaHis: 1.502 ± 0.3
5.105AlaIle: 5.105 ± 0.553
5.656AlaLys: 5.656 ± 0.718
9.409AlaLeu: 9.409 ± 0.775
3.153AlaMet: 3.153 ± 0.44
3.854AlaAsn: 3.854 ± 0.414
3.954AlaPro: 3.954 ± 0.618
5.856AlaGln: 5.856 ± 1.11
7.357AlaArg: 7.357 ± 0.808
5.105AlaSer: 5.105 ± 0.643
6.707AlaThr: 6.707 ± 0.771
6.807AlaVal: 6.807 ± 0.644
1.101AlaTrp: 1.101 ± 0.246
2.452AlaTyr: 2.452 ± 0.295
0.0AlaXaa: 0.0 ± 0.0
Cys
0.701CysAla: 0.701 ± 0.189
0.05CysCys: 0.05 ± 0.05
0.651CysAsp: 0.651 ± 0.178
0.951CysGlu: 0.951 ± 0.286
0.45CysPhe: 0.45 ± 0.157
0.951CysGly: 0.951 ± 0.23
0.3CysHis: 0.3 ± 0.122
0.45CysIle: 0.45 ± 0.147
0.3CysLys: 0.3 ± 0.109
1.101CysLeu: 1.101 ± 0.294
0.2CysMet: 0.2 ± 0.089
0.4CysAsn: 0.4 ± 0.148
0.45CysPro: 0.45 ± 0.178
0.3CysGln: 0.3 ± 0.122
0.651CysArg: 0.651 ± 0.201
0.601CysSer: 0.601 ± 0.201
0.501CysThr: 0.501 ± 0.161
1.001CysVal: 1.001 ± 0.262
0.1CysTrp: 0.1 ± 0.072
0.2CysTyr: 0.2 ± 0.093
0.0CysXaa: 0.0 ± 0.0
Asp
7.558AspAla: 7.558 ± 0.792
0.851AspCys: 0.851 ± 0.209
4.004AspAsp: 4.004 ± 0.473
4.354AspGlu: 4.354 ± 0.581
2.553AspPhe: 2.553 ± 0.379
5.656AspGly: 5.656 ± 0.677
0.751AspHis: 0.751 ± 0.266
3.604AspIle: 3.604 ± 0.389
3.203AspLys: 3.203 ± 0.562
5.506AspLeu: 5.506 ± 0.506
1.251AspMet: 1.251 ± 0.238
1.652AspAsn: 1.652 ± 0.294
3.904AspPro: 3.904 ± 0.592
2.302AspGln: 2.302 ± 0.343
3.704AspArg: 3.704 ± 0.413
3.303AspSer: 3.303 ± 0.546
3.704AspThr: 3.704 ± 0.479
4.204AspVal: 4.204 ± 0.469
1.101AspTrp: 1.101 ± 0.258
2.002AspTyr: 2.002 ± 0.357
0.0AspXaa: 0.0 ± 0.0
Glu
6.456GluAla: 6.456 ± 0.683
0.35GluCys: 0.35 ± 0.137
3.604GluAsp: 3.604 ± 0.424
4.054GluGlu: 4.054 ± 0.526
2.302GluPhe: 2.302 ± 0.357
2.853GluGly: 2.853 ± 0.362
1.502GluHis: 1.502 ± 0.293
3.554GluIle: 3.554 ± 0.522
2.603GluLys: 2.603 ± 0.374
7.457GluLeu: 7.457 ± 0.674
1.552GluMet: 1.552 ± 0.283
1.702GluAsn: 1.702 ± 0.284
2.903GluPro: 2.903 ± 0.407
3.103GluGln: 3.103 ± 0.461
4.705GluArg: 4.705 ± 0.619
3.353GluSer: 3.353 ± 0.388
2.202GluThr: 2.202 ± 0.321
4.555GluVal: 4.555 ± 0.521
0.901GluTrp: 0.901 ± 0.308
2.202GluTyr: 2.202 ± 0.376
0.0GluXaa: 0.0 ± 0.0
Phe
3.053PheAla: 3.053 ± 0.4
0.601PheCys: 0.601 ± 0.211
2.803PheAsp: 2.803 ± 0.368
2.352PheGlu: 2.352 ± 0.416
1.051PhePhe: 1.051 ± 0.239
2.703PheGly: 2.703 ± 0.513
0.45PheHis: 0.45 ± 0.153
1.902PheIle: 1.902 ± 0.299
1.652PheLys: 1.652 ± 0.344
2.553PheLeu: 2.553 ± 0.335
0.851PheMet: 0.851 ± 0.184
1.301PheAsn: 1.301 ± 0.269
1.552PhePro: 1.552 ± 0.266
1.101PheGln: 1.101 ± 0.203
1.852PheArg: 1.852 ± 0.318
1.451PheSer: 1.451 ± 0.314
2.402PheThr: 2.402 ± 0.335
2.903PheVal: 2.903 ± 0.493
0.551PheTrp: 0.551 ± 0.164
1.301PheTyr: 1.301 ± 0.25
0.0PheXaa: 0.0 ± 0.0
Gly
6.406GlyAla: 6.406 ± 1.035
0.851GlyCys: 0.851 ± 0.271
4.354GlyAsp: 4.354 ± 0.461
4.104GlyGlu: 4.104 ± 0.526
2.903GlyPhe: 2.903 ± 0.381
5.756GlyGly: 5.756 ± 1.01
1.001GlyHis: 1.001 ± 0.24
3.353GlyIle: 3.353 ± 0.426
2.953GlyLys: 2.953 ± 0.372
5.255GlyLeu: 5.255 ± 0.456
2.102GlyMet: 2.102 ± 0.309
3.303GlyAsn: 3.303 ± 0.503
1.502GlyPro: 1.502 ± 0.289
2.152GlyGln: 2.152 ± 0.381
4.054GlyArg: 4.054 ± 0.449
4.655GlySer: 4.655 ± 0.569
5.706GlyThr: 5.706 ± 0.704
4.955GlyVal: 4.955 ± 0.574
1.251GlyTrp: 1.251 ± 0.249
1.702GlyTyr: 1.702 ± 0.355
0.0GlyXaa: 0.0 ± 0.0
His
1.602HisAla: 1.602 ± 0.266
0.25HisCys: 0.25 ± 0.096
1.301HisAsp: 1.301 ± 0.296
1.051HisGlu: 1.051 ± 0.246
0.801HisPhe: 0.801 ± 0.176
1.301HisGly: 1.301 ± 0.235
0.501HisHis: 0.501 ± 0.161
1.101HisIle: 1.101 ± 0.274
0.751HisLys: 0.751 ± 0.222
1.351HisLeu: 1.351 ± 0.279
0.951HisMet: 0.951 ± 0.25
0.851HisAsn: 0.851 ± 0.184
0.801HisPro: 0.801 ± 0.205
0.35HisGln: 0.35 ± 0.145
0.701HisArg: 0.701 ± 0.189
0.601HisSer: 0.601 ± 0.161
0.551HisThr: 0.551 ± 0.221
1.101HisVal: 1.101 ± 0.248
0.3HisTrp: 0.3 ± 0.121
0.551HisTyr: 0.551 ± 0.197
0.0HisXaa: 0.0 ± 0.0
Ile
5.506IleAla: 5.506 ± 0.49
0.701IleCys: 0.701 ± 0.218
4.254IleAsp: 4.254 ± 0.47
4.104IleGlu: 4.104 ± 0.534
1.101IlePhe: 1.101 ± 0.254
3.403IleGly: 3.403 ± 0.444
0.751IleHis: 0.751 ± 0.187
2.102IleIle: 2.102 ± 0.326
1.852IleLys: 1.852 ± 0.315
3.453IleLeu: 3.453 ± 0.414
1.001IleMet: 1.001 ± 0.243
2.202IleAsn: 2.202 ± 0.358
2.302IlePro: 2.302 ± 0.294
2.152IleGln: 2.152 ± 0.291
2.803IleArg: 2.803 ± 0.381
2.703IleSer: 2.703 ± 0.374
3.654IleThr: 3.654 ± 0.457
3.754IleVal: 3.754 ± 0.446
0.45IleTrp: 0.45 ± 0.165
1.552IleTyr: 1.552 ± 0.363
0.0IleXaa: 0.0 ± 0.0
Lys
5.255LysAla: 5.255 ± 0.756
0.45LysCys: 0.45 ± 0.165
2.603LysAsp: 2.603 ± 0.416
1.752LysGlu: 1.752 ± 0.427
2.152LysPhe: 2.152 ± 0.338
2.152LysGly: 2.152 ± 0.403
1.301LysHis: 1.301 ± 0.292
1.952LysIle: 1.952 ± 0.34
1.952LysLys: 1.952 ± 0.424
4.304LysLeu: 4.304 ± 0.629
1.151LysMet: 1.151 ± 0.223
1.552LysAsn: 1.552 ± 0.322
2.803LysPro: 2.803 ± 0.479
2.052LysGln: 2.052 ± 0.375
3.253LysArg: 3.253 ± 0.559
2.252LysSer: 2.252 ± 0.367
3.353LysThr: 3.353 ± 0.565
2.953LysVal: 2.953 ± 0.37
0.701LysTrp: 0.701 ± 0.197
1.351LysTyr: 1.351 ± 0.312
0.0LysXaa: 0.0 ± 0.0
Leu
9.459LeuAla: 9.459 ± 0.717
0.801LeuCys: 0.801 ± 0.198
6.356LeuAsp: 6.356 ± 0.811
5.155LeuGlu: 5.155 ± 0.478
3.003LeuPhe: 3.003 ± 0.426
5.756LeuGly: 5.756 ± 0.787
1.351LeuHis: 1.351 ± 0.257
3.804LeuIle: 3.804 ± 0.477
4.254LeuLys: 4.254 ± 0.489
6.507LeuLeu: 6.507 ± 0.575
2.252LeuMet: 2.252 ± 0.294
3.504LeuAsn: 3.504 ± 0.437
4.354LeuPro: 4.354 ± 0.47
3.103LeuGln: 3.103 ± 0.349
6.557LeuArg: 6.557 ± 0.747
5.405LeuSer: 5.405 ± 0.601
5.055LeuThr: 5.055 ± 0.548
5.806LeuVal: 5.806 ± 0.612
0.851LeuTrp: 0.851 ± 0.182
2.352LeuTyr: 2.352 ± 0.404
0.0LeuXaa: 0.0 ± 0.0
Met
2.953MetAla: 2.953 ± 0.407
0.2MetCys: 0.2 ± 0.104
1.652MetAsp: 1.652 ± 0.233
1.401MetGlu: 1.401 ± 0.271
0.701MetPhe: 0.701 ± 0.219
1.101MetGly: 1.101 ± 0.274
0.4MetHis: 0.4 ± 0.137
0.901MetIle: 0.901 ± 0.229
1.101MetLys: 1.101 ± 0.271
2.202MetLeu: 2.202 ± 0.307
0.45MetMet: 0.45 ± 0.149
1.251MetAsn: 1.251 ± 0.27
1.552MetPro: 1.552 ± 0.264
1.502MetGln: 1.502 ± 0.338
3.153MetArg: 3.153 ± 0.369
2.603MetSer: 2.603 ± 0.291
1.451MetThr: 1.451 ± 0.287
1.502MetVal: 1.502 ± 0.311
0.3MetTrp: 0.3 ± 0.126
0.601MetTyr: 0.601 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
3.554AsnAla: 3.554 ± 0.413
0.4AsnCys: 0.4 ± 0.199
2.653AsnAsp: 2.653 ± 0.48
2.152AsnGlu: 2.152 ± 0.258
1.201AsnPhe: 1.201 ± 0.269
3.754AsnGly: 3.754 ± 0.493
0.701AsnHis: 0.701 ± 0.199
2.002AsnIle: 2.002 ± 0.344
1.552AsnLys: 1.552 ± 0.371
2.603AsnLeu: 2.603 ± 0.314
1.101AsnMet: 1.101 ± 0.241
1.502AsnAsn: 1.502 ± 0.302
2.953AsnPro: 2.953 ± 0.397
1.852AsnGln: 1.852 ± 0.323
2.653AsnArg: 2.653 ± 0.384
2.002AsnSer: 2.002 ± 0.391
2.603AsnThr: 2.603 ± 0.297
2.302AsnVal: 2.302 ± 0.352
0.551AsnTrp: 0.551 ± 0.172
1.301AsnTyr: 1.301 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
5.856ProAla: 5.856 ± 0.982
0.35ProCys: 0.35 ± 0.144
4.204ProAsp: 4.204 ± 0.514
3.604ProGlu: 3.604 ± 0.431
1.401ProPhe: 1.401 ± 0.273
3.403ProGly: 3.403 ± 0.489
0.801ProHis: 0.801 ± 0.211
2.052ProIle: 2.052 ± 0.299
1.952ProLys: 1.952 ± 0.335
3.754ProLeu: 3.754 ± 0.463
1.251ProMet: 1.251 ± 0.229
2.302ProAsn: 2.302 ± 0.317
2.603ProPro: 2.603 ± 0.404
1.902ProGln: 1.902 ± 0.372
2.503ProArg: 2.503 ± 0.337
3.353ProSer: 3.353 ± 0.452
3.253ProThr: 3.253 ± 0.429
3.954ProVal: 3.954 ± 0.436
0.701ProTrp: 0.701 ± 0.218
1.351ProTyr: 1.351 ± 0.249
0.0ProXaa: 0.0 ± 0.0
Gln
4.855GlnAla: 4.855 ± 0.563
0.501GlnCys: 0.501 ± 0.189
1.902GlnAsp: 1.902 ± 0.369
2.302GlnGlu: 2.302 ± 0.431
1.702GlnPhe: 1.702 ± 0.254
1.952GlnGly: 1.952 ± 0.37
0.45GlnHis: 0.45 ± 0.144
2.402GlnIle: 2.402 ± 0.402
1.502GlnLys: 1.502 ± 0.282
4.805GlnLeu: 4.805 ± 0.582
1.251GlnMet: 1.251 ± 0.248
1.151GlnAsn: 1.151 ± 0.242
2.753GlnPro: 2.753 ± 0.902
3.253GlnGln: 3.253 ± 0.683
2.903GlnArg: 2.903 ± 0.472
2.653GlnSer: 2.653 ± 0.452
2.302GlnThr: 2.302 ± 0.316
1.852GlnVal: 1.852 ± 0.319
0.551GlnTrp: 0.551 ± 0.154
1.401GlnTyr: 1.401 ± 0.267
0.0GlnXaa: 0.0 ± 0.0
Arg
7.708ArgAla: 7.708 ± 0.849
0.651ArgCys: 0.651 ± 0.231
3.754ArgAsp: 3.754 ± 0.433
4.454ArgGlu: 4.454 ± 0.561
2.503ArgPhe: 2.503 ± 0.381
4.605ArgGly: 4.605 ± 0.42
1.101ArgHis: 1.101 ± 0.198
3.453ArgIle: 3.453 ± 0.464
2.853ArgLys: 2.853 ± 0.446
5.255ArgLeu: 5.255 ± 0.565
1.952ArgMet: 1.952 ± 0.262
2.352ArgAsn: 2.352 ± 0.352
3.003ArgPro: 3.003 ± 0.463
2.853ArgGln: 2.853 ± 0.651
5.506ArgArg: 5.506 ± 0.715
3.403ArgSer: 3.403 ± 0.497
4.204ArgThr: 4.204 ± 0.572
4.204ArgVal: 4.204 ± 0.456
0.801ArgTrp: 0.801 ± 0.247
2.152ArgTyr: 2.152 ± 0.318
0.0ArgXaa: 0.0 ± 0.0
Ser
5.105SerAla: 5.105 ± 0.658
0.551SerCys: 0.551 ± 0.229
3.053SerAsp: 3.053 ± 0.447
3.554SerGlu: 3.554 ± 0.422
1.652SerPhe: 1.652 ± 0.309
3.904SerGly: 3.904 ± 0.476
1.051SerHis: 1.051 ± 0.226
3.103SerIle: 3.103 ± 0.387
2.503SerLys: 2.503 ± 0.409
5.355SerLeu: 5.355 ± 0.506
2.202SerMet: 2.202 ± 0.392
2.803SerAsn: 2.803 ± 0.439
3.153SerPro: 3.153 ± 0.431
1.802SerGln: 1.802 ± 0.321
3.804SerArg: 3.804 ± 0.457
3.353SerSer: 3.353 ± 0.592
3.904SerThr: 3.904 ± 0.439
3.854SerVal: 3.854 ± 0.484
0.601SerTrp: 0.601 ± 0.196
1.802SerTyr: 1.802 ± 0.314
0.0SerXaa: 0.0 ± 0.0
Thr
6.406ThrAla: 6.406 ± 0.783
0.45ThrCys: 0.45 ± 0.135
3.253ThrAsp: 3.253 ± 0.447
2.703ThrGlu: 2.703 ± 0.402
2.152ThrPhe: 2.152 ± 0.397
5.105ThrGly: 5.105 ± 0.473
1.001ThrHis: 1.001 ± 0.208
3.453ThrIle: 3.453 ± 0.477
3.604ThrLys: 3.604 ± 0.678
5.506ThrLeu: 5.506 ± 0.699
1.552ThrMet: 1.552 ± 0.27
2.553ThrAsn: 2.553 ± 0.393
3.504ThrPro: 3.504 ± 0.395
2.002ThrGln: 2.002 ± 0.307
3.253ThrArg: 3.253 ± 0.384
3.854ThrSer: 3.854 ± 0.555
4.104ThrThr: 4.104 ± 0.475
4.955ThrVal: 4.955 ± 0.441
0.901ThrTrp: 0.901 ± 0.223
2.252ThrTyr: 2.252 ± 0.306
0.0ThrXaa: 0.0 ± 0.0
Val
6.657ValAla: 6.657 ± 0.715
0.651ValCys: 0.651 ± 0.193
5.005ValAsp: 5.005 ± 0.44
5.155ValGlu: 5.155 ± 0.682
1.802ValPhe: 1.802 ± 0.333
3.554ValGly: 3.554 ± 0.448
1.001ValHis: 1.001 ± 0.25
2.803ValIle: 2.803 ± 0.379
2.853ValLys: 2.853 ± 0.557
5.956ValLeu: 5.956 ± 0.531
1.201ValMet: 1.201 ± 0.214
3.403ValAsn: 3.403 ± 0.458
4.354ValPro: 4.354 ± 0.515
3.303ValGln: 3.303 ± 0.444
4.555ValArg: 4.555 ± 0.687
3.754ValSer: 3.754 ± 0.402
4.354ValThr: 4.354 ± 0.433
4.204ValVal: 4.204 ± 0.447
0.801ValTrp: 0.801 ± 0.211
2.102ValTyr: 2.102 ± 0.399
0.0ValXaa: 0.0 ± 0.0
Trp
1.101TrpAla: 1.101 ± 0.227
0.35TrpCys: 0.35 ± 0.137
0.601TrpAsp: 0.601 ± 0.156
0.45TrpGlu: 0.45 ± 0.162
0.601TrpPhe: 0.601 ± 0.189
0.701TrpGly: 0.701 ± 0.182
0.4TrpHis: 0.4 ± 0.138
0.751TrpIle: 0.751 ± 0.191
0.751TrpLys: 0.751 ± 0.239
1.151TrpLeu: 1.151 ± 0.256
0.501TrpMet: 0.501 ± 0.167
0.801TrpAsn: 0.801 ± 0.206
0.801TrpPro: 0.801 ± 0.188
0.45TrpGln: 0.45 ± 0.145
0.501TrpArg: 0.501 ± 0.16
1.151TrpSer: 1.151 ± 0.25
0.551TrpThr: 0.551 ± 0.127
0.851TrpVal: 0.851 ± 0.248
0.3TrpTrp: 0.3 ± 0.12
0.4TrpTyr: 0.4 ± 0.131
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.152TyrAla: 2.152 ± 0.425
0.651TyrCys: 0.651 ± 0.195
2.352TyrAsp: 2.352 ± 0.34
1.902TyrGlu: 1.902 ± 0.317
1.301TyrPhe: 1.301 ± 0.274
2.302TyrGly: 2.302 ± 0.345
0.45TyrHis: 0.45 ± 0.162
1.952TyrIle: 1.952 ± 0.272
1.451TyrLys: 1.451 ± 0.353
2.202TyrLeu: 2.202 ± 0.335
1.001TyrMet: 1.001 ± 0.271
0.951TyrAsn: 0.951 ± 0.262
1.351TyrPro: 1.351 ± 0.269
1.051TyrGln: 1.051 ± 0.214
2.302TyrArg: 2.302 ± 0.401
1.552TyrSer: 1.552 ± 0.293
2.002TyrThr: 2.002 ± 0.335
1.752TyrVal: 1.752 ± 0.269
0.35TyrTrp: 0.35 ± 0.113
0.851TyrTyr: 0.851 ± 0.217
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 88 proteins (19981 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski